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The map label for this gene is gatB

Identifier: 110680511

GI number: 110680511

Start: 3197980

End: 3199491

Strand: Direct

Name: gatB

Synonym: RD1_3333

Alternate gene names: 110680511

Gene position: 3197980-3199491 (Clockwise)

Preceding gene: 110680510

Following gene: 110680512

Centisome position: 77.37

GC content: 59.33

Gene sequence:

>1512_bases
ATGCTCGATCTGACTTATGAGACCCCCGCACCCAAGGTGATTTCCGGTGCGAAGGAAGACTGGGAACTGGTCATCGGCAT
GGAGGTGCACGCGCAGGTCACCTCGGCTGCGAAGCTGTTTTCCGCCGCCTCCACCCAATTCGGGGCCGAGCCGAACAATA
ATGTATCCTTCGTTGATGCGGCCATGCCCGGTATGCTGCCCACGATCAACGAATACTGTGTTGAACAGGCGGTGCGCACC
GGGCTGGGCCTGAAGGCAGAAATCAACCTGCACAGCGCTTTTGACCGCAAGAACTATTTTTACCCGGATTTGCCACAGGG
TTATCAGATCAGCCAGCTCTACCACCCGATTGTCGGTGAAGGCGAAGTGCTCGTCGAAATGGGCAACGGCACGGCCCGTC
TGGTGCGGGTCGAACGCATTCACCTTGAACAGGACGCAGGCAAATCGATCCATGATATGGACCCGAACATGTCCTTTGTG
GACCTGAACCGCACCGGCGTCGCCCTGATGGAGATCGTCTCGCGCCCCGACATCCGCGGCCCCGAAGAAGCGGCGGCCTA
TCTGGGCAAACTGCGGCAGATCCTGCGCTATCTCGGCACCTGCAACGGTGACATGCAGTCGGGCGCCATGCGCGCGGACG
TCAACGTGTCGATCTGCCGGCCCGGCCAGTACGAAAAATATCAGGCCACTCAGGACTTCAGCCATCTGGGCACGCGCTGC
GAGATCAAGAACATGAACTCCATGCGGTTCATCCAACAGGCCATCGAGGTCGAGGCGCGCCGCCAGATCGCGATCGTCGA
GGCTGGCGGTACGGTTGATCAGGAAACCCGCCTTTATGATCCTGACCGCAACGAGACACGCTCCATGCGCTCCAAGGAAG
AGGCGCATGACTACCGCTATTTCCCCGACCCCGACCTGTTGCCGCTCGAAATCGAACAGGCATGGGTGGATGACATCGCC
GCCAACCTGCCCGAACTGCCTGACGCCAAGAAGGCGCGGTTTCGCAATGATTTCGGTCTGTCGGATTATGACGCTTCGGT
GCTGACCGCCGATCTGGATTCAGCGGGTTATTTCGAGGCCGTGGCGGCAGGCCGGGATGGCAAGATGGCGGCCAATTGGG
TGATCAACGAACTCTTCGGGCGGCTCAAGAAAGAGGACCACGCCATCACGGACAGCCCCGTCAGCCCTGCACAATTGGGC
GGCATTATCGATCTGATCGCCTCGGATGCGATCTCCGGCAAGATCGCCAAGGATGTCTTTGAAATCTGCTATACCACCGG
GCGCGACCCGGCGGAGATCGTGGAAACCGAAGGCATGAAACAGGTCACTGACACAGGCGCAATCGAGGCTGCCGTGGATG
AAATCATCGCCGCCAACCCCGACCAGGTGGCCAAGGCGCGCGAAAACCCGAAACTTGCGGGCTGGTTCGTCGGTCAGGTA
ATGAAAGCGACAGGCGGCAAGGCCAACCCCAAAGTGGTCAACCAACTGATTGCAAAAAGGCTTGCAGAGTAA

Upstream 100 bases:

>100_bases
GGCATGCGGCGCGCGCGGTTGCGCACAACAGACACTTGCAGCAGATGGCCCACTGGCCTAAACCCTCGGTCCATGACGAC
TGGCCCGAGGGAGACTGCCC

Downstream 100 bases:

>100_bases
TTCCCCTGCCTCAGCGCGGGGTCATTTGGTAGAATGAACCACCCGGGACAGAGGGCAAAAGCGGGGCTGACGCGCGCGAG
CCGCGCAGGCCCAAGCGATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MLDLTYETPAPKVISGAKEDWELVIGMEVHAQVTSAAKLFSAASTQFGAEPNNNVSFVDAAMPGMLPTINEYCVEQAVRT
GLGLKAEINLHSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVEMGNGTARLVRVERIHLEQDAGKSIHDMDPNMSFV
DLNRTGVALMEIVSRPDIRGPEEAAAYLGKLRQILRYLGTCNGDMQSGAMRADVNVSICRPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGTVDQETRLYDPDRNETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIA
ANLPELPDAKKARFRNDFGLSDYDASVLTADLDSAGYFEAVAAGRDGKMAANWVINELFGRLKKEDHAITDSPVSPAQLG
GIIDLIASDAISGKIAKDVFEICYTTGRDPAEIVETEGMKQVTDTGAIEAAVDEIIAANPDQVAKARENPKLAGWFVGQV
MKATGGKANPKVVNQLIAKRLAE

Sequences:

>Translated_503_residues
MLDLTYETPAPKVISGAKEDWELVIGMEVHAQVTSAAKLFSAASTQFGAEPNNNVSFVDAAMPGMLPTINEYCVEQAVRT
GLGLKAEINLHSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVEMGNGTARLVRVERIHLEQDAGKSIHDMDPNMSFV
DLNRTGVALMEIVSRPDIRGPEEAAAYLGKLRQILRYLGTCNGDMQSGAMRADVNVSICRPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGTVDQETRLYDPDRNETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIA
ANLPELPDAKKARFRNDFGLSDYDASVLTADLDSAGYFEAVAAGRDGKMAANWVINELFGRLKKEDHAITDSPVSPAQLG
GIIDLIASDAISGKIAKDVFEICYTTGRDPAEIVETEGMKQVTDTGAIEAAVDEIIAANPDQVAKARENPKLAGWFVGQV
MKATGGKANPKVVNQLIAKRLAE
>Mature_503_residues
MLDLTYETPAPKVISGAKEDWELVIGMEVHAQVTSAAKLFSAASTQFGAEPNNNVSFVDAAMPGMLPTINEYCVEQAVRT
GLGLKAEINLHSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVEMGNGTARLVRVERIHLEQDAGKSIHDMDPNMSFV
DLNRTGVALMEIVSRPDIRGPEEAAAYLGKLRQILRYLGTCNGDMQSGAMRADVNVSICRPGQYEKYQATQDFSHLGTRC
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGTVDQETRLYDPDRNETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIA
ANLPELPDAKKARFRNDFGLSDYDASVLTADLDSAGYFEAVAAGRDGKMAANWVINELFGRLKKEDHAITDSPVSPAQLG
GIIDLIASDAISGKIAKDVFEICYTTGRDPAEIVETEGMKQVTDTGAIEAAVDEIIAANPDQVAKARENPKLAGWFVGQV
MKATGGKANPKVVNQLIAKRLAE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=508, Percent_Identity=37.5984251968504, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI115534094, Length=332, Percent_Identity=37.0481927710843, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=432, Percent_Identity=30.5555555555556, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI45550808, Length=517, Percent_Identity=34.2359767891683, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ROSDO (Q163L1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683518.1
- ProteinModelPortal:   Q163L1
- SMR:   Q163L1
- STRING:   Q163L1
- GeneID:   4195480
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3333
- NMPDR:   fig|375451.6.peg.3100
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q163L1
- ProtClustDB:   PRK05477
- BioCyc:   RDEN375451:RD1_3333-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55322; Mature: 55322

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLTYETPAPKVISGAKEDWELVIGMEVHAQVTSAAKLFSAASTQFGAEPNNNVSFVDA
CCEECCCCCCCHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEH
AMPGMLPTINEYCVEQAVRTGLGLKAEINLHSAFDRKNYFYPDLPQGYQISQLYHPIVGE
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVEMGNGTARLVRVERIHLEQDAGKSIHDMDPNMSFVDLNRTGVALMEIVSRPDIRG
CEEEEEECCCCEEEEEEEHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCC
PEEAAAYLGKLRQILRYLGTCNGDMQSGAMRADVNVSICRPGQYEKYQATQDFSHLGTRC
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEECCEEEEECCCCCCHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGTVDQETRLYDPDRNETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
FPDPDLLPLEIEQAWVDDIAANLPELPDAKKARFRNDFGLSDYDASVLTADLDSAGYFEA
CCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHH
VAAGRDGKMAANWVINELFGRLKKEDHAITDSPVSPAQLGGIIDLIASDAISGKIAKDVF
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
EICYTTGRDPAEIVETEGMKQVTDTGAIEAAVDEIIAANPDQVAKARENPKLAGWFVGQV
HHHHHCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHH
MKATGGKANPKVVNQLIAKRLAE
HHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLTYETPAPKVISGAKEDWELVIGMEVHAQVTSAAKLFSAASTQFGAEPNNNVSFVDA
CCEECCCCCCCHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEH
AMPGMLPTINEYCVEQAVRTGLGLKAEINLHSAFDRKNYFYPDLPQGYQISQLYHPIVGE
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCC
GEVLVEMGNGTARLVRVERIHLEQDAGKSIHDMDPNMSFVDLNRTGVALMEIVSRPDIRG
CEEEEEECCCCEEEEEEEHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCC
PEEAAAYLGKLRQILRYLGTCNGDMQSGAMRADVNVSICRPGQYEKYQATQDFSHLGTRC
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEECCEEEEECCCCCCHHHHHHHHHHCCCEE
EIKNMNSMRFIQQAIEVEARRQIAIVEAGGTVDQETRLYDPDRNETRSMRSKEEAHDYRY
EECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
FPDPDLLPLEIEQAWVDDIAANLPELPDAKKARFRNDFGLSDYDASVLTADLDSAGYFEA
CCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHH
VAAGRDGKMAANWVINELFGRLKKEDHAITDSPVSPAQLGGIIDLIASDAISGKIAKDVF
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
EICYTTGRDPAEIVETEGMKQVTDTGAIEAAVDEIIAANPDQVAKARENPKLAGWFVGQV
HHHHHCCCCHHHHHHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHH
MKATGGKANPKVVNQLIAKRLAE
HHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA