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The map label for this gene is pcm

Identifier: 110680430

GI number: 110680430

Start: 3117943

End: 3118584

Strand: Direct

Name: pcm

Synonym: RD1_3250

Alternate gene names: 110680430

Gene position: 3117943-3118584 (Clockwise)

Preceding gene: 110680429

Following gene: 110680431

Centisome position: 75.44

GC content: 60.9

Gene sequence:

>642_bases
ATGTCTGAGGCAGAACGCAAGATGCAGTTCCTCTATGCCCTGCGCTCCAAGGGTGTCACAGATAAACGCGTGCTCTCGGC
CATGGAAAACATCGACCGTGGCCCCTTCATCCGGGGGATTTTTGCCGAGCGCGCCTATGAGGACATGCCGCTGCCGATTG
CCTGCGGTCAGACCATCAGCCAGCCATCGGTTGTCGGGCTGATGACACAGGCGCTCAAGATCAGCCCGCGCGACAAGGTG
CTGGAGGTGGGCACGGGCTCCGGGTATCAGGCCGCGATCCTCAGCCGGTTGGCGCGCCGGGTCTATACGCTGGACCGCCA
CAGCCGATTGGTGCGCGAGGCGCGCACCCTTTTCGAAGAGATGAACCTCACAAATATCACCGCGATCACCGCAGATGGCA
GCTATGGCCTTGCGGATCAGGCCCCGTTTGATCGCATCCTCGTCACTGCCGCCGCCGAGGACCCCCCCAGCACGCTGATG
GAGCAGCTCAAGGTCGGCGGTATCATGGTCATTCCCGTCGGACAGTCTGACGCCGTTCAGCATCTGATCGTCGTTCACAA
GACCGAAGACGGGCTTGAGTATGACGAGCTGCGGCAAGTCCGCTTTGTTCCTTTGCTGGAAGGCTTGGGAAAGGACGGAT
AA

Upstream 100 bases:

>100_bases
TGAACCTTGAGGGGTACATCTCGGTCACGCCGATGCGGGCGGATTTGACCGCGCATGACATGATGGCCCCGCTGGCGGGC
ATCAATACATGATGACAGAA

Downstream 100 bases:

>100_bases
GCTGTTATAGTGTTAACGAATAAGCGGCCGGAAAACCGGCTCACGCTCAAAGAGCTACGTTGAGGACATGCAGATGGTCA
GACATTTTTCCCGCACGCGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSEAERKMQFLYALRSKGVTDKRVLSAMENIDRGPFIRGIFAERAYEDMPLPIACGQTISQPSVVGLMTQALKISPRDKV
LEVGTGSGYQAAILSRLARRVYTLDRHSRLVREARTLFEEMNLTNITAITADGSYGLADQAPFDRILVTAAAEDPPSTLM
EQLKVGGIMVIPVGQSDAVQHLIVVHKTEDGLEYDELRQVRFVPLLEGLGKDG

Sequences:

>Translated_213_residues
MSEAERKMQFLYALRSKGVTDKRVLSAMENIDRGPFIRGIFAERAYEDMPLPIACGQTISQPSVVGLMTQALKISPRDKV
LEVGTGSGYQAAILSRLARRVYTLDRHSRLVREARTLFEEMNLTNITAITADGSYGLADQAPFDRILVTAAAEDPPSTLM
EQLKVGGIMVIPVGQSDAVQHLIVVHKTEDGLEYDELRQVRFVPLLEGLGKDG
>Mature_212_residues
SEAERKMQFLYALRSKGVTDKRVLSAMENIDRGPFIRGIFAERAYEDMPLPIACGQTISQPSVVGLMTQALKISPRDKVL
EVGTGSGYQAAILSRLARRVYTLDRHSRLVREARTLFEEMNLTNITAITADGSYGLADQAPFDRILVTAAAEDPPSTLME
QLKVGGIMVIPVGQSDAVQHLIVVHKTEDGLEYDELRQVRFVPLLEGLGKDG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=30.6603773584906, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789100, Length=204, Percent_Identity=41.6666666666667, Blast_Score=160, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI71983477, Length=216, Percent_Identity=31.0185185185185, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=214, Percent_Identity=30.3738317757009, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17981723, Length=211, Percent_Identity=29.3838862559242, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_ROSDO (Q163U2)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683437.1
- ProteinModelPortal:   Q163U2
- SMR:   Q163U2
- STRING:   Q163U2
- GeneID:   4198560
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3250
- NMPDR:   fig|375451.6.peg.3018
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- PhylomeDB:   Q163U2
- ProtClustDB:   CLSK933941
- BioCyc:   RDEN375451:RD1_3250-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23490; Mature: 23359

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAERKMQFLYALRSKGVTDKRVLSAMENIDRGPFIRGIFAERAYEDMPLPIACGQTIS
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCCC
QPSVVGLMTQALKISPRDKVLEVGTGSGYQAAILSRLARRVYTLDRHSRLVREARTLFEE
CCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNLTNITAITADGSYGLADQAPFDRILVTAAAEDPPSTLMEQLKVGGIMVIPVGQSDAVQ
CCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCHHHE
HLIVVHKTEDGLEYDELRQVRFVPLLEGLGKDG
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEAERKMQFLYALRSKGVTDKRVLSAMENIDRGPFIRGIFAERAYEDMPLPIACGQTIS
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCCC
QPSVVGLMTQALKISPRDKVLEVGTGSGYQAAILSRLARRVYTLDRHSRLVREARTLFEE
CCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNLTNITAITADGSYGLADQAPFDRILVTAAAEDPPSTLMEQLKVGGIMVIPVGQSDAVQ
CCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCHHHE
HLIVVHKTEDGLEYDELRQVRFVPLLEGLGKDG
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA