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The map label for this gene is rihA [H]

Identifier: 110680321

GI number: 110680321

Start: 3010505

End: 3011446

Strand: Direct

Name: rihA [H]

Synonym: RD1_3134

Alternate gene names: 110680321

Gene position: 3010505-3011446 (Clockwise)

Preceding gene: 110680320

Following gene: 110680322

Centisome position: 72.84

GC content: 59.66

Gene sequence:

>942_bases
ATGACAGCACGTAAAATCATCATTGATACCGACCCCGGCCAGGACGACGCGGTGGCGATCCTTCTGGCGCTCGCCAGCCC
GGAAGAGCTTGATGTTCTCGGCATTACCTGTGTCGCCGGCAACGTCCCGCTTGACCTGACGGCGCGCAACGCGCGCATTG
TCTGTGAACTGGCCAAAAAGACCGATGTGCCGGTTTTTGCAGGCTGCGACCGCCCCCTTGGCCGCGACCTCGTCACCGCA
GAGCATGTGCATGGCAAAACCGGGCTCGATGGGCCTGACCTGCCGGAGCCGGTGATGGCTCTGCAGGACCAACACGCCGT
TGATTTCATCATCAACACCCTGCGCAACACACCACCCGGCACCGTAACGCTCTGCCCGCTGGGACCGCTGACCAACATCG
CCACCGCCTTTGAAAAAGCGCCGGATATCGTGCAGCGCGTGCAACAGATCGTCTTGATGGGCGGGGCGTATTTCGAGGTT
GGGAATATCACCCCGACCGCGGAATTCAACATTTACGTCGACCCACAAGCCGCTGAAATCACGTTTAAATCCGGCGTGGA
TATCGTCGTGCTGCCACTGGATGTCACCCACAAGGCGCTTGTCACTGCCCCGCGCAACGACGCCTTTCGCGCGCTGAACA
CGCCCGTTGGCATCGCCGTGGCCGAGATGACCGATTTCTTTGAGCGTTTTGACAAGGAAAAATACGGCTCCGCCGGGGCA
CCGCTGCATGATCCTTGTGTGACCGCCTATTTGCTGCGCCCTGATCTGTTCACCGGGCGGCACATCAATGTGGAAATCGA
AACCACGTCTGAGTTGACCATGGGCATGACCGTCGCGGACTGGTGGGGCGTGACGGATCGGCCAAAAAACGCGCTGTTCA
TCGGCGATCTGGATGCGGATGGGTTCTTTGAATTGCTGACAGAACGGCTCGCCCGGCTATGA

Upstream 100 bases:

>100_bases
CGCATGTGTATGTCGGTGCATCGGCGCCATCACGCCATGGCAGCACATTTGTCACGCATCCCCGCTGGACTCTCAACCGC
CACAGCGTCAAAGAGAGCAC

Downstream 100 bases:

>100_bases
GCGCGGCCCTGACCCTTGCAGCCCCCGATCATCTTGAGAAACTCGATGCGCTGGTGGCGGCGTTTCATCAGGAGGCCGGG
ATCACCCTCGCAGCGGAGGC

Product: inosine-uridine preferring nucleoside hydrolase, putative

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTARKIIIDTDPGQDDAVAILLALASPEELDVLGITCVAGNVPLDLTARNARIVCELAKKTDVPVFAGCDRPLGRDLVTA
EHVHGKTGLDGPDLPEPVMALQDQHAVDFIINTLRNTPPGTVTLCPLGPLTNIATAFEKAPDIVQRVQQIVLMGGAYFEV
GNITPTAEFNIYVDPQAAEITFKSGVDIVVLPLDVTHKALVTAPRNDAFRALNTPVGIAVAEMTDFFERFDKEKYGSAGA
PLHDPCVTAYLLRPDLFTGRHINVEIETTSELTMGMTVADWWGVTDRPKNALFIGDLDADGFFELLTERLARL

Sequences:

>Translated_313_residues
MTARKIIIDTDPGQDDAVAILLALASPEELDVLGITCVAGNVPLDLTARNARIVCELAKKTDVPVFAGCDRPLGRDLVTA
EHVHGKTGLDGPDLPEPVMALQDQHAVDFIINTLRNTPPGTVTLCPLGPLTNIATAFEKAPDIVQRVQQIVLMGGAYFEV
GNITPTAEFNIYVDPQAAEITFKSGVDIVVLPLDVTHKALVTAPRNDAFRALNTPVGIAVAEMTDFFERFDKEKYGSAGA
PLHDPCVTAYLLRPDLFTGRHINVEIETTSELTMGMTVADWWGVTDRPKNALFIGDLDADGFFELLTERLARL
>Mature_312_residues
TARKIIIDTDPGQDDAVAILLALASPEELDVLGITCVAGNVPLDLTARNARIVCELAKKTDVPVFAGCDRPLGRDLVTAE
HVHGKTGLDGPDLPEPVMALQDQHAVDFIINTLRNTPPGTVTLCPLGPLTNIATAFEKAPDIVQRVQQIVLMGGAYFEVG
NITPTAEFNIYVDPQAAEITFKSGVDIVVLPLDVTHKALVTAPRNDAFRALNTPVGIAVAEMTDFFERFDKEKYGSAGAP
LHDPCVTAYLLRPDLFTGRHINVEIETTSELTMGMTVADWWGVTDRPKNALFIGDLDADGFFELLTERLARL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=48.8673139158576, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=41.5584415584416, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI1786213, Length=306, Percent_Identity=38.562091503268, Blast_Score=158, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32565474, Length=200, Percent_Identity=39.5, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17565698, Length=305, Percent_Identity=27.2131147540984, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=267, Percent_Identity=35.9550561797753, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24583915, Length=205, Percent_Identity=34.6341463414634, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24641839, Length=204, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24641837, Length=201, Percent_Identity=32.3383084577114, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33885; Mature: 33754

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARKIIIDTDPGQDDAVAILLALASPEELDVLGITCVAGNVPLDLTARNARIVCELAKK
CCCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCEEEEECCCEEEEEEHHH
TDVPVFAGCDRPLGRDLVTAEHVHGKTGLDGPDLPEPVMALQDQHAVDFIINTLRNTPPG
CCCCEEECCCCCCCCCCEEHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCC
TVTLCPLGPLTNIATAFEKAPDIVQRVQQIVLMGGAYFEVGNITPTAEFNIYVDPQAAEI
EEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEE
TFKSGVDIVVLPLDVTHKALVTAPRNDAFRALNTPVGIAVAEMTDFFERFDKEKYGSAGA
EECCCCCEEEEECCCCCEEEEECCCCCCHHHHCCCCCEEHHHHHHHHHHHCHHHCCCCCC
PLHDPCVTAYLLRPDLFTGRHINVEIETTSELTMGMTVADWWGVTDRPKNALFIGDLDAD
CCCCHHHEEEEECCCCCCCCEEEEEEEECCCEEECEEEHHHCCCCCCCCCEEEEECCCCC
GFFELLTERLARL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TARKIIIDTDPGQDDAVAILLALASPEELDVLGITCVAGNVPLDLTARNARIVCELAKK
CCCEEEEECCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCEEEEECCCEEEEEEHHH
TDVPVFAGCDRPLGRDLVTAEHVHGKTGLDGPDLPEPVMALQDQHAVDFIINTLRNTPPG
CCCCEEECCCCCCCCCCEEHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCC
TVTLCPLGPLTNIATAFEKAPDIVQRVQQIVLMGGAYFEVGNITPTAEFNIYVDPQAAEI
EEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCEEE
TFKSGVDIVVLPLDVTHKALVTAPRNDAFRALNTPVGIAVAEMTDFFERFDKEKYGSAGA
EECCCCCEEEEECCCCCEEEEECCCCCCHHHHCCCCCEEHHHHHHHHHHHCHHHCCCCCC
PLHDPCVTAYLLRPDLFTGRHINVEIETTSELTMGMTVADWWGVTDRPKNALFIGDLDAD
CCCCHHHEEEEECCCCCCCCEEEEEEEECCCEEECEEEHHHCCCCCCCCCEEEEECCCCC
GFFELLTERLARL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA