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The map label for this gene is mazG [H]

Identifier: 110680320

GI number: 110680320

Start: 3009511

End: 3010326

Strand: Direct

Name: mazG [H]

Synonym: RD1_3133

Alternate gene names: 110680320

Gene position: 3009511-3010326 (Clockwise)

Preceding gene: 110680315

Following gene: 110680321

Centisome position: 72.81

GC content: 61.27

Gene sequence:

>816_bases
ATGGCAGATGACCTGATCCACGACCCTGATGGGGGAATTGAACGCCTGCTTGCGATCATGCGCCGCCTGCGTGACCCGGA
AACGGGCTGCCCGTGGGACATCGAGCAGGATTTCGACAGCATCGCCCCCTACACAATCGAAGAGGCCTATGAGGTCGCAG
ACGCCATCGCACGCAAGGACTGGCGCGATCTTGAGGGGGAGTTGGGCGATCTGCTGCTGCAAACCGTTTATCATACGGCC
ATGGGTGCCGAAGGCGGTCACTTCACCTTTCAGTCGGTGGTGCGCAAGATCAGCGACAAGATGGTCGCCCGCCACCCGCA
TGTTTTTGGCGATGAGAGCCGCGACAAGTCAGCGGACCAGCAAACCGCCGATTGGGAAAAGATAAAGGCCCGCGAACGTG
CAGAAAACGCGCAACAGGGCACGCTGGATGGTGTGGCCATTGGCCTGCCCGCGCTGCTGCGCGCGCTCAAGCTGCAAAAA
CGCGCCGCACGGGTCGGATTTGACTGGCCCTCCACCGATGAAGTCCTGGACAAAATCACCGAAGAGGCTGCCGAGTTGGT
CGAGGCGCGCGATCGTCTCACGCAAGCAGAAATCGAAGAGGAGTTCGGCGATCTGCTGTTCGTGATGGCCAATCTGGGCC
GTCACCTCGGACTTGATCCCGAAGCCGCCCTGCGCGCTGCCAACGCGAAATTCACCCGTCGTTTCGAAGGGGTCGAAGCC
AAGCTGGCCGCCATGGGTAAGACCCCCGCCGACAGCGATCTGGCTGAGATGGATGCGCTTTGGGATCAGGTCAAGGCGGA
GAGCCGGAAGCGCTAG

Upstream 100 bases:

>100_bases
CCGCGCTCATGTCAGCGATCCGGTTTACGACGGGCATGCTCTGGACTCCTTGTCGAATGTTTTGTCAGGTGCATCTGACA
CCGAAGCGGGAGCGGCTGCA

Downstream 100 bases:

>100_bases
GTCCGCGCCGGTCTTGCAGCGCCTGCTCGCAATTCGGCGCGCACAGTTGCGCCCGCCGGCGTGACGGACGCGGGGCGGCG
CATGTGTATGTCGGTGCATC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MADDLIHDPDGGIERLLAIMRRLRDPETGCPWDIEQDFDSIAPYTIEEAYEVADAIARKDWRDLEGELGDLLLQTVYHTA
MGAEGGHFTFQSVVRKISDKMVARHPHVFGDESRDKSADQQTADWEKIKARERAENAQQGTLDGVAIGLPALLRALKLQK
RAARVGFDWPSTDEVLDKITEEAAELVEARDRLTQAEIEEEFGDLLFVMANLGRHLGLDPEAALRAANAKFTRRFEGVEA
KLAAMGKTPADSDLAEMDALWDQVKAESRKR

Sequences:

>Translated_271_residues
MADDLIHDPDGGIERLLAIMRRLRDPETGCPWDIEQDFDSIAPYTIEEAYEVADAIARKDWRDLEGELGDLLLQTVYHTA
MGAEGGHFTFQSVVRKISDKMVARHPHVFGDESRDKSADQQTADWEKIKARERAENAQQGTLDGVAIGLPALLRALKLQK
RAARVGFDWPSTDEVLDKITEEAAELVEARDRLTQAEIEEEFGDLLFVMANLGRHLGLDPEAALRAANAKFTRRFEGVEA
KLAAMGKTPADSDLAEMDALWDQVKAESRKR
>Mature_270_residues
ADDLIHDPDGGIERLLAIMRRLRDPETGCPWDIEQDFDSIAPYTIEEAYEVADAIARKDWRDLEGELGDLLLQTVYHTAM
GAEGGHFTFQSVVRKISDKMVARHPHVFGDESRDKSADQQTADWEKIKARERAENAQQGTLDGVAIGLPALLRALKLQKR
AARVGFDWPSTDEVLDKITEEAAELVEARDRLTQAEIEEEFGDLLFVMANLGRHLGLDPEAALRAANAKFTRRFEGVEAK
LAAMGKTPADSDLAEMDALWDQVKAESRKR

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=255, Percent_Identity=54.9019607843137, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30313; Mature: 30182

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDLIHDPDGGIERLLAIMRRLRDPETGCPWDIEQDFDSIAPYTIEEAYEVADAIARKD
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
WRDLEGELGDLLLQTVYHTAMGAEGGHFTFQSVVRKISDKMVARHPHVFGDESRDKSADQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QTADWEKIKARERAENAQQGTLDGVAIGLPALLRALKLQKRAARVGFDWPSTDEVLDKIT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EEAAELVEARDRLTQAEIEEEFGDLLFVMANLGRHLGLDPEAALRAANAKFTRRFEGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KLAAMGKTPADSDLAEMDALWDQVKAESRKR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADDLIHDPDGGIERLLAIMRRLRDPETGCPWDIEQDFDSIAPYTIEEAYEVADAIARKD
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
WRDLEGELGDLLLQTVYHTAMGAEGGHFTFQSVVRKISDKMVARHPHVFGDESRDKSADQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
QTADWEKIKARERAENAQQGTLDGVAIGLPALLRALKLQKRAARVGFDWPSTDEVLDKIT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EEAAELVEARDRLTQAEIEEEFGDLLFVMANLGRHLGLDPEAALRAANAKFTRRFEGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KLAAMGKTPADSDLAEMDALWDQVKAESRKR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]