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The map label for this gene is ksgA

Identifier: 110680311

GI number: 110680311

Start: 3002078

End: 3002920

Strand: Direct

Name: ksgA

Synonym: RD1_3124

Alternate gene names: 110680311

Gene position: 3002078-3002920 (Clockwise)

Preceding gene: 110680310

Following gene: 110680315

Centisome position: 72.64

GC content: 62.87

Gene sequence:

>843_bases
ATGAGCACGATCGACCACCTGCCCCCCTTGCGCAGCGTCATCCGGGATCATGATCTGTCGGCGCGTAAATCCATGGGACA
GAACTTTCTGCTGGATTTGAACCTGACGGCCAAGATTGCACGTCAGGCCGGGGATCTGTCCGCCTGTGATGTGTTGGAGA
TCGGCCCGGGGCCCGGTGGTCTGACCCGTGGGCTTCTGGCGCAAGGCGCGCGGCGCGTTCTGGCCATTGAAAAAGACGCC
CGTTGCCTGCCCGCTCTTGCCGAGATTGCAGCGGTCTATCCCGGACGGCTGGAGGTCATGAACGGCGACGCGCTGGAGAT
CGACCCGCTGAGCGCCCTGACACCGCCCATCCGGGTCGCTGCCAACCTGCCCTATAACGTGGGGACCGAGCTTTTGGTGC
GCTGGTTGACGCCGCAGATATGGCCACCCTACTGGCAAAGCTTGACGCTCATGTTCCAGCGCGAAGTGGCAGAACGTATC
GTGGCCAGACCGGGCAGCAAAGCCTATGGACGCCTTGCCATTCTGGCGCAATGGCGTGCAGATGCACGGATTGTGATGCA
ATTGCCGCCAGACGCCTTCACGCCGCCGCCCAAAGTTTCAAGCAGCGTGGTGCAGATCACCGCCCTGCCCGCACCGCGGT
ATCCGGCCGACCCGTATGTGCTGAGCAAAGTGGTTGCGATGGCGTTCAATCAGCGCCGCAAGATGCTGCGCGCGGCCCTG
AAGGGCCTTGGCCCGGATATCGAGGATCGGCTTTTGGCAGCCGGTATAGAACCCACCGAACGCGCGGAACGTGTCAGCCT
CGAAGGGTTTTGTGCTTTGGCGCGGGCCGTTGCCGCGACATAG

Upstream 100 bases:

>100_bases
GGGCATCGCCAATCCGACCAGCATGATCGAGGCGATCAAGCTGGCCGCGCATATGGCGGCCCGGCATGTCTGACGGGACG
CCCTGCGGGTGCAGGCGCCC

Downstream 100 bases:

>100_bases
CACGCGCGAGCCGACCAGACGTAATCGCCTATTCCGCCGCCTTTGGTGCGTCATCCCCGTCGGAGGAAGAAGACGCCGCC
TCGCCGCTCTCTTCTGCGGC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSTIDHLPPLRSVIRDHDLSARKSMGQNFLLDLNLTAKIARQAGDLSACDVLEIGPGPGGLTRGLLAQGARRVLAIEKDA
RCLPALAEIAAVYPGRLEVMNGDALEIDPLSALTPPIRVAANLPYNVGTELLVRWLTPQIWPPYWQSLTLMFQREVAERI
VARPGSKAYGRLAILAQWRADARIVMQLPPDAFTPPPKVSSSVVQITALPAPRYPADPYVLSKVVAMAFNQRRKMLRAAL
KGLGPDIEDRLLAAGIEPTERAERVSLEGFCALARAVAAT

Sequences:

>Translated_280_residues
MSTIDHLPPLRSVIRDHDLSARKSMGQNFLLDLNLTAKIARQAGDLSACDVLEIGPGPGGLTRGLLAQGARRVLAIEKDA
RCLPALAEIAAVYPGRLEVMNGDALEIDPLSALTPPIRVAANLPYNVGTELLVRWLTPQIWPPYWQSLTLMFQREVAERI
VARPGSKAYGRLAILAQWRADARIVMQLPPDAFTPPPKVSSSVVQITALPAPRYPADPYVLSKVVAMAFNQRRKMLRAAL
KGLGPDIEDRLLAAGIEPTERAERVSLEGFCALARAVAAT
>Mature_279_residues
STIDHLPPLRSVIRDHDLSARKSMGQNFLLDLNLTAKIARQAGDLSACDVLEIGPGPGGLTRGLLAQGARRVLAIEKDAR
CLPALAEIAAVYPGRLEVMNGDALEIDPLSALTPPIRVAANLPYNVGTELLVRWLTPQIWPPYWQSLTLMFQREVAERIV
ARPGSKAYGRLAILAQWRADARIVMQLPPDAFTPPPKVSSSVVQITALPAPRYPADPYVLSKVVAMAFNQRRKMLRAALK
GLGPDIEDRLLAAGIEPTERAERVSLEGFCALARAVAAT

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=36.6438356164384, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI7657198, Length=197, Percent_Identity=37.0558375634518, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1786236, Length=264, Percent_Identity=35.9848484848485, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=32.3232323232323, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146882, Length=281, Percent_Identity=30.2491103202847, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324989, Length=195, Percent_Identity=33.8461538461538, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357273, Length=256, Percent_Identity=33.984375, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21358017, Length=236, Percent_Identity=34.7457627118644, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ROSDO (Q164G1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683318.1
- ProteinModelPortal:   Q164G1
- SMR:   Q164G1
- STRING:   Q164G1
- GeneID:   4198669
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3124
- NMPDR:   fig|375451.6.peg.2899
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q164G1
- ProtClustDB:   PRK00274
- BioCyc:   RDEN375451:RD1_3124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30419; Mature: 30288

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIDHLPPLRSVIRDHDLSARKSMGQNFLLDLNLTAKIARQAGDLSACDVLEIGPGPGG
CCCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCEEEECCCCCH
LTRGLLAQGARRVLAIEKDARCLPALAEIAAVYPGRLEVMNGDALEIDPLSALTPPIRVA
HHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHCCCCCEEE
ANLPYNVGTELLVRWLTPQIWPPYWQSLTLMFQREVAERIVARPGSKAYGRLAILAQWRA
ECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEECCC
DARIVMQLPPDAFTPPPKVSSSVVQITALPAPRYPADPYVLSKVVAMAFNQRRKMLRAAL
CCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGLGPDIEDRLLAAGIEPTERAERVSLEGFCALARAVAAT
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STIDHLPPLRSVIRDHDLSARKSMGQNFLLDLNLTAKIARQAGDLSACDVLEIGPGPGG
CCCCCCHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCEEEECCCCCH
LTRGLLAQGARRVLAIEKDARCLPALAEIAAVYPGRLEVMNGDALEIDPLSALTPPIRVA
HHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHCCCCCEEE
ANLPYNVGTELLVRWLTPQIWPPYWQSLTLMFQREVAERIVARPGSKAYGRLAILAQWRA
ECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEECCC
DARIVMQLPPDAFTPPPKVSSSVVQITALPAPRYPADPYVLSKVVAMAFNQRRKMLRAAL
CCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGLGPDIEDRLLAAGIEPTERAERVSLEGFCALARAVAAT
HHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA