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The map label for this gene is gltD [H]

Identifier: 110680293

GI number: 110680293

Start: 2981292

End: 2982626

Strand: Reverse

Name: gltD [H]

Synonym: RD1_3104

Alternate gene names: 110680293

Gene position: 2982626-2981292 (Counterclockwise)

Preceding gene: 110680294

Following gene: 110680292

Centisome position: 72.16

GC content: 61.65

Gene sequence:

>1335_bases
ATGATTAGCCAACGTACACCGGGCATCGTCGCCGAAAGGCTGAGCGCAGAAGAACTGGCCCGTAATTTTGCCGACCTTCA
CGCACCTCTGGAGCCACATGAAGCAGCAGTTGCTGCGGATCGCTGTTATTTCTGCCATGACGCGCCCTGCGTGACGGCCT
GTCCCACGGAAATCGACATCCCGCTCTTCATCCGCCAGATCAGCACAGGCACCCCCGAAGCAGCAGCCAAAACGATTTTT
GACCAGAACATTCTGGGCGGCATGTGCGCGCGGGTCTGTCCGACCGAAGATCTGTGCGAGCAGGCCTGCGTGCGTGAAAT
GGCAGAGGGCAAACCGGTCGAGATCGGGCGCCTGCAACGCTATGCGACCGATACGCTGATGCAGCGCGACATTCACCCCT
ATACCCGTGCGGCGGCCACGGGAAAGCGGATCGCGGTTGTGGGCGCGGGGCCTGCTGGTCTTTCTTGCGCGCACAGGTTG
GCGCTGCTTGGCAATGATGTTGTAGTCTATGATGGCCACGCCAAACCGGGCGGTCTGAATGAATATGGGATCGCAGCCTA
CAAATCCACGGATGATTTTGCCGCGCGCGAGGTCGCGTGGCTGTTGGACATCGGTGGCATCACGGTTGAAACGGGCAAGG
TGTTGGGCGGTGATCTGGCGCTGGATGCTTTGGCGGCGCAATATGACGCTGTGTTTCTGGCCGTGGGTCTGGGCGGGGTG
AACGCGCTCAATCTTGAGGGGGATGACAAATCCGGCGTCACGGACGCGGTGTCTTTCATCGAAACGCTGCGCCAGTCGCG
CGATCTGACCAAGCTGCCCGTGGGCCGCAATGTGGTGGTCATCGGCGGGGGCATGACGGCCGTGGACGCTGCGGTTCAGT
CCAAATTGCTGGGCGCGGAGAATGTCACGATCGCCTATCGGCGCGACCGCGCCGCGATGAGTGCCAGCCGCTACGAACAG
GACCTTGCCGCCTCCCATGGCGTCAAGCTGATGTTCAATGTGCAGCCCGTCGCCATTCATGGCAACGGGGCAGCGGCGGA
GATCGAACTGGAATATACCGCATCGGTTGATGGCCGACTTGTCGGGACAGGCGAAAAGACGCGCCTGACGGCGGATCAGG
TATTCCGTGCCATCGGGCAGACGCTGACCATGGAAGGGGGGCTTGAAACAAGGAACGGCAAGATCGCTGTGTCTGGTGCA
GGGCGGACCAGTCTGGACCGGGTCTGGGCCGGGGGCGATTGCGCCCTTGGCGGCGATGATCTGACCGTGACAGCCGTGGC
CGAAGGGCGCGACGCGGCAATGGATATTCATGCAGCACTGAACGGGAGCGTATAA

Upstream 100 bases:

>100_bases
CCTGCGATTGAAAAAACCTTGCCAAAAAAAATTTTTTACCGTTTGATAAAATTTTAAACCATGTAATGCTGATGACATAG
CAAAACCCGACGGAGTAGCC

Downstream 100 bases:

>100_bases
TGGCTGATCTGACGTCGGAATTTCTGGGCATTACCTCACCAAATCCGTTTTGGCTGGCCTCTGCGCCGCCAACTGACAAG
GAATACAACGTGCGCCGCGC

Product: glutamate synthase subunit beta

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MISQRTPGIVAERLSAEELARNFADLHAPLEPHEAAVAADRCYFCHDAPCVTACPTEIDIPLFIRQISTGTPEAAAKTIF
DQNILGGMCARVCPTEDLCEQACVREMAEGKPVEIGRLQRYATDTLMQRDIHPYTRAAATGKRIAVVGAGPAGLSCAHRL
ALLGNDVVVYDGHAKPGGLNEYGIAAYKSTDDFAAREVAWLLDIGGITVETGKVLGGDLALDALAAQYDAVFLAVGLGGV
NALNLEGDDKSGVTDAVSFIETLRQSRDLTKLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRDRAAMSASRYEQ
DLAASHGVKLMFNVQPVAIHGNGAAAEIELEYTASVDGRLVGTGEKTRLTADQVFRAIGQTLTMEGGLETRNGKIAVSGA
GRTSLDRVWAGGDCALGGDDLTVTAVAEGRDAAMDIHAALNGSV

Sequences:

>Translated_444_residues
MISQRTPGIVAERLSAEELARNFADLHAPLEPHEAAVAADRCYFCHDAPCVTACPTEIDIPLFIRQISTGTPEAAAKTIF
DQNILGGMCARVCPTEDLCEQACVREMAEGKPVEIGRLQRYATDTLMQRDIHPYTRAAATGKRIAVVGAGPAGLSCAHRL
ALLGNDVVVYDGHAKPGGLNEYGIAAYKSTDDFAAREVAWLLDIGGITVETGKVLGGDLALDALAAQYDAVFLAVGLGGV
NALNLEGDDKSGVTDAVSFIETLRQSRDLTKLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRDRAAMSASRYEQ
DLAASHGVKLMFNVQPVAIHGNGAAAEIELEYTASVDGRLVGTGEKTRLTADQVFRAIGQTLTMEGGLETRNGKIAVSGA
GRTSLDRVWAGGDCALGGDDLTVTAVAEGRDAAMDIHAALNGSV
>Mature_444_residues
MISQRTPGIVAERLSAEELARNFADLHAPLEPHEAAVAADRCYFCHDAPCVTACPTEIDIPLFIRQISTGTPEAAAKTIF
DQNILGGMCARVCPTEDLCEQACVREMAEGKPVEIGRLQRYATDTLMQRDIHPYTRAAATGKRIAVVGAGPAGLSCAHRL
ALLGNDVVVYDGHAKPGGLNEYGIAAYKSTDDFAAREVAWLLDIGGITVETGKVLGGDLALDALAAQYDAVFLAVGLGGV
NALNLEGDDKSGVTDAVSFIETLRQSRDLTKLPVGRNVVVIGGGMTAVDAAVQSKLLGAENVTIAYRRDRAAMSASRYEQ
DLAASHGVKLMFNVQPVAIHGNGAAAEIELEYTASVDGRLVGTGEKTRLTADQVFRAIGQTLTMEGGLETRNGKIAVSGA
GRTSLDRVWAGGDCALGGDDLTVTAVAEGRDAAMDIHAALNGSV

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=466, Percent_Identity=29.8283261802575, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI237757300, Length=115, Percent_Identity=44.3478260869565, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI87082180, Length=457, Percent_Identity=35.4485776805252, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788468, Length=428, Percent_Identity=34.8130841121495, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788811, Length=456, Percent_Identity=35.5263157894737, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789606, Length=446, Percent_Identity=35.8744394618834, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789243, Length=435, Percent_Identity=28.735632183908, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=32.6710816777042, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=450, Percent_Identity=26.6666666666667, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320030, Length=290, Percent_Identity=28.6206896551724, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640763, Length=461, Percent_Identity=32.7548806941432, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI18858217, Length=461, Percent_Identity=32.7548806941432, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24665547, Length=469, Percent_Identity=28.7846481876333, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24665543, Length=469, Percent_Identity=28.7846481876333, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28574881, Length=469, Percent_Identity=28.7846481876333, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24665539, Length=469, Percent_Identity=28.7846481876333, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 46534; Mature: 46534

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQRTPGIVAERLSAEELARNFADLHAPLEPHEAAVAADRCYFCHDAPCVTACPTEIDI
CCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCCCCEEECCCCCCH
PLFIRQISTGTPEAAAKTIFDQNILGGMCARVCPTEDLCEQACVREMAEGKPVEIGRLQR
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YATDTLMQRDIHPYTRAAATGKRIAVVGAGPAGLSCAHRLALLGNDVVVYDGHAKPGGLN
HHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
EYGIAAYKSTDDFAAREVAWLLDIGGITVETGKVLGGDLALDALAAQYDAVFLAVGLGGV
CCCEEEECCCCHHHHHHEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCC
NALNLEGDDKSGVTDAVSFIETLRQSRDLTKLPVGRNVVVIGGGMTAVDAAVQSKLLGAE
EEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHHHCCCC
NVTIAYRRDRAAMSASRYEQDLAASHGVKLMFNVQPVAIHGNGAAAEIELEYTASVDGRL
CEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEEEEEECCCCEE
VGTGEKTRLTADQVFRAIGQTLTMEGGLETRNGKIAVSGAGRTSLDRVWAGGDCALGGDD
EECCCCCEECHHHHHHHHCCEEEECCCCEECCCEEEEECCCCCCHHHEECCCCEEECCCC
LTVTAVAEGRDAAMDIHAALNGSV
EEEEEEECCCCCCEEEEEECCCCC
>Mature Secondary Structure
MISQRTPGIVAERLSAEELARNFADLHAPLEPHEAAVAADRCYFCHDAPCVTACPTEIDI
CCCCCCCCHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCCEEEECCCCCEEECCCCCCH
PLFIRQISTGTPEAAAKTIFDQNILGGMCARVCPTEDLCEQACVREMAEGKPVEIGRLQR
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
YATDTLMQRDIHPYTRAAATGKRIAVVGAGPAGLSCAHRLALLGNDVVVYDGHAKPGGLN
HHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCC
EYGIAAYKSTDDFAAREVAWLLDIGGITVETGKVLGGDLALDALAAQYDAVFLAVGLGGV
CCCEEEECCCCHHHHHHEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCC
NALNLEGDDKSGVTDAVSFIETLRQSRDLTKLPVGRNVVVIGGGMTAVDAAVQSKLLGAE
EEEEECCCCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHHHHCCCC
NVTIAYRRDRAAMSASRYEQDLAASHGVKLMFNVQPVAIHGNGAAAEIELEYTASVDGRL
CEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEEEEEECCCCEE
VGTGEKTRLTADQVFRAIGQTLTMEGGLETRNGKIAVSGAGRTSLDRVWAGGDCALGGDD
EECCCCCEECHHHHHHHHCCEEEECCCCEECCCEEEEECCCCCCHHHEECCCCEEECCCC
LTVTAVAEGRDAAMDIHAALNGSV
EEEEEEECCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]