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The map label for this gene is tig

Identifier: 110680241

GI number: 110680241

Start: 2916749

End: 2918080

Strand: Reverse

Name: tig

Synonym: RD1_3049

Alternate gene names: 110680241

Gene position: 2918080-2916749 (Counterclockwise)

Preceding gene: 110680242

Following gene: 110680240

Centisome position: 70.6

GC content: 57.36

Gene sequence:

>1332_bases
ATGCAGGTCACCGAGACGCTGAATGAAGGTTTGAAACGCGGCTATGCCATCAAGGTCACCGCGGCTGAACTGGATGCCAA
GGTCAACGAGAAGCTGGCAGAAGCCCAGCCCGAAGTCGAAATGAAGGGCTTTCGCAAAGGCAAGGTGCCGATGGCGCTGC
TGAAAAAGCAGTTTGGCCAAAAGGTCATGGGCGAAGCAATGCAGGAAAGCATTGATGGTGCGATGAACGACCATTTCACG
TCGTCCGGGGATCGCCCTGCCATGCAGCCCGAGGTCAAGATGACCAACGAGAACTGGAAAGAGGGTGACGACATCGAGGT
GTCGATGTCCTACGAGGCGCTTCCCGATATCCCCGATGTTGATTTTTCAGCGATTGAGCTTGAGAGAATGACCGTCAAGG
CGGATGACGCCGCGATTGACGAAGCGCTGGGCAGTCTCGCAGAAACCGCACAGAATTTCGAGAACCGCAAGAAGGGTTCG
AAGGCGAAAGACGGCGACCAGATCGTCATGGACTTCCTCGGCAAGGTCGACGGTGAGGCCTTTGACGGCGGTGCGGCGGA
GGATTACCCGCTGGTGCTCGGGTCAAATTCCTTCATTCCGGGTTTCGAGGAGCAGCTTGTGGGCGTCAAGGCCGGGGAAG
AGAAAGCGGTCACCGTGACCTTCCCCGAGAATTACCAGGCGGAAAACCTTGCCGGCAAAGAAGCCGTGTTCGAATGCACT
GTCAAAGAGGTCAAGAAACCCGTTCCGGCGGAAATCGATGACGAGTTGGCCAAGAAGTTCGGCGCCGAAGACCTCGCGGC
CCTGAAGGGTCAGATCGCAGAGCGTCTGGAGGCGGAATATGCCGGTGCCGCACGCGCGATCATGAAGCGTTCCCTGCTGG
ATGCCCTTGATGACATCGTCGACTTTGACTTGCCGCCATCACTGGTCAGCGCAGAAGCCGGCCAAATTGCGCACCAACTC
TGGCATGAGGAAAACCCGGATGTGCAGGGACACGATCATCCGGAAATCACGCCGACCGAAGAACACACCAAGCTCGCGGA
GCGCCGCGTGCGTCTTGGGTTGTTGCTGGCCGATATCGGCCAGAAGGCCAAGGTCGAAGTGACGGATGCGGAGATGAGCC
AGGCGATCATGAACCAGGCGCGCCAGTACCCCGGTCAGGAGCGTCAGTTCTTTGAGTTCGTGCAGCAAAACCAGCAGATG
CAGCAGCAGATGCGTGCCCCGATCTTCGAAGACAAGGTCGTCGATCACGTGTTCGCAGGTGCAAAAGTCACCGAAAAGGA
AGTGTCCAAGGACGATCTGCAGAAAGCTGTCGAAGCGCTTGAAGAAGAGTAA

Upstream 100 bases:

>100_bases
ATCGGTGCGCAGCCTCTTGCACCTTGGCTGCGACGCACATAGAACAGCGGAAATTTTGATATCCGCGTCTGTCTGGCGCG
CCTTACCCTATGGAGCACAT

Downstream 100 bases:

>100_bases
AACACGCGATTTGCGTTGATTGATGAGACCGGTGTCAGAAATGACGCCGGTTTTTTCAATTTTACCGTCGTGATTTGAAA
TTCATTCAGTTTAAGGTTGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MQVTETLNEGLKRGYAIKVTAAELDAKVNEKLAEAQPEVEMKGFRKGKVPMALLKKQFGQKVMGEAMQESIDGAMNDHFT
SSGDRPAMQPEVKMTNENWKEGDDIEVSMSYEALPDIPDVDFSAIELERMTVKADDAAIDEALGSLAETAQNFENRKKGS
KAKDGDQIVMDFLGKVDGEAFDGGAAEDYPLVLGSNSFIPGFEEQLVGVKAGEEKAVTVTFPENYQAENLAGKEAVFECT
VKEVKKPVPAEIDDELAKKFGAEDLAALKGQIAERLEAEYAGAARAIMKRSLLDALDDIVDFDLPPSLVSAEAGQIAHQL
WHEENPDVQGHDHPEITPTEEHTKLAERRVRLGLLLADIGQKAKVEVTDAEMSQAIMNQARQYPGQERQFFEFVQQNQQM
QQQMRAPIFEDKVVDHVFAGAKVTEKEVSKDDLQKAVEALEEE

Sequences:

>Translated_443_residues
MQVTETLNEGLKRGYAIKVTAAELDAKVNEKLAEAQPEVEMKGFRKGKVPMALLKKQFGQKVMGEAMQESIDGAMNDHFT
SSGDRPAMQPEVKMTNENWKEGDDIEVSMSYEALPDIPDVDFSAIELERMTVKADDAAIDEALGSLAETAQNFENRKKGS
KAKDGDQIVMDFLGKVDGEAFDGGAAEDYPLVLGSNSFIPGFEEQLVGVKAGEEKAVTVTFPENYQAENLAGKEAVFECT
VKEVKKPVPAEIDDELAKKFGAEDLAALKGQIAERLEAEYAGAARAIMKRSLLDALDDIVDFDLPPSLVSAEAGQIAHQL
WHEENPDVQGHDHPEITPTEEHTKLAERRVRLGLLLADIGQKAKVEVTDAEMSQAIMNQARQYPGQERQFFEFVQQNQQM
QQQMRAPIFEDKVVDHVFAGAKVTEKEVSKDDLQKAVEALEEE
>Mature_443_residues
MQVTETLNEGLKRGYAIKVTAAELDAKVNEKLAEAQPEVEMKGFRKGKVPMALLKKQFGQKVMGEAMQESIDGAMNDHFT
SSGDRPAMQPEVKMTNENWKEGDDIEVSMSYEALPDIPDVDFSAIELERMTVKADDAAIDEALGSLAETAQNFENRKKGS
KAKDGDQIVMDFLGKVDGEAFDGGAAEDYPLVLGSNSFIPGFEEQLVGVKAGEEKAVTVTFPENYQAENLAGKEAVFECT
VKEVKKPVPAEIDDELAKKFGAEDLAALKGQIAERLEAEYAGAARAIMKRSLLDALDDIVDFDLPPSLVSAEAGQIAHQL
WHEENPDVQGHDHPEITPTEEHTKLAERRVRLGLLLADIGQKAKVEVTDAEMSQAIMNQARQYPGQERQFFEFVQQNQQM
QQQMRAPIFEDKVVDHVFAGAKVTEKEVSKDDLQKAVEALEEE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=441, Percent_Identity=31.7460317460317, Blast_Score=207, Evalue=9e-55,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_ROSDO (Q164N1)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683248.1
- ProteinModelPortal:   Q164N1
- SMR:   Q164N1
- STRING:   Q164N1
- GeneID:   4198752
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3049
- NMPDR:   fig|375451.6.peg.2835
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q164N1
- ProtClustDB:   PRK01490
- BioCyc:   RDEN375451:RD1_3049-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48903; Mature: 48903

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTETLNEGLKRGYAIKVTAAELDAKVNEKLAEAQPEVEMKGFRKGKVPMALLKKQFGQ
CCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHH
KVMGEAMQESIDGAMNDHFTSSGDRPAMQPEVKMTNENWKEGDDIEVSMSYEALPDIPDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCC
DFSAIELERMTVKADDAAIDEALGSLAETAQNFENRKKGSKAKDGDQIVMDFLGKVDGEA
CCHHEEHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
FDGGAAEDYPLVLGSNSFIPGFEEQLVGVKAGEEKAVTVTFPENYQAENLAGKEAVFECT
CCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHH
VKEVKKPVPAEIDDELAKKFGAEDLAALKGQIAERLEAEYAGAARAIMKRSLLDALDDIV
HHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFDLPPSLVSAEAGQIAHQLWHEENPDVQGHDHPEITPTEEHTKLAERRVRLGLLLADIG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QKAKVEVTDAEMSQAIMNQARQYPGQERQFFEFVQQNQQMQQQMRAPIFEDKVVDHVFAG
CCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
AKVTEKEVSKDDLQKAVEALEEE
CCCCHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQVTETLNEGLKRGYAIKVTAAELDAKVNEKLAEAQPEVEMKGFRKGKVPMALLKKQFGQ
CCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHH
KVMGEAMQESIDGAMNDHFTSSGDRPAMQPEVKMTNENWKEGDDIEVSMSYEALPDIPDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCC
DFSAIELERMTVKADDAAIDEALGSLAETAQNFENRKKGSKAKDGDQIVMDFLGKVDGEA
CCHHEEHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
FDGGAAEDYPLVLGSNSFIPGFEEQLVGVKAGEEKAVTVTFPENYQAENLAGKEAVFECT
CCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCHHHHHHHH
VKEVKKPVPAEIDDELAKKFGAEDLAALKGQIAERLEAEYAGAARAIMKRSLLDALDDIV
HHHHCCCCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFDLPPSLVSAEAGQIAHQLWHEENPDVQGHDHPEITPTEEHTKLAERRVRLGLLLADIG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
QKAKVEVTDAEMSQAIMNQARQYPGQERQFFEFVQQNQQMQQQMRAPIFEDKVVDHVFAG
CCCEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
AKVTEKEVSKDDLQKAVEALEEE
CCCCHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA