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The map label for this gene is pdhC [H]

Identifier: 110680209

GI number: 110680209

Start: 2889231

End: 2890526

Strand: Direct

Name: pdhC [H]

Synonym: RD1_3014

Alternate gene names: 110680209

Gene position: 2889231-2890526 (Clockwise)

Preceding gene: 110680208

Following gene: 110680233

Centisome position: 69.9

GC content: 61.65

Gene sequence:

>1296_bases
ATGCCTACAGAAATTCTCATGCCCGCCCTGAGCCCGACCATGGAAGAAGGCACACTGGCCAAATGGCTGGTGAAGGAGGG
GGACACCGTGGCCTCTGGCGATATTATGGCCGAGATCGAAACCGACAAGGCAACGATGGAATTCGAAGCCGTTGATGAAG
GCACCATCGGCAAGATCCTGGTCGAAGAAGGCACCGAAGGGGTGAAAGTGAACACTCCGATCGCGGTGCTTCTGGAAGAC
GGGGAAAGTGCGGATGACATCTCTGCCGAACCTGAGCCGGCTGCCGCAGCAACCAAAGAGGACGCACCCGCACCCACCCC
TGAACCAACAGCGACGCCCGCGCCGGCCGCGCCGCAGTCCTCGGATGGATCACGGATATTTGCATCCCCTCTTGCACGCA
GGATCGCGGCCAGCAATGGCGTTGATCTGGCCACGGTCAAAGGGTCCGGCCCGCATGGGCGGATCGTCAAGGCAGATGTC
GAGGGGCTCAGCGCAAGCGCTGCGGCCCCTGCCCCGGCTGCGCCCGGACCTGCGGCTCCAGCGCCGAGCGCGCCAGTTGC
ATCAGGTCCCGCCGCTGAAGCGGTGATGGCGATGTATGAAGGGCGCGCCTACGATGAAATCAGCCTCAACGGGATGCGCA
AGACGATCGCTGCGCGCCTGACCGAAGCCAAGCAGTCGATTCCCCATTTCTACCTGCGGCGCGACATCGAGCTTGATGCG
CTGTTGGCCTTCCGGGGGCAATTGAACAAGCAGCTGGAAAGCCGGGGCGTGAAGCTGTCGGTGAATGATTTCATCATCAA
GGCCTGTGCGCTGGCCCTGCAAACCGTGCCGGACGCGAACGCCGTTTGGGCCGGGGACCGCATGCTGAAACTCACACCGT
CTGACGTGGCTGTTGCCGTCGCGATTGAGGGTGGATTGTTCACGCCGGTTCTCAGGGACGCCGAGATGAAATCGCTCTCG
GCGCTCTCTGCCGAGATGAAGGACCTCGCGGCGCGCGCGCGCGACCGCAAGCTCGCCCCGCACGAATATCAGGGCGGTAG
CTTTGCGATTTCCAATCTTGGCATGTTCGGGATCGACAACTTCGATGCGGTCATCAACCCGCCCCACGGTGCTATTCTGG
CGGTTGGTGCAGGCGTGAAAAAACCTGTCGTCGGCAAGGACGGTGAATTGGCCGTTGCGACCGTGATGTCCGTCACTCTG
TCCGTAGACCACAGGGTGATTGATGGCGCTCTGGGCGCGCAACTCATATCGGCCATCAAGGAGAACCTTGAAAACCCGAT
GACAATGCTGGCGTAG

Upstream 100 bases:

>100_bases
CAATGCCCTATGCGGCCAATCTTGAAAAACACGCGCTGGTCACCACCGATGAAGTGATCGCTGCCGTGAAACAAGTCACC
TACAAGTAAGGAGCGCGCAG

Downstream 100 bases:

>100_bases
CACGGATAATCTGCGCGCATATCTGACGCATTCCATATCGCCAGACACGACATGGCCCGCGATTCTCAAAATCGCGGGCC
ATGTTGGTTAATACAGTATC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPTEILMPALSPTMEEGTLAKWLVKEGDTVASGDIMAEIETDKATMEFEAVDEGTIGKILVEEGTEGVKVNTPIAVLLED
GESADDISAEPEPAAAATKEDAPAPTPEPTATPAPAAPQSSDGSRIFASPLARRIAASNGVDLATVKGSGPHGRIVKADV
EGLSASAAAPAPAAPGPAAPAPSAPVASGPAAEAVMAMYEGRAYDEISLNGMRKTIAARLTEAKQSIPHFYLRRDIELDA
LLAFRGQLNKQLESRGVKLSVNDFIIKACALALQTVPDANAVWAGDRMLKLTPSDVAVAVAIEGGLFTPVLRDAEMKSLS
ALSAEMKDLAARARDRKLAPHEYQGGSFAISNLGMFGIDNFDAVINPPHGAILAVGAGVKKPVVGKDGELAVATVMSVTL
SVDHRVIDGALGAQLISAIKENLENPMTMLA

Sequences:

>Translated_431_residues
MPTEILMPALSPTMEEGTLAKWLVKEGDTVASGDIMAEIETDKATMEFEAVDEGTIGKILVEEGTEGVKVNTPIAVLLED
GESADDISAEPEPAAAATKEDAPAPTPEPTATPAPAAPQSSDGSRIFASPLARRIAASNGVDLATVKGSGPHGRIVKADV
EGLSASAAAPAPAAPGPAAPAPSAPVASGPAAEAVMAMYEGRAYDEISLNGMRKTIAARLTEAKQSIPHFYLRRDIELDA
LLAFRGQLNKQLESRGVKLSVNDFIIKACALALQTVPDANAVWAGDRMLKLTPSDVAVAVAIEGGLFTPVLRDAEMKSLS
ALSAEMKDLAARARDRKLAPHEYQGGSFAISNLGMFGIDNFDAVINPPHGAILAVGAGVKKPVVGKDGELAVATVMSVTL
SVDHRVIDGALGAQLISAIKENLENPMTMLA
>Mature_430_residues
PTEILMPALSPTMEEGTLAKWLVKEGDTVASGDIMAEIETDKATMEFEAVDEGTIGKILVEEGTEGVKVNTPIAVLLEDG
ESADDISAEPEPAAAATKEDAPAPTPEPTATPAPAAPQSSDGSRIFASPLARRIAASNGVDLATVKGSGPHGRIVKADVE
GLSASAAAPAPAAPGPAAPAPSAPVASGPAAEAVMAMYEGRAYDEISLNGMRKTIAARLTEAKQSIPHFYLRRDIELDAL
LAFRGQLNKQLESRGVKLSVNDFIIKACALALQTVPDANAVWAGDRMLKLTPSDVAVAVAIEGGLFTPVLRDAEMKSLSA
LSAEMKDLAARARDRKLAPHEYQGGSFAISNLGMFGIDNFDAVINPPHGAILAVGAGVKKPVVGKDGELAVATVMSVTLS
VDHRVIDGALGAQLISAIKENLENPMTMLA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=44.097995545657, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=38.562091503268, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=39.1684901531729, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=28.8018433179723, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=45, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI19923748, Length=233, Percent_Identity=32.618025751073, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786946, Length=428, Percent_Identity=32.0093457943925, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1786305, Length=421, Percent_Identity=33.729216152019, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=42.6048565121413, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=36.9774919614148, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=419, Percent_Identity=29.3556085918854, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=31.554524361949, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=40.1766004415011, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6320352, Length=439, Percent_Identity=28.0182232346241, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6321632, Length=173, Percent_Identity=32.3699421965318, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129315, Length=447, Percent_Identity=38.4787472035794, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24582497, Length=440, Percent_Identity=37.7272727272727, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI18859875, Length=422, Percent_Identity=30.5687203791469, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44606; Mature: 44475

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEILMPALSPTMEEGTLAKWLVKEGDTVASGDIMAEIETDKATMEFEAVDEGTIGKIL
CCCHHCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEECCCEEEEEEECCCCCCCEEE
VEEGTEGVKVNTPIAVLLEDGESADDISAEPEPAAAATKEDAPAPTPEPTATPAPAAPQS
EECCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDGSRIFASPLARRIAASNGVDLATVKGSGPHGRIVKADVEGLSASAAAPAPAAPGPAAP
CCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
APSAPVASGPAAEAVMAMYEGRAYDEISLNGMRKTIAARLTEAKQSIPHFYLRRDIELDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEHHCCCCHHH
LLAFRGQLNKQLESRGVKLSVNDFIIKACALALQTVPDANAVWAGDRMLKLTPSDVAVAV
HHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCEEEEE
AIEGGLFTPVLRDAEMKSLSALSAEMKDLAARARDRKLAPHEYQGGSFAISNLGMFGIDN
EECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEECCCC
FDAVINPPHGAILAVGAGVKKPVVGKDGELAVATVMSVTLSVDHRVIDGALGAQLISAIK
CCCEECCCCCEEEEECCCCCCCCCCCCCCEEHEEHHHHHHCCCHHHHHHHHHHHHHHHHH
ENLENPMTMLA
HHHCCCHHHCC
>Mature Secondary Structure 
PTEILMPALSPTMEEGTLAKWLVKEGDTVASGDIMAEIETDKATMEFEAVDEGTIGKIL
CCHHCCCCCCCCCCCCHHHHHHHHCCCEEECCCEEEEEECCCEEEEEEECCCCCCCEEE
VEEGTEGVKVNTPIAVLLEDGESADDISAEPEPAAAATKEDAPAPTPEPTATPAPAAPQS
EECCCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDGSRIFASPLARRIAASNGVDLATVKGSGPHGRIVKADVEGLSASAAAPAPAAPGPAAP
CCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
APSAPVASGPAAEAVMAMYEGRAYDEISLNGMRKTIAARLTEAKQSIPHFYLRRDIELDA
CCCCCCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEHHCCCCHHH
LLAFRGQLNKQLESRGVKLSVNDFIIKACALALQTVPDANAVWAGDRMLKLTPSDVAVAV
HHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCEEEEE
AIEGGLFTPVLRDAEMKSLSALSAEMKDLAARARDRKLAPHEYQGGSFAISNLGMFGIDN
EECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEECCCC
FDAVINPPHGAILAVGAGVKKPVVGKDGELAVATVMSVTLSVDHRVIDGALGAQLISAIK
CCCEECCCCCEEEEECCCCCCCCCCCCCCEEHEEHHHHHHCCCHHHHHHHHHHHHHHHHH
ENLENPMTMLA
HHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]