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The map label for this gene is pdhB [H]

Identifier: 110680208

GI number: 110680208

Start: 2887840

End: 2889219

Strand: Direct

Name: pdhB [H]

Synonym: RD1_3013

Alternate gene names: 110680208

Gene position: 2887840-2889219 (Clockwise)

Preceding gene: 110680207

Following gene: 110680209

Centisome position: 69.87

GC content: 57.1

Gene sequence:

>1380_bases
ATGGCTACTGAAATACTCATGCCCGCCCTATCGCCCACCATGGAAGAAGGCACACTCGCCAAATGGCTGGTCAAGGAAGG
AGACACGGTTTCCTCCGGTGACATCATGGCCGAGATTGAGACCGACAAGGCGACGATGGAATTCGAAGCCGTCGACGAAG
GTATCATCGGCAAGATCCTGATCGAAGAAGGCACCGAAGGCGTGAAAGTGAACACAGCCATCGCCATTCTTGTCGAGGAA
GGCGAAGATGTGCCTCAAGCCGGCGCTGACGCAGCAGAAGCCCCCATGCCTGCGGCACTGAAGGCTGAGGAAGGCAAGCC
ACCTGCCACGACACCAACCGCCGCCACGCCGGCAGCACCTGAAACGGATACAACGCCCGACTGGCCCGAAGGCACCCCCC
TGAAACAACAGACCGTGCGCGAAGCGCTGCGCGATGGCATGTCCGAAGAAATGCGCCGCGACGAAACGGTGTTCCTGATG
GGAGAAGAGGTCGCCGAATATCAGGGGGCCTACAAGATTTCCCAGGGGATGCTGGATGAATTCGGGTCCAAACGCGTGAT
TGATACGCCGATCACGGAACATGGCTTTGCCGGGATTGCGACCGGCGCCGCCTTTGGCGGTCTGCGCCCGATTGTGGAAT
TCATGACATTCAACTTCGCAATGCAGGCAATTGACCACATCATCAACTCCGCAGCCAAAACGCTTTACATGTCGGGGGGT
CAGATGGGCGCGCCAATGGTCTTCCGTGGGCCCAATGGTGCGGCGGCCCGTGTAGGGGCGCAACACAGTCAGGATTACGC
GGCATGGTACATGCAGGTGCCGGGTCTGAAGGTCGCGATGCCCTATGCTGCCTCTGATTACAAGGGGTTGATGAAAACTG
CGATCCGCGATCCGAACCCGGTAATTTTCCTTGAGAATGAAATCGTTTACGGGCGCACCTTTGATGTGCCTGACATCGAG
GATTACACCGTGCCCTTTGGCAAGGCCCGCATCTGGCGCGAAGGGTCCGATGTGACCATCGTCAGCTTTGGTATCGGCAT
GCAATATGCCCTTGAAGCCGCTGAAAAGCTTGCCGACGAAGGCATCTCGGCCGAGGTTGTCGATCTGCGTACCCTGCGCC
CCATGGATACGGCCAGCATCATCAAATCCGTGATGAAAACCAATCGTTGCGTGACCGTCGAAGAAGGATGGCCGCAAGGC
TCCGTAGGCAGCTACATCAGTTCGGTGATCATGCAGGAGGCATTTGACTATCTGGATGCGCCGGTCATCAATTGCACCGG
TAAAGACGTGCCAATGCCCTATGCGGCCAATCTTGAAAAACACGCGCTGGTCACCACCGATGAAGTGATCGCTGCCGTGA
AACAAGTCACCTACAAGTAA

Upstream 100 bases:

>100_bases
GAACGAGAGCGCGGAGTTTGCAAAAACATCCCCCGAGCCGGACCTGAAAGAGCTTTGGACCGACATCTACGCCACAGAAG
TTCCGCAGGAGGCTTGATCA

Downstream 100 bases:

>100_bases
GGAGCGCGCAGATGCCTACAGAAATTCTCATGCCCGCCCTGAGCCCGACCATGGAAGAAGGCACACTGGCCAAATGGCTG
GTGAAGGAGGGGGACACCGT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MATEILMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKATMEFEAVDEGIIGKILIEEGTEGVKVNTAIAILVEE
GEDVPQAGADAAEAPMPAALKAEEGKPPATTPTAATPAAPETDTTPDWPEGTPLKQQTVREALRDGMSEEMRRDETVFLM
GEEVAEYQGAYKISQGMLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSGG
QMGAPMVFRGPNGAAARVGAQHSQDYAAWYMQVPGLKVAMPYAASDYKGLMKTAIRDPNPVIFLENEIVYGRTFDVPDIE
DYTVPFGKARIWREGSDVTIVSFGIGMQYALEAAEKLADEGISAEVVDLRTLRPMDTASIIKSVMKTNRCVTVEEGWPQG
SVGSYISSVIMQEAFDYLDAPVINCTGKDVPMPYAANLEKHALVTTDEVIAAVKQVTYK

Sequences:

>Translated_459_residues
MATEILMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKATMEFEAVDEGIIGKILIEEGTEGVKVNTAIAILVEE
GEDVPQAGADAAEAPMPAALKAEEGKPPATTPTAATPAAPETDTTPDWPEGTPLKQQTVREALRDGMSEEMRRDETVFLM
GEEVAEYQGAYKISQGMLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSGG
QMGAPMVFRGPNGAAARVGAQHSQDYAAWYMQVPGLKVAMPYAASDYKGLMKTAIRDPNPVIFLENEIVYGRTFDVPDIE
DYTVPFGKARIWREGSDVTIVSFGIGMQYALEAAEKLADEGISAEVVDLRTLRPMDTASIIKSVMKTNRCVTVEEGWPQG
SVGSYISSVIMQEAFDYLDAPVINCTGKDVPMPYAANLEKHALVTTDEVIAAVKQVTYK
>Mature_458_residues
ATEILMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKATMEFEAVDEGIIGKILIEEGTEGVKVNTAIAILVEEG
EDVPQAGADAAEAPMPAALKAEEGKPPATTPTAATPAAPETDTTPDWPEGTPLKQQTVREALRDGMSEEMRRDETVFLMG
EEVAEYQGAYKISQGMLDEFGSKRVIDTPITEHGFAGIATGAAFGGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSGGQ
MGAPMVFRGPNGAAARVGAQHSQDYAAWYMQVPGLKVAMPYAASDYKGLMKTAIRDPNPVIFLENEIVYGRTFDVPDIED
YTVPFGKARIWREGSDVTIVSFGIGMQYALEAAEKLADEGISAEVVDLRTLRPMDTASIIKSVMKTNRCVTVEEGWPQGS
VGSYISSVIMQEAFDYLDAPVINCTGKDVPMPYAANLEKHALVTTDEVIAAVKQVTYK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=57.4074074074074, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=54.0123456790123, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI4557353, Length=302, Percent_Identity=34.1059602649007, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI34101272, Length=302, Percent_Identity=34.1059602649007, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI203098753, Length=80, Percent_Identity=56.25, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI203098816, Length=80, Percent_Identity=56.25, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI31711992, Length=133, Percent_Identity=37.593984962406, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=57, Percent_Identity=61.4035087719298, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI133778974, Length=159, Percent_Identity=30.188679245283, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786622, Length=333, Percent_Identity=23.7237237237237, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=61.4906832298137, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=36.5325077399381, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=142, Percent_Identity=40.1408450704225, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=58.4097859327217, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324258, Length=127, Percent_Identity=45.6692913385827, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321632, Length=110, Percent_Identity=39.0909090909091, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=58.2822085889571, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=58.2822085889571, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI160714832, Length=302, Percent_Identity=34.7682119205298, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI160714828, Length=302, Percent_Identity=34.7682119205298, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24650943, Length=92, Percent_Identity=65.2173913043478, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24650945, Length=92, Percent_Identity=65.2173913043478, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129315, Length=88, Percent_Identity=50, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582497, Length=81, Percent_Identity=46.9135802469136, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 49479; Mature: 49348

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEILMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKATMEFEAVDEGIIGKIL
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCEEEEE
IEEGTEGVKVNTAIAILVEEGEDVPQAGADAAEAPMPAALKAEEGKPPATTPTAATPAAP
EECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
ETDTTPDWPEGTPLKQQTVREALRDGMSEEMRRDETVFLMGEEVAEYQGAYKISQGMLDE
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHH
FGSKRVIDTPITEHGFAGIATGAAFGGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSGG
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
QMGAPMVFRGPNGAAARVGAQHSQDYAAWYMQVPGLKVAMPYAASDYKGLMKTAIRDPNP
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCC
VIFLENEIVYGRTFDVPDIEDYTVPFGKARIWREGSDVTIVSFGIGMQYALEAAEKLADE
EEEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHC
GISAEVVDLRTLRPMDTASIIKSVMKTNRCVTVEEGWPQGSVGSYISSVIMQEAFDYLDA
CCCEEEEEHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
PVINCTGKDVPMPYAANLEKHALVTTDEVIAAVKQVTYK
CEEECCCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATEILMPALSPTMEEGTLAKWLVKEGDTVSSGDIMAEIETDKATMEFEAVDEGIIGKIL
CCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHCCCCEEEEE
IEEGTEGVKVNTAIAILVEEGEDVPQAGADAAEAPMPAALKAEEGKPPATTPTAATPAAP
EECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCC
ETDTTPDWPEGTPLKQQTVREALRDGMSEEMRRDETVFLMGEEVAEYQGAYKISQGMLDE
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHHHH
FGSKRVIDTPITEHGFAGIATGAAFGGLRPIVEFMTFNFAMQAIDHIINSAAKTLYMSGG
HCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
QMGAPMVFRGPNGAAARVGAQHSQDYAAWYMQVPGLKVAMPYAASDYKGLMKTAIRDPNP
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCC
VIFLENEIVYGRTFDVPDIEDYTVPFGKARIWREGSDVTIVSFGIGMQYALEAAEKLADE
EEEEECEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHC
GISAEVVDLRTLRPMDTASIIKSVMKTNRCVTVEEGWPQGSVGSYISSVIMQEAFDYLDA
CCCEEEEEHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
PVINCTGKDVPMPYAANLEKHALVTTDEVIAAVKQVTYK
CEEECCCCCCCCCHHCCCCCCEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]