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The map label for this gene is pgk

Identifier: 110680205

GI number: 110680205

Start: 2884976

End: 2886163

Strand: Direct

Name: pgk

Synonym: RD1_3008

Alternate gene names: 110680205

Gene position: 2884976-2886163 (Clockwise)

Preceding gene: 110680201

Following gene: 110680206

Centisome position: 69.8

GC content: 62.63

Gene sequence:

>1188_bases
ATGAGTTGGAAGACATTGGATGATATGGATGTGGCGGGCAAGCGGGTGCTGCTGCGTGTGGATATCAACGTGCCGGTCGA
GGAGGGTCAGGTCACGGACCGAACGCGGATTGATCGCATCATTCCGACGCTGCAGGATATTCTGGCCAAGGGCGGCACGC
CGATCATGCTGGCGCATTTCGGGCGTCCCAAAGGCAAGGTTGTGCCGGAAATGTCGCTGCGCGTGACCCTGCCCGCGCTT
GAGGCCGCTTTGGGGCGCAAGGTCACATTCATCGAAGAGCCCAACGCGGAGCGCCTGTCTGATTTGCCGGAAGGCATCGT
TGTTCTGCTGGAAAACACGCGCTTTGCAGTGGGCGAAGAGGCCAATGACCCGGAAATGGCGCGGTTTCTGGCCACGCTCG
GCGATGTCTTTTGCAATGACGCCTTTTCAGCCGCCCACCGTGCGCATGCCTCCACCACGGGGGTTGCGCATCTGTTGCCG
TCCTGCGCGGGCCGCTTGATGCAGGCGGAGCTTTCGGCCTTGGAAGCGGCCCTGTCGACGCCACAACGCCCCGTCGGCGC
GGTGGTGGGCGGGGCCAAGGTTTCGACGAAAATCGCACTGCTTGAAAATCTGGTTGCCCGCCTCGATGTGCTCGTGATCG
GGGGCGGCATGGCCAACACTTTCCTCGCCGCACAGGGCGCGAAACTGGGCGCGTCGCTGATGGAGCCGGATTACATGGAA
ACCGCTCGGACGATCATGCAGTCTGCCGAGCGTGCGGGGTGCAAGCTGCTGCTGCCGGTCGACGGGGTCGTCGCGCGCGA
ATTCAAGGCGGGTGCAGACTTTGAGGTTGTCGCCCTGGGTAAGGACACGGTTCTGGCCGAGGACCAGATGGTTCTGGATG
CCGGCCCCCAAAGCGTGGCGGCCGTCATGGCTGCGTTCAGAACGCTCAAGACACTGATCTGGAACGGCCCGCTGGGCGCA
TTTGAAATTACCCCCTTCAATCAGGCCACAAATGCCACCGCTTTGGAGGCGGCGCGGCTGACCAAATCAGGCGCGCTGAT
CTCAGTTGCGGGCGGCGGTGATACGGTTGCCGCACTCAATCAGGCGGGTGCCGCCGCGGATTTCACGTATATTTCAACCG
CCGGTGGCGCGTTTCTGGAATGGATGGAGGGCAAAACCCTTCCCGGGGTCGCGGCACTGCAAGGCTGA

Upstream 100 bases:

>100_bases
TTACCTACGCGCTGCGATCAGGAATGCCAAGCCGCTCGGTTGACCTCAGCGACGATAAGCGCAAAACACGGACGCAGGCG
AGGCAGACGGAGGGTCCGGC

Downstream 100 bases:

>100_bases
AGCGTTATTCGAAAACCCTAAACCATATACCGCTGCCTGAAATCATCGATTTCAACGCTTAACATGAGATGCGATGCCTC
AAGTCGCTCACAAAACGCCA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSWKTLDDMDVAGKRVLLRVDINVPVEEGQVTDRTRIDRIIPTLQDILAKGGTPIMLAHFGRPKGKVVPEMSLRVTLPAL
EAALGRKVTFIEEPNAERLSDLPEGIVVLLENTRFAVGEEANDPEMARFLATLGDVFCNDAFSAAHRAHASTTGVAHLLP
SCAGRLMQAELSALEAALSTPQRPVGAVVGGAKVSTKIALLENLVARLDVLVIGGGMANTFLAAQGAKLGASLMEPDYME
TARTIMQSAERAGCKLLLPVDGVVAREFKAGADFEVVALGKDTVLAEDQMVLDAGPQSVAAVMAAFRTLKTLIWNGPLGA
FEITPFNQATNATALEAARLTKSGALISVAGGGDTVAALNQAGAAADFTYISTAGGAFLEWMEGKTLPGVAALQG

Sequences:

>Translated_395_residues
MSWKTLDDMDVAGKRVLLRVDINVPVEEGQVTDRTRIDRIIPTLQDILAKGGTPIMLAHFGRPKGKVVPEMSLRVTLPAL
EAALGRKVTFIEEPNAERLSDLPEGIVVLLENTRFAVGEEANDPEMARFLATLGDVFCNDAFSAAHRAHASTTGVAHLLP
SCAGRLMQAELSALEAALSTPQRPVGAVVGGAKVSTKIALLENLVARLDVLVIGGGMANTFLAAQGAKLGASLMEPDYME
TARTIMQSAERAGCKLLLPVDGVVAREFKAGADFEVVALGKDTVLAEDQMVLDAGPQSVAAVMAAFRTLKTLIWNGPLGA
FEITPFNQATNATALEAARLTKSGALISVAGGGDTVAALNQAGAAADFTYISTAGGAFLEWMEGKTLPGVAALQG
>Mature_394_residues
SWKTLDDMDVAGKRVLLRVDINVPVEEGQVTDRTRIDRIIPTLQDILAKGGTPIMLAHFGRPKGKVVPEMSLRVTLPALE
AALGRKVTFIEEPNAERLSDLPEGIVVLLENTRFAVGEEANDPEMARFLATLGDVFCNDAFSAAHRAHASTTGVAHLLPS
CAGRLMQAELSALEAALSTPQRPVGAVVGGAKVSTKIALLENLVARLDVLVIGGGMANTFLAAQGAKLGASLMEPDYMET
ARTIMQSAERAGCKLLLPVDGVVAREFKAGADFEVVALGKDTVLAEDQMVLDAGPQSVAAVMAAFRTLKTLIWNGPLGAF
EITPFNQATNATALEAARLTKSGALISVAGGGDTVAALNQAGAAADFTYISTAGGAFLEWMEGKTLPGVAALQG

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=38.7951807228916, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=38.6473429951691, Blast_Score=255, Evalue=5e-68,
Organism=Escherichia coli, GI1789294, Length=398, Percent_Identity=40.9547738693467, Blast_Score=277, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17508823, Length=409, Percent_Identity=38.3863080684597, Blast_Score=271, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=36.919315403423, Blast_Score=238, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=37.2881355932203, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=37.0731707317073, Blast_Score=237, Evalue=9e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ROSDO (Q164R7)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_683212.1
- ProteinModelPortal:   Q164R7
- SMR:   Q164R7
- STRING:   Q164R7
- GeneID:   4195226
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_3008
- NMPDR:   fig|375451.6.peg.2798
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   RITEFLP
- ProtClustDB:   PRK00073
- BioCyc:   RDEN375451:RD1_3008-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41343; Mature: 41212

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 114-114 BINDING 147-147 BINDING 197-197 BINDING 322-322

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWKTLDDMDVAGKRVLLRVDINVPVEEGQVTDRTRIDRIIPTLQDILAKGGTPIMLAHF
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGKVVPEMSLRVTLPALEAALGRKVTFIEEPNAERLSDLPEGIVVLLENTRFAVGEE
CCCCCCCCCCCEEEEEHHHHHHHHCCEEEEEECCCHHHHHCCCCEEEEEEECCEEEECCC
ANDPEMARFLATLGDVFCNDAFSAAHRAHASTTGVAHLLPSCAGRLMQAELSALEAALST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQRPVGAVVGGAKVSTKIALLENLVARLDVLVIGGGMANTFLAAQGAKLGASLMEPDYME
CCCCHHHEECCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCHHCHHHCCCHHHH
TARTIMQSAERAGCKLLLPVDGVVAREFKAGADFEVVALGKDTVLAEDQMVLDAGPQSVA
HHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCEEEEEECCCCEEECCCEEECCCHHHHH
AVMAAFRTLKTLIWNGPLGAFEITPFNQATNATALEAARLTKSGALISVAGGGDTVAALN
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHH
QAGAAADFTYISTAGGAFLEWMEGKTLPGVAALQG
HCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SWKTLDDMDVAGKRVLLRVDINVPVEEGQVTDRTRIDRIIPTLQDILAKGGTPIMLAHF
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGKVVPEMSLRVTLPALEAALGRKVTFIEEPNAERLSDLPEGIVVLLENTRFAVGEE
CCCCCCCCCCCEEEEEHHHHHHHHCCEEEEEECCCHHHHHCCCCEEEEEEECCEEEECCC
ANDPEMARFLATLGDVFCNDAFSAAHRAHASTTGVAHLLPSCAGRLMQAELSALEAALST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQRPVGAVVGGAKVSTKIALLENLVARLDVLVIGGGMANTFLAAQGAKLGASLMEPDYME
CCCCHHHEECCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHCCCHHCHHHCCCHHHH
TARTIMQSAERAGCKLLLPVDGVVAREFKAGADFEVVALGKDTVLAEDQMVLDAGPQSVA
HHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCEEEEEECCCCEEECCCEEECCCHHHHH
AVMAAFRTLKTLIWNGPLGAFEITPFNQATNATALEAARLTKSGALISVAGGGDTVAALN
HHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHH
QAGAAADFTYISTAGGAFLEWMEGKTLPGVAALQG
HCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA