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The map label for this gene is rafA [H]

Identifier: 110680070

GI number: 110680070

Start: 2734352

End: 2736436

Strand: Direct

Name: rafA [H]

Synonym: RD1_2865

Alternate gene names: 110680070

Gene position: 2734352-2736436 (Clockwise)

Preceding gene: 110680069

Following gene: 110680071

Centisome position: 66.16

GC content: 64.7

Gene sequence:

>2085_bases
GTGACAGAGTGCTGGCGGCTGGATGACGCGCGCCAGACGCTGGCGCTTGCCTCGACAAATGGGCGGTGCCCGCAGGTCAT
TTACTGGGGCGCGCCCCTGCCACCGGGCGAAGACCTGTGCGCGCTCAGCGATGTCAGCACGCTCGATGTGACGGGTGGCA
TGCTGGATCAGAACCCGGATCTGTCGCTCTGCCCTGAGGCTGCACGCAGCTTCCCGGGCCAGCCCGGTCTCATTGTGCGT
GACCGGGACGGCACGCCTTTCCTGCCGAAATTCCGGCTGGATCACGTGACACAGTCCGAGCTGACCTTGACCGTTACCTG
CGTCGATGCGCCCCACGGGCTGACCTATGTGGCCGAATTCACGCTGGATGCCGACACCCATGTGATTGCCATGACAGCAC
GGTTATCTGCTGACCGCCCGCTGCATCTGGACTGGCTGGCGGCCCCCGTCCTGCCCGCCCCGCAGCACAGCGAGGCGATG
ATTGATTTCGCAGGCCGCTGGTGTGGTGAGTTTCAGATGAACCGCACGCCATGGTCGCCGGGCATCCGCATGCGCGAAAA
CCGCACGGGGCGCACGGGCCACGAACATTTCCCCGGTCTGATCGTGCCCTGCAAGGGGGCGCGCAATACCGCAGGCGAGG
CCTATGCCTTTCACTATGGCTGGTCCGGCGGGCATCGGATGATCGCCGAAGAACTGCCCGATGGGCGCCGTCAAATTCAA
TTCGGGCACGCCGCAGGCACGCACATGACGCCAGCCACCGAGTTTCAGACCGCGCCTATCTATGCGGTCTATTCCTGCGA
GGGCCTGAACGGCTGCGCCGTCCCGTTTCAACGCCATGTGCGCGACCGGATCGTCGCGTGGACCGACACGGGCCGTCCGC
GCCCGGTGCATTACAACTGCTGGGAAGCGGTCTATTTCAACCACGACCTCGCCGTGCTCAAGGATATCGCCAGCCGCGCC
GCCGCCCTTGGTGCGGAGCGTTTCGTGCTGGATGACGGATGGTTCGGGCAGCGCGACGATGACACCTCTTCGCTGGGCGA
TTGGGCGGTGGATACACGCAAATACCCCGAAGGCCTCGACCCGCTGATCGCGCATGTGCGGGCACAGGGCATGGGTTTCG
GCATCTGGTTTGAACCGGAAATGATCAACCCGGACTCAGACACCTACCGCGCACATCCCGACTGGGCGCTGGGGCCTGCG
GACCAGATCCTTGGACGCCAGCAAAAACTGCTGAACATGGCCCTGCCACAGGTGCGCGATTATCTCTATGACCGCATCGC
CGCGCTGCTGACCGCCCATCCGATCGACTACATCAAATGGGACCACAACCGCGTCCTGCCCATGCCCGATGCGGCGCAGA
CCGACGGGAGCTATGCGTTGATCGACCGGCTGCGCGCCGATTTCCCGCAGGTGGAAATCGAAAGCTGCGCCTCGGGCGGT
GGGCGGATTGATTTCGGCATCCTGCAGCGCAGCCAAAGGGTCTGGTTGTCTGACAGCAACGACGCGTTGGAACGCCTCAG
AATTCAGCACAACGCCGCGCTGTTCCTGCCCGCCTGCGTCACGGGCAGCCATGTGGGCCCGCGCAGATGCCATACCTCGG
GCCGTGTCCTCGACATTGCATTGCGCGCATGGGTCGCCGCACAGCGCCACATGGGTTTCGAGATGGACCCGCGCGAATTG
ACCGAGCGCGAGGCGGAAGTGCTGCGCGATGTCACCGGGTGGTGGAAAGACAACCGCGACTGGATGGAACACGCGGACAT
CCTGCGGCTGGACAGTTCGGACCCTGCGGTCATCGCAGAACAACAACTGGCGCGCGACGGGCGCCAGTTCGTAGCCTTTG
TGGGCAAATCCGGCACCTCGGATCAGATCGCCCCACGTCCCTTGCGCCTTACGCGGCTGGACCCCGATGCGCGCTACGCG
GTCAGCCTGCGCAACCGCGCCGATGCCCCGCATCTGTCGCGCGGGTCTTTGGCGCTCAAGGACGGCCCTGTGACACTATC
CGGTGCGGCGCTGATGCAGCATGGCGTGACACTGCCATGGAGCTTTCCCGATTTCATCTGGGTGCTGGAGGGCCGCCGCC
TGTGA

Upstream 100 bases:

>100_bases
TGATGAGCGCGGGCAGCATTGTCGCCGCCCTGCCCCCGGTTCTGATGTTCTTTCTGATGCAAAAGCACTTTATCGCAGGT
TTGACGCTGGGCGCCGTCAA

Downstream 100 bases:

>100_bases
CCAAATTCGCCAAAACCAAACTGGCCCTGCGGCGTCTGCCCATGTACCCCTTGCACGGGAGGACGCAGCCCCTGACACCC
CCAACTTCGGAGAGACCCCG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTECWRLDDARQTLALASTNGRCPQVIYWGAPLPPGEDLCALSDVSTLDVTGGMLDQNPDLSLCPEAARSFPGQPGLIVR
DRDGTPFLPKFRLDHVTQSELTLTVTCVDAPHGLTYVAEFTLDADTHVIAMTARLSADRPLHLDWLAAPVLPAPQHSEAM
IDFAGRWCGEFQMNRTPWSPGIRMRENRTGRTGHEHFPGLIVPCKGARNTAGEAYAFHYGWSGGHRMIAEELPDGRRQIQ
FGHAAGTHMTPATEFQTAPIYAVYSCEGLNGCAVPFQRHVRDRIVAWTDTGRPRPVHYNCWEAVYFNHDLAVLKDIASRA
AALGAERFVLDDGWFGQRDDDTSSLGDWAVDTRKYPEGLDPLIAHVRAQGMGFGIWFEPEMINPDSDTYRAHPDWALGPA
DQILGRQQKLLNMALPQVRDYLYDRIAALLTAHPIDYIKWDHNRVLPMPDAAQTDGSYALIDRLRADFPQVEIESCASGG
GRIDFGILQRSQRVWLSDSNDALERLRIQHNAALFLPACVTGSHVGPRRCHTSGRVLDIALRAWVAAQRHMGFEMDPREL
TEREAEVLRDVTGWWKDNRDWMEHADILRLDSSDPAVIAEQQLARDGRQFVAFVGKSGTSDQIAPRPLRLTRLDPDARYA
VSLRNRADAPHLSRGSLALKDGPVTLSGAALMQHGVTLPWSFPDFIWVLEGRRL

Sequences:

>Translated_694_residues
MTECWRLDDARQTLALASTNGRCPQVIYWGAPLPPGEDLCALSDVSTLDVTGGMLDQNPDLSLCPEAARSFPGQPGLIVR
DRDGTPFLPKFRLDHVTQSELTLTVTCVDAPHGLTYVAEFTLDADTHVIAMTARLSADRPLHLDWLAAPVLPAPQHSEAM
IDFAGRWCGEFQMNRTPWSPGIRMRENRTGRTGHEHFPGLIVPCKGARNTAGEAYAFHYGWSGGHRMIAEELPDGRRQIQ
FGHAAGTHMTPATEFQTAPIYAVYSCEGLNGCAVPFQRHVRDRIVAWTDTGRPRPVHYNCWEAVYFNHDLAVLKDIASRA
AALGAERFVLDDGWFGQRDDDTSSLGDWAVDTRKYPEGLDPLIAHVRAQGMGFGIWFEPEMINPDSDTYRAHPDWALGPA
DQILGRQQKLLNMALPQVRDYLYDRIAALLTAHPIDYIKWDHNRVLPMPDAAQTDGSYALIDRLRADFPQVEIESCASGG
GRIDFGILQRSQRVWLSDSNDALERLRIQHNAALFLPACVTGSHVGPRRCHTSGRVLDIALRAWVAAQRHMGFEMDPREL
TEREAEVLRDVTGWWKDNRDWMEHADILRLDSSDPAVIAEQQLARDGRQFVAFVGKSGTSDQIAPRPLRLTRLDPDARYA
VSLRNRADAPHLSRGSLALKDGPVTLSGAALMQHGVTLPWSFPDFIWVLEGRRL
>Mature_693_residues
TECWRLDDARQTLALASTNGRCPQVIYWGAPLPPGEDLCALSDVSTLDVTGGMLDQNPDLSLCPEAARSFPGQPGLIVRD
RDGTPFLPKFRLDHVTQSELTLTVTCVDAPHGLTYVAEFTLDADTHVIAMTARLSADRPLHLDWLAAPVLPAPQHSEAMI
DFAGRWCGEFQMNRTPWSPGIRMRENRTGRTGHEHFPGLIVPCKGARNTAGEAYAFHYGWSGGHRMIAEELPDGRRQIQF
GHAAGTHMTPATEFQTAPIYAVYSCEGLNGCAVPFQRHVRDRIVAWTDTGRPRPVHYNCWEAVYFNHDLAVLKDIASRAA
ALGAERFVLDDGWFGQRDDDTSSLGDWAVDTRKYPEGLDPLIAHVRAQGMGFGIWFEPEMINPDSDTYRAHPDWALGPAD
QILGRQQKLLNMALPQVRDYLYDRIAALLTAHPIDYIKWDHNRVLPMPDAAQTDGSYALIDRLRADFPQVEIESCASGGG
RIDFGILQRSQRVWLSDSNDALERLRIQHNAALFLPACVTGSHVGPRRCHTSGRVLDIALRAWVAAQRHMGFEMDPRELT
EREAEVLRDVTGWWKDNRDWMEHADILRLDSSDPAVIAEQQLARDGRQFVAFVGKSGTSDQIAPRPLRLTRLDPDARYAV
SLRNRADAPHLSRGSLALKDGPVTLSGAALMQHGVTLPWSFPDFIWVLEGRRL

Specific function: Unknown

COG id: COG3345

COG function: function code G; Alpha-galactosidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 36 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002252
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 77454; Mature: 77323

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTECWRLDDARQTLALASTNGRCPQVIYWGAPLPPGEDLCALSDVSTLDVTGGMLDQNPD
CCCCCCCCCHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCCC
LSLCPEAARSFPGQPGLIVRDRDGTPFLPKFRLDHVTQSELTLTVTCVDAPHGLTYVAEF
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCEEEEEE
TLDADTHVIAMTARLSADRPLHLDWLAAPVLPAPQHSEAMIDFAGRWCGEFQMNRTPWSP
EECCCCEEEEEEEECCCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GIRMRENRTGRTGHEHFPGLIVPCKGARNTAGEAYAFHYGWSGGHRMIAEELPDGRRQIQ
CCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCHHEEE
FGHAAGTHMTPATEFQTAPIYAVYSCEGLNGCAVPFQRHVRDRIVAWTDTGRPRPVHYNC
ECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCEEEEECCCCCCCEEECC
WEAVYFNHDLAVLKDIASRAAALGAERFVLDDGWFGQRDDDTSSLGDWAVDTRKYPEGLD
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLIAHVRAQGMGFGIWFEPEMINPDSDTYRAHPDWALGPADQILGRQQKLLNMALPQVRD
HHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLYDRIAALLTAHPIDYIKWDHNRVLPMPDAAQTDGSYALIDRLRADFPQVEIESCASGG
HHHHHHHHHHHHCCCHHEEECCCEECCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHCCCC
GRIDFGILQRSQRVWLSDSNDALERLRIQHNAALFLPACVTGSHVGPRRCHTSGRVLDIA
CEEEHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCEEHHH
LRAWVAAQRHMGFEMDPRELTEREAEVLRDVTGWWKDNRDWMEHADILRLDSSDPAVIAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCEEHH
QQLARDGRQFVAFVGKSGTSDQIAPRPLRLTRLDPDARYAVSLRNRADAPHLSRGSLALK
HHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEE
DGPVTLSGAALMQHGVTLPWSFPDFIWVLEGRRL
CCCEEEECHHHHHCCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
TECWRLDDARQTLALASTNGRCPQVIYWGAPLPPGEDLCALSDVSTLDVTGGMLDQNPD
CCCCCCCCHHHEEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCCC
LSLCPEAARSFPGQPGLIVRDRDGTPFLPKFRLDHVTQSELTLTVTCVDAPHGLTYVAEF
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHCCCCCCCEEEEEEEECCCCCCEEEEEE
TLDADTHVIAMTARLSADRPLHLDWLAAPVLPAPQHSEAMIDFAGRWCGEFQMNRTPWSP
EECCCCEEEEEEEECCCCCCEEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GIRMRENRTGRTGHEHFPGLIVPCKGARNTAGEAYAFHYGWSGGHRMIAEELPDGRRQIQ
CCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCHHEEE
FGHAAGTHMTPATEFQTAPIYAVYSCEGLNGCAVPFQRHVRDRIVAWTDTGRPRPVHYNC
ECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCEEEEECCCCCCCEEECC
WEAVYFNHDLAVLKDIASRAAALGAERFVLDDGWFGQRDDDTSSLGDWAVDTRKYPEGLD
EEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHH
PLIAHVRAQGMGFGIWFEPEMINPDSDTYRAHPDWALGPADQILGRQQKLLNMALPQVRD
HHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
YLYDRIAALLTAHPIDYIKWDHNRVLPMPDAAQTDGSYALIDRLRADFPQVEIESCASGG
HHHHHHHHHHHHCCCHHEEECCCEECCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHCCCC
GRIDFGILQRSQRVWLSDSNDALERLRIQHNAALFLPACVTGSHVGPRRCHTSGRVLDIA
CEEEHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCEEHHH
LRAWVAAQRHMGFEMDPRELTEREAEVLRDVTGWWKDNRDWMEHADILRLDSSDPAVIAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCEEHH
QQLARDGRQFVAFVGKSGTSDQIAPRPLRLTRLDPDARYAVSLRNRADAPHLSRGSLALK
HHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEE
DGPVTLSGAALMQHGVTLPWSFPDFIWVLEGRRL
CCCEEEECHHHHHCCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2556373; 2180920; 786627 [H]