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The map label for this gene is ispG [H]

Identifier: 110680032

GI number: 110680032

Start: 2695792

End: 2696952

Strand: Reverse

Name: ispG [H]

Synonym: RD1_2825

Alternate gene names: 110680032

Gene position: 2696952-2695792 (Counterclockwise)

Preceding gene: 110680033

Following gene: 110680029

Centisome position: 65.25

GC content: 55.99

Gene sequence:

>1161_bases
GTGACAGGCACAGTAATAAAGGTCCCTTCGATGTCGCTAAATCATGTACGCCCCTGGCGCAACATATACCGCAGAGCATC
ACGCCAGATCATGGTCGGCAACGTGCCGGTCGGGGGCGACGCGCCGATCACTGTGCAGACGATGACCAACACGCTGACCA
CCGATGTCAAAGCCACGATTGATCAAATCAACGCGGCAACCGACGCCGGGGCGGATATCGTTCGTGTCTCCGTTCCTGAT
GAAGCCTCTTCCAAGGCGCTGAAACAGATCGTGCCAGAAGTTTCGGTGCCGATCGTGGCGGACATTCATTTTCATTACAA
AAGAGGGATCGAAGCGGCTGAGGCGGGGGCGGCATGCCTGCGGATTAACCCTGGCAACATCGGCGACGAAAAACGTGTAA
AAGAAGTGATCAAGGCCGCGCGCGATCATAATTGTTCGATCCGGATCGGCGTGAATGCGGGCAGCCTCGAAAAACATCTT
TTGGATAAATACGGTGAACCCTGTCCTGATGCCATGGTGGAAAGTGGGCTCGACCACATCAAAATCCTGCAGGACAATGA
TTTTCACGAATTCAAGATCAGCTGCAAGGCCTCCGATGTATTCATGGCGGCGGCGGCCTATCAGCAACTGGCTGATGCAA
CAGATGCCCCCATTCATCTTGGCATTACCGAAGCGGGCGGCCTCGTGTCTGGAACGATCAAATCCGCGATCGGGCTGGGT
AATCTGCTGTGGATGGGTATTGGCGATACCATCCGTGTCAGCCTGTCGGCGGACCCTGTCGAAGAGGTTAAGGTTGGTTA
CGAAATCCTGAAATCACTGGGCTTGCGTCATCGCGGTGTGAACATCATCAGCTGCCCCAGTTGCGCGCGGCAGGGTTTTG
ACGTGATCAAAACGGTTGAGACACTCGAAAAACGTCTGGAGCATATCAGAACGCCGATGAGCCTGAGCATCATCGGCTGT
GTGGTTAACGGCCCGGGCGAGGCGCTGATGACCGATGTCGGCTTTACGGGCGGCGGTGCCGGGTCGGGGATGGTCTATCT
GGCAGGCAGCCAGAGCCACAAACTCAACAATGACCAGATGATCGAGCATATCGTGGAGCAGGTCGAAAAAAAGGCCGCCG
AGCTTGAGGCTGCGGCAGCCCATGCGGATGCGGCCGAATAA

Upstream 100 bases:

>100_bases
CCGTGGTTGAACTGCAGACCCCTATCGAACCTGCGTCAGAATAACAGCACATGAAATTGAAAACGGTGATTGCGGGTTCG
GGCCTGAGTGTCTAATTTAA

Downstream 100 bases:

>100_bases
CGCGGTCGATCAACCGCGTTTTTCTTCAACGATCATTTGCAGCTGGTCCATCGGCAGGAACCCACGCAACATCTCATCTT
CGAGCACGAAAGTCGGTGTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTGTVIKVPSMSLNHVRPWRNIYRRASRQIMVGNVPVGGDAPITVQTMTNTLTTDVKATIDQINAATDAGADIVRVSVPD
EASSKALKQIVPEVSVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVKEVIKAARDHNCSIRIGVNAGSLEKHL
LDKYGEPCPDAMVESGLDHIKILQDNDFHEFKISCKASDVFMAAAAYQQLADATDAPIHLGITEAGGLVSGTIKSAIGLG
NLLWMGIGDTIRVSLSADPVEEVKVGYEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEKRLEHIRTPMSLSIIGC
VVNGPGEALMTDVGFTGGGAGSGMVYLAGSQSHKLNNDQMIEHIVEQVEKKAAELEAAAAHADAAE

Sequences:

>Translated_386_residues
MTGTVIKVPSMSLNHVRPWRNIYRRASRQIMVGNVPVGGDAPITVQTMTNTLTTDVKATIDQINAATDAGADIVRVSVPD
EASSKALKQIVPEVSVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVKEVIKAARDHNCSIRIGVNAGSLEKHL
LDKYGEPCPDAMVESGLDHIKILQDNDFHEFKISCKASDVFMAAAAYQQLADATDAPIHLGITEAGGLVSGTIKSAIGLG
NLLWMGIGDTIRVSLSADPVEEVKVGYEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEKRLEHIRTPMSLSIIGC
VVNGPGEALMTDVGFTGGGAGSGMVYLAGSQSHKLNNDQMIEHIVEQVEKKAAELEAAAAHADAAE
>Mature_385_residues
TGTVIKVPSMSLNHVRPWRNIYRRASRQIMVGNVPVGGDAPITVQTMTNTLTTDVKATIDQINAATDAGADIVRVSVPDE
ASSKALKQIVPEVSVPIVADIHFHYKRGIEAAEAGAACLRINPGNIGDEKRVKEVIKAARDHNCSIRIGVNAGSLEKHLL
DKYGEPCPDAMVESGLDHIKILQDNDFHEFKISCKASDVFMAAAAYQQLADATDAPIHLGITEAGGLVSGTIKSAIGLGN
LLWMGIGDTIRVSLSADPVEEVKVGYEILKSLGLRHRGVNIISCPSCARQGFDVIKTVETLEKRLEHIRTPMSLSIIGCV
VNGPGEALMTDVGFTGGGAGSGMVYLAGSQSHKLNNDQMIEHIVEQVEKKAAELEAAAAHADAAE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=357, Percent_Identity=58.8235294117647, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 41096; Mature: 40964

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTVIKVPSMSLNHVRPWRNIYRRASRQIMVGNVPVGGDAPITVQTMTNTLTTDVKATI
CCCCEEECCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHH
DQINAATDAGADIVRVSVPDEASSKALKQIVPEVSVPIVADIHFHYKRGIEAAEAGAACL
HHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHCCCEEE
RINPGNIGDEKRVKEVIKAARDHNCSIRIGVNAGSLEKHLLDKYGEPCPDAMVESGLDHI
EECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCEE
KILQDNDFHEFKISCKASDVFMAAAAYQQLADATDAPIHLGITEAGGLVSGTIKSAIGLG
EEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
NLLWMGIGDTIRVSLSADPVEEVKVGYEILKSLGLRHRGVNIISCPSCARQGFDVIKTVE
CEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHH
TLEKRLEHIRTPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGSQSHKLNNDQM
HHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHH
IEHIVEQVEKKAAELEAAAAHADAAE
HHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TGTVIKVPSMSLNHVRPWRNIYRRASRQIMVGNVPVGGDAPITVQTMTNTLTTDVKATI
CCCEEECCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHH
DQINAATDAGADIVRVSVPDEASSKALKQIVPEVSVPIVADIHFHYKRGIEAAEAGAACL
HHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCHHHCCCEEE
RINPGNIGDEKRVKEVIKAARDHNCSIRIGVNAGSLEKHLLDKYGEPCPDAMVESGLDHI
EECCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCCCEE
KILQDNDFHEFKISCKASDVFMAAAAYQQLADATDAPIHLGITEAGGLVSGTIKSAIGLG
EEEECCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
NLLWMGIGDTIRVSLSADPVEEVKVGYEILKSLGLRHRGVNIISCPSCARQGFDVIKTVE
CEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHCHHHHHHHH
TLEKRLEHIRTPMSLSIIGCVVNGPGEALMTDVGFTGGGAGSGMVYLAGSQSHKLNNDQM
HHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHH
IEHIVEQVEKKAAELEAAAAHADAAE
HHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA