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The map label for this gene is dus [H]

Identifier: 110679975

GI number: 110679975

Start: 2638687

End: 2639670

Strand: Reverse

Name: dus [H]

Synonym: RD1_2765

Alternate gene names: 110679975

Gene position: 2639670-2638687 (Counterclockwise)

Preceding gene: 110679980

Following gene: 110679974

Centisome position: 63.87

GC content: 63.01

Gene sequence:

>984_bases
ATGAATTCCGCCGCTTTCAACATAGATCTGAGGCCTCCGGTGCTTTTGGCGCCCATGGCCGGAATCACGGATCGGCCGTT
CCGCGATCTTGTGGCGTCGTTCGGGGCGGGTCTTGTCGTCTCTGAGATGGTAGCGAGCGCGGACATCCTGAATGCGCGCC
CGGGCACCCAGGAAAAGGCGGAGCTTGGTTTATCCGCAGAGGGCACGGCGGTGCAACTGGCAGGGCGCGAACCGGGCTTG
ATGGCCGAAGCTGCGCGCATGGTCGAAGGGCAGGGCGCGCGCATCATTGACATTAACATGGGGTGTCCGGCCAAGAAGGT
CACGCAGGGCTACTCCGGATCCGCCCTGATGCGCACGCCGGATCACGCCATGACGCTGGTCGAGGCGGTGGTGAAGGCCG
TCAACATCCCCGTAACCCTCAAAACACGGCTGGGGTGGGATGATGCGATGCTCAATGCGCCCCAGATCGCGCGCCGTGCC
GAAGAGGCCGGCATCCGGATGCTGACGATTCATGGTCGCACCCGGTGCCAGTTCTACAAAGGTCATGCGGATTGGGCCGC
CATCGCGGGGGTAAAATCCGTGGTCTCGATCCCGGTGATCGCAAATGGTGATATTCTGTGCAGCGCGACCGCCATGGAAG
CGTTGCGTCTGTCCGGAGCGGATGGTGTCATGATCGGGCGTGGCGCACGTGGCAAACCCTGGCTGCTGGCACAGATCGCG
CATGATGTGTACGGCACGCCAGCGCCAGTCGTACCCATGGGCGGGGATTTTGCGGATATGGTGCGGTCGCATTATGCGGC
CATGATCCGGTTTTACGGCCATGATCTGGGCGTCAGGGTCGCGCGCAAACATCTGGGATGGTATATGGACACCTGTTCCG
CGCCCAAGGACATCCGCAAGGCTGTGCTGACCGCGCAAGATGCGGTGACGGTGGACGGGCTGCTGGCGCAAGCCTGTGCC
TGGCCGGATCAGGTCGCCGCATGA

Upstream 100 bases:

>100_bases
TTTAGGCAAACAGGTAAACCGCGCGTCTTTGAGGGCGTTGGATTATTGAGAGAAAAACCCGTAACTGTTAATAAACGGGC
ATTGCTCAAGGATTAAGCAG

Downstream 100 bases:

>100_bases
TGCAGGACAATTCCCTATGGGCAAGCCTGCCGGTGCCGACGCTGATCGTCGGGCCACAAAACAGCATCGTCGATGTGAAT
TCCGCCGCCGAAGGGTTTCT

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: Nitrogen regulation protein nifR3 [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNSAAFNIDLRPPVLLAPMAGITDRPFRDLVASFGAGLVVSEMVASADILNARPGTQEKAELGLSAEGTAVQLAGREPGL
MAEAARMVEGQGARIIDINMGCPAKKVTQGYSGSALMRTPDHAMTLVEAVVKAVNIPVTLKTRLGWDDAMLNAPQIARRA
EEAGIRMLTIHGRTRCQFYKGHADWAAIAGVKSVVSIPVIANGDILCSATAMEALRLSGADGVMIGRGARGKPWLLAQIA
HDVYGTPAPVVPMGGDFADMVRSHYAAMIRFYGHDLGVRVARKHLGWYMDTCSAPKDIRKAVLTAQDAVTVDGLLAQACA
WPDQVAA

Sequences:

>Translated_327_residues
MNSAAFNIDLRPPVLLAPMAGITDRPFRDLVASFGAGLVVSEMVASADILNARPGTQEKAELGLSAEGTAVQLAGREPGL
MAEAARMVEGQGARIIDINMGCPAKKVTQGYSGSALMRTPDHAMTLVEAVVKAVNIPVTLKTRLGWDDAMLNAPQIARRA
EEAGIRMLTIHGRTRCQFYKGHADWAAIAGVKSVVSIPVIANGDILCSATAMEALRLSGADGVMIGRGARGKPWLLAQIA
HDVYGTPAPVVPMGGDFADMVRSHYAAMIRFYGHDLGVRVARKHLGWYMDTCSAPKDIRKAVLTAQDAVTVDGLLAQACA
WPDQVAA
>Mature_327_residues
MNSAAFNIDLRPPVLLAPMAGITDRPFRDLVASFGAGLVVSEMVASADILNARPGTQEKAELGLSAEGTAVQLAGREPGL
MAEAARMVEGQGARIIDINMGCPAKKVTQGYSGSALMRTPDHAMTLVEAVVKAVNIPVTLKTRLGWDDAMLNAPQIARRA
EEAGIRMLTIHGRTRCQFYKGHADWAAIAGVKSVVSIPVIANGDILCSATAMEALRLSGADGVMIGRGARGKPWLLAQIA
HDVYGTPAPVVPMGGDFADMVRSHYAAMIRFYGHDLGVRVARKHLGWYMDTCSAPKDIRKAVLTAQDAVTVDGLLAQACA
WPDQVAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=282, Percent_Identity=28.3687943262411, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI239788462, Length=289, Percent_Identity=28.3737024221453, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=32.0175438596491, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI40807366, Length=275, Percent_Identity=27.2727272727273, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=28.2786885245902, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789660, Length=304, Percent_Identity=44.4078947368421, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI1788462, Length=249, Percent_Identity=36.144578313253, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693211, Length=252, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=231, Percent_Identity=30.7359307359307, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=251, Percent_Identity=28.6852589641434, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=284, Percent_Identity=28.5211267605634, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=28.3261802575107, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=28.448275862069, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=313, Percent_Identity=24.9201277955272, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585320, Length=283, Percent_Identity=31.095406360424, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24580595, Length=279, Percent_Identity=27.5985663082437, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920448, Length=279, Percent_Identity=27.5985663082437, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19921524, Length=292, Percent_Identity=28.4246575342466, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45549423, Length=249, Percent_Identity=28.9156626506024, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34625; Mature: 34625

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAAFNIDLRPPVLLAPMAGITDRPFRDLVASFGAGLVVSEMVASADILNARPGTQEKA
CCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
ELGLSAEGTAVQLAGREPGLMAEAARMVEGQGARIIDINMGCPAKKVTQGYSGSALMRTP
HCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCEEEECC
DHAMTLVEAVVKAVNIPVTLKTRLGWDDAMLNAPQIARRAEEAGIRMLTIHGRTRCQFYK
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHHHHHHCCEEEEEEECCCEEEEEE
GHADWAAIAGVKSVVSIPVIANGDILCSATAMEALRLSGADGVMIGRGARGKPWLLAQIA
CCCCHHHHHHHHHHHEECEEECCCEEEEHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHH
HDVYGTPAPVVPMGGDFADMVRSHYAAMIRFYGHDLGVRVARKHLGWYMDTCSAPKDIRK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AVLTAQDAVTVDGLLAQACAWPDQVAA
HHHHHHHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MNSAAFNIDLRPPVLLAPMAGITDRPFRDLVASFGAGLVVSEMVASADILNARPGTQEKA
CCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHH
ELGLSAEGTAVQLAGREPGLMAEAARMVEGQGARIIDINMGCPAKKVTQGYSGSALMRTP
HCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCCCCEEEECC
DHAMTLVEAVVKAVNIPVTLKTRLGWDDAMLNAPQIARRAEEAGIRMLTIHGRTRCQFYK
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCCHHHHHHHHHCCEEEEEEECCCEEEEEE
GHADWAAIAGVKSVVSIPVIANGDILCSATAMEALRLSGADGVMIGRGARGKPWLLAQIA
CCCCHHHHHHHHHHHEECEEECCCEEEEHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHH
HDVYGTPAPVVPMGGDFADMVRSHYAAMIRFYGHDLGVRVARKHLGWYMDTCSAPKDIRK
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AVLTAQDAVTVDGLLAQACAWPDQVAA
HHHHHHHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8355615 [H]