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The map label for this gene is nifS [H]

Identifier: 110679917

GI number: 110679917

Start: 2570044

End: 2571087

Strand: Reverse

Name: nifS [H]

Synonym: RD1_2698

Alternate gene names: 110679917

Gene position: 2571087-2570044 (Counterclockwise)

Preceding gene: 110679918

Following gene: 110679916

Centisome position: 62.21

GC content: 61.69

Gene sequence:

>1044_bases
GTGAGACGAATTTACCTCGATCACAATGCGACTGCACCGCTGCGCCCGGAGGCGCGCGCGGCGATGCTGGAGGTGATGGA
TCTGCCGGGCAATCCTTCCTCTGTGCACGCAGAAGGGCGCGCGGCCAAGGGTATGATGGAACGCGCACGCCGCGACATTG
CCGAGGCATTGGGGGCGCAGGATGCGGAGATCGTTTTCACGTCCGGCGCCACCGAAGCCGCAGCGCTCGCCTGCGCCGGG
CGCAATCTGCGGGCCAGTGATATCGAACATGACGCGGTGCGTGCATGGTGCCCACCGCTCCTCGATACCGACGCGCAGGG
CGGTGTTACGGTCGATGATCCCGCACAATCGACCCTGCAACGGGCCAATTCGGAAACCGGTATCCTGCAGGATTTGCCAC
AGGACCTCGCCGTCAGCGACATGACACAGGCTTTTGGAAAGATACCGCTCGATTTCACCGCGAGCGGTGCGCGCATGGCG
CTGATCTCGGCCCATAAGCTGGGTGGTCCCAAGGGGATCGGTGCGCTGATCCTGCGGCGCGGGCAGGACATACCGGTGCA
GATCAAAGGCGGCGGGCAGGAGATGGGCCGCCGCTCAGGCACGGAAAACCTGATTGGAATCGCGGGTTTCGCCGCTGCTG
CGCAGGCAGCGCGGCGTGATCTGGACGCCGGACGCTGGGAAGAAATCAAAGAACTTAGAAAGATTCTAGAAAACACCCTT
GCAGCCGAGACAAAGACAACTATTTTTATCGGGAAAGAGGCAAATCGTTTGCCGAACACGTTATGTTTTGCCACGCCGGG
CTGGAAAGGCGAGGCCCAGGTGATCCAGATGGATCTTGCCGGGTTCGCCGTTAGCGCAGGGTCTGCCTGCTCCAGCGGCA
AGGTCAAGGCGAGCAGCGTGTTACGGGCGATGGGGCTGGATGAAGCGACCGCAAGCTGCGCCATTCGCGTGTCCTTGGGT
CTGGAGACGACGAAAACCGAGGTCCTGCAGTTTGCACAGGCCTGGCTTGAAAAAGAAAAGAAATTTCGCGCCCGCGCGGC
GTGA

Upstream 100 bases:

>100_bases
TGAACTCGCACCCTGTCCTGCGGTGCCCAACCTGTTCGACATCGTGGATGAGCCGACAGAACCCGCCTAAAAGCGCGTCC
ACCTTTATGAAAGCCGAGAT

Downstream 100 bases:

>100_bases
GCACTGGAGGATACGATGACAGCATTAGACCAGCCGATCACCGAGGATATTCAGGTCAAAGAAGGCGTTGACCAGGACAC
AGTTGACGCCGTGCGCGAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRRIYLDHNATAPLRPEARAAMLEVMDLPGNPSSVHAEGRAAKGMMERARRDIAEALGAQDAEIVFTSGATEAAALACAG
RNLRASDIEHDAVRAWCPPLLDTDAQGGVTVDDPAQSTLQRANSETGILQDLPQDLAVSDMTQAFGKIPLDFTASGARMA
LISAHKLGGPKGIGALILRRGQDIPVQIKGGGQEMGRRSGTENLIGIAGFAAAAQAARRDLDAGRWEEIKELRKILENTL
AAETKTTIFIGKEANRLPNTLCFATPGWKGEAQVIQMDLAGFAVSAGSACSSGKVKASSVLRAMGLDEATASCAIRVSLG
LETTKTEVLQFAQAWLEKEKKFRARAA

Sequences:

>Translated_347_residues
MRRIYLDHNATAPLRPEARAAMLEVMDLPGNPSSVHAEGRAAKGMMERARRDIAEALGAQDAEIVFTSGATEAAALACAG
RNLRASDIEHDAVRAWCPPLLDTDAQGGVTVDDPAQSTLQRANSETGILQDLPQDLAVSDMTQAFGKIPLDFTASGARMA
LISAHKLGGPKGIGALILRRGQDIPVQIKGGGQEMGRRSGTENLIGIAGFAAAAQAARRDLDAGRWEEIKELRKILENTL
AAETKTTIFIGKEANRLPNTLCFATPGWKGEAQVIQMDLAGFAVSAGSACSSGKVKASSVLRAMGLDEATASCAIRVSLG
LETTKTEVLQFAQAWLEKEKKFRARAA
>Mature_347_residues
MRRIYLDHNATAPLRPEARAAMLEVMDLPGNPSSVHAEGRAAKGMMERARRDIAEALGAQDAEIVFTSGATEAAALACAG
RNLRASDIEHDAVRAWCPPLLDTDAQGGVTVDDPAQSTLQRANSETGILQDLPQDLAVSDMTQAFGKIPLDFTASGARMA
LISAHKLGGPKGIGALILRRGQDIPVQIKGGGQEMGRRSGTENLIGIAGFAAAAQAARRDLDAGRWEEIKELRKILENTL
AAETKTTIFIGKEANRLPNTLCFATPGWKGEAQVIQMDLAGFAVSAGSACSSGKVKASSVLRAMGLDEATASCAIRVSLG
LETTKTEVLQFAQAWLEKEKKFRARAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=31.4285714285714, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI156713448, Length=401, Percent_Identity=31.1720698254364, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI48994898, Length=366, Percent_Identity=33.6065573770492, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25143064, Length=381, Percent_Identity=32.8083989501312, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=28.6604361370716, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=31.6753926701571, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=32.4675324675325, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 36760; Mature: 36760

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIYLDHNATAPLRPEARAAMLEVMDLPGNPSSVHAEGRAAKGMMERARRDIAEALGAQ
CCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DAEIVFTSGATEAAALACAGRNLRASDIEHDAVRAWCPPLLDTDAQGGVTVDDPAQSTLQ
CCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCHHHHHHH
RANSETGILQDLPQDLAVSDMTQAFGKIPLDFTASGARMALISAHKLGGPKGIGALILRR
HCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHC
GQDIPVQIKGGGQEMGRRSGTENLIGIAGFAAAAQAARRDLDAGRWEEIKELRKILENTL
CCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAETKTTIFIGKEANRLPNTLCFATPGWKGEAQVIQMDLAGFAVSAGSACSSGKVKASSV
HCCCCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHEEECCCCCCCCCCHHHHH
LRAMGLDEATASCAIRVSLGLETTKTEVLQFAQAWLEKEKKFRARAA
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRIYLDHNATAPLRPEARAAMLEVMDLPGNPSSVHAEGRAAKGMMERARRDIAEALGAQ
CCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
DAEIVFTSGATEAAALACAGRNLRASDIEHDAVRAWCPPLLDTDAQGGVTVDDPAQSTLQ
CCEEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCHHHHHHH
RANSETGILQDLPQDLAVSDMTQAFGKIPLDFTASGARMALISAHKLGGPKGIGALILRR
HCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCCCCCHHHHHHHC
GQDIPVQIKGGGQEMGRRSGTENLIGIAGFAAAAQAARRDLDAGRWEEIKELRKILENTL
CCCCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAETKTTIFIGKEANRLPNTLCFATPGWKGEAQVIQMDLAGFAVSAGSACSSGKVKASSV
HCCCCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEHHHHEEECCCCCCCCCCHHHHH
LRAMGLDEATASCAIRVSLGLETTKTEVLQFAQAWLEKEKKFRARAA
HHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]