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The map label for this gene is mdeA [H]

Identifier: 110679867

GI number: 110679867

Start: 2519824

End: 2521011

Strand: Direct

Name: mdeA [H]

Synonym: RD1_2640

Alternate gene names: 110679867

Gene position: 2519824-2521011 (Clockwise)

Preceding gene: 110679866

Following gene: 110679868

Centisome position: 60.97

GC content: 62.21

Gene sequence:

>1188_bases
ATGAAAAACGAAAAAGCGTCGGTGATACGGCCTGATGCCCTCCCGCATTCCGCCTCAAGACCTGTGGTCACACCGCTGAG
CCCGTCGGTTGTTTACGCCTCGGACACACCGGACATGCTGGACCAGCAGTATGAAGGCGGGTTGCAGGGCTATACCTATT
CGCGCGAAGGGCACCCGAATGCGGATGTGGTGGCCAAACGGCTCGACCGAATGGAAAATGCGCCCTTTCCGGGGGTGATG
ACCGGGTCCGGCATGGGGGCTGTGTCCGCGGTTTTGCTGGGGCTGACACGCTCCGGGGACCATGTGATCGGCGGCAATCA
GCTATATGGTCGCTCGCTGCGCATGACGAGCGAAGACCTGCCCCGGCTTGGCATTGCCACGACTTTGGTGGACGCGGGCG
ACATCGACGCTGTGCGCGCAGCCTTGCGTCCCACGACCCGGATGATCCTCGTCGAAGTTGTTTCAAACCCGACACTGCGG
CTTGCGGATATCGCGGGTCTTGTTGATCTGGCGCGTGAATCTGGTGTGCTGCTGGTGGTGGACAACACATTTACCACTCC
GATGGCCTTCAAGGCGCTGGATGCGGGTGCGGATGTGGTGATCCATTCGGTGACCAAGCTGCTGGCAGGTCATTCCGATG
TGATGCTGGGCTATGTTGCGGCGCGGGACAAAGCGTTGAACGACCGCTTGCGCGTCTTTGCCGTGACCATGGGGCTCTCG
GCCAGTCCGTTTGACTGCTGGCTGGCGGAGCGCGGTTTGCTAACTCTTGAGTTGCGGTTCGAGCGGGCGCAACGCACGGC
GGCTCTTCTGGCCGATCATCTTGCCGGTCTTCCGGGTGTCAAACGGGTGCTTTATCCCGGGCGCGCCGACCACCCGGACA
CCCTGCGGGCAAAGGCGCTGCTGAAGGGCAGGAACTGCAATATGGTCAGCTTTGAACTGGATGGCGCGCGGCACGCGGCG
AATGCCTTTACCCGTGCGGCAGAGGGGTTGTCTTTTGCGCCAACCCTCGGGGATGTGGGCACGACGCTGTCGCATCCTGC
GTCCTCGTCGCACCGGGCACTGAGCCCCGAGCAACGCGCCGCCCTTGGGATATCGGAAGGGTTTTTTCGTGTCTCTGTCG
GGCTCGAAGACCCCGATAATCTCTGCGCGATCTTTACGGAAGCGGTCAGCGCGGCTGTGGCCCCCTAG

Upstream 100 bases:

>100_bases
CTGGATGCGGCTGCGATTGAGGGGCTGAGGAACTCGGCGCTGCACTACCAGAAAAACATGCGTCGCTTCAAACATGAGAT
TCGCGCTATAGAGATTTGAT

Downstream 100 bases:

>100_bases
CAAGGATTAAAAACACCCATGGCAGAGGCAATTCACGAGGTGCTCGCGGCCCTGCCGATGCCGGTTTTGGCCATAGGTGG
CAGCGAACGCATCGTGTCGG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKNEKASVIRPDALPHSASRPVVTPLSPSVVYASDTPDMLDQQYEGGLQGYTYSREGHPNADVVAKRLDRMENAPFPGVM
TGSGMGAVSAVLLGLTRSGDHVIGGNQLYGRSLRMTSEDLPRLGIATTLVDAGDIDAVRAALRPTTRMILVEVVSNPTLR
LADIAGLVDLARESGVLLVVDNTFTTPMAFKALDAGADVVIHSVTKLLAGHSDVMLGYVAARDKALNDRLRVFAVTMGLS
ASPFDCWLAERGLLTLELRFERAQRTAALLADHLAGLPGVKRVLYPGRADHPDTLRAKALLKGRNCNMVSFELDGARHAA
NAFTRAAEGLSFAPTLGDVGTTLSHPASSSHRALSPEQRAALGISEGFFRVSVGLEDPDNLCAIFTEAVSAAVAP

Sequences:

>Translated_395_residues
MKNEKASVIRPDALPHSASRPVVTPLSPSVVYASDTPDMLDQQYEGGLQGYTYSREGHPNADVVAKRLDRMENAPFPGVM
TGSGMGAVSAVLLGLTRSGDHVIGGNQLYGRSLRMTSEDLPRLGIATTLVDAGDIDAVRAALRPTTRMILVEVVSNPTLR
LADIAGLVDLARESGVLLVVDNTFTTPMAFKALDAGADVVIHSVTKLLAGHSDVMLGYVAARDKALNDRLRVFAVTMGLS
ASPFDCWLAERGLLTLELRFERAQRTAALLADHLAGLPGVKRVLYPGRADHPDTLRAKALLKGRNCNMVSFELDGARHAA
NAFTRAAEGLSFAPTLGDVGTTLSHPASSSHRALSPEQRAALGISEGFFRVSVGLEDPDNLCAIFTEAVSAAVAP
>Mature_395_residues
MKNEKASVIRPDALPHSASRPVVTPLSPSVVYASDTPDMLDQQYEGGLQGYTYSREGHPNADVVAKRLDRMENAPFPGVM
TGSGMGAVSAVLLGLTRSGDHVIGGNQLYGRSLRMTSEDLPRLGIATTLVDAGDIDAVRAALRPTTRMILVEVVSNPTLR
LADIAGLVDLARESGVLLVVDNTFTTPMAFKALDAGADVVIHSVTKLLAGHSDVMLGYVAARDKALNDRLRVFAVTMGLS
ASPFDCWLAERGLLTLELRFERAQRTAALLADHLAGLPGVKRVLYPGRADHPDTLRAKALLKGRNCNMVSFELDGARHAA
NAFTRAAEGLSFAPTLGDVGTTLSHPASSSHRALSPEQRAALGISEGFFRVSVGLEDPDNLCAIFTEAVSAAVAP

Specific function: Catalyzes the transformation of cystathionine into homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=32.5396825396825, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=30.6068601583113, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=28.3819628647215, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1790375, Length=344, Percent_Identity=38.6627906976744, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1789383, Length=310, Percent_Identity=27.741935483871, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17538055, Length=358, Percent_Identity=34.6368715083799, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=33.5195530726257, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71980708, Length=381, Percent_Identity=32.0209973753281, Blast_Score=196, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=377, Percent_Identity=32.0954907161804, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=384, Percent_Identity=27.34375, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=25.5924170616114, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=250, Percent_Identity=31.2, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=322, Percent_Identity=23.9130434782609, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922554, Length=385, Percent_Identity=35.0649350649351, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41886; Mature: 41886

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEKASVIRPDALPHSASRPVVTPLSPSVVYASDTPDMLDQQYEGGLQGYTYSREGHPN
CCCCCCCEECCCCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHCCCCCCEEECCCCCCC
ADVVAKRLDRMENAPFPGVMTGSGMGAVSAVLLGLTRSGDHVIGGNQLYGRSLRMTSEDL
HHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCEEECCCCEECCEEECCHHCC
PRLGIATTLVDAGDIDAVRAALRPTTRMILVEVVSNPTLRLADIAGLVDLARESGVLLVV
CCCCHHHHEECCCCHHHHHHHHCCHHHEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEE
DNTFTTPMAFKALDAGADVVIHSVTKLLAGHSDVMLGYVAARDKALNDRLRVFAVTMGLS
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHCCEEEEEEEECCC
ASPFDCWLAERGLLTLELRFERAQRTAALLADHLAGLPGVKRVLYPGRADHPDTLRAKAL
CCCHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHH
LKGRNCNMVSFELDGARHAANAFTRAAEGLSFAPTLGDVGTTLSHPASSSHRALSPEQRA
HCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALGISEGFFRVSVGLEDPDNLCAIFTEAVSAAVAP
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNEKASVIRPDALPHSASRPVVTPLSPSVVYASDTPDMLDQQYEGGLQGYTYSREGHPN
CCCCCCCEECCCCCCCCCCCCEECCCCCCEEEECCCHHHHHHHHCCCCCCEEECCCCCCC
ADVVAKRLDRMENAPFPGVMTGSGMGAVSAVLLGLTRSGDHVIGGNQLYGRSLRMTSEDL
HHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHCCCCCEEECCCCEECCEEECCHHCC
PRLGIATTLVDAGDIDAVRAALRPTTRMILVEVVSNPTLRLADIAGLVDLARESGVLLVV
CCCCHHHHEECCCCHHHHHHHHCCHHHEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEE
DNTFTTPMAFKALDAGADVVIHSVTKLLAGHSDVMLGYVAARDKALNDRLRVFAVTMGLS
CCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCHHEEEHHHHHHHHHHCCEEEEEEEECCC
ASPFDCWLAERGLLTLELRFERAQRTAALLADHLAGLPGVKRVLYPGRADHPDTLRAKAL
CCCHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHH
LKGRNCNMVSFELDGARHAANAFTRAAEGLSFAPTLGDVGTTLSHPASSSHRALSPEQRA
HCCCCCCEEEEEECCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALGISEGFFRVSVGLEDPDNLCAIFTEAVSAAVAP
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]