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The map label for this gene is potB [H]

Identifier: 110679675

GI number: 110679675

Start: 2315969

End: 2316820

Strand: Direct

Name: potB [H]

Synonym: RD1_2429

Alternate gene names: 110679675

Gene position: 2315969-2316820 (Clockwise)

Preceding gene: 110679674

Following gene: 110679676

Centisome position: 56.03

GC content: 59.74

Gene sequence:

>852_bases
ATGGCTGGTGCCGCAACATATGAACCCGGACGTGTGGACTGGCGGCTCGCAGCGCCGCTCGGTCTGACGTATCTGGCGTT
TTTTGCGGCACCGCTCCTTATCCTGCTGGCGATGTCGTTTGCGGCGGATGATCGTTTTGCGTCCTTCTCTGCGGACAGTT
GGGTCAAGTTCTGGGGCGACCCGTTCTATCGCGGCGTTGTCTATGACACGGTGTGGCTTGGTCTGATCGCCGTGGTGGCG
ACGTCGATCCTGTCCTATCCGATTGCGCTTCTGTTCATTTCGTGCGGGCCGCGCCTGCGGAAACTTCTGATCTTCATCAT
CCTGCTTCCGCTGCTGACATCGGTGGTGATCCGCACGTTCGCTTGGATCGTGATCCTCGCGCGCGAGGGCGTGGTCAATC
AGACCCTGATCAGCCTCGGGCTGACTTCTGCGCCGCTGAACCTTTTGCAAACAGAACTGGGCCTCGTGATTGCGCTGACG
CAGATCGAGATGCCCCTGATGCTGTTACCGCTCGTCACGGTCATGCAGCAAATCAACCCCAGCCTGTCGGATGCGTCCCG
CGCGCTTGGCGGTTCCAAATGGCGCACATTCTTCCGCATCATCCTGCCGCTTTCGCTGCCCGGATGGATTGCGGGCGCAA
CCCTTGTGTTCGCCTCGGCGACAACCGCGTTCATCTCGCAGACGGTGATTGGTGGCGCGCGTCTTGTCTATCTGCCGTCG
CTGATCTGGCAGCAATCGATGGTCGTTTTCGACTGGCCCTTTGCCGCCGTGGCATCGGTTACACTGCTGGTTGCTGTGCT
CAGCGGCGTGATGCTGTTTTCCCTGCTTGGTCGTTTTGCGAGGATAGACTGA

Upstream 100 bases:

>100_bases
TGGATGAGTTGTCGGATATGGTTGTGCACGATTGGAGCGTGATCAATCCGCAGCGTGCGGGGTGGATTGAGCGGTTCAAC
AAAGAGATCACCAAGTAATC

Downstream 100 bases:

>100_bases
TGGCGCATCGCAGCACCGTTTCGGACCGGTCCTATTCCATTGTCATGGGCGGCCTTGCAGCACTTGGCATCCTGATCCTT
GTGGGTCCCGTGATCATCGT

Product: ABC transporter protein, permease component, putative

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAGAATYEPGRVDWRLAAPLGLTYLAFFAAPLLILLAMSFAADDRFASFSADSWVKFWGDPFYRGVVYDTVWLGLIAVVA
TSILSYPIALLFISCGPRLRKLLIFIILLPLLTSVVIRTFAWIVILAREGVVNQTLISLGLTSAPLNLLQTELGLVIALT
QIEMPLMLLPLVTVMQQINPSLSDASRALGGSKWRTFFRIILPLSLPGWIAGATLVFASATTAFISQTVIGGARLVYLPS
LIWQQSMVVFDWPFAAVASVTLLVAVLSGVMLFSLLGRFARID

Sequences:

>Translated_283_residues
MAGAATYEPGRVDWRLAAPLGLTYLAFFAAPLLILLAMSFAADDRFASFSADSWVKFWGDPFYRGVVYDTVWLGLIAVVA
TSILSYPIALLFISCGPRLRKLLIFIILLPLLTSVVIRTFAWIVILAREGVVNQTLISLGLTSAPLNLLQTELGLVIALT
QIEMPLMLLPLVTVMQQINPSLSDASRALGGSKWRTFFRIILPLSLPGWIAGATLVFASATTAFISQTVIGGARLVYLPS
LIWQQSMVVFDWPFAAVASVTLLVAVLSGVMLFSLLGRFARID
>Mature_282_residues
AGAATYEPGRVDWRLAAPLGLTYLAFFAAPLLILLAMSFAADDRFASFSADSWVKFWGDPFYRGVVYDTVWLGLIAVVAT
SILSYPIALLFISCGPRLRKLLIFIILLPLLTSVVIRTFAWIVILAREGVVNQTLISLGLTSAPLNLLQTELGLVIALTQ
IEMPLMLLPLVTVMQQINPSLSDASRALGGSKWRTFFRIILPLSLPGWIAGATLVFASATTAFISQTVIGGARLVYLPSL
IWQQSMVVFDWPFAAVASVTLLVAVLSGVMLFSLLGRFARID

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=250, Percent_Identity=32.8, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787080, Length=237, Percent_Identity=29.957805907173, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787713, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30811; Mature: 30680

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAATYEPGRVDWRLAAPLGLTYLAFFAAPLLILLAMSFAADDRFASFSADSWVKFWGD
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHCC
PFYRGVVYDTVWLGLIAVVATSILSYPIALLFISCGPRLRKLLIFIILLPLLTSVVIRTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AWIVILAREGVVNQTLISLGLTSAPLNLLQTELGLVIALTQIEMPLMLLPLVTVMQQINP
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLSDASRALGGSKWRTFFRIILPLSLPGWIAGATLVFASATTAFISQTVIGGARLVYLPS
CHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIWQQSMVVFDWPFAAVASVTLLVAVLSGVMLFSLLGRFARID
HHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGAATYEPGRVDWRLAAPLGLTYLAFFAAPLLILLAMSFAADDRFASFSADSWVKFWGD
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHCC
PFYRGVVYDTVWLGLIAVVATSILSYPIALLFISCGPRLRKLLIFIILLPLLTSVVIRTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AWIVILAREGVVNQTLISLGLTSAPLNLLQTELGLVIALTQIEMPLMLLPLVTVMQQINP
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLSDASRALGGSKWRTFFRIILPLSLPGWIAGATLVFASATTAFISQTVIGGARLVYLPS
CHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LIWQQSMVVFDWPFAAVASVTLLVAVLSGVMLFSLLGRFARID
HHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]