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The map label for this gene is ntaB [H]

Identifier: 110679586

GI number: 110679586

Start: 2228403

End: 2228924

Strand: Reverse

Name: ntaB [H]

Synonym: RD1_2331

Alternate gene names: 110679586

Gene position: 2228924-2228403 (Counterclockwise)

Preceding gene: 110679587

Following gene: 110679577

Centisome position: 53.93

GC content: 60.15

Gene sequence:

>522_bases
GTGAGCGCGGCCCATGCTTTCGAGCCGAGCCCGGATCATAGCCGTGACCTGCGCACCGCATTCGGGTGTTTTGGAACCGG
GGTGACCGTGATCTGCGCCCAGACGCCCGATGGACCGGTGGGGATGACGGCCAACTCGTTTTCCGCCATATCGCTTGACC
CGCCGCTTGTGCTTTGGGCACCGTCCAGCAGTTCGAAACGGCATGCGGCCTTTGTGAGCGCTGAACACTTCTGCGTTCAC
ATCCTCGCAGCGGATCAACTCAGCATGGCGCGCCATTTCGCCAGTAACGGCACGGATTTTGACGCCTTTGATTGGTATAA
GGGGCCTTTAGGCGCCCCTACGCTGCGCGGATGCATTGCGGAGTTTCATTGCGACACGGATGCGATCCACCCGGCCGGCG
ATCACTCCCTGATCCTTGGGCTTGTGCGCCGCGTTGTACACCGCGCCAATGGCAAGAACGGTCTGTTGTTTGATCAGAGC
CGTTTTGGACAGTTTTCGCCCACCGGACCTGCCGATCAATAA

Upstream 100 bases:

>100_bases
TGACAGGTTTCGAATTTATATCGTCACACTCCGAGCGTCATTGGGAATGCGGTATCAGAATGTGCGAACTCAAATCTCTG
CGTCAACAGCGGTTTCAAAC

Downstream 100 bases:

>100_bases
TCCCGTTCGAAAAAGAAGCCCACACTGCTGTCGCCATCCTCAGCCACGGTCGCCTTGCCTTTGAGGTCGCTGGTCAGATC
GATGTTCAGCGAGATATCCG

Product: nitrilotriacetate monooxygenase component B

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSAAHAFEPSPDHSRDLRTAFGCFGTGVTVICAQTPDGPVGMTANSFSAISLDPPLVLWAPSSSSKRHAAFVSAEHFCVH
ILAADQLSMARHFASNGTDFDAFDWYKGPLGAPTLRGCIAEFHCDTDAIHPAGDHSLILGLVRRVVHRANGKNGLLFDQS
RFGQFSPTGPADQ

Sequences:

>Translated_173_residues
MSAAHAFEPSPDHSRDLRTAFGCFGTGVTVICAQTPDGPVGMTANSFSAISLDPPLVLWAPSSSSKRHAAFVSAEHFCVH
ILAADQLSMARHFASNGTDFDAFDWYKGPLGAPTLRGCIAEFHCDTDAIHPAGDHSLILGLVRRVVHRANGKNGLLFDQS
RFGQFSPTGPADQ
>Mature_172_residues
SAAHAFEPSPDHSRDLRTAFGCFGTGVTVICAQTPDGPVGMTANSFSAISLDPPLVLWAPSSSSKRHAAFVSAEHFCVHI
LAADQLSMARHFASNGTDFDAFDWYKGPLGAPTLRGCIAEFHCDTDAIHPAGDHSLILGLVRRVVHRANGKNGLLFDQSR
FGQFSPTGPADQ

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=26.1146496815287, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 18449; Mature: 18318

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAHAFEPSPDHSRDLRTAFGCFGTGVTVICAQTPDGPVGMTANSFSAISLDPPLVLWA
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEC
PSSSSKRHAAFVSAEHFCVHILAADQLSMARHFASNGTDFDAFDWYKGPLGAPTLRGCIA
CCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
EFHCDTDAIHPAGDHSLILGLVRRVVHRANGKNGLLFDQSRFGQFSPTGPADQ
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAAHAFEPSPDHSRDLRTAFGCFGTGVTVICAQTPDGPVGMTANSFSAISLDPPLVLWA
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEC
PSSSSKRHAAFVSAEHFCVHILAADQLSMARHFASNGTDFDAFDWYKGPLGAPTLRGCIA
CCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
EFHCDTDAIHPAGDHSLILGLVRRVVHRANGKNGLLFDQSRFGQFSPTGPADQ
HHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]