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The map label for this gene is phrB [H]

Identifier: 110679555

GI number: 110679555

Start: 2189803

End: 2191221

Strand: Direct

Name: phrB [H]

Synonym: RD1_2295

Alternate gene names: 110679555

Gene position: 2189803-2191221 (Clockwise)

Preceding gene: 110679554

Following gene: 110679556

Centisome position: 52.98

GC content: 61.73

Gene sequence:

>1419_bases
TTGAGCACATCTCCCGTTTTGGTCTGGTTCCGGCGTGATCTGCGCCTGAGCGATCACCCCGCATTGGACGCTGCCGTCAA
ATCCGGCAATCCCGTCATTCCCGTATTCATCCACGATGATCAGGTTGAGACGCTGGGCGCGGCGCCCAGATGGCGTCTGG
GTCTGGGGATCGGACATCTGTCAGAAACGCTGGAGGGGCTCAACAGCCGCTTGATCCTGCGGCGCGGTCCGGCGCTTGAA
ACGCTTCGCACGCTCATCCTTGAAACCGGGGCAGGGGCTGTGTTCTGGTCGCGCCTCTATGATCCGGCGGCGGTCGAACG
CGACACGGAGGTGAAGGCGGCGCTCAAGCAGGACGGCATCGACGCGCGCTCTTTCGGGGGGCATCTGATGTTTGAACCCT
GGACCGTGGAGACCAAGACGGGCGGGTTCTACAAAGTCTACACGCCATTTTGGAACATGGTCAAAAACCGTGATGTTGAC
GCGCCCCTTTCGGCCCCGTCCAGAATTGACGCACCTGCGGCATGGCCGGGTAGCGATACGCTCAGCGATTGGAACATGGG
CGCTGCGATGGATCGTGGCGCTGCGGTTGTTGCACCCTACGTTCGCTTGGGGGAGGCCGCCGCACAGACGCGCCTGAACA
CGTTCATCGACAAAATCGTCGCAGGTTATGACACCACCCGCGATCAGCCCGCAATCGATGGCACATCGGGGCTGTCGGAA
AACCTCGCCCTTGGCGAGATCAGCCCGCATCAATGCTGGTATGCCGGGCTGCGCGCGCGCGAGGAGGAGAAACCGGGGGC
CGAGACGTTCCTCAAGGAACTGGTCTGGCGCGAATTTGCCTATCACCTGATGCACCACACGCCGCGCATCCTGACGGGCA
ACTGGAAAGAAGACTGGGACAGCTTCCCGTGGAACCGGGATGAAACGACGCAGGCCGTCCGGCGCTGGCAACAGGGCCGC
ACCGGTATCCAGTTTGTCGATGCCGCCATGCGCGAGATGTATGTGACGGGGCGGATGCACAACCGGGGCCGCATGATCGT
GGCGAGTTACCTGACCAAACACCTTCTCAGCGATTGGAAGATCGGGTTGAAGTGGTTTGAGGACTGTCTCATCGACTGGG
ACCCGGCCAGCAACGCCATGGGCTGGCAGTGGTCGGCGGGGTCCGGACCCGACGCCACCCCGTATTTCAGAGTGTTCAAC
CCCGTCACCCAGTTGGATAAGTTCGACAAGAACCGCCAGTATGTAAAGCGCTGGGTTGCCGAGGAGCGGCGTGCGCCGAC
GCAAACCGCGCTGTCCTATTTCGACGCGATCCCGCGGGCCTGGGGCCTGAGTGCGGATCAGGATTATCCCGCTCCGGTTG
TGGCGGCGGATGAGGGGCGCAAGCGTGCTTTGGAAGCCTATGAAAATAGAGGGTTTTAA

Upstream 100 bases:

>100_bases
CAGTTGATTGACGCTTTGGACAAGGTATTGTGAGCGGCGCGACTGATCTAACTCTGAGGCCCGCGCGTAAAACCTGCAAT
AAATTCAGAAGGGTTACGGT

Downstream 100 bases:

>100_bases
AACTTTGAAAGGCTTGATCACAGCCCTAACTCTGTCCTAATAAACTGTTAACTAACGGCAAGAAACGAGCATTTCATTTA
TGATCCTGACATCGACAGAC

Product: deoxyribodipyrimidine photolyase, putative

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSTSPVLVWFRRDLRLSDHPALDAAVKSGNPVIPVFIHDDQVETLGAAPRWRLGLGIGHLSETLEGLNSRLILRRGPALE
TLRTLILETGAGAVFWSRLYDPAAVERDTEVKAALKQDGIDARSFGGHLMFEPWTVETKTGGFYKVYTPFWNMVKNRDVD
APLSAPSRIDAPAAWPGSDTLSDWNMGAAMDRGAAVVAPYVRLGEAAAQTRLNTFIDKIVAGYDTTRDQPAIDGTSGLSE
NLALGEISPHQCWYAGLRAREEEKPGAETFLKELVWREFAYHLMHHTPRILTGNWKEDWDSFPWNRDETTQAVRRWQQGR
TGIQFVDAAMREMYVTGRMHNRGRMIVASYLTKHLLSDWKIGLKWFEDCLIDWDPASNAMGWQWSAGSGPDATPYFRVFN
PVTQLDKFDKNRQYVKRWVAEERRAPTQTALSYFDAIPRAWGLSADQDYPAPVVAADEGRKRALEAYENRGF

Sequences:

>Translated_472_residues
MSTSPVLVWFRRDLRLSDHPALDAAVKSGNPVIPVFIHDDQVETLGAAPRWRLGLGIGHLSETLEGLNSRLILRRGPALE
TLRTLILETGAGAVFWSRLYDPAAVERDTEVKAALKQDGIDARSFGGHLMFEPWTVETKTGGFYKVYTPFWNMVKNRDVD
APLSAPSRIDAPAAWPGSDTLSDWNMGAAMDRGAAVVAPYVRLGEAAAQTRLNTFIDKIVAGYDTTRDQPAIDGTSGLSE
NLALGEISPHQCWYAGLRAREEEKPGAETFLKELVWREFAYHLMHHTPRILTGNWKEDWDSFPWNRDETTQAVRRWQQGR
TGIQFVDAAMREMYVTGRMHNRGRMIVASYLTKHLLSDWKIGLKWFEDCLIDWDPASNAMGWQWSAGSGPDATPYFRVFN
PVTQLDKFDKNRQYVKRWVAEERRAPTQTALSYFDAIPRAWGLSADQDYPAPVVAADEGRKRALEAYENRGF
>Mature_471_residues
STSPVLVWFRRDLRLSDHPALDAAVKSGNPVIPVFIHDDQVETLGAAPRWRLGLGIGHLSETLEGLNSRLILRRGPALET
LRTLILETGAGAVFWSRLYDPAAVERDTEVKAALKQDGIDARSFGGHLMFEPWTVETKTGGFYKVYTPFWNMVKNRDVDA
PLSAPSRIDAPAAWPGSDTLSDWNMGAAMDRGAAVVAPYVRLGEAAAQTRLNTFIDKIVAGYDTTRDQPAIDGTSGLSEN
LALGEISPHQCWYAGLRAREEEKPGAETFLKELVWREFAYHLMHHTPRILTGNWKEDWDSFPWNRDETTQAVRRWQQGRT
GIQFVDAAMREMYVTGRMHNRGRMIVASYLTKHLLSDWKIGLKWFEDCLIDWDPASNAMGWQWSAGSGPDATPYFRVFNP
VTQLDKFDKNRQYVKRWVAEERRAPTQTALSYFDAIPRAWGLSADQDYPAPVVAADEGRKRALEAYENRGF

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=479, Percent_Identity=25.2609603340292, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI4758072, Length=484, Percent_Identity=25.8264462809917, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI188536103, Length=434, Percent_Identity=23.5023041474654, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786926, Length=480, Percent_Identity=34.1666666666667, Blast_Score=232, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324962, Length=519, Percent_Identity=26.9749518304432, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137248, Length=502, Percent_Identity=27.8884462151394, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24585455, Length=502, Percent_Identity=27.8884462151394, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24648152, Length=512, Percent_Identity=23.6328125, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53291; Mature: 53159

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPVLVWFRRDLRLSDHPALDAAVKSGNPVIPVFIHDDQVETLGAAPRWRLGLGIGHL
CCCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHCCCCCEEECCCHHHH
SETLEGLNSRLILRRGPALETLRTLILETGAGAVFWSRLYDPAAVERDTEVKAALKQDGI
HHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCHHHHHHHHHCCC
DARSFGGHLMFEPWTVETKTGGFYKVYTPFWNMVKNRDVDAPLSAPSRIDAPAAWPGSDT
CHHHCCCEEEECCEEEEECCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
LSDWNMGAAMDRGAAVVAPYVRLGEAAAQTRLNTFIDKIVAGYDTTRDQPAIDGTSGLSE
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NLALGEISPHQCWYAGLRAREEEKPGAETFLKELVWREFAYHLMHHTPRILTGNWKEDWD
CCEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHC
SFPWNRDETTQAVRRWQQGRTGIQFVDAAMREMYVTGRMHNRGRMIVASYLTKHLLSDWK
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IGLKWFEDCLIDWDPASNAMGWQWSAGSGPDATPYFRVFNPVTQLDKFDKNRQYVKRWVA
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
EERRAPTQTALSYFDAIPRAWGLSADQDYPAPVVAADEGRKRALEAYENRGF
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
STSPVLVWFRRDLRLSDHPALDAAVKSGNPVIPVFIHDDQVETLGAAPRWRLGLGIGHL
CCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEECCCCHHHHCCCCCEEECCCHHHH
SETLEGLNSRLILRRGPALETLRTLILETGAGAVFWSRLYDPAAVERDTEVKAALKQDGI
HHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCHHHHHHHHHCCC
DARSFGGHLMFEPWTVETKTGGFYKVYTPFWNMVKNRDVDAPLSAPSRIDAPAAWPGSDT
CHHHCCCEEEECCEEEEECCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
LSDWNMGAAMDRGAAVVAPYVRLGEAAAQTRLNTFIDKIVAGYDTTRDQPAIDGTSGLSE
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
NLALGEISPHQCWYAGLRAREEEKPGAETFLKELVWREFAYHLMHHTPRILTGNWKEDWD
CCEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHC
SFPWNRDETTQAVRRWQQGRTGIQFVDAAMREMYVTGRMHNRGRMIVASYLTKHLLSDWK
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
IGLKWFEDCLIDWDPASNAMGWQWSAGSGPDATPYFRVFNPVTQLDKFDKNRQYVKRWVA
HHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
EERRAPTQTALSYFDAIPRAWGLSADQDYPAPVVAADEGRKRALEAYENRGF
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2837735; 2110564; 9360600 [H]