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The map label for this gene is opuD [C]

Identifier: 110679552

GI number: 110679552

Start: 2184559

End: 2186397

Strand: Reverse

Name: opuD [C]

Synonym: RD1_2291

Alternate gene names: 110679552

Gene position: 2186397-2184559 (Counterclockwise)

Preceding gene: 110679561

Following gene: 110679546

Centisome position: 52.9

GC content: 58.95

Gene sequence:

>1839_bases
ATGACAGTCAAACCTCCATTTACGGATCTTGAGATCAACACTCAGGACTCCGGCTTTTACGAAGGCTACAGCCTACCCAT
CGCGCTGGTCTCAAAGATCGGCATGGTGCTTCTGGTCCTCTGGGCCCTCGTCTTTCCTGCCGGTGCAGGGGCGATCTTGG
CAGAGGTCAATGGCACCTTGCTGAACTCATTCAATACTTTCTACATCCTCTCGGTGGGTGCCTTTGCCTTTTTCCTCTTT
TTAGTGGCCATCATCCCCGCAACCGGGAAACGTGTTCTCGGCATGTCCGGTCAAAAGCCCGAGTTTTCCAATTTCAGCTG
GTTTTCCATGATGTTCGGCGCAGGTCTCGGCGTGGGGTTGATGACATTTGCCACCGCCGAACCGCTCGGCCTTTGGGGAT
CAAACCCGTCGGTGGTCTCGCGTGAGGTGGCCGGCAACACGCCCGAAGCGCTGCAATCCACGTTCCGCTATACCTTCCTG
CATTACGGGTTTCATGCCTGGGCCATTTATGTGGTGACGGGGCTGAGCCTTGCCTATTACGCCTATACAAGGGGCATGCC
GCTGACGATCCGTTCGGCCCTGACCCCGCTGATCGGGCGCTTTGCGAATGGTGCCTTTGGTCATTTGATCGACGTTCTGG
GTGTTGTTGCGACCATCCTTGGCGTATCGGTCACAATCGGTTTTGGCGTCAGCCAGCTGGTGGATGGTGTGTTTTCCATC
ACCGGTGCCGAATGGATGATGGACACCACTGGCGATACGCCGGTTGCGACCAAGACGGGCCTGATCGCCGCCCTTGTGGT
CATCATGGGCATGTCGATCATCTCGGCTGTGTCGGGTGTGGGGCGCGGTGTGAAATACCTGTCGAACCTCAACCTTGTCC
TGTCGCTGATCCTGTTGATGACATTCGTGATTTTCGGGTCGTTCTTTTTCGCCATGACGACCTATGCGACCGCGCTGATC
GACTATCTGCTGCATTTTGTGAACCTCAGCTTTGAGGCCTACGCACCGCTGGCGCAAGACGCTTTTGCGACCGCAGCGCC
CGCGTCTGTGCAGGCCCTGCCCGCCGAAGAACTCGCCGCCGTTTATGCGGGTGCCACAAACCAGTGGGGCAGCTATGCAG
GCTTTACTGCGGGGCTTGAAGGGGCCGCTGCCGCCCTGCCCGCCGCCGATCTGGACGCCGCCTATGCGGCTGGCGAACAG
GGCCGTCTATTCGGGTGGCAGGCCGGATGGACGACATTCTACTGGGCGTGGTGGATTGCATTCTCGCCCTTTGTCGGATT
GTTCCTTGCCCGTATCTCGCGGGGACGCACGGTGCGTGAATTTGTGCTGGGTGCGGTCTTTGCACCGGCACTGGTGTGTT
TTGCGTGGATGACGATCCTCGGCGGTACGGCCATTGATCTTGAAATCAGCGGTGCTGCTGAGGGCGCAATCATTGCGGCG
TCAAATTCGGCCAAGCTCTTCGTCACGCTGGAGCAGATGCTGTCGGGCGCGGTGCTTCAGGCGCTGATCATCATGTGCGT
TGTGCTGATCATGACCTTTCTCGTGACCTCGGCGGATTCCGGTATTCTGGTCATGAATACGATCATGTCCGGCGGGGCCG
AGGAAACCGGCGTCAAGCACCGGATCATCTGGGGTATCATCCTGACGCTGGTGATCGGCACCTTGCTTCTGGCGGGGGGT
GGCGGGCTGTCCGCCCTGCAAAACGCCATGATCATCGGGGCGCTGCCCTTCACCATGATTATGGTGCTGATGGTCTTGTC
GCTGATCAAGGCGCTGTTCAACGATGGTCGCCGTGAAAGAGCGGGCGTGCGCCCGGCAGCCGCGCAGCCAGCCGAATAG

Upstream 100 bases:

>100_bases
AAAAACACGCATGGCGCGAAAAATGGACTCATTTATGCTAAACTCAGACTGAGATCAAAAAACGACGCGAAACGCGGTTT
TCGAAAACAGGGAAACAAAA

Downstream 100 bases:

>100_bases
GTTCGGCCTGCACACGTAAAAAGCGCGCCACACCGTGACGCGCTTTTTCATCGGATGTGTACGCTTTGCCCGCGCGTAGG
CCTTGTCCTTGGCTACCGGG

Product: glycine betaine transporter OpuD, putative

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MTVKPPFTDLEINTQDSGFYEGYSLPIALVSKIGMVLLVLWALVFPAGAGAILAEVNGTLLNSFNTFYILSVGAFAFFLF
LVAIIPATGKRVLGMSGQKPEFSNFSWFSMMFGAGLGVGLMTFATAEPLGLWGSNPSVVSREVAGNTPEALQSTFRYTFL
HYGFHAWAIYVVTGLSLAYYAYTRGMPLTIRSALTPLIGRFANGAFGHLIDVLGVVATILGVSVTIGFGVSQLVDGVFSI
TGAEWMMDTTGDTPVATKTGLIAALVVIMGMSIISAVSGVGRGVKYLSNLNLVLSLILLMTFVIFGSFFFAMTTYATALI
DYLLHFVNLSFEAYAPLAQDAFATAAPASVQALPAEELAAVYAGATNQWGSYAGFTAGLEGAAAALPAADLDAAYAAGEQ
GRLFGWQAGWTTFYWAWWIAFSPFVGLFLARISRGRTVREFVLGAVFAPALVCFAWMTILGGTAIDLEISGAAEGAIIAA
SNSAKLFVTLEQMLSGAVLQALIIMCVVLIMTFLVTSADSGILVMNTIMSGGAEETGVKHRIIWGIILTLVIGTLLLAGG
GGLSALQNAMIIGALPFTMIMVLMVLSLIKALFNDGRRERAGVRPAAAQPAE

Sequences:

>Translated_612_residues
MTVKPPFTDLEINTQDSGFYEGYSLPIALVSKIGMVLLVLWALVFPAGAGAILAEVNGTLLNSFNTFYILSVGAFAFFLF
LVAIIPATGKRVLGMSGQKPEFSNFSWFSMMFGAGLGVGLMTFATAEPLGLWGSNPSVVSREVAGNTPEALQSTFRYTFL
HYGFHAWAIYVVTGLSLAYYAYTRGMPLTIRSALTPLIGRFANGAFGHLIDVLGVVATILGVSVTIGFGVSQLVDGVFSI
TGAEWMMDTTGDTPVATKTGLIAALVVIMGMSIISAVSGVGRGVKYLSNLNLVLSLILLMTFVIFGSFFFAMTTYATALI
DYLLHFVNLSFEAYAPLAQDAFATAAPASVQALPAEELAAVYAGATNQWGSYAGFTAGLEGAAAALPAADLDAAYAAGEQ
GRLFGWQAGWTTFYWAWWIAFSPFVGLFLARISRGRTVREFVLGAVFAPALVCFAWMTILGGTAIDLEISGAAEGAIIAA
SNSAKLFVTLEQMLSGAVLQALIIMCVVLIMTFLVTSADSGILVMNTIMSGGAEETGVKHRIIWGIILTLVIGTLLLAGG
GGLSALQNAMIIGALPFTMIMVLMVLSLIKALFNDGRRERAGVRPAAAQPAE
>Mature_611_residues
TVKPPFTDLEINTQDSGFYEGYSLPIALVSKIGMVLLVLWALVFPAGAGAILAEVNGTLLNSFNTFYILSVGAFAFFLFL
VAIIPATGKRVLGMSGQKPEFSNFSWFSMMFGAGLGVGLMTFATAEPLGLWGSNPSVVSREVAGNTPEALQSTFRYTFLH
YGFHAWAIYVVTGLSLAYYAYTRGMPLTIRSALTPLIGRFANGAFGHLIDVLGVVATILGVSVTIGFGVSQLVDGVFSIT
GAEWMMDTTGDTPVATKTGLIAALVVIMGMSIISAVSGVGRGVKYLSNLNLVLSLILLMTFVIFGSFFFAMTTYATALID
YLLHFVNLSFEAYAPLAQDAFATAAPASVQALPAEELAAVYAGATNQWGSYAGFTAGLEGAAAALPAADLDAAYAAGEQG
RLFGWQAGWTTFYWAWWIAFSPFVGLFLARISRGRTVREFVLGAVFAPALVCFAWMTILGGTAIDLEISGAAEGAIIAAS
NSAKLFVTLEQMLSGAVLQALIIMCVVLIMTFLVTSADSGILVMNTIMSGGAEETGVKHRIIWGIILTLVIGTLLLAGGG
GLSALQNAMIIGALPFTMIMVLMVLSLIKALFNDGRRERAGVRPAAAQPAE

Specific function: High-Affinity Uptake Of Choline Driven By A Proton- Motive Force. [C]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=592, Percent_Identity=29.8986486486487, Blast_Score=219, Evalue=6e-58,
Organism=Escherichia coli, GI1786224, Length=195, Percent_Identity=34.3589743589744, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788102, Length=193, Percent_Identity=25.3886010362694, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 64582; Mature: 64451

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKPPFTDLEINTQDSGFYEGYSLPIALVSKIGMVLLVLWALVFPAGAGAILAEVNGTL
CCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH
LNSFNTFYILSVGAFAFFLFLVAIIPATGKRVLGMSGQKPEFSNFSWFSMMFGAGLGVGL
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCHHHH
MTFATAEPLGLWGSNPSVVSREVAGNTPEALQSTFRYTFLHYGFHAWAIYVVTGLSLAYY
HHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYTRGMPLTIRSALTPLIGRFANGAFGHLIDVLGVVATILGVSVTIGFGVSQLVDGVFSI
HHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TGAEWMMDTTGDTPVATKTGLIAALVVIMGMSIISAVSGVGRGVKYLSNLNLVLSLILLM
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFVIFGSFFFAMTTYATALIDYLLHFVNLSFEAYAPLAQDAFATAAPASVQALPAEELAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCHHHCCCHHHHHH
VYAGATNQWGSYAGFTAGLEGAAAALPAADLDAAYAAGEQGRLFGWQAGWTTFYWAWWIA
HHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
FSPFVGLFLARISRGRTVREFVLGAVFAPALVCFAWMTILGGTAIDLEISGAAEGAIIAA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEE
SNSAKLFVTLEQMLSGAVLQALIIMCVVLIMTFLVTSADSGILVMNTIMSGGAEETGVKH
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCHH
RIIWGIILTLVIGTLLLAGGGGLSALQNAMIIGALPFTMIMVLMVLSLIKALFNDGRRER
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AGVRPAAAQPAE
CCCCCCCCCCCC
>Mature Secondary Structure 
TVKPPFTDLEINTQDSGFYEGYSLPIALVSKIGMVLLVLWALVFPAGAGAILAEVNGTL
CCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHH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CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]