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The map label for this gene is rne [H]

Identifier: 110679532

GI number: 110679532

Start: 2168060

End: 2170819

Strand: Direct

Name: rne [H]

Synonym: RD1_2267

Alternate gene names: 110679532

Gene position: 2168060-2170819 (Clockwise)

Preceding gene: 110679531

Following gene: 110679534

Centisome position: 52.46

GC content: 60.29

Gene sequence:

>2760_bases
ATGGCTAAGAAAATGCTTATCGATGCCACCCACGCGGAAGAAACCCGCGTTGTGGTGGTGGATGGAAACAAGGTTGAAGA
GTTCGATTTTGAATCCGAAAACAAACGCCAGCTTGCTGGCAACATCTATCTTGCAAAAGTAACACGGGTTGAGCCGTCCT
TGCAGGCGGCCTTTGTGGAGTACGGGGGGAACCGTCATGGTTTCCTCGCCTTTTCAGAAATTCATCCGGATTATTACCAG
ATTCCCGTCGCGGACCGTCAGGCCCTGATGGAAGAAGAGCGCGCCTATGCCGAAGCGCAAAAGGCCAAGGAAGACGAGGA
CGAAAAGCCCAAACGTCGCAGATCGCGCAGCCGCACAAAGGCCAAGGGCGAAGACACCACGTCAGAGGATGTGGTCGCGA
CGAAGGATGTGGAGTCCGACCAGATCGACGGCATGGAAACCATTGATCTGGATGACAGCGAAGAGGGGTCCTCTCCGATG
GAGCGGGTCGCCGAGACACCTGTCGAAGAGCCCGAGGGCGATGATGCAAGCGCGGAGGCTGCGCAGACGGCTGACGCGTC
CGATGATCAGGACGCGCAAGCAGCGTCTGAGGATGCCTCGGAAGACGATACCGCGACTGAAGATGGCGACGATGATGACG
ACGACGACGATGATGCGCCGCGCAAATCTGATGCATCCTCCAAGGATGACACCATCGAATCCGTCGCGGATGACGATGAT
CAAGACGATATCCGCCCCGTGCGCAAACCCCGGCCACGGCGCTATAAAATTCAGGAAGTCATCAAGGTCCGCCAGATTCT
GCTCGTGCAGGTCGTCAAGGAAGAGCGGGGCAACAAGGGCGCGGCGCTGACCACATACCTCAGCCTTGCAGGGCGCTATT
GCGTCCTGATGCCCAACACGGCGCGCGGCGGTGGTATTTCGCGCAAGATCACGAATGCTGCGGACCGCAAGAAGCTCAAG
GAGATCGCAAATGAGATCGAAGTGCCGCAAGGCGCGGGTCTCATCGTGCGCACCGCCGGGGCCAAACGCACCAAGGCCGA
GATCAAACGCGACTACGAATATCTGCAACGCCTGTGGGAGCAGATCCGCGAGTTGACGCTGAAATCCGTCGCGCCCGCCA
AGATCTACGAAGAGGGAGACCTGATCAAACGCTCGATCCGCGATCTCTACAACCGCGAGATTGACGAGGTGTTTGTCGAA
GGTGAACGGGGCTACCGCATCGCCAAGGACTTCATGAAGATGATCATGCCGTCCCATGCAAAAAACGTGAAACTCTATAC
CGAAGGTCTGCCGCTTTTCGCCCGCTACCAGGTCGAAAGCTATCTTGCGTCGATGTTCAACCCGACTGTGCAGCTTCCGT
CGGGGGGGTATATCGTGATCGGCGTGACCGAGGCTCTGGTGGCGATTGATGTGAACTCCGGGCGGGCCACCAAGGAGGGC
TCGATCGAGCAGACCGCGCTCAAGACCAACCTTGAGGCCGCCGACGAAGTGGCGCGGCAGTTGCGCCTGCGGGACCTCGC
GGGCCTGATCGTGATCGACTTCATCGACATGGATGAGCGCAAGAACAACACCTCCGTCGAAAAGCGCATGAAAGACAAGC
TCAAGACGGACCGCGCGCGCATTCAAGTGGGGCGTATCTCCGGCTTTGGTCTCATGGAAATGTCGCGCCAGCGCCTGCGC
CCCGGCATGATCGAGGCAACGACACAGCCGTGTCAGGCCTGCCATGGTACGGGCCTGATCCGGTCCGATGATAGCCTCGC
ATTGTCCATCCTGCGCCAGATCGAGGAAGAGGGCACGCGCAAGCGGTCCCGAGAGGTGTTGATCAAGGCGCCGGTTGGTA
TTGCCAATTTCCTCATGAACCAGAAGCGCGAACATATTGCGCATATCGAAGCGCGCTACGGGCTGTCTGTGCGGATCGAA
GGCGACCCCATGCTGGTCAGCCCCGATTTCAGTCTTGAGAAGTTCAAGACGGCCACACGGGTCGTGTCGGTTGCAGCACA
ACATGTTGTTTCTGTTGATACCTCATTGATGGATCAGGTGGATGCGGACGACGATGAGGCGCCAGCGGAAACGGCCGATA
ACGGCGCAGAGACGGCAAACAAGGAAAACAACGTCGAGAATGATGGCGAGGATCGGCCCAAGCGCCGTCGCCGGCGTCGT
CGGCGTCGCAACAAGAACGGCAATGGCGAGACGCAGACCGCATCCGATGAGACAAATGCCGAGGCATCCGAGGCCCAGGG
CGCTAAGACTCCGAAGAAAGCGGAGCCTGCAGCCGACAGCCCGGTAGAGGCGCCTGCTGCGCAAACCGATGATGCGCCAG
CCCCTGCGGCAGAGCAGGAAGAGGCACCGGCAAAACCCAAACGGACACGGTCGCGCACGCGCAAACCGAAAGTGGAAGCC
GACACACAGGATGCGCCAGAAGCAGAGGCGAAAGCCGAAACCGCCGCGCCAGTGGAAGAAACCGCACCCGCCGAAGCGAC
CGAAGAAACCCCTGCAAAACCAAAGCGTAAACGGGCCAGCCGGGCCAAGAAGGCCGCTCCGGTTGAGGCGGCACCAGACG
CCGAACCAGCCCCCGTGGCGCAGGACGCCGAGCCGGCACCTTCATCACAGGAAGCTGCGGCGGCACCGGTGGCAGAAGAG
CCAGCGCCAGCAGCGCCTGAACCGGAACCTGCAGGTGCCGAGCCCGAACCGGTGGTCGCTGAGGCCGCGCCAGAGCCAGC
CAAACCAAAGCGGCGCGGGTGGTGGTCCATCGGAGGCTAA

Upstream 100 bases:

>100_bases
CCCGGCCATGCAGTGCGTCTCAGATCGCCCTGACAAGACATACCCCCCACTGCAACGCTCCCCCGGGGGCTGTGCGGTCG
CGGCTATGCAATTTGGAAAT

Downstream 100 bases:

>100_bases
GGGCTTTCCAACACCAAAAACCCAAAAAGCGCGTCCAAATCGGGCGCGCTTTTTCATGTCACCTCATTTTTTGCGGATGA
CATAGACCTGCACCGGCCCA

Product: ribonuclease E, putative

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:

>919_residues
MAKKMLIDATHAEETRVVVVDGNKVEEFDFESENKRQLAGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRQALMEEERAYAEAQKAKEDEDEKPKRRRSRSRTKAKGEDTTSEDVVATKDVESDQIDGMETIDLDDSEEGSSPM
ERVAETPVEEPEGDDASAEAAQTADASDDQDAQAASEDASEDDTATEDGDDDDDDDDDAPRKSDASSKDDTIESVADDDD
QDDIRPVRKPRPRRYKIQEVIKVRQILLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLK
EIANEIEVPQGAGLIVRTAGAKRTKAEIKRDYEYLQRLWEQIRELTLKSVAPAKIYEEGDLIKRSIRDLYNREIDEVFVE
GERGYRIAKDFMKMIMPSHAKNVKLYTEGLPLFARYQVESYLASMFNPTVQLPSGGYIVIGVTEALVAIDVNSGRATKEG
SIEQTALKTNLEAADEVARQLRLRDLAGLIVIDFIDMDERKNNTSVEKRMKDKLKTDRARIQVGRISGFGLMEMSRQRLR
PGMIEATTQPCQACHGTGLIRSDDSLALSILRQIEEEGTRKRSREVLIKAPVGIANFLMNQKREHIAHIEARYGLSVRIE
GDPMLVSPDFSLEKFKTATRVVSVAAQHVVSVDTSLMDQVDADDDEAPAETADNGAETANKENNVENDGEDRPKRRRRRR
RRRNKNGNGETQTASDETNAEASEAQGAKTPKKAEPAADSPVEAPAAQTDDAPAPAAEQEEAPAKPKRTRSRTRKPKVEA
DTQDAPEAEAKAETAAPVEETAPAEATEETPAKPKRKRASRAKKAAPVEAAPDAEPAPVAQDAEPAPSSQEAAAAPVAEE
PAPAAPEPEPAGAEPEPVVAEAAPEPAKPKRRGWWSIGG

Sequences:

>Translated_919_residues
MAKKMLIDATHAEETRVVVVDGNKVEEFDFESENKRQLAGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRQALMEEERAYAEAQKAKEDEDEKPKRRRSRSRTKAKGEDTTSEDVVATKDVESDQIDGMETIDLDDSEEGSSPM
ERVAETPVEEPEGDDASAEAAQTADASDDQDAQAASEDASEDDTATEDGDDDDDDDDDAPRKSDASSKDDTIESVADDDD
QDDIRPVRKPRPRRYKIQEVIKVRQILLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLK
EIANEIEVPQGAGLIVRTAGAKRTKAEIKRDYEYLQRLWEQIRELTLKSVAPAKIYEEGDLIKRSIRDLYNREIDEVFVE
GERGYRIAKDFMKMIMPSHAKNVKLYTEGLPLFARYQVESYLASMFNPTVQLPSGGYIVIGVTEALVAIDVNSGRATKEG
SIEQTALKTNLEAADEVARQLRLRDLAGLIVIDFIDMDERKNNTSVEKRMKDKLKTDRARIQVGRISGFGLMEMSRQRLR
PGMIEATTQPCQACHGTGLIRSDDSLALSILRQIEEEGTRKRSREVLIKAPVGIANFLMNQKREHIAHIEARYGLSVRIE
GDPMLVSPDFSLEKFKTATRVVSVAAQHVVSVDTSLMDQVDADDDEAPAETADNGAETANKENNVENDGEDRPKRRRRRR
RRRNKNGNGETQTASDETNAEASEAQGAKTPKKAEPAADSPVEAPAAQTDDAPAPAAEQEEAPAKPKRTRSRTRKPKVEA
DTQDAPEAEAKAETAAPVEETAPAEATEETPAKPKRKRASRAKKAAPVEAAPDAEPAPVAQDAEPAPSSQEAAAAPVAEE
PAPAAPEPEPAGAEPEPVVAEAAPEPAKPKRRGWWSIGG
>Mature_918_residues
AKKMLIDATHAEETRVVVVDGNKVEEFDFESENKRQLAGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQI
PVADRQALMEEERAYAEAQKAKEDEDEKPKRRRSRSRTKAKGEDTTSEDVVATKDVESDQIDGMETIDLDDSEEGSSPME
RVAETPVEEPEGDDASAEAAQTADASDDQDAQAASEDASEDDTATEDGDDDDDDDDDAPRKSDASSKDDTIESVADDDDQ
DDIRPVRKPRPRRYKIQEVIKVRQILLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNTARGGGISRKITNAADRKKLKE
IANEIEVPQGAGLIVRTAGAKRTKAEIKRDYEYLQRLWEQIRELTLKSVAPAKIYEEGDLIKRSIRDLYNREIDEVFVEG
ERGYRIAKDFMKMIMPSHAKNVKLYTEGLPLFARYQVESYLASMFNPTVQLPSGGYIVIGVTEALVAIDVNSGRATKEGS
IEQTALKTNLEAADEVARQLRLRDLAGLIVIDFIDMDERKNNTSVEKRMKDKLKTDRARIQVGRISGFGLMEMSRQRLRP
GMIEATTQPCQACHGTGLIRSDDSLALSILRQIEEEGTRKRSREVLIKAPVGIANFLMNQKREHIAHIEARYGLSVRIEG
DPMLVSPDFSLEKFKTATRVVSVAAQHVVSVDTSLMDQVDADDDEAPAETADNGAETANKENNVENDGEDRPKRRRRRRR
RRNKNGNGETQTASDETNAEASEAQGAKTPKKAEPAADSPVEAPAAQTDDAPAPAAEQEEAPAKPKRTRSRTRKPKVEAD
TQDAPEAEAKAETAAPVEETAPAEATEETPAKPKRKRASRAKKAAPVEAAPDAEPAPVAQDAEPAPSSQEAAAAPVAEEP
APAAPEPEPAGAEPEPVVAEAAPEPAKPKRRGWWSIGG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=405, Percent_Identity=46.9135802469136, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI87082235, Length=394, Percent_Identity=32.741116751269, Blast_Score=199, Evalue=9e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 101034; Mature: 100903

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKMLIDATHAEETRVVVVDGNKVEEFDFESENKRQLAGNIYLAKVTRVEPSLQAAFVE
CCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRQALMEEERAYAEAQKAKEDEDEKPKRRRSRSRTK
HCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AKGEDTTSEDVVATKDVESDQIDGMETIDLDDSEEGSSPMERVAETPVEEPEGDDASAEA
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHH
AQTADASDDQDAQAASEDASEDDTATEDGDDDDDDDDDAPRKSDASSKDDTIESVADDDD
HHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
QDDIRPVRKPRPRRYKIQEVIKVRQILLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNT
CHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCC
ARGGGISRKITNAADRKKLKEIANEIEVPQGAGLIVRTAGAKRTKAEIKRDYEYLQRLWE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QIRELTLKSVAPAKIYEEGDLIKRSIRDLYNREIDEVFVEGERGYRIAKDFMKMIMPSHA
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCC
KNVKLYTEGLPLFARYQVESYLASMFNPTVQLPSGGYIVIGVTEALVAIDVNSGRATKEG
CCEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCEEEEEECCCCCCCCCC
SIEQTALKTNLEAADEVARQLRLRDLAGLIVIDFIDMDERKNNTSVEKRMKDKLKTDRAR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
IQVGRISGFGLMEMSRQRLRPGMIEATTQPCQACHGTGLIRSDDSLALSILRQIEEEGTR
EEEECCCCCCHHHHHHHHCCCCCHHHCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
KRSREVLIKAPVGIANFLMNQKREHIAHIEARYGLSVRIEGDPMLVSPDFSLEKFKTATR
HHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCHHHHHHHHH
VVSVAAQHVVSVDTSLMDQVDADDDEAPAETADNGAETANKENNVENDGEDRPKRRRRRR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
RRRNKNGNGETQTASDETNAEASEAQGAKTPKKAEPAADSPVEAPAAQTDDAPAPAAEQE
HHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAPAKPKRTRSRTRKPKVEADTQDAPEAEAKAETAAPVEETAPAEATEETPAKPKRKRAS
CCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHH
RAKKAAPVEAAPDAEPAPVAQDAEPAPSSQEAAAAPVAEEPAPAAPEPEPAGAEPEPVVA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEC
EAAPEPAKPKRRGWWSIGG
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKMLIDATHAEETRVVVVDGNKVEEFDFESENKRQLAGNIYLAKVTRVEPSLQAAFVE
CCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCEEEEEEECCCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRQALMEEERAYAEAQKAKEDEDEKPKRRRSRSRTK
HCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AKGEDTTSEDVVATKDVESDQIDGMETIDLDDSEEGSSPMERVAETPVEEPEGDDASAEA
CCCCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHH
AQTADASDDQDAQAASEDASEDDTATEDGDDDDDDDDDAPRKSDASSKDDTIESVADDDD
HHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
QDDIRPVRKPRPRRYKIQEVIKVRQILLVQVVKEERGNKGAALTTYLSLAGRYCVLMPNT
CHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCC
ARGGGISRKITNAADRKKLKEIANEIEVPQGAGLIVRTAGAKRTKAEIKRDYEYLQRLWE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
QIRELTLKSVAPAKIYEEGDLIKRSIRDLYNREIDEVFVEGERGYRIAKDFMKMIMPSHA
HHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHCCCCC
KNVKLYTEGLPLFARYQVESYLASMFNPTVQLPSGGYIVIGVTEALVAIDVNSGRATKEG
CCEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCEEEEEECCCCCCCCCC
SIEQTALKTNLEAADEVARQLRLRDLAGLIVIDFIDMDERKNNTSVEKRMKDKLKTDRAR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
IQVGRISGFGLMEMSRQRLRPGMIEATTQPCQACHGTGLIRSDDSLALSILRQIEEEGTR
EEEECCCCCCHHHHHHHHCCCCCHHHCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
KRSREVLIKAPVGIANFLMNQKREHIAHIEARYGLSVRIEGDPMLVSPDFSLEKFKTATR
HHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECCCCCHHHHHHHHH
VVSVAAQHVVSVDTSLMDQVDADDDEAPAETADNGAETANKENNVENDGEDRPKRRRRRR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
RRRNKNGNGETQTASDETNAEASEAQGAKTPKKAEPAADSPVEAPAAQTDDAPAPAAEQE
HHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EAPAKPKRTRSRTRKPKVEADTQDAPEAEAKAETAAPVEETAPAEATEETPAKPKRKRAS
CCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHH
RAKKAAPVEAAPDAEPAPVAQDAEPAPSSQEAAAAPVAEEPAPAAPEPEPAGAEPEPVVA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEC
EAAPEPAKPKRRGWWSIGG
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]