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The map label for this gene is rhtB [H]

Identifier: 110679423

GI number: 110679423

Start: 2045712

End: 2046332

Strand: Reverse

Name: rhtB [H]

Synonym: RD1_2149

Alternate gene names: 110679423

Gene position: 2046332-2045712 (Counterclockwise)

Preceding gene: 110679424

Following gene: 110679422

Centisome position: 49.51

GC content: 62.32

Gene sequence:

>621_bases
ATGCCTCTCGACCTCTGGCTGACCTTTGTTGCAGCCTCCACCGCGCTCCTGCTGATCCCTGGCCCGACTGTGCTTTTGGT
GCTCAGCTATGCGCTGTCCAAAGGGCGCTCGGTCGCCGTGGCCTCGGCAGCCGGCGTTGCTTTCGGCGACCTTATTGCGA
TGACTGCATCTTTGCTGGGCCTTGGCGCGCTCCTGCTGGCCTCTGCGACGGCCTTTACGGTTCTCAAATGGGTCGGTGCC
GCCTATCTGGTCTGGCTCGGCATCAAGCTGATCCGCTCTGCCCCCACGGACGGTTTGACCCCTGCACGCGATGTGACCGC
GAATGGTGTCTTTGGTCACACGGCCATGGTGACAGCGCTCAACCCGAAATCCATCGCCTTTTTCATCGCCTTTGTACCGC
AGTTCATCCGGCCTGACGCAGCACTCTTGCCGCAATTCGCGATCCTGATCGCAACTTTTGTCACGCTTGCTGCCCTGAAC
GCACTGGCCTATGCGCTTCTGGCGGATCGTTTGCGCCGTGTCATCGCCAGACCCTCCGTCCTGACGTGGATCACCCGCGC
AGGCGGCGCCACCCTGATTGGCATGGGCGTGCTGACCGCAACCCTGCGCCGCGCCACATAA

Upstream 100 bases:

>100_bases
ATTTCGCGATCCTGACGCAACCCGACGCACGCGATCCGGCGGTCATGGCCAAGCTGGATGCCGTGGCCGGGCGCGTACTC
TAATCCAAGGAATCCATGAA

Downstream 100 bases:

>100_bases
CCCTGAGAGACATTATGAAAACCGTACAAGACTACATCCGCACCATCGTCGATTTCCCCCACGAGGGGATCATGTTTCGC
GATGTGACCACGCTTTTCGC

Product: LysE family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MPLDLWLTFVAASTALLLIPGPTVLLVLSYALSKGRSVAVASAAGVAFGDLIAMTASLLGLGALLLASATAFTVLKWVGA
AYLVWLGIKLIRSAPTDGLTPARDVTANGVFGHTAMVTALNPKSIAFFIAFVPQFIRPDAALLPQFAILIATFVTLAALN
ALAYALLADRLRRVIARPSVLTWITRAGGATLIGMGVLTATLRRAT

Sequences:

>Translated_206_residues
MPLDLWLTFVAASTALLLIPGPTVLLVLSYALSKGRSVAVASAAGVAFGDLIAMTASLLGLGALLLASATAFTVLKWVGA
AYLVWLGIKLIRSAPTDGLTPARDVTANGVFGHTAMVTALNPKSIAFFIAFVPQFIRPDAALLPQFAILIATFVTLAALN
ALAYALLADRLRRVIARPSVLTWITRAGGATLIGMGVLTATLRRAT
>Mature_205_residues
PLDLWLTFVAASTALLLIPGPTVLLVLSYALSKGRSVAVASAAGVAFGDLIAMTASLLGLGALLLASATAFTVLKWVGAA
YLVWLGIKLIRSAPTDGLTPARDVTANGVFGHTAMVTALNPKSIAFFIAFVPQFIRPDAALLPQFAILIATFVTLAALNA
LAYALLADRLRRVIARPSVLTWITRAGGATLIGMGVLTATLRRAT

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=35.4368932038835, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788099, Length=140, Percent_Identity=30.7142857142857, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21414; Mature: 21283

Theoretical pI: Translated: 11.70; Mature: 11.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDLWLTFVAASTALLLIPGPTVLLVLSYALSKGRSVAVASAAGVAFGDLIAMTASLLG
CCHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
LGALLLASATAFTVLKWVGAAYLVWLGIKLIRSAPTDGLTPARDVTANGVFGHTAMVTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEE
NPKSIAFFIAFVPQFIRPDAALLPQFAILIATFVTLAALNALAYALLADRLRRVIARPSV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTWITRAGGATLIGMGVLTATLRRAT
HHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLDLWLTFVAASTALLLIPGPTVLLVLSYALSKGRSVAVASAAGVAFGDLIAMTASLLG
CHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
LGALLLASATAFTVLKWVGAAYLVWLGIKLIRSAPTDGLTPARDVTANGVFGHTAMVTAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHEEEEE
NPKSIAFFIAFVPQFIRPDAALLPQFAILIATFVTLAALNALAYALLADRLRRVIARPSV
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTWITRAGGATLIGMGVLTATLRRAT
HHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]