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The map label for this gene is gspE [C]

Identifier: 110679393

GI number: 110679393

Start: 2018544

End: 2020001

Strand: Reverse

Name: gspE [C]

Synonym: RD1_2116

Alternate gene names: 110679393

Gene position: 2020001-2018544 (Counterclockwise)

Preceding gene: 110679394

Following gene: 110679392

Centisome position: 48.87

GC content: 59.88

Gene sequence:

>1458_bases
ATGTTCTCGAAGTACAAGAAGGCAAAGGGCGCGACACCCGTCGCGTCAGGCGCTCTGGCCCAGCAAGCTGGATCCGGCGA
TCCTGCCAATGCGCCGGTGCAAAATGTCATGCGCAAGGCGGCGCAACCCGCCCCGGCACAGGCCGCGGCCATGGACAAGG
AACGCAAACGCAAGGAGCGGATGGGCGAGATCAAGCTGGACCTGCATCACGTGCTGCTCGACAACCTCAATCTTGCGGCA
CTTGAACATGCGACCGAACAGGATTTGCGTCAGGAAATCAGCGCCATCACCAGCGAGCATCTGCAAGCCCAGAGCATCGT
TCTCAACCGCGAAGACCGCCAGACGCTGACATCCGAACTCTATGATGAGGTCACAGGGCTCGGTCCGCTTGAGACGCTTT
TGAAAGACGACTCGGTCAACGATATTTTGGTCAACGGGCCGCAGCAGATATTCGTGGAACGGGATGGCAAACTGCAACTC
ACGGATGTGATGTTCAAGGATGAACGCCATCTGCTACGCATTATCGACAAGATCGTCTCGGCCGTCGGGCGGCGCGTCGA
TGAAAGCAACCCCTACGTCGATGCCCGCCTCAAGGACGGGTCGCGGTTTAACGCCATGGTGCCCCCCGTGGCCGTGGACG
GCAGCCTCGTGTCGATCCGGAAATTCAAGAAAGACAAGCTGGGCATTGATGATCTGGTGCATTTCGGCGCCTTTACCGAA
GAGATGGCCGCCTATCTGCAGGCGGCTGTGGCGACGCGCCTCAACATCATTGTTTCGGGCGGGACAGGCTCGGGCAAGAC
CACAACCCTGAACGCCCTGTCGTCCTTCATCGACAACTCGGAGCGTATCCTGACCATCGAGGACACGGCGGAACTGCAAT
TGCAGCAGACACACGTGGGCCGCATGGAAAGCCGACCGCCCAACGTGGAGGGCAAGGGCGAAGTTTCCCCCCGTGACTGC
CTCAAGAACGCGCTGCGGATGCGGCCTGATCGCATCATCGTGGGCGAGACGCGTGGCGAGGAAGTGATTGACATGCTGCA
GGCGATGAACACGGGTCACGATGGATCAATGACGACAATCCACGCGAATTCGGCGCGCGACGGGGTGTCTCGTCTGGAAA
ACATGATCGCCATGGCGGGGATTGAAATGCCGCTCAAGGCGGTGCGCAGTCAGATCGCCTCGGCGGTGAACCTGATTGTT
CAGGCCTCGCGCCTGCAGGATGGCTCACGCCGCATGACCTCGATCACCGAGGTCACGGGGATGGAGGGTGAAGTCATCTC
GATGCAGGAGATTTTCCGCTACCAGCGCGTGGGTCTCACACCGGAAAACAAGATCATCGGCCACTACACGGCCACGGGTG
TGCGCAGCGCCTATGCCGAACGCTTCCGTATGTGGGGCTACGATTTGCCGGCTTCCATCTATGAACCCACCGCAGCCCCG
CAAGGGTTTTCAACGTGA

Upstream 100 bases:

>100_bases
AAAAACCCATTACGTCATGAGATCGCCAAGCTCGCAGCCTCCATCCTCGCGCTTGGTACGCGCGACGTCAAAGCCGCCTG
ATTTTCAAAGGAAACCCTGC

Downstream 100 bases:

>100_bases
CCTGCCGTTGCGTCACTTGAGGATTTGACCATGAGCCCAGAACCCATCATCTATGGCCTGATCTTTATCGGCGTGCTTGT
TCTGGTCGAGGGTCTTTACC

Product: type II/IV secretion system protein, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MFSKYKKAKGATPVASGALAQQAGSGDPANAPVQNVMRKAAQPAPAQAAAMDKERKRKERMGEIKLDLHHVLLDNLNLAA
LEHATEQDLRQEISAITSEHLQAQSIVLNREDRQTLTSELYDEVTGLGPLETLLKDDSVNDILVNGPQQIFVERDGKLQL
TDVMFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKKDKLGIDDLVHFGAFTE
EMAAYLQAAVATRLNIIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSPRDC
LKNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANSARDGVSRLENMIAMAGIEMPLKAVRSQIASAVNLIV
QASRLQDGSRRMTSITEVTGMEGEVISMQEIFRYQRVGLTPENKIIGHYTATGVRSAYAERFRMWGYDLPASIYEPTAAP
QGFST

Sequences:

>Translated_485_residues
MFSKYKKAKGATPVASGALAQQAGSGDPANAPVQNVMRKAAQPAPAQAAAMDKERKRKERMGEIKLDLHHVLLDNLNLAA
LEHATEQDLRQEISAITSEHLQAQSIVLNREDRQTLTSELYDEVTGLGPLETLLKDDSVNDILVNGPQQIFVERDGKLQL
TDVMFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKKDKLGIDDLVHFGAFTE
EMAAYLQAAVATRLNIIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSPRDC
LKNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANSARDGVSRLENMIAMAGIEMPLKAVRSQIASAVNLIV
QASRLQDGSRRMTSITEVTGMEGEVISMQEIFRYQRVGLTPENKIIGHYTATGVRSAYAERFRMWGYDLPASIYEPTAAP
QGFST
>Mature_485_residues
MFSKYKKAKGATPVASGALAQQAGSGDPANAPVQNVMRKAAQPAPAQAAAMDKERKRKERMGEIKLDLHHVLLDNLNLAA
LEHATEQDLRQEISAITSEHLQAQSIVLNREDRQTLTSELYDEVTGLGPLETLLKDDSVNDILVNGPQQIFVERDGKLQL
TDVMFKDERHLLRIIDKIVSAVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKKDKLGIDDLVHFGAFTE
EMAAYLQAAVATRLNIIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVGRMESRPPNVEGKGEVSPRDC
LKNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTIHANSARDGVSRLENMIAMAGIEMPLKAVRSQIASAVNLIV
QASRLQDGSRRMTSITEVTGMEGEVISMQEIFRYQRVGLTPENKIIGHYTATGVRSAYAERFRMWGYDLPASIYEPTAAP
QGFST

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=189, Percent_Identity=31.7460317460317, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53250; Mature: 53250

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKYKKAKGATPVASGALAQQAGSGDPANAPVQNVMRKAAQPAPAQAAAMDKERKRKER
CCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGEIKLDLHHVLLDNLNLAALEHATEQDLRQEISAITSEHLQAQSIVLNREDRQTLTSEL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YDEVTGLGPLETLLKDDSVNDILVNGPQQIFVERDGKLQLTDVMFKDERHLLRIIDKIVS
HHHHHCCCHHHHHHCCCCCCCEEECCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHH
AVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKKDKLGIDDLVHFGAFTE
HHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
EMAAYLQAAVATRLNIIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVG
HHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHC
RMESRPPNVEGKGEVSPRDCLKNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTI
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEE
HANSARDGVSRLENMIAMAGIEMPLKAVRSQIASAVNLIVQASRLQDGSRRMTSITEVTG
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
MEGEVISMQEIFRYQRVGLTPENKIIGHYTATGVRSAYAERFRMWGYDLPASIYEPTAAP
CCCCEECHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
QGFST
CCCCC
>Mature Secondary Structure
MFSKYKKAKGATPVASGALAQQAGSGDPANAPVQNVMRKAAQPAPAQAAAMDKERKRKER
CCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MGEIKLDLHHVLLDNLNLAALEHATEQDLRQEISAITSEHLQAQSIVLNREDRQTLTSEL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YDEVTGLGPLETLLKDDSVNDILVNGPQQIFVERDGKLQLTDVMFKDERHLLRIIDKIVS
HHHHHCCCHHHHHHCCCCCCCEEECCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHH
AVGRRVDESNPYVDARLKDGSRFNAMVPPVAVDGSLVSIRKFKKDKLGIDDLVHFGAFTE
HHHHHCCCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHH
EMAAYLQAAVATRLNIIVSGGTGSGKTTTLNALSSFIDNSERILTIEDTAELQLQQTHVG
HHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHC
RMESRPPNVEGKGEVSPRDCLKNALRMRPDRIIVGETRGEEVIDMLQAMNTGHDGSMTTI
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEE
HANSARDGVSRLENMIAMAGIEMPLKAVRSQIASAVNLIVQASRLQDGSRRMTSITEVTG
ECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
MEGEVISMQEIFRYQRVGLTPENKIIGHYTATGVRSAYAERFRMWGYDLPASIYEPTAAP
CCCCEECHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
QGFST
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]