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The map label for this gene is tolB

Identifier: 110679376

GI number: 110679376

Start: 2000521

End: 2001852

Strand: Direct

Name: tolB

Synonym: RD1_2097

Alternate gene names: 110679376

Gene position: 2000521-2001852 (Clockwise)

Preceding gene: 110679375

Following gene: 110679377

Centisome position: 48.4

GC content: 60.74

Gene sequence:

>1332_bases
ATGAGTTTTCAGATACGCGTTTTCACAGCAATTCTGGCTGTTCTATCGCTCTTCACCGCGCCGGTGCTGGCCCAGAACAG
CGGCCCGCTGCGCATTGAGATCACCGAAGGTGTGATCGAACCTTTGCCCTTTGCCCTGCCGGTTTTCGAGGCCGAGACTG
CCGATGCCGCCGATGTGGCGGCCCAGATCACACAGGTGATCGCGGCGGATCTGACAGGGACGGGACTGTTTCGCCAGATT
GAGCCGGACGCTTTCATCAGCACGGTCAGTAGCTTTGCCGCGCCGATCCAATACGCGGATTGGAAAGCCATCAACGCGCA
AGCGCTGATCACCGGGGCTGTAGCGGTGCAGGGCAATCAGTTGAACGTGAAATTCCGCCTGTATGATGTGTTTTCGGGGG
CGGAAATGGGCGATGGGTTGCAGTTTTCCGCCACGCCCGACGGCTGGCGTCGCATGGCGCATAAGGTGGCGGATGCGGTC
TATAGCCGGATCACGGGGGAGGGCGGGTATTTCGACAGCCGCGTCGTCTACGTCTCCGAAACCGGCACCAAGGATGCACG
CCAGAAGCGGCTGGCGATCATGGATTACGATGGGGCAAATGTGAAATACCTCACCGACAGCAGTTCCATCGTGCTGGCCC
CGCGGTTTTCGCCGGATGGACAGCGCATTCTCTATACCAGCTATGAAACAGGCTTTCCGCGCATCTATGTGCTGGATGTG
ACCTCGCTGCAGCGTCGTGGCCTTGAAAGCCAGGAAGGCACCATGAGCTTTGCGCCGCGTTTTGCACCGGATGGGCAGAC
CATCGTCTTTTCGCTCAGTCAGGGTGGGAATACCGATATCTACCGCATGAACGTCAATGGCGGGTCGGCGACACGGCTCA
CATCGGCGCCCTCGATCGAGACCGCGCCGAGCTATTCGCCCGATGGCACGCAGATCGTGTTCGAAAGCGACAGGTCCGGC
TCTCAACAGCTTTATGTGATGCCCGCAAACGGGGGGGAGGCGCGCCGCATTTCTTTCGGACCCGGACGCTATGGCACGCC
GGTCTGGTCGCCGCGCGGCGATCTCGTCGCCTTCACCAAGCAAAACGCCGGGCGGTTCCATATCGGCGTGATGCGCCTTG
ATGGTTCCGAAGAGCGTTTGCTCACGGCTTCGTTTCTTGACGAAGGGCCCACATGGTCCCCCAATGGCCGCGTGATCATG
TTTTCGCGCGAAACCCAGGGTGCCCAGGGACGCGCCACGCTCTATTCCGTGGACATCACGGGCAGAAACCTGCGCCCGGT
GCGCACGCCCGAAGGAGGGTCGGACCCATCGTGGTCCCCGCTGCAGAGATAA

Upstream 100 bases:

>100_bases
TCATTCGCTGTGGCGCGCGCGGATTCAATTTACCGGTTGAGAAATACAGCCAATGGCAAGAGATTGAGATGACATTCAAC
CCCGAAAGGATGCGGGTCAA

Downstream 100 bases:

>100_bases
CGGTGCGCGCGAAACAATTTCTATTGGCGGCGCGCATCTGGTAAAATATCAGGCATAGAAATATAATCTGACCGAATGAG
GCAAAGAGCATGAAACTTAC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVAAQITQVIAADLTGTGLFRQI
EPDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQLNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAV
YSRITGEGGYFDSRVVYVSETGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDV
TSLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIETAPSYSPDGTQIVFESDRSG
SQQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTKQNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIM
FSRETQGAQGRATLYSVDITGRNLRPVRTPEGGSDPSWSPLQR

Sequences:

>Translated_443_residues
MSFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVAAQITQVIAADLTGTGLFRQI
EPDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQLNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAV
YSRITGEGGYFDSRVVYVSETGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDV
TSLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIETAPSYSPDGTQIVFESDRSG
SQQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTKQNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIM
FSRETQGAQGRATLYSVDITGRNLRPVRTPEGGSDPSWSPLQR
>Mature_442_residues
SFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVAAQITQVIAADLTGTGLFRQIE
PDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQLNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAVY
SRITGEGGYFDSRVVYVSETGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDVT
SLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIETAPSYSPDGTQIVFESDRSGS
QQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTKQNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIMF
SRETQGAQGRATLYSVDITGRNLRPVRTPEGGSDPSWSPLQR

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=416, Percent_Identity=28.8461538461538, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_ROSDO (Q167Z6)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682383.1
- ProteinModelPortal:   Q167Z6
- SMR:   Q167Z6
- STRING:   Q167Z6
- GeneID:   4196644
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2097
- NMPDR:   fig|375451.6.peg.1958
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q167Z6
- ProtClustDB:   PRK05137
- BioCyc:   RDEN375451:RD1_2097-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48084; Mature: 47952

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVA
CCEEEHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHH
AQITQVIAADLTGTGLFRQIEPDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQ
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCEEEEEEEEEEECCE
LNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAVYSRITGEGGYFDSRVVYVSE
EEEEEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
TGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDV
CCCCHHHHCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEC
TSLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIE
HHHHHCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCC
TAPSYSPDGTQIVFESDRSGSQQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
QNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIMFSRETQGAQGRATLYSVDIT
CCCCEEEEEEEEECCCCCEEEEEEHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEC
GRNLRPVRTPEGGSDPSWSPLQR
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SFQIRVFTAILAVLSLFTAPVLAQNSGPLRIEITEGVIEPLPFALPVFEAETADAADVA
CEEEHHHHHHHHHHHHHHCCCEECCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHH
AQITQVIAADLTGTGLFRQIEPDAFISTVSSFAAPIQYADWKAINAQALITGAVAVQGNQ
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCEEEEEEEEEEECCE
LNVKFRLYDVFSGAEMGDGLQFSATPDGWRRMAHKVADAVYSRITGEGGYFDSRVVYVSE
EEEEEEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
TGTKDARQKRLAIMDYDGANVKYLTDSSSIVLAPRFSPDGQRILYTSYETGFPRIYVLDV
CCCCHHHHCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEEC
TSLQRRGLESQEGTMSFAPRFAPDGQTIVFSLSQGGNTDIYRMNVNGGSATRLTSAPSIE
HHHHHCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCC
TAPSYSPDGTQIVFESDRSGSQQLYVMPANGGEARRISFGPGRYGTPVWSPRGDLVAFTK
CCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
QNAGRFHIGVMRLDGSEERLLTASFLDEGPTWSPNGRVIMFSRETQGAQGRATLYSVDIT
CCCCEEEEEEEEECCCCCEEEEEEHHCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEC
GRNLRPVRTPEGGSDPSWSPLQR
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA