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The map label for this gene is ruvC [H]

Identifier: 110679368

GI number: 110679368

Start: 1994802

End: 1995365

Strand: Direct

Name: ruvC [H]

Synonym: RD1_2088

Alternate gene names: 110679368

Gene position: 1994802-1995365 (Clockwise)

Preceding gene: 110679366

Following gene: 110679369

Centisome position: 48.26

GC content: 59.4

Gene sequence:

>564_bases
ATGTTCGCCTTTCGTGCTAATGCGTACAAAACGAATAAAAAGAGCAGGACAGCATCTATGACACGGGTAATCGGCATTGA
TCCGGGGCTGCGCAATCTGGGCTGGGGTGTCATTGAAGTGTCCGGCAGCCGAATCGCGCATATAGCCAATGGGGTTTGCG
TGTCGGGGGGCAGCGATTTGGCAGACCGTTTGCTGTCCCTCCACCAGCAACTGACGGCGGTGGTTTCGACCTTTGCGCCG
GATGAGGCCGCGGTGGAACAGACATTCGTCAACAAGGACGGGGCCGGAACGCTCAAGTTGGGTCAGGCGCGCGGGATTGC
CATGCTGGTGCCCGCGCAGGCCGGTCTGCGCGTAGGTGAATATGCGCCCAACAAGATCAAGAAGACCGTGGTGGGGGTTG
GGCATGCGGATAAGGCGCAGGTGCTGCATATGGTACGCTTGCAATTGCCCGGCGCAGAGATCAAAAGCCCGGATGCGGCG
GATGCTTTGGCGATTGCCATCTGTCATGCACATCACGGGGGGGCGGCTGGTTTGGGCGCGGCAATTGCAAAGGCAACGGC
ATGA

Upstream 100 bases:

>100_bases
CGTGTCGGAATTCTGCCTTTTGATGGCGTTTTCGGGCCTTTGTCGGCGGATGTGCCGGAAAACGCACATAGGGAGATGCG
AAAAAGGAACTGCTTGGCGG

Downstream 100 bases:

>100_bases
TTGGCAAGATCACGGGCCGACTTGAATACCGCGCGAGCGATCATGTGCTGATTGATGTGCGCGGGGTCGGGTATCTGGTC
TTTTGCTCGGAACGTACATT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MFAFRANAYKTNKKSRTASMTRVIGIDPGLRNLGWGVIEVSGSRIAHIANGVCVSGGSDLADRLLSLHQQLTAVVSTFAP
DEAAVEQTFVNKDGAGTLKLGQARGIAMLVPAQAGLRVGEYAPNKIKKTVVGVGHADKAQVLHMVRLQLPGAEIKSPDAA
DALAIAICHAHHGGAAGLGAAIAKATA

Sequences:

>Translated_187_residues
MFAFRANAYKTNKKSRTASMTRVIGIDPGLRNLGWGVIEVSGSRIAHIANGVCVSGGSDLADRLLSLHQQLTAVVSTFAP
DEAAVEQTFVNKDGAGTLKLGQARGIAMLVPAQAGLRVGEYAPNKIKKTVVGVGHADKAQVLHMVRLQLPGAEIKSPDAA
DALAIAICHAHHGGAAGLGAAIAKATA
>Mature_187_residues
MFAFRANAYKTNKKSRTASMTRVIGIDPGLRNLGWGVIEVSGSRIAHIANGVCVSGGSDLADRLLSLHQQLTAVVSTFAP
DEAAVEQTFVNKDGAGTLKLGQARGIAMLVPAQAGLRVGEYAPNKIKKTVVGVGHADKAQVLHMVRLQLPGAEIKSPDAA
DALAIAICHAHHGGAAGLGAAIAKATA

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=133, Percent_Identity=42.8571428571429, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 19235; Mature: 19235

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAFRANAYKTNKKSRTASMTRVIGIDPGLRNLGWGVIEVSGSRIAHIANGVCVSGGSDL
CCEEECCCCCCCCCHHHHHHEEEEECCCCHHHCCCEEEEECCCEEEECCCCEEECCCHHH
ADRLLSLHQQLTAVVSTFAPDEAAVEQTFVNKDGAGTLKLGQARGIAMLVPAQAGLRVGE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCC
YAPNKIKKTVVGVGHADKAQVLHMVRLQLPGAEIKSPDAADALAIAICHAHHGGAAGLGA
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
AIAKATA
HHHHCCC
>Mature Secondary Structure
MFAFRANAYKTNKKSRTASMTRVIGIDPGLRNLGWGVIEVSGSRIAHIANGVCVSGGSDL
CCEEECCCCCCCCCHHHHHHEEEEECCCCHHHCCCEEEEECCCEEEECCCCEEECCCHHH
ADRLLSLHQQLTAVVSTFAPDEAAVEQTFVNKDGAGTLKLGQARGIAMLVPAQAGLRVGE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEECCCCCCCCCC
YAPNKIKKTVVGVGHADKAQVLHMVRLQLPGAEIKSPDAADALAIAICHAHHGGAAGLGA
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
AIAKATA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA