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The map label for this gene is norM [H]

Identifier: 110679341

GI number: 110679341

Start: 1966561

End: 1968069

Strand: Direct

Name: norM [H]

Synonym: RD1_2060

Alternate gene names: 110679341

Gene position: 1966561-1968069 (Clockwise)

Preceding gene: 110679336

Following gene: 110679346

Centisome position: 47.58

GC content: 60.5

Gene sequence:

>1509_bases
GTGATCAGTATCGTATCCGCGTGGCCTGAAAATGTGTCACGGATACAGGATGGCTGTGTCGGTTGTGTCGCAAATTGTCC
TGATCCGGGCGGCTGGCCTCTTTCGTTCGGGGCAACGCAGGCGTATGGAAAACCAATGCAAGCCAGTGTCACATATCCCC
AGCACGCACGTGCCATCCTGAGCCTCGGCCTGCCGCTTGTCGGCGGGCATTTGGCGCAGATTGCCATCGGCATGACCGAC
ACGGTCATGTTGGGGTGGTATTCAGTCGAAGCGCTGGCGGCGGTGACGCTGGGGTCTACCTATTTCTTTGTGCTGTTCAT
CTTCGGGTCGGGTTTTGCCTGGGCTGTGATGCCCATGGTGGCGTCTTTCGCGGCAAAAGGGGATGAAATCGGGCTGCGCC
GCTGCACGCGCATGGCGCTCTGGCTCAGCCTTGGGTTCAGCTTTCTCGCCATGCCGTTGATGATCTGGTCCGAGCCGATT
TTGCATCTGATCGGGCAGGAGGACAGTGTTGCAAGAGATGCCAGCGCCTACCTTGAGGTGGCGGCCTATGGCATCATACC
CGCCTTGCTGGTGATGGTGATCAAATCCTATCTGGCGGCGTTGGAGCGCACGCAGGTGGTGCTTTGGGTGACGATCCTGG
CGGCGGTGGTCAATGCGATCGCCAATTATGCGCTGATCTTCGGCAACTGGGGTGCGCCGGAACTTGGCATCAGGGGGGCC
GCGATTGCATCGCTGATCACGCAAACGGTGTCGCTGATCGGGGTGGTGGTTTATGCGCGGCTCAGCTTGCCGGAGCATGC
CCTGTTCCAGCGCCTGTGGCGGGCCGACTGGCAGATGTTGCTGCAAGTTTTTCTGTTGGGCTGGCCCATCGGTCTTACCG
CGCTGGCAGAAGTGGGCCTGTTCGCAGGTTCCGCCGTAATGATGGGATGGCTGGGCACGGTGCCGCTGGCGGCCCATGGG
ATTGCGTTACAACTGGCGTCGATCACCTTCATGGTGCATCTGGGTCTGTCCAATGTCGCCACAATTCGCGCGGGCAACGC
TCATGCGCGTAATGACCCGGTTCAGATGGGGCGGGGCGGCAAGATGGTCACCTTCATGTCGCTTGGCTTTGCGGCCCTGA
CGATCATCATTTTCCTCGCCGTACCCGAAACGCTTTTGTCGCTGTTCATGCAAGAAGACGACCCGCAGCGCGCGGGGATC
ATGGCCATCGGCGTTGGGCTCCTGGCGATGGCCGCCCTGTTTCAACTGGTGGATGGGGCCCAGGTCATCGCACTGGGACT
CTTGCGCGGGGTGCAAGACACCAAGATTCCGATGATCTATGCCGCCGTCAGCTATTGGGGCGTGGGCATCCCGTGCTCTT
ATGTGTTTGGGTTCGTTCTTGGGTTGGACGGGATCGGCGTGTGGCTCGGCCTCGTTGTCGGGTTGTCGGTGGCGGCGCTC
CTGTTGTCACAGCGATTTTGGGGCGTTACTTTGCGCACGCTCAAGGCCACCGAGGGAGAGCCGCCTTAA

Upstream 100 bases:

>100_bases
TCTTGGGCTCCGGTCGGTGCCGCCGTAATTGACAGCACCCGTAAAGTGACAGACTCCCCCAGAAAATCAAGCGCTTAAGA
ACTGTTTTTTAGGTTGACCC

Downstream 100 bases:

>100_bases
AGCGTCATGCGAAAAACCCGGATCACGCAACGCTGCCTGACATCAAAGATTTCAACGCGTTACGTGGTTCTTGATGTTTC
GGTTAATTCGTCAGGCGCAG

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MISIVSAWPENVSRIQDGCVGCVANCPDPGGWPLSFGATQAYGKPMQASVTYPQHARAILSLGLPLVGGHLAQIAIGMTD
TVMLGWYSVEALAAVTLGSTYFFVLFIFGSGFAWAVMPMVASFAAKGDEIGLRRCTRMALWLSLGFSFLAMPLMIWSEPI
LHLIGQEDSVARDASAYLEVAAYGIIPALLVMVIKSYLAALERTQVVLWVTILAAVVNAIANYALIFGNWGAPELGIRGA
AIASLITQTVSLIGVVVYARLSLPEHALFQRLWRADWQMLLQVFLLGWPIGLTALAEVGLFAGSAVMMGWLGTVPLAAHG
IALQLASITFMVHLGLSNVATIRAGNAHARNDPVQMGRGGKMVTFMSLGFAALTIIIFLAVPETLLSLFMQEDDPQRAGI
MAIGVGLLAMAALFQLVDGAQVIALGLLRGVQDTKIPMIYAAVSYWGVGIPCSYVFGFVLGLDGIGVWLGLVVGLSVAAL
LLSQRFWGVTLRTLKATEGEPP

Sequences:

>Translated_502_residues
MISIVSAWPENVSRIQDGCVGCVANCPDPGGWPLSFGATQAYGKPMQASVTYPQHARAILSLGLPLVGGHLAQIAIGMTD
TVMLGWYSVEALAAVTLGSTYFFVLFIFGSGFAWAVMPMVASFAAKGDEIGLRRCTRMALWLSLGFSFLAMPLMIWSEPI
LHLIGQEDSVARDASAYLEVAAYGIIPALLVMVIKSYLAALERTQVVLWVTILAAVVNAIANYALIFGNWGAPELGIRGA
AIASLITQTVSLIGVVVYARLSLPEHALFQRLWRADWQMLLQVFLLGWPIGLTALAEVGLFAGSAVMMGWLGTVPLAAHG
IALQLASITFMVHLGLSNVATIRAGNAHARNDPVQMGRGGKMVTFMSLGFAALTIIIFLAVPETLLSLFMQEDDPQRAGI
MAIGVGLLAMAALFQLVDGAQVIALGLLRGVQDTKIPMIYAAVSYWGVGIPCSYVFGFVLGLDGIGVWLGLVVGLSVAAL
LLSQRFWGVTLRTLKATEGEPP
>Mature_502_residues
MISIVSAWPENVSRIQDGCVGCVANCPDPGGWPLSFGATQAYGKPMQASVTYPQHARAILSLGLPLVGGHLAQIAIGMTD
TVMLGWYSVEALAAVTLGSTYFFVLFIFGSGFAWAVMPMVASFAAKGDEIGLRRCTRMALWLSLGFSFLAMPLMIWSEPI
LHLIGQEDSVARDASAYLEVAAYGIIPALLVMVIKSYLAALERTQVVLWVTILAAVVNAIANYALIFGNWGAPELGIRGA
AIASLITQTVSLIGVVVYARLSLPEHALFQRLWRADWQMLLQVFLLGWPIGLTALAEVGLFAGSAVMMGWLGTVPLAAHG
IALQLASITFMVHLGLSNVATIRAGNAHARNDPVQMGRGGKMVTFMSLGFAALTIIIFLAVPETLLSLFMQEDDPQRAGI
MAIGVGLLAMAALFQLVDGAQVIALGLLRGVQDTKIPMIYAAVSYWGVGIPCSYVFGFVLGLDGIGVWLGLVVGLSVAAL
LLSQRFWGVTLRTLKATEGEPP

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI153792564, Length=323, Percent_Identity=26.9349845201238, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI38261960, Length=281, Percent_Identity=26.6903914590747, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI22907060, Length=330, Percent_Identity=25.7575757575758, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI48994890, Length=443, Percent_Identity=28.2167042889391, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI226510954, Length=468, Percent_Identity=24.7863247863248, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321821, Length=431, Percent_Identity=22.737819025522, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 53412; Mature: 53412

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIVSAWPENVSRIQDGCVGCVANCPDPGGWPLSFGATQAYGKPMQASVTYPQHARAIL
CCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHH
SLGLPLVGGHLAQIAIGMTDTVMLGWYSVEALAAVTLGSTYFFVLFIFGSGFAWAVMPMV
HHCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
ASFAAKGDEIGLRRCTRMALWLSLGFSFLAMPLMIWSEPILHLIGQEDSVARDASAYLEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AAYGIIPALLVMVIKSYLAALERTQVVLWVTILAAVVNAIANYALIFGNWGAPELGIRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
AIASLITQTVSLIGVVVYARLSLPEHALFQRLWRADWQMLLQVFLLGWPIGLTALAEVGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FAGSAVMMGWLGTVPLAAHGIALQLASITFMVHLGLSNVATIRAGNAHARNDPVQMGRGG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHCCCCC
KMVTFMSLGFAALTIIIFLAVPETLLSLFMQEDDPQRAGIMAIGVGLLAMAALFQLVDGA
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
QVIALGLLRGVQDTKIPMIYAAVSYWGVGIPCSYVFGFVLGLDGIGVWLGLVVGLSVAAL
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSQRFWGVTLRTLKATEGEPP
HHHHHHHHHHHHEEECCCCCCC
>Mature Secondary Structure
MISIVSAWPENVSRIQDGCVGCVANCPDPGGWPLSFGATQAYGKPMQASVTYPQHARAIL
CCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEECCCHHHHHHHH
SLGLPLVGGHLAQIAIGMTDTVMLGWYSVEALAAVTLGSTYFFVLFIFGSGFAWAVMPMV
HHCCHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHH
ASFAAKGDEIGLRRCTRMALWLSLGFSFLAMPLMIWSEPILHLIGQEDSVARDASAYLEV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AAYGIIPALLVMVIKSYLAALERTQVVLWVTILAAVVNAIANYALIFGNWGAPELGIRGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
AIASLITQTVSLIGVVVYARLSLPEHALFQRLWRADWQMLLQVFLLGWPIGLTALAEVGL
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FAGSAVMMGWLGTVPLAAHGIALQLASITFMVHLGLSNVATIRAGNAHARNDPVQMGRGG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHCCCCC
KMVTFMSLGFAALTIIIFLAVPETLLSLFMQEDDPQRAGIMAIGVGLLAMAALFQLVDGA
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
QVIALGLLRGVQDTKIPMIYAAVSYWGVGIPCSYVFGFVLGLDGIGVWLGLVVGLSVAAL
HHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSQRFWGVTLRTLKATEGEPP
HHHHHHHHHHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]