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The map label for this gene is glyS [H]

Identifier: 110679234

GI number: 110679234

Start: 1857118

End: 1859304

Strand: Direct

Name: glyS [H]

Synonym: RD1_1947

Alternate gene names: 110679234

Gene position: 1857118-1859304 (Clockwise)

Preceding gene: 110679233

Following gene: 110679235

Centisome position: 44.93

GC content: 59.26

Gene sequence:

>2187_bases
ATGCCTGATCTACTGATTGAACTCTTCTGTGAAGAAATCCCCGCCCGCATGCAACGCCGCGCGGCGCAGGATTTGCAAAA
ACTGGTGACTGATGGGCTGGTTGAGGCCGGGTTGACTTATGCCTCTGCGGCGGCGTTCTCGACACCGCGCCGTCTGACCC
TCACGCTTGAGGGAGTGCTTGCGGCAAGCCCCACCACCGTCGAGGAACGCAAAGGCCCCAAGGCCGATGCACCCGACAAG
GCCATCGAGGGTTTCCTGCGCGGTGCAGGTGTCGCGCGCGAGGCACTCGAGACGCGCGATACGCCCAAAGGGCCGGTGCT
CTTTGCCAAAATCACCAAGGAGGGACGGCCCGCCGCGCAGATCGTGGCGGAAGTTCTGGAAAAGACGATCCGTAACTTTC
CTTGGCCCAAATCCATGCGCTGGGGTGCGGGCACCCTGCGCTGGGTGCGCCCGTTACACTCGATCCTGTGCATTCTGTCC
GATGAGGCAGGTACGCAGGTCGTACCGCTGAGCGTCGATCACATCACGGCGGGGGACCACACACAGGGGCATCGTTTCAT
GGCGCCGGACCGCTTTACGGTCACCGGCTTTGATGACTATGCGGCCAAGCTGAAACGCGCCTTTGTCGTGCTCGACCCGG
CAGAGCGGGCAGATATGATCTGGCACGATGCCACCAATCAGGCCTTTGCCAATGGGCTTGAGGTGGTTGAGGATGCGGCC
CTGCTGGCCGAAGTTGCGGGGCTTGTGGAATGGCCCGTGGTGCTGATGGGGCGGATTGGTGATGATTTCCTCGATCTGCC
GCCCGAGGTGCTGCAAACATCGATGAAAGAGCATCAGAAATTCTTCTCCGTGCGCAATCCCAAGACGGGCCGGATCGAGC
GGTTCGTCACCGTGGCCAACCGCGATACGGCGGATAACGGCGCGACCATTCTGGCGGGGAATGAAAAGGTGCTGTCGGCG
CGTTTGGCGGATGCGAAATTCTTTTGGGACAATGATTTGCGCGTGGCCAAGGCGGGCATGGGCGACTGGCTGAAAGCCTT
GGAAAATGTGACCTTCCACAACAAGTTGGGCACGCAGGCGGAGCGGATCGCCCGCGTCGCGGCCCTTGCCCGGGAGCTGG
CCCCGATGGTGGGTGCGGACTCGGACGAGGCCGAGGAGGCCGCGCGGTTCGCCAAGGCGGACCTGTCGTCTGAAATGGTC
TATGAATTCCCTGAATTGCAAGGCTTGATGGGGCGCTACTACCTGAAAGCTGCCGGGAAGTCTGACGCCATCGCGGCGGC
GGCTGAGGAACACTACGCGCCCTTAGGGCCATCCGACGCCGTACCCACCGCCTCAGTCAGCGTCGCTGTGGCGCTGGCCG
ACAAACTTGACCAATTTACTGGAATGTGGGCTGCGAATGAGCTGCCAACTGGTTCAAAAGATCCTTATGCGCTGCGACGC
GCGGCTCTCAGCACCATTCGGCTAATTCTCGAAAATAGGCTCAAATTACCGCTCAAAAATCTGTTGGAAATATCATTTTC
CGACCGATACGAAATTGCCAAACTCGAAGTGAAGCGTAGTGTTGAAAGGGTTGCTCATAGGTATCCAACAGAAAAGGCAG
AGCTAAAAAAGATACTGGAGGAGGAGTTGTTGAAGATTTCCTCTACGGTAGACACTCGCAAAAATGGCTTATCCGAATTC
TTCCACGACCGCCTCAAAGTCTATCTCCGCGATCAAGGCATCCGCCACGACGTGATCGACGCGTGCATCGCCATGCCGGG
CAATGATGACCTGACATTGCTGGTCAAACGGGCCAAGGCGCTGTCCGATGTGGTCGGGACCGAGGATGGAGAAAACCTGA
TCCAGGGCTTCAAGCGGGCCAACAACATCCTGACGCAGGCGCAGGAGGCGGATGGGGTCGAATACTCCTACGGCGCGGAC
GCCAAATTCGCCGAAACCGACGAAGAACGCGCGCTGTTCAAAGCGCTGGACACCGCTGAGGCGATCATCACCCCGGCGAT
GAAATCCGAAGACTTTGCCACCGCCATGTCCGCCATGGCCACACTGCGCGCGCCGATTGATGCGTTTTTTGAGGCGGTGC
AGGTCAATTCTGACAACCCTACAGTCCGGCGCAACAGGCTCAACCTGCTCAATCGGATCACGCAGATCTGCCTGTCAGTT
GCGGATCTGACCCGGATCGAGGGCTGA

Upstream 100 bases:

>100_bases
ACGGGCGCGGCTACATCTGGCACGAGCTGAATGACTGCGGCGAAAAGGCTTATGACGGCACCATCATTGGTGAAGAATGC
CCCAGACGGGAAAATAACTG

Downstream 100 bases:

>100_bases
ACCGACCTGCGGCAAAATGGGAGCGCTTGCACTAGTGCTTGTCAGGCTAGGTTTACATTCTATGCTGCAGGCAAGTTCAA
AAAGGTGCCGCAGTGCAGAA

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MPDLLIELFCEEIPARMQRRAAQDLQKLVTDGLVEAGLTYASAAAFSTPRRLTLTLEGVLAASPTTVEERKGPKADAPDK
AIEGFLRGAGVAREALETRDTPKGPVLFAKITKEGRPAAQIVAEVLEKTIRNFPWPKSMRWGAGTLRWVRPLHSILCILS
DEAGTQVVPLSVDHITAGDHTQGHRFMAPDRFTVTGFDDYAAKLKRAFVVLDPAERADMIWHDATNQAFANGLEVVEDAA
LLAEVAGLVEWPVVLMGRIGDDFLDLPPEVLQTSMKEHQKFFSVRNPKTGRIERFVTVANRDTADNGATILAGNEKVLSA
RLADAKFFWDNDLRVAKAGMGDWLKALENVTFHNKLGTQAERIARVAALARELAPMVGADSDEAEEAARFAKADLSSEMV
YEFPELQGLMGRYYLKAAGKSDAIAAAAEEHYAPLGPSDAVPTASVSVAVALADKLDQFTGMWAANELPTGSKDPYALRR
AALSTIRLILENRLKLPLKNLLEISFSDRYEIAKLEVKRSVERVAHRYPTEKAELKKILEEELLKISSTVDTRKNGLSEF
FHDRLKVYLRDQGIRHDVIDACIAMPGNDDLTLLVKRAKALSDVVGTEDGENLIQGFKRANNILTQAQEADGVEYSYGAD
AKFAETDEERALFKALDTAEAIITPAMKSEDFATAMSAMATLRAPIDAFFEAVQVNSDNPTVRRNRLNLLNRITQICLSV
ADLTRIEG

Sequences:

>Translated_728_residues
MPDLLIELFCEEIPARMQRRAAQDLQKLVTDGLVEAGLTYASAAAFSTPRRLTLTLEGVLAASPTTVEERKGPKADAPDK
AIEGFLRGAGVAREALETRDTPKGPVLFAKITKEGRPAAQIVAEVLEKTIRNFPWPKSMRWGAGTLRWVRPLHSILCILS
DEAGTQVVPLSVDHITAGDHTQGHRFMAPDRFTVTGFDDYAAKLKRAFVVLDPAERADMIWHDATNQAFANGLEVVEDAA
LLAEVAGLVEWPVVLMGRIGDDFLDLPPEVLQTSMKEHQKFFSVRNPKTGRIERFVTVANRDTADNGATILAGNEKVLSA
RLADAKFFWDNDLRVAKAGMGDWLKALENVTFHNKLGTQAERIARVAALARELAPMVGADSDEAEEAARFAKADLSSEMV
YEFPELQGLMGRYYLKAAGKSDAIAAAAEEHYAPLGPSDAVPTASVSVAVALADKLDQFTGMWAANELPTGSKDPYALRR
AALSTIRLILENRLKLPLKNLLEISFSDRYEIAKLEVKRSVERVAHRYPTEKAELKKILEEELLKISSTVDTRKNGLSEF
FHDRLKVYLRDQGIRHDVIDACIAMPGNDDLTLLVKRAKALSDVVGTEDGENLIQGFKRANNILTQAQEADGVEYSYGAD
AKFAETDEERALFKALDTAEAIITPAMKSEDFATAMSAMATLRAPIDAFFEAVQVNSDNPTVRRNRLNLLNRITQICLSV
ADLTRIEG
>Mature_727_residues
PDLLIELFCEEIPARMQRRAAQDLQKLVTDGLVEAGLTYASAAAFSTPRRLTLTLEGVLAASPTTVEERKGPKADAPDKA
IEGFLRGAGVAREALETRDTPKGPVLFAKITKEGRPAAQIVAEVLEKTIRNFPWPKSMRWGAGTLRWVRPLHSILCILSD
EAGTQVVPLSVDHITAGDHTQGHRFMAPDRFTVTGFDDYAAKLKRAFVVLDPAERADMIWHDATNQAFANGLEVVEDAAL
LAEVAGLVEWPVVLMGRIGDDFLDLPPEVLQTSMKEHQKFFSVRNPKTGRIERFVTVANRDTADNGATILAGNEKVLSAR
LADAKFFWDNDLRVAKAGMGDWLKALENVTFHNKLGTQAERIARVAALARELAPMVGADSDEAEEAARFAKADLSSEMVY
EFPELQGLMGRYYLKAAGKSDAIAAAAEEHYAPLGPSDAVPTASVSVAVALADKLDQFTGMWAANELPTGSKDPYALRRA
ALSTIRLILENRLKLPLKNLLEISFSDRYEIAKLEVKRSVERVAHRYPTEKAELKKILEEELLKISSTVDTRKNGLSEFF
HDRLKVYLRDQGIRHDVIDACIAMPGNDDLTLLVKRAKALSDVVGTEDGENLIQGFKRANNILTQAQEADGVEYSYGADA
KFAETDEERALFKALDTAEAIITPAMKSEDFATAMSAMATLRAPIDAFFEAVQVNSDNPTVRRNRLNLLNRITQICLSVA
DLTRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=736, Percent_Identity=32.2010869565217, Blast_Score=341, Evalue=1e-94,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80056; Mature: 79925

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLIELFCEEIPARMQRRAAQDLQKLVTDGLVEAGLTYASAAAFSTPRRLTLTLEGVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEEHH
AASPTTVEERKGPKADAPDKAIEGFLRGAGVAREALETRDTPKGPVLFAKITKEGRPAAQ
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHH
IVAEVLEKTIRNFPWPKSMRWGAGTLRWVRPLHSILCILSDEAGTQVVPLSVDHITAGDH
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCC
TQGHRFMAPDRFTVTGFDDYAAKLKRAFVVLDPAERADMIWHDATNQAFANGLEVVEDAA
CCCCEEECCCCEEEECCHHHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLAEVAGLVEWPVVLMGRIGDDFLDLPPEVLQTSMKEHQKFFSVRNPKTGRIERFVTVAN
HHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECC
RDTADNGATILAGNEKVLSARLADAKFFWDNDLRVAKAGMGDWLKALENVTFHNKLGTQA
CCCCCCCCEEEECCHHHHHHHHHCCEEEECCCCCEECCCHHHHHHHHHCCCHHHHCCHHH
ERIARVAALARELAPMVGADSDEAEEAARFAKADLSSEMVYEFPELQGLMGRYYLKAAGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCC
SDAIAAAAEEHYAPLGPSDAVPTASVSVAVALADKLDQFTGMWAANELPTGSKDPYALRR
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AALSTIRLILENRLKLPLKNLLEISFSDRYEIAKLEVKRSVERVAHRYPTEKAELKKILE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EELLKISSTVDTRKNGLSEFFHDRLKVYLRDQGIRHDVIDACIAMPGNDDLTLLVKRAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
LSDVVGTEDGENLIQGFKRANNILTQAQEADGVEYSYGADAKFAETDEERALFKALDTAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
AIITPAMKSEDFATAMSAMATLRAPIDAFFEAVQVNSDNPTVRRNRLNLLNRITQICLSV
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADLTRIEG
HHHHHCCC
>Mature Secondary Structure 
PDLLIELFCEEIPARMQRRAAQDLQKLVTDGLVEAGLTYASAAAFSTPRRLTLTLEGVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEEEHH
AASPTTVEERKGPKADAPDKAIEGFLRGAGVAREALETRDTPKGPVLFAKITKEGRPAAQ
CCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHH
IVAEVLEKTIRNFPWPKSMRWGAGTLRWVRPLHSILCILSDEAGTQVVPLSVDHITAGDH
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECEEECCCC
TQGHRFMAPDRFTVTGFDDYAAKLKRAFVVLDPAERADMIWHDATNQAFANGLEVVEDAA
CCCCEEECCCCEEEECCHHHHHHHHHHEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHH
LLAEVAGLVEWPVVLMGRIGDDFLDLPPEVLQTSMKEHQKFFSVRNPKTGRIERFVTVAN
HHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEECC
RDTADNGATILAGNEKVLSARLADAKFFWDNDLRVAKAGMGDWLKALENVTFHNKLGTQA
CCCCCCCCEEEECCHHHHHHHHHCCEEEECCCCCEECCCHHHHHHHHHCCCHHHHCCHHH
ERIARVAALARELAPMVGADSDEAEEAARFAKADLSSEMVYEFPELQGLMGRYYLKAAGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHCCC
SDAIAAAAEEHYAPLGPSDAVPTASVSVAVALADKLDQFTGMWAANELPTGSKDPYALRR
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
AALSTIRLILENRLKLPLKNLLEISFSDRYEIAKLEVKRSVERVAHRYPTEKAELKKILE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EELLKISSTVDTRKNGLSEFFHDRLKVYLRDQGIRHDVIDACIAMPGNDDLTLLVKRAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
LSDVVGTEDGENLIQGFKRANNILTQAQEADGVEYSYGADAKFAETDEERALFKALDTAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
AIITPAMKSEDFATAMSAMATLRAPIDAFFEAVQVNSDNPTVRRNRLNLLNRITQICLSV
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADLTRIEG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA