Click here to switch to the map view.

The map label for this gene is sdaA [H]

Identifier: 110679206

GI number: 110679206

Start: 1828342

End: 1829718

Strand: Reverse

Name: sdaA [H]

Synonym: RD1_1917

Alternate gene names: 110679206

Gene position: 1829718-1828342 (Counterclockwise)

Preceding gene: 110679213

Following gene: 110679205

Centisome position: 44.27

GC content: 60.78

Gene sequence:

>1377_bases
ATGTTTTTATCTGTCTTTGATATGTTCAAGGTCGGTATCGGCCCCTCATCCTCGCACACCATGGGCCCCATGGTGGCCGC
CGCACGGTTCCTTGACATGATGCGCGCCTCGCCATTCGAATTTCACGGGGTGCGCGTGTCGCTGCATGGCTCGCTTGCCT
TTACAGGTGTGGGTCATGCCACGGACCGGGCCACCATTCTAGGGCTCGCGGGGTTTGTCCCCGATACCTATGACGCCGAC
AAGGCCGAAGCCGCGCTCGCAAAGATCAAAGAGACAAAGACGCTGAACGTCGATGGCCTGCCCCCGCTGCGGTTTGACCC
GGCAAGCGATCTGATGTTCGACTATGAGACGCCGCTCACTGGGCATGCAAACGGTATGATGATCCGCGCCACGGATGCCC
AGGGCGATGTCACTCTGCGCGAGGTTTTCTATTCCATCGGCGGTGGATTTGTGATGACCGAAGCCGAACTCGCAGCGGGC
AAGGACACCGATGAAAGCGGTCCGCCCATCCCCTACCCGTTCAAATCCGCCGCCCAGATGCTTGAGATGTCCACCGCCTC
CGGCAAGAACATTGCCGAAATGAAGCGGGCGAATGAAATTGCCCGTGGCTGTCCGACCAGCCTCGCAGAAGGCACCAAAC
GGCTGTGGCAGGTGATGGATGACTGCATCACGCGTGGGCTGACGACGGATGGAATTTTGCCCGGCGGCCTGCAGGTCAAA
CGCCGTGCCAAGGGCATCTATGATGCGTTGATGGCTGAACGGGGCATGAACCTGAACGCGCCCCATACGATCAATGACTG
GATGAGCGTCTACGCCATGGCGGTCAACGAGGAAAACGCCGCCGGTGGGCAGGTGGTCACCGCCCCCACAAATGGTGCTG
CGGGCGTTTTGCCAGCCACCTTGCGCTATTATCTCGATCATGTCCCGGGCGCGTCAGAAGCGCATATCGAGGATTTTCTA
TTGACGGCGGCCGCCATTGGCGGATTGGTCAAGTTCAATGCGTCGATTTCAGGCGCTGAGGCGGGCTGTCAGGCCGAGGT
CGGCTCTGCCGCGGCGATGGCCGCTGCGGGGCTGTGCGCCGTGATGGGCGGCACCCCCGCTCAGGTGGAAAACGCCGCGG
AAATCGCCCTCGAACACCACCTTGGCATGACCTGTGATCCGGTCAAGGGTCTGGTGCAGGTGCCCTGCATCGAACGCAAT
GGCCTTGGCGCAATCAAGGCCGTATCAGCGGCTTCGCTGGCCCTGCGCGGCGATGGTACGCATCTGGTTCCGCTGGACGC
ATGCATCGAAACCATGCGCCAGACCGGCCTTGATATGTCCGAGAAGTACAAGGAAACCTCGCTTGGCGGATTGGCGGTAA
ACGTGCCGAACTGCTGA

Upstream 100 bases:

>100_bases
GCGCCCGCCCGTAAAGATCGCACAACCGGCCGAAGGGTTGTGCAGCAGCCCAGTGACTCCCCCACGCCATCCAGACCCGC
CAGAAGGAAGTGCCACCTAA

Downstream 100 bases:

>100_bases
GCAGGAAAAACAACCTGTGCTTGCAATGCCATTGGGATCGCCTTAGGCTTGATGCATGACAATTGACCGACCTTTCCTTG
GCATCATGCTGATGCTGGGA

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFEFHGVRVSLHGSLAFTGVGHATDRATILGLAGFVPDTYDAD
KAEAALAKIKETKTLNVDGLPPLRFDPASDLMFDYETPLTGHANGMMIRATDAQGDVTLREVFYSIGGGFVMTEAELAAG
KDTDESGPPIPYPFKSAAQMLEMSTASGKNIAEMKRANEIARGCPTSLAEGTKRLWQVMDDCITRGLTTDGILPGGLQVK
RRAKGIYDALMAERGMNLNAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPATLRYYLDHVPGASEAHIEDFL
LTAAAIGGLVKFNASISGAEAGCQAEVGSAAAMAAAGLCAVMGGTPAQVENAAEIALEHHLGMTCDPVKGLVQVPCIERN
GLGAIKAVSAASLALRGDGTHLVPLDACIETMRQTGLDMSEKYKETSLGGLAVNVPNC

Sequences:

>Translated_458_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFEFHGVRVSLHGSLAFTGVGHATDRATILGLAGFVPDTYDAD
KAEAALAKIKETKTLNVDGLPPLRFDPASDLMFDYETPLTGHANGMMIRATDAQGDVTLREVFYSIGGGFVMTEAELAAG
KDTDESGPPIPYPFKSAAQMLEMSTASGKNIAEMKRANEIARGCPTSLAEGTKRLWQVMDDCITRGLTTDGILPGGLQVK
RRAKGIYDALMAERGMNLNAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPATLRYYLDHVPGASEAHIEDFL
LTAAAIGGLVKFNASISGAEAGCQAEVGSAAAMAAAGLCAVMGGTPAQVENAAEIALEHHLGMTCDPVKGLVQVPCIERN
GLGAIKAVSAASLALRGDGTHLVPLDACIETMRQTGLDMSEKYKETSLGGLAVNVPNC
>Mature_458_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFEFHGVRVSLHGSLAFTGVGHATDRATILGLAGFVPDTYDAD
KAEAALAKIKETKTLNVDGLPPLRFDPASDLMFDYETPLTGHANGMMIRATDAQGDVTLREVFYSIGGGFVMTEAELAAG
KDTDESGPPIPYPFKSAAQMLEMSTASGKNIAEMKRANEIARGCPTSLAEGTKRLWQVMDDCITRGLTTDGILPGGLQVK
RRAKGIYDALMAERGMNLNAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPATLRYYLDHVPGASEAHIEDFL
LTAAAIGGLVKFNASISGAEAGCQAEVGSAAAMAAAGLCAVMGGTPAQVENAAEIALEHHLGMTCDPVKGLVQVPCIERN
GLGAIKAVSAASLALRGDGTHLVPLDACIETMRQTGLDMSEKYKETSLGGLAVNVPNC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI48994925, Length=457, Percent_Identity=47.7024070021882, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1789161, Length=460, Percent_Identity=46.0869565217391, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788116, Length=457, Percent_Identity=46.1706783369803, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 47914; Mature: 47914

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFEFHGVRVSLHGSLAFTGVGHA
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEECCCC
TDRATILGLAGFVPDTYDADKAEAALAKIKETKTLNVDGLPPLRFDPASDLMFDYETPLT
CCCHHEEEHHHCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEEECCCCCC
GHANGMMIRATDAQGDVTLREVFYSIGGGFVMTEAELAAGKDTDESGPPIPYPFKSAAQM
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
LEMSTASGKNIAEMKRANEIARGCPTSLAEGTKRLWQVMDDCITRGLTTDGILPGGLQVK
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RRAKGIYDALMAERGMNLNAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCHHHHH
LRYYLDHVPGASEAHIEDFLLTAAAIGGLVKFNASISGAEAGCQAEVGSAAAMAAAGLCA
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCHHHHHHHHHHHH
VMGGTPAQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNGLGAIKAVSAASLALRGDGT
HCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHEEEECCCC
HLVPLDACIETMRQTGLDMSEKYKETSLGGLAVNVPNC
EEEEHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFEFHGVRVSLHGSLAFTGVGHA
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCEEEEECCCC
TDRATILGLAGFVPDTYDADKAEAALAKIKETKTLNVDGLPPLRFDPASDLMFDYETPLT
CCCHHEEEHHHCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCEEECCCCCC
GHANGMMIRATDAQGDVTLREVFYSIGGGFVMTEAELAAGKDTDESGPPIPYPFKSAAQM
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCCCHHHHHHH
LEMSTASGKNIAEMKRANEIARGCPTSLAEGTKRLWQVMDDCITRGLTTDGILPGGLQVK
HHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RRAKGIYDALMAERGMNLNAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAT
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCCCCCCCHHHHH
LRYYLDHVPGASEAHIEDFLLTAAAIGGLVKFNASISGAEAGCQAEVGSAAAMAAAGLCA
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCHHHHHHHHHHHH
VMGGTPAQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNGLGAIKAVSAASLALRGDGT
HCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHEEEECCCC
HLVPLDACIETMRQTGLDMSEKYKETSLGGLAVNVPNC
EEEEHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]