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The map label for this gene is folA [H]

Identifier: 110679165

GI number: 110679165

Start: 1793869

End: 1794348

Strand: Direct

Name: folA [H]

Synonym: RD1_1875

Alternate gene names: 110679165

Gene position: 1793869-1794348 (Clockwise)

Preceding gene: 110679164

Following gene: 110679167

Centisome position: 43.4

GC content: 55.62

Gene sequence:

>480_bases
ATGATCACGCTTATTGTGGCACGCGCTGAAAACGGCGCGATCGGTCGAGACGGGACAATTCCATGGGACATTCCCGAGGA
CCTCAAGTTTTTTCAGCGCGAAACCCTTGGGGGGGCGTTGATCATGGGACGCAACACCTGGGAGAGCCTGCCGGTCAAAC
CTTTGCCCCGACGTTTCAATATCGTGGTATCGTCGAATTCGGATATCGCCGAAGTGGTCGCGCCAAGTGTCGAGACGGCA
ATTGAGATTGCAAAAGCACAAGACCACCAGCGCATCTATGGTATCGGCGGCGCGTCTATCTACGCAGAAATGCTGCCGCT
TGCCCATCGACTCCTCATCTCGGAAGTTCAGCTTCAGGTGCCAGACGCGGATACTTTTTTCCCGCACGTCGACTTGGCCG
AATGGCACCAGATCGACAGGCGATCCCTGCGCGAGAGCGATCCTGCGTGTTTTGTGGTTGAACATTTGAGACGAGACTGA

Upstream 100 bases:

>100_bases
CAGGCAGGTGAGTTCCATTTTCGACTTCCGCTATGAGGATTTTGAAGTGATCGGCTATGATCCAGACCCGGCTATACGGG
CACCTGTGGCGGTCTGAGGT

Downstream 100 bases:

>100_bases
CGCAGTGCAGGATGGTTGTCATCTGCTCTGGTTGACAAGCACGATGCCGACATATGCCGTCGCACTGCTCGGGCCAGATT
TCGTGGCCCAAGCCTCGTAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MITLIVARAENGAIGRDGTIPWDIPEDLKFFQRETLGGALIMGRNTWESLPVKPLPRRFNIVVSSNSDIAEVVAPSVETA
IEIAKAQDHQRIYGIGGASIYAEMLPLAHRLLISEVQLQVPDADTFFPHVDLAEWHQIDRRSLRESDPACFVVEHLRRD

Sequences:

>Translated_159_residues
MITLIVARAENGAIGRDGTIPWDIPEDLKFFQRETLGGALIMGRNTWESLPVKPLPRRFNIVVSSNSDIAEVVAPSVETA
IEIAKAQDHQRIYGIGGASIYAEMLPLAHRLLISEVQLQVPDADTFFPHVDLAEWHQIDRRSLRESDPACFVVEHLRRD
>Mature_159_residues
MITLIVARAENGAIGRDGTIPWDIPEDLKFFQRETLGGALIMGRNTWESLPVKPLPRRFNIVVSSNSDIAEVVAPSVETA
IEIAKAQDHQRIYGIGGASIYAEMLPLAHRLLISEVQLQVPDADTFFPHVDLAEWHQIDRRSLRESDPACFVVEHLRRD

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=154, Percent_Identity=29.8701298701299, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI28827793, Length=154, Percent_Identity=31.8181818181818, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1786233, Length=138, Percent_Identity=37.6811594202899, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17505899, Length=172, Percent_Identity=29.6511627906977, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=161, Percent_Identity=31.6770186335404, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI116008026, Length=146, Percent_Identity=38.3561643835616, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647458, Length=146, Percent_Identity=38.3561643835616, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17837; Mature: 17837

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLIVARAENGAIGRDGTIPWDIPEDLKFFQRETLGGALIMGRNTWESLPVKPLPRRFN
CEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEE
IVVSSNSDIAEVVAPSVETAIEIAKAQDHQRIYGIGGASIYAEMLPLAHRLLISEVQLQV
EEEECCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEC
PDADTFFPHVDLAEWHQIDRRSLRESDPACFVVEHLRRD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCC
>Mature Secondary Structure
MITLIVARAENGAIGRDGTIPWDIPEDLKFFQRETLGGALIMGRNTWESLPVKPLPRRFN
CEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEE
IVVSSNSDIAEVVAPSVETAIEIAKAQDHQRIYGIGGASIYAEMLPLAHRLLISEVQLQV
EEEECCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHEEEC
PDADTFFPHVDLAEWHQIDRRSLRESDPACFVVEHLRRD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]