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The map label for this gene is thyA

Identifier: 110679164

GI number: 110679164

Start: 1793032

End: 1793865

Strand: Direct

Name: thyA

Synonym: RD1_1874

Alternate gene names: 110679164

Gene position: 1793032-1793865 (Clockwise)

Preceding gene: 110679163

Following gene: 110679165

Centisome position: 43.38

GC content: 56.95

Gene sequence:

>834_bases
TTGCAGCAGTATCACGACGCACTACGCACCATTCTGGAGTGCGGAGAAGAGTCAACGGACCGGACGGGCACCGGCACGAT
CTCCTACTTCGGTATGCAAATGCGCTACCCGCTCTCGGACGGTTTTCCGCTGGTGACGACGAAAAAGCTGCACCTGCGCT
CGATCTTTCATGAGTTGCTCTGGTTTCTCTCAGGGGATACGAACATCCGCTACCTGCAGGACAACGGAGTCTCGATCTGG
GATGAATGGGCCGATGAAAATGGCGACCTCGGACCGGTCTATGGCCACCAGTGGCGTGCGTTTTCTGCGCTTTCGCCGAC
GGAAGAGGTCAAAGATGGCGAGCCCTTGTACCTAGGCCGCGGCGTTGATCAGGTCACAAACCTGATTGACATGATCAAAT
CCAGTCCCGACAGCCGTCGTATGATCGTGTCCGCGTGGAACCCGGCGGATGTGCCGCGTATGGCGCTGCCGCCATGCCAC
GCGTTGTGGCAGGTGCGCGTGCTGAACGGTCGTATGCACCTGCAGCTTTATCAACGCTCCGCCGATATGTTCCTTGGCGT
GCCGTTCAACATCGCGTCCTATTCGTTGCTGCTGGTGATGCTGGCCCATGTCACGGGCTATGAACCGGGTGATTTCGTGC
ACACGCTGGGGGATGCGCATATTTACAGCAACCACATGGATCAGGTGAATTTGCAACTGACGCGGACACCCAAACCGCTG
CCGACACTACGAATAAACAGGCAGGTGAGTTCCATTTTCGACTTCCGCTATGAGGATTTTGAAGTGATCGGCTATGATCC
AGACCCGGCTATACGGGCACCTGTGGCGGTCTGA

Upstream 100 bases:

>100_bases
CGCGTAAATCAAAGGTCTGGGCAGAGCGCGATCTGCCTCTGGTCGAGCATGCCGCAGCCTTTCCGGCGCGGTGCATCCTG
AACGAATGATGGGGATTGAA

Downstream 100 bases:

>100_bases
GGTATGATCACGCTTATTGTGGCACGCGCTGAAAACGGCGCGATCGGTCGAGACGGGACAATTCCATGGGACATTCCCGA
GGACCTCAAGTTTTTTCAGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQQYHDALRTILECGEESTDRTGTGTISYFGMQMRYPLSDGFPLVTTKKLHLRSIFHELLWFLSGDTNIRYLQDNGVSIW
DEWADENGDLGPVYGHQWRAFSALSPTEEVKDGEPLYLGRGVDQVTNLIDMIKSSPDSRRMIVSAWNPADVPRMALPPCH
ALWQVRVLNGRMHLQLYQRSADMFLGVPFNIASYSLLLVMLAHVTGYEPGDFVHTLGDAHIYSNHMDQVNLQLTRTPKPL
PTLRINRQVSSIFDFRYEDFEVIGYDPDPAIRAPVAV

Sequences:

>Translated_277_residues
MQQYHDALRTILECGEESTDRTGTGTISYFGMQMRYPLSDGFPLVTTKKLHLRSIFHELLWFLSGDTNIRYLQDNGVSIW
DEWADENGDLGPVYGHQWRAFSALSPTEEVKDGEPLYLGRGVDQVTNLIDMIKSSPDSRRMIVSAWNPADVPRMALPPCH
ALWQVRVLNGRMHLQLYQRSADMFLGVPFNIASYSLLLVMLAHVTGYEPGDFVHTLGDAHIYSNHMDQVNLQLTRTPKPL
PTLRINRQVSSIFDFRYEDFEVIGYDPDPAIRAPVAV
>Mature_277_residues
MQQYHDALRTILECGEESTDRTGTGTISYFGMQMRYPLSDGFPLVTTKKLHLRSIFHELLWFLSGDTNIRYLQDNGVSIW
DEWADENGDLGPVYGHQWRAFSALSPTEEVKDGEPLYLGRGVDQVTNLIDMIKSSPDSRRMIVSAWNPADVPRMALPPCH
ALWQVRVLNGRMHLQLYQRSADMFLGVPFNIASYSLLLVMLAHVTGYEPGDFVHTLGDAHIYSNHMDQVNLQLTRTPKPL
PTLRINRQVSSIFDFRYEDFEVIGYDPDPAIRAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=287, Percent_Identity=54.3554006968641, Blast_Score=320, Evalue=8e-88,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=59.927797833935, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=48.2638888888889, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324648, Length=232, Percent_Identity=46.551724137931, Blast_Score=207, Evalue=1e-54,
Organism=Drosophila melanogaster, GI17137556, Length=288, Percent_Identity=51.7361111111111, Blast_Score=298, Evalue=3e-81,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_ROSDO (Q168V8)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682171.1
- ProteinModelPortal:   Q168V8
- SMR:   Q168V8
- STRING:   Q168V8
- GeneID:   4196855
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1874
- NMPDR:   fig|375451.6.peg.1751
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q168V8
- ProtClustDB:   PRK01827
- BioCyc:   RDEN375451:RD1_1874-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31544; Mature: 31544

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYHDALRTILECGEESTDRTGTGTISYFGMQMRYPLSDGFPLVTTKKLHLRSIFHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHH
WFLSGDTNIRYLQDNGVSIWDEWADENGDLGPVYGHQWRAFSALSPTEEVKDGEPLYLGR
HHHCCCCEEEEEECCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCEEEEC
GVDQVTNLIDMIKSSPDSRRMIVSAWNPADVPRMALPPCHALWQVRVLNGRMHLQLYQRS
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHEEEEEECCEEEEEEEECC
ADMFLGVPFNIASYSLLLVMLAHVTGYEPGDFVHTLGDAHIYSNHMDQVNLQLTRTPKPL
CCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCEEEEEEECCCCCC
PTLRINRQVSSIFDFRYEDFEVIGYDPDPAIRAPVAV
CCEEECHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQQYHDALRTILECGEESTDRTGTGTISYFGMQMRYPLSDGFPLVTTKKLHLRSIFHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHH
WFLSGDTNIRYLQDNGVSIWDEWADENGDLGPVYGHQWRAFSALSPTEEVKDGEPLYLGR
HHHCCCCEEEEEECCCCEEEHHHCCCCCCCCCCCCCCCEEECCCCCHHHHCCCCCEEEEC
GVDQVTNLIDMIKSSPDSRRMIVSAWNPADVPRMALPPCHALWQVRVLNGRMHLQLYQRS
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHEEEEEECCEEEEEEEECC
ADMFLGVPFNIASYSLLLVMLAHVTGYEPGDFVHTLGDAHIYSNHMDQVNLQLTRTPKPL
CCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCEEEEEEECCCCCC
PTLRINRQVSSIFDFRYEDFEVIGYDPDPAIRAPVAV
CCEEECHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA