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The map label for this gene is metB [H]

Identifier: 110679129

GI number: 110679129

Start: 1759240

End: 1760538

Strand: Reverse

Name: metB [H]

Synonym: RD1_1834

Alternate gene names: 110679129

Gene position: 1760538-1759240 (Counterclockwise)

Preceding gene: 110679131

Following gene: 110679126

Centisome position: 42.6

GC content: 60.74

Gene sequence:

>1299_bases
ATGACAGACCCAGAAGGTCCCAGCTACGGCTTTGACACGTTGCAGATTCACGCCGGCGCGCGTCCGGACCCTGCAACAGG
TGCACGGCAGACGCCCATTTACCAGACAACCGCCTATGTCTTTCGCGACACCGACCACGCAGCGGCCCTGTTCAACCTGC
AGGAAGTCGGATTCATCTATTCCCGGCTGACCAACCCGACGGTTGCGGTGCTGCAGGAGCGGATTGCAACCCTCGAAGGG
GGCGTGGGTGCAGCCTGCTGTTCCTCTGGCCATGCGGCCCAGATCATGGCGCTGTTTCCCCTGATGGGGCCAGGCAAGAA
CGTGGTGGCGTCAACCCGTCTTTACGGCGGCACGGTCACGCAGTTCAGCCACACGATCAAGCGGTTTGGCTGGGAATGCA
CCTTTGTCGATTTCGACGATCTGGACGCCGTCAAGGCCGCGATCAACGACGACACCCGCGCGGTGTTCTGCGAGGCGATT
GCCAATCCGGGCGGATATATCACCGATCTCGATGCGATTTCCAAGATATCTGACGCGGCGGGCATTCCGCTGATCGTGGA
TAACACATCCGCCACGCCATATCTGTGTCGCCCGATCGAACATGGTGCGACGCTGGTTGTGCACTCCACCACGAAATACC
TGACAGGCAACGGCACGGTGACGGGCGGCTGCATCGTGGATTCGGGCAAATTCGACTGGTCTGCCAATGACAAATTCCCG
TCGCTGAGCCAGCCGGAACCGGCCTATCACGGGCTGAAATTCCACGAAACCTTTGGCCCGCTGGCGTTTACCTTCCACTC
GATTGCCATCGGCCTGCGCGACCTCGGGATGACCATGAACCCGCAAGCCGCGCATTACACATTGATGGGGACAGAAACGC
TGTCGCTGCGGATGGACCGTCACGTGGAGAACGCGGAGACGATCACCAAGTGGCTGGAAAAAGACCCGCGGGTCGACTAC
GTGACCTACGCGGGCCTGCCGTCATCGCCCTACCATGACCGGGTGAAAAGGATTTGCCCAAAGGGTGCGGGCGGCCTGTT
CACCTTTGCGATCAAGGGCGGCTATGAAGCCTGCATCAAGTTCGTGAACGCGCTTGAAGTCTTCAGCCATGTGGCAAATC
TGGGTGATACACGATCCATCGTGACGCATTCGGCGTCCACCACGCACCGCCAGTTGACCCAAGAGCAGCAGGAGGCGGCA
GGGGCCGGGCCGGGCGTTGTGCGTATATCCATCGGGACCGAGGACGCCGACGACCTGATCGCCGATCTGGATCAGGCGCT
TGCCAAGGCCACCAGCTGA

Upstream 100 bases:

>100_bases
GCAGAGGGTTGCGGATGTGGCGGCCTGGGCGCGCGACCCTTGTACCCTTGTGCGGCTTGCCATAACCATGTGGCAAATCA
AGTCCTCAGGAGAACCCACG

Downstream 100 bases:

>100_bases
AAAGCATGAATTTGCCGGGAAAGGGCGGCCGCGCGCGGCCCTTTCTTTTTATCCTCAACCGTTTTCGTCCAGTATCCGGA
ACACCATGGACACCTGCGAC

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTDPEGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDTDHAAALFNLQEVGFIYSRLTNPTVAVLQERIATLEG
GVGAACCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSHTIKRFGWECTFVDFDDLDAVKAAINDDTRAVFCEAI
ANPGGYITDLDAISKISDAAGIPLIVDNTSATPYLCRPIEHGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSANDKFP
SLSQPEPAYHGLKFHETFGPLAFTFHSIAIGLRDLGMTMNPQAAHYTLMGTETLSLRMDRHVENAETITKWLEKDPRVDY
VTYAGLPSSPYHDRVKRICPKGAGGLFTFAIKGGYEACIKFVNALEVFSHVANLGDTRSIVTHSASTTHRQLTQEQQEAA
GAGPGVVRISIGTEDADDLIADLDQALAKATS

Sequences:

>Translated_432_residues
MTDPEGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDTDHAAALFNLQEVGFIYSRLTNPTVAVLQERIATLEG
GVGAACCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSHTIKRFGWECTFVDFDDLDAVKAAINDDTRAVFCEAI
ANPGGYITDLDAISKISDAAGIPLIVDNTSATPYLCRPIEHGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSANDKFP
SLSQPEPAYHGLKFHETFGPLAFTFHSIAIGLRDLGMTMNPQAAHYTLMGTETLSLRMDRHVENAETITKWLEKDPRVDY
VTYAGLPSSPYHDRVKRICPKGAGGLFTFAIKGGYEACIKFVNALEVFSHVANLGDTRSIVTHSASTTHRQLTQEQQEAA
GAGPGVVRISIGTEDADDLIADLDQALAKATS
>Mature_431_residues
TDPEGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDTDHAAALFNLQEVGFIYSRLTNPTVAVLQERIATLEGG
VGAACCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVTQFSHTIKRFGWECTFVDFDDLDAVKAAINDDTRAVFCEAIA
NPGGYITDLDAISKISDAAGIPLIVDNTSATPYLCRPIEHGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSANDKFPS
LSQPEPAYHGLKFHETFGPLAFTFHSIAIGLRDLGMTMNPQAAHYTLMGTETLSLRMDRHVENAETITKWLEKDPRVDYV
TYAGLPSSPYHDRVKRICPKGAGGLFTFAIKGGYEACIKFVNALEVFSHVANLGDTRSIVTHSASTTHRQLTQEQQEAAG
AGPGVVRISIGTEDADDLIADLDQALAKATS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=31.5165876777251, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=29.6208530805687, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=28.0285035629454, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790375, Length=425, Percent_Identity=29.1764705882353, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=25.8139534883721, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71980708, Length=416, Percent_Identity=30.0480769230769, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=32.387706855792, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17560056, Length=428, Percent_Identity=31.5420560747664, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=43.6781609195402, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=31.8604651162791, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=366, Percent_Identity=23.4972677595628, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321254, Length=450, Percent_Identity=24.6666666666667, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=32.4705882352941, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46373; Mature: 46241

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPEGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDTDHAAALFNLQEVGFIY
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
SRLTNPTVAVLQERIATLEGGVGAACCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVT
HHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHH
QFSHTIKRFGWECTFVDFDDLDAVKAAINDDTRAVFCEAIANPGGYITDLDAISKISDAA
HHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEHHHHCCCCCCEECHHHHHHHHHCC
GIPLIVDNTSATPYLCRPIEHGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSANDKFP
CCCEEEECCCCCCEEEEECCCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCC
SLSQPEPAYHGLKFHETFGPLAFTFHSIAIGLRDLGMTMNPQAAHYTLMGTETLSLRMDR
CCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEEHH
HVENAETITKWLEKDPRVDYVTYAGLPSSPYHDRVKRICPKGAGGLFTFAIKGGYEACIK
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
FVNALEVFSHVANLGDTRSIVTHSASTTHRQLTQEQQEAAGAGPGVVRISIGTEDADDLI
HHHHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
ADLDQALAKATS
HHHHHHHHHCCC
>Mature Secondary Structure 
TDPEGPSYGFDTLQIHAGARPDPATGARQTPIYQTTAYVFRDTDHAAALFNLQEVGFIY
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
SRLTNPTVAVLQERIATLEGGVGAACCSSGHAAQIMALFPLMGPGKNVVASTRLYGGTVT
HHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHH
QFSHTIKRFGWECTFVDFDDLDAVKAAINDDTRAVFCEAIANPGGYITDLDAISKISDAA
HHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEHHHHCCCCCCEECHHHHHHHHHCC
GIPLIVDNTSATPYLCRPIEHGATLVVHSTTKYLTGNGTVTGGCIVDSGKFDWSANDKFP
CCCEEEECCCCCCEEEEECCCCCEEEEECCEEEEECCCEEECCEEEECCCCCCCCCCCCC
SLSQPEPAYHGLKFHETFGPLAFTFHSIAIGLRDLGMTMNPQAAHYTLMGTETLSLRMDR
CCCCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEEHH
HVENAETITKWLEKDPRVDYVTYAGLPSSPYHDRVKRICPKGAGGLFTFAIKGGYEACIK
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
FVNALEVFSHVANLGDTRSIVTHSASTTHRQLTQEQQEAAGAGPGVVRISIGTEDADDLI
HHHHHHHHHHHHCCCCCHHHEECCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
ADLDQALAKATS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]