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The map label for this gene is yngF [H]

Identifier: 110679053

GI number: 110679053

Start: 1680402

End: 1681190

Strand: Direct

Name: yngF [H]

Synonym: RD1_1753

Alternate gene names: 110679053

Gene position: 1680402-1681190 (Clockwise)

Preceding gene: 110679050

Following gene: 110679057

Centisome position: 40.66

GC content: 64.13

Gene sequence:

>789_bases
ATGTCGCGCCTGCGCCTGAGCCACCACGATCATATCGCCCACGTCACCTTGACCCGGGCGGAAAAGAAAAACGCGATGGA
TGATGAGATGATTGATGCCATTATCGCGGCGGGCGAAGACGTTGCAGCCTCTTCTGCCCGCGCCGTGGTTTTGTCGGGGG
AAGGCAGTTGTTTTTGTGCGGGCATTGATATCGCCGGTCTGTCCGGGATGGTCGGGCAGGATATCGAAGCGCTTGTCATG
CCCCGCACCCACGGCATGGGGACGACGAACCGCTGGCAGGAGGTCGCGATGATCTGGCATCGCCTTGATATTCCTGTGAT
TGCCGCGCTGCATGGCGTCGTCTTTGGCGCTGGCCTGCAGCTGGCGTTGGGGGCGGATATTCGACTTGCGGCACCGGGCA
CGCAGGTGGCGGTGATGGAGATGAAGTGGGGTCTTATTCCGGACATGGGGGGCATGGTGCTGCTGCCGCGTCTGGTGCGC
GACGATGTGATGAAGCGGATGATCTACACCGCCGCCCCGATCCCGGCGGAGCAGGCGGCGGCCTGGGGGCTGGTCACGGA
GGTTGTCGCCGACCCGCTGGAGGCGGCGCAGGACCTGGCGACGCAGATCGCGGGCAATGGGCCGAACGCCATCCGGGCCG
CAAAGAGCCTGTGCGCCTATGCCCATACCGCTGCGCCGGCGGATGTCCTGAAGGAGGAATCGCGCCTGCAGGTGGCCTTG
TTGGGCAAGCCCGAGCAGATGGAAGTGATCGCCGCGCAATTTGCCAAACGCGCCCCGGATTTTGGCTAG

Upstream 100 bases:

>100_bases
TCAAGGTATGCGTCTTGAGCAAGCCCGCTTTGCAGTGACGTTGCGCAGGCAGTGAAATGTGTGTCAGGATTTCTACAGCT
ATCAGAAAAGGAGAGCCGCG

Downstream 100 bases:

>100_bases
CGCCGGGGGCGCATTTATCGGCTTGGCTGCGCTCCGCTCAGCCCAGTTCGGCCAGTCGCGCCAGCGCTTCTTTCAGCTTG
GCTTCTTCCTCTTCGCGCAG

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSRLRLSHHDHIAHVTLTRAEKKNAMDDEMIDAIIAAGEDVAASSARAVVLSGEGSCFCAGIDIAGLSGMVGQDIEALVM
PRTHGMGTTNRWQEVAMIWHRLDIPVIAALHGVVFGAGLQLALGADIRLAAPGTQVAVMEMKWGLIPDMGGMVLLPRLVR
DDVMKRMIYTAAPIPAEQAAAWGLVTEVVADPLEAAQDLATQIAGNGPNAIRAAKSLCAYAHTAAPADVLKEESRLQVAL
LGKPEQMEVIAAQFAKRAPDFG

Sequences:

>Translated_262_residues
MSRLRLSHHDHIAHVTLTRAEKKNAMDDEMIDAIIAAGEDVAASSARAVVLSGEGSCFCAGIDIAGLSGMVGQDIEALVM
PRTHGMGTTNRWQEVAMIWHRLDIPVIAALHGVVFGAGLQLALGADIRLAAPGTQVAVMEMKWGLIPDMGGMVLLPRLVR
DDVMKRMIYTAAPIPAEQAAAWGLVTEVVADPLEAAQDLATQIAGNGPNAIRAAKSLCAYAHTAAPADVLKEESRLQVAL
LGKPEQMEVIAAQFAKRAPDFG
>Mature_261_residues
SRLRLSHHDHIAHVTLTRAEKKNAMDDEMIDAIIAAGEDVAASSARAVVLSGEGSCFCAGIDIAGLSGMVGQDIEALVMP
RTHGMGTTNRWQEVAMIWHRLDIPVIAALHGVVFGAGLQLALGADIRLAAPGTQVAVMEMKWGLIPDMGGMVLLPRLVRD
DVMKRMIYTAAPIPAEQAAAWGLVTEVVADPLEAAQDLATQIAGNGPNAIRAAKSLCAYAHTAAPADVLKEESRLQVALL
GKPEQMEVIAAQFAKRAPDFG

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=29.4573643410853, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=28.0155642023346, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=198, Percent_Identity=31.8181818181818, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI31542718, Length=199, Percent_Identity=28.1407035175879, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4502327, Length=224, Percent_Identity=29.0178571428571, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=270, Percent_Identity=30.3703703703704, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI221142681, Length=221, Percent_Identity=32.1266968325792, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=30.5660377358491, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=24.8091603053435, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790281, Length=150, Percent_Identity=28.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=33.0232558139535, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=235, Percent_Identity=30.2127659574468, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=178, Percent_Identity=30.8988764044944, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=262, Percent_Identity=25.5725190839695, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=263, Percent_Identity=27.3764258555133, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=245, Percent_Identity=27.3469387755102, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=212, Percent_Identity=31.6037735849057, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=28.740157480315, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=28.740157480315, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27727; Mature: 27595

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLRLSHHDHIAHVTLTRAEKKNAMDDEMIDAIIAAGEDVAASSARAVVLSGEGSCFCA
CCCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEE
GIDIAGLSGMVGQDIEALVMPRTHGMGTTNRWQEVAMIWHRLDIPVIAALHGVVFGAGLQ
ECCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCE
LALGADIRLAAPGTQVAVMEMKWGLIPDMGGMVLLPRLVRDDVMKRMIYTAAPIPAEQAA
EEECCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AWGLVTEVVADPLEAAQDLATQIAGNGPNAIRAAKSLCAYAHTAAPADVLKEESRLQVAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEE
LGKPEQMEVIAAQFAKRAPDFG
CCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRLRLSHHDHIAHVTLTRAEKKNAMDDEMIDAIIAAGEDVAASSARAVVLSGEGSCFCA
CCCCCCCCCCEEEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEE
GIDIAGLSGMVGQDIEALVMPRTHGMGTTNRWQEVAMIWHRLDIPVIAALHGVVFGAGLQ
ECCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCE
LALGADIRLAAPGTQVAVMEMKWGLIPDMGGMVLLPRLVRDDVMKRMIYTAAPIPAEQAA
EEECCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AWGLVTEVVADPLEAAQDLATQIAGNGPNAIRAAKSLCAYAHTAAPADVLKEESRLQVAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEE
LGKPEQMEVIAAQFAKRAPDFG
CCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]