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The map label for this gene is tcmA [H]

Identifier: 110679008

GI number: 110679008

Start: 1640453

End: 1641601

Strand: Reverse

Name: tcmA [H]

Synonym: RD1_1707

Alternate gene names: 110679008

Gene position: 1641601-1640453 (Counterclockwise)

Preceding gene: 110679009

Following gene: 110679003

Centisome position: 39.72

GC content: 56.92

Gene sequence:

>1149_bases
ATGATTATCGGCCCGGTGGCGGCGCATGTCGCCTTCGTGCTGGAACTGACATTGGTGCCTCTGCTGCTGCCAGCGATGCA
GTCGCACCTTGGTTTGTCGGTTGGTGATCTGGCCTGGATCTTCAATTCTTACGGCATTGCAGTTGCAGTTGGTGTTTTGC
TGAGCGGATGGTGCGGCGACACCTTCAACCCGAGGAAAGTCTTCGGATACGGCGTCGCCCTCTTTGCAGTCGGCTCGCTT
TTGGTGGCCGCTTCAAACAGTTTCGAGATGCTCCTTTTCGGGCGTGCCGTTCAAGGTCTCGGCGCGGGTGTCTTTTCACC
TCTCCTTCCCCTGCTTCTGACGCGTGCCTCACCTTTGAAACCGGGGAGAATGTTGATCGTCTGGGGCAGTATTGCAGGCT
ATGTGTCCGCCCTTGCCCCGTTTTTTTACGGCAGCTTCTTCAGCAAATTCGGCTGGAACATCGCCTTTGTCTTCATTGCG
CTTGTGGCGCTTGTTGCATTGATACTGCTGAACAGGTTGCGCGACCCCGATGAGCACGCAGCAACACCGCCTGCTCCCAA
GGACTACACTCAGATTTTGCGCGCCCGCTATCTCTGGATCACCTTTGCCTATGTGTTTTGCACCTATGGCGCGATCACCT
ATTACCTGTTTCGGCTGCCGGTCTGGCTGTCGGACAATGACGTCAGGACGGCCAGTGTCGGCCTTGCCCTGACGGTTTTA
TGGCTGACATTTTCCGGGTTGAGCACCTTGCTGCGAAACATGGTGGACAGCCCACACATCCGCACGATCATGTTTGCCTC
GCCAATCCTTATCGTCGTTGGCTTTCTTCTTTCATATGGCAATGAAAATCTGTTTTTCATCGTGCTGTCGTGCGTCTTCG
TCGGTGCCGGCTTGGCCTGTAGCAATGCACCATCAACGCAGCTGATCCTGCGATTTGCTCCAAAGGGGCTCAGCGCGGCC
TCGACCAGTCTGGACATAACCTTCGCACGGCTCGGCGGAATTGCGATGGTCGCGCTGCTTGCAACTGCGGGCTTTGCCTT
TGCTTTCTACGCAACTGCCTTGTCGTGTTTCATCGCCGCGACCTGCGCGCTGATCGCCAGCAGGGGCCTCGGGTTTCCCC
CCAGCCCCGCTGCGCGCTCAACACTCTGA

Upstream 100 bases:

>100_bases
CGCCCACAAATGCAAATGCCTGAGCGGCGAGTTGGTATAAATGACATCGCACATACCGCAGATTGACAAGGCAAGCGCCG
CCGCCGTATCGCCGCGTGGC

Downstream 100 bases:

>100_bases
CCCAACGTCCGGGTGCGCCCCAGAAAAACAGGGGAGTAAGCAAAAAATGTTTCTCACCGATATTTGGCAACGATGCGCGT
TTCTCTGCGTAAGGCTGGGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MIIGPVAAHVAFVLELTLVPLLLPAMQSHLGLSVGDLAWIFNSYGIAVAVGVLLSGWCGDTFNPRKVFGYGVALFAVGSL
LVAASNSFEMLLFGRAVQGLGAGVFSPLLPLLLTRASPLKPGRMLIVWGSIAGYVSALAPFFYGSFFSKFGWNIAFVFIA
LVALVALILLNRLRDPDEHAATPPAPKDYTQILRARYLWITFAYVFCTYGAITYYLFRLPVWLSDNDVRTASVGLALTVL
WLTFSGLSTLLRNMVDSPHIRTIMFASPILIVVGFLLSYGNENLFFIVLSCVFVGAGLACSNAPSTQLILRFAPKGLSAA
STSLDITFARLGGIAMVALLATAGFAFAFYATALSCFIAATCALIASRGLGFPPSPAARSTL

Sequences:

>Translated_382_residues
MIIGPVAAHVAFVLELTLVPLLLPAMQSHLGLSVGDLAWIFNSYGIAVAVGVLLSGWCGDTFNPRKVFGYGVALFAVGSL
LVAASNSFEMLLFGRAVQGLGAGVFSPLLPLLLTRASPLKPGRMLIVWGSIAGYVSALAPFFYGSFFSKFGWNIAFVFIA
LVALVALILLNRLRDPDEHAATPPAPKDYTQILRARYLWITFAYVFCTYGAITYYLFRLPVWLSDNDVRTASVGLALTVL
WLTFSGLSTLLRNMVDSPHIRTIMFASPILIVVGFLLSYGNENLFFIVLSCVFVGAGLACSNAPSTQLILRFAPKGLSAA
STSLDITFARLGGIAMVALLATAGFAFAFYATALSCFIAATCALIASRGLGFPPSPAARSTL
>Mature_382_residues
MIIGPVAAHVAFVLELTLVPLLLPAMQSHLGLSVGDLAWIFNSYGIAVAVGVLLSGWCGDTFNPRKVFGYGVALFAVGSL
LVAASNSFEMLLFGRAVQGLGAGVFSPLLPLLLTRASPLKPGRMLIVWGSIAGYVSALAPFFYGSFFSKFGWNIAFVFIA
LVALVALILLNRLRDPDEHAATPPAPKDYTQILRARYLWITFAYVFCTYGAITYYLFRLPVWLSDNDVRTASVGLALTVL
WLTFSGLSTLLRNMVDSPHIRTIMFASPILIVVGFLLSYGNENLFFIVLSCVFVGAGLACSNAPSTQLILRFAPKGLSAA
STSLDITFARLGGIAMVALLATAGFAFAFYATALSCFIAATCALIASRGLGFPPSPAARSTL

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=163, Percent_Identity=30.0613496932515, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081983, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790195, Length=105, Percent_Identity=37.1428571428571, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40751; Mature: 40751

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGPVAAHVAFVLELTLVPLLLPAMQSHLGLSVGDLAWIFNSYGIAVAVGVLLSGWCGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
TFNPRKVFGYGVALFAVGSLLVAASNSFEMLLFGRAVQGLGAGVFSPLLPLLLTRASPLK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PGRMLIVWGSIAGYVSALAPFFYGSFFSKFGWNIAFVFIALVALVALILLNRLRDPDEHA
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ATPPAPKDYTQILRARYLWITFAYVFCTYGAITYYLFRLPVWLSDNDVRTASVGLALTVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHH
WLTFSGLSTLLRNMVDSPHIRTIMFASPILIVVGFLLSYGNENLFFIVLSCVFVGAGLAC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SNAPSTQLILRFAPKGLSAASTSLDITFARLGGIAMVALLATAGFAFAFYATALSCFIAA
CCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCALIASRGLGFPPSPAARSTL
HHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIIGPVAAHVAFVLELTLVPLLLPAMQSHLGLSVGDLAWIFNSYGIAVAVGVLLSGWCGD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
TFNPRKVFGYGVALFAVGSLLVAASNSFEMLLFGRAVQGLGAGVFSPLLPLLLTRASPLK
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
PGRMLIVWGSIAGYVSALAPFFYGSFFSKFGWNIAFVFIALVALVALILLNRLRDPDEHA
CCCEEEEECHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ATPPAPKDYTQILRARYLWITFAYVFCTYGAITYYLFRLPVWLSDNDVRTASVGLALTVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHH
WLTFSGLSTLLRNMVDSPHIRTIMFASPILIVVGFLLSYGNENLFFIVLSCVFVGAGLAC
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
SNAPSTQLILRFAPKGLSAASTSLDITFARLGGIAMVALLATAGFAFAFYATALSCFIAA
CCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TCALIASRGLGFPPSPAARSTL
HHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]