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The map label for this gene is mutB [H]

Identifier: 110678941

GI number: 110678941

Start: 1573051

End: 1575018

Strand: Reverse

Name: mutB [H]

Synonym: RD1_1635

Alternate gene names: 110678941

Gene position: 1575018-1573051 (Counterclockwise)

Preceding gene: 110678947

Following gene: 110678940

Centisome position: 38.11

GC content: 61.74

Gene sequence:

>1968_bases
ATGACGCACCCGCAGAAAGACCGACCCTGGCTGATCCGGACCTATGCCGGCCATTCCACCGCGAAAGCGTCAAACGAGCT
TTATCGCTCGAACCTTGCCAAGGGGCAGACCGGCTTGTCCGTGGCCTTCGATCTGCCGACCCAGACCGGCTACGACAGCG
ATCATATCCTCAGCCGGGGCGAAGTCGGGAAGGTCGGCGTGCCGGTTGGCCATCTGGGCGATATGCGCGCCTTGTTTGAC
CAGATCCCGCTGGATCAGATGAACACCTCGATGACGATCAATGCGACAGCACCATGGCTGTTGTCACTCTATATTGCCGT
GGCCGAGGAACAGGGCGCGGATATCTCAAAGCTGCAAGGCACGGTGCAGAATGACCTGATCAAGGAATATCTGAGCCGCG
GTACCTATATTTGCCCGCCCGCGCCCAGCCTCAAGATGATCGCGGATGTGGCCGAATACTGCTATACGCATGTGCCGAAA
TGGAACCCGATGAACGTCTGCTCCTACCACTTGCAGGAGGCGGGTGCGACGCCGGAACAGGAACTCGCCTTTGCGCTGGC
GACCGCGACCGCTGTGTTGGATGCGCTCAAACCCCGCGTCCCGGAAGAGGATTTCCCCGCACTTGTGGGTCGAATTTCGT
TCTTTGTGAACGCGGGCATCCGCTTTGTGACCGAGATGTGCAAGATGCGCGCCTTTGTCGATCTGTGGGACGAGATCTGC
CGCGACCGCTATGGTGTTGAGGACCCGAAATATCGCCGCTTCCGCTATGGTGTGCAGGTAAACTCACTTGGCCTGACCGA
GCAGCAGCCCGAAAACAATGTCTACCGCATCCTGATCGAAATGCTGGCGGTCACCCTGTCCAAAAAGGCACGCGCGCGCG
CCGTGCAATTGCCTGCGTGGAACGAAGCGCTTGGCCTGCCCCGTCCGTGGGATCAGCAGTGGTCGATGCGCATGCAGCAG
ATCATGGCCTATGAAACGGACCTTCTGGAGTTTGACGACCTGTTTGACGGCAATCCGGCGGTCGATGCCAAGGTCGAAGC
GCTCAAGGAAGGTGCGCGTCAGGAACTCGCGAACCTCGACAGTATGGGCGGCGCGATTGAAGCCATCGACTACATGAAAG
CACGCCTTGTGGACAGCAATGCCGATCGTCTGGGCCGTATTGAAGCGGGTGAGACCGTCGTGGTCGGTGTCAACAAATGG
CAGCAGGGCGAACCCTCGCCGCTGATGACGGGCGACGGGGGTATCATGGTCGTCGACCCGGCGGTCGAGGCGGAACAGAT
TTCGCGCCTTGATGCTTGGCGCGCCGCGCGGGACCCGGAGGCCGTCAAACAAGCCCTTGGTGATCTGCGCGCAGCGGCAA
AAGAGGGGCGCAATGTCATGCCCGCCTCGATTGCGGCGGCCAAGGCGGGTGTGACCACGGGCGAATGGGCCGCACAGATG
CGCGCCGTGCATGGTGAATACCGCGGCCCGACCGGCGTGTCCGGTGCGCGCTCCAACAGGACCGAGGGTCTGGATGATCT
GCGCGATGCGGTGGATGTGGTCAGTGACAAACTGGGCCGCCGCCTGAAGTTCCTTGTAGGCAAACCCGGGCTGGACGGTC
ACTCCAACGGGGCCGAACAAATCGCCGTGCGCGCCCGTGATTGCGGCATGGAAATCGCCTATGAGGGCATCCGCCTGACT
CCGGCGGAGATCGTAAACGCCGCGCTGGAGGACGAGGCGCATGTGGTCGGCCTGTCGATCCTGTCAGGCAGCCACATCCC
GCTGGTCGAAGACCTGATGACCCAAATGCGCGAGGCCGGGCTGGCCCATGTGCCTGTGATCGTCGGCGGTATCATCCCGG
ACGATGACGCCGAACGCCTGCGCGCGATGGGGGTTGCGCGGGTCTATACGCCCAAGGACTTTGAACTCAACACGATCATG
ATGGATATCGTCACGCTGGCGGACCCTCAGGCGGTGGCGGCACAGTAG

Upstream 100 bases:

>100_bases
GGCAAGTTGCTGCAGCGCGGCAAATTTGCATTGATATAAAATTCCGCATATGGCAGCTTGCACGTAAGAAAATTACTCAA
GCTGATTCAGGACGACTCAC

Downstream 100 bases:

>100_bases
CTGATGGCTTGCACAGGGCAGTGAGGTGCCGTTAACTGCGCCGAACTGCCAAAGGAGTCCTGCATGACCATCCATATCGG
TGCCCGCCCCGACCAGATTG

Product: methylmalonyl-CoA mutase

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MTHPQKDRPWLIRTYAGHSTAKASNELYRSNLAKGQTGLSVAFDLPTQTGYDSDHILSRGEVGKVGVPVGHLGDMRALFD
QIPLDQMNTSMTINATAPWLLSLYIAVAEEQGADISKLQGTVQNDLIKEYLSRGTYICPPAPSLKMIADVAEYCYTHVPK
WNPMNVCSYHLQEAGATPEQELAFALATATAVLDALKPRVPEEDFPALVGRISFFVNAGIRFVTEMCKMRAFVDLWDEIC
RDRYGVEDPKYRRFRYGVQVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAWNEALGLPRPWDQQWSMRMQQ
IMAYETDLLEFDDLFDGNPAVDAKVEALKEGARQELANLDSMGGAIEAIDYMKARLVDSNADRLGRIEAGETVVVGVNKW
QQGEPSPLMTGDGGIMVVDPAVEAEQISRLDAWRAARDPEAVKQALGDLRAAAKEGRNVMPASIAAAKAGVTTGEWAAQM
RAVHGEYRGPTGVSGARSNRTEGLDDLRDAVDVVSDKLGRRLKFLVGKPGLDGHSNGAEQIAVRARDCGMEIAYEGIRLT
PAEIVNAALEDEAHVVGLSILSGSHIPLVEDLMTQMREAGLAHVPVIVGGIIPDDDAERLRAMGVARVYTPKDFELNTIM
MDIVTLADPQAVAAQ

Sequences:

>Translated_655_residues
MTHPQKDRPWLIRTYAGHSTAKASNELYRSNLAKGQTGLSVAFDLPTQTGYDSDHILSRGEVGKVGVPVGHLGDMRALFD
QIPLDQMNTSMTINATAPWLLSLYIAVAEEQGADISKLQGTVQNDLIKEYLSRGTYICPPAPSLKMIADVAEYCYTHVPK
WNPMNVCSYHLQEAGATPEQELAFALATATAVLDALKPRVPEEDFPALVGRISFFVNAGIRFVTEMCKMRAFVDLWDEIC
RDRYGVEDPKYRRFRYGVQVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAWNEALGLPRPWDQQWSMRMQQ
IMAYETDLLEFDDLFDGNPAVDAKVEALKEGARQELANLDSMGGAIEAIDYMKARLVDSNADRLGRIEAGETVVVGVNKW
QQGEPSPLMTGDGGIMVVDPAVEAEQISRLDAWRAARDPEAVKQALGDLRAAAKEGRNVMPASIAAAKAGVTTGEWAAQM
RAVHGEYRGPTGVSGARSNRTEGLDDLRDAVDVVSDKLGRRLKFLVGKPGLDGHSNGAEQIAVRARDCGMEIAYEGIRLT
PAEIVNAALEDEAHVVGLSILSGSHIPLVEDLMTQMREAGLAHVPVIVGGIIPDDDAERLRAMGVARVYTPKDFELNTIM
MDIVTLADPQAVAAQ
>Mature_654_residues
THPQKDRPWLIRTYAGHSTAKASNELYRSNLAKGQTGLSVAFDLPTQTGYDSDHILSRGEVGKVGVPVGHLGDMRALFDQ
IPLDQMNTSMTINATAPWLLSLYIAVAEEQGADISKLQGTVQNDLIKEYLSRGTYICPPAPSLKMIADVAEYCYTHVPKW
NPMNVCSYHLQEAGATPEQELAFALATATAVLDALKPRVPEEDFPALVGRISFFVNAGIRFVTEMCKMRAFVDLWDEICR
DRYGVEDPKYRRFRYGVQVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAWNEALGLPRPWDQQWSMRMQQI
MAYETDLLEFDDLFDGNPAVDAKVEALKEGARQELANLDSMGGAIEAIDYMKARLVDSNADRLGRIEAGETVVVGVNKWQ
QGEPSPLMTGDGGIMVVDPAVEAEQISRLDAWRAARDPEAVKQALGDLRAAAKEGRNVMPASIAAAKAGVTTGEWAAQMR
AVHGEYRGPTGVSGARSNRTEGLDDLRDAVDVVSDKLGRRLKFLVGKPGLDGHSNGAEQIAVRARDCGMEIAYEGIRLTP
AEIVNAALEDEAHVVGLSILSGSHIPLVEDLMTQMREAGLAHVPVIVGGIIPDDDAERLRAMGVARVYTPKDFELNTIMM
DIVTLADPQAVAAQ

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=632, Percent_Identity=34.6518987341772, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1789284, Length=651, Percent_Identity=37.0199692780338, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25144500, Length=641, Percent_Identity=33.8533541341654, Blast_Score=367, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 71665; Mature: 71534

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPQKDRPWLIRTYAGHSTAKASNELYRSNLAKGQTGLSVAFDLPTQTGYDSDHILSRG
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCCCC
EVGKVGVPVGHLGDMRALFDQIPLDQMNTSMTINATAPWLLSLYIAVAEEQGADISKLQG
CCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHH
TVQNDLIKEYLSRGTYICPPAPSLKMIADVAEYCYTHVPKWNPMNVCSYHLQEAGATPEQ
HHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
ELAFALATATAVLDALKPRVPEEDFPALVGRISFFVNAGIRFVTEMCKMRAFVDLWDEIC
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRYGVEDPKYRRFRYGVQVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAW
HHHCCCCCCCHHHEEECEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCC
NEALGLPRPWDQQWSMRMQQIMAYETDLLEFDDLFDGNPAVDAKVEALKEGARQELANLD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SMGGAIEAIDYMKARLVDSNADRLGRIEAGETVVVGVNKWQQGEPSPLMTGDGGIMVVDP
HCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECC
AVEAEQISRLDAWRAARDPEAVKQALGDLRAAAKEGRNVMPASIAAAKAGVTTGEWAAQM
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHH
RAVHGEYRGPTGVSGARSNRTEGLDDLRDAVDVVSDKLGRRLKFLVGKPGLDGHSNGAEQ
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IAVRARDCGMEIAYEGIRLTPAEIVNAALEDEAHVVGLSILSGSHIPLVEDLMTQMREAG
HHHHHHHCCCCEECCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
LAHVPVIVGGIIPDDDAERLRAMGVARVYTPKDFELNTIMMDIVTLADPQAVAAQ
CCCCCEEEECCCCCCHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
THPQKDRPWLIRTYAGHSTAKASNELYRSNLAKGQTGLSVAFDLPTQTGYDSDHILSRG
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHCCCC
EVGKVGVPVGHLGDMRALFDQIPLDQMNTSMTINATAPWLLSLYIAVAEEQGADISKLQG
CCCCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHH
TVQNDLIKEYLSRGTYICPPAPSLKMIADVAEYCYTHVPKWNPMNVCSYHLQEAGATPEQ
HHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHH
ELAFALATATAVLDALKPRVPEEDFPALVGRISFFVNAGIRFVTEMCKMRAFVDLWDEIC
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDRYGVEDPKYRRFRYGVQVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAW
HHHCCCCCCCHHHEEECEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCC
NEALGLPRPWDQQWSMRMQQIMAYETDLLEFDDLFDGNPAVDAKVEALKEGARQELANLD
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SMGGAIEAIDYMKARLVDSNADRLGRIEAGETVVVGVNKWQQGEPSPLMTGDGGIMVVDP
HCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECC
AVEAEQISRLDAWRAARDPEAVKQALGDLRAAAKEGRNVMPASIAAAKAGVTTGEWAAQM
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHH
RAVHGEYRGPTGVSGARSNRTEGLDDLRDAVDVVSDKLGRRLKFLVGKPGLDGHSNGAEQ
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IAVRARDCGMEIAYEGIRLTPAEIVNAALEDEAHVVGLSILSGSHIPLVEDLMTQMREAG
HHHHHHHCCCCEECCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCC
LAHVPVIVGGIIPDDDAERLRAMGVARVYTPKDFELNTIMMDIVTLADPQAVAAQ
CCCCCEEEECCCCCCHHHHHHHHCHHEEECCCCCCHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]