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The map label for this gene is citE [H]

Identifier: 110678926

GI number: 110678926

Start: 1562307

End: 1563164

Strand: Direct

Name: citE [H]

Synonym: RD1_1620

Alternate gene names: 110678926

Gene position: 1562307-1563164 (Clockwise)

Preceding gene: 110678924

Following gene: 110678927

Centisome position: 37.8

GC content: 60.84

Gene sequence:

>858_bases
ATGTTACATGCCAACCGACCCCTGCGGTCCGTTTTATATTTGCCTGCGTCCAACGCGCGCGCCTTGGTCAAGGCGCGCAG
TCTGGAAGTGGATGCGATTATTTTTGATCTCGAAGATGCGGTCGCACCTGATGAAAAGGCAGGCGCGCGCAATACACTTG
CGCAGGCGCTGGACGAGGGCGGCTATGGCGCGCGCCTGAAAATCGTGCGGGTCAATGCGCTGGACACACCCTGGGGTGTT
GCGGATGCCAAGTCGGCGGCTGACATGGGCGCGGATGCGGTACTGCTGCCAAAGGTTGACGGGCCCGCGCAGCTTGACGC
GCTTGCTGCACTCACTGGTGATTTGCCGCTCTGGGCGATGATGGAGACGCCGCTTGGTATGCTGAATGCCGCCGCAATCG
CGGCCCACGGGCGCTGTGCCGGGTTGGTCATGGGCACCAATGATCTGGCCAAGGATATGGGCACGCGGTTCCGTCCGGAT
CGTTTGCCGATGATGGCCGGGCTGGGTCTGTGCCTCCTGGCCGCCAAGGCGCATGGCGTCGCGATTGTGGACGGTGTGTA
TAATGCCTTCAAGGATGATGAGGGGCTGAAAGCCGAATGTGAACAGGGGCGCGATATGGGGTTTGATGGAAAAACGCTGA
TCCACCCCGCGCAGGTCGCCATCGCCAACGCAGCCTTTGCACCGACCGAAGAAGAGGTTGAGCTGGCCCGCCGCCAGATC
GACGCCTATGAGGCAGCAATTGCCGAAGGGCAGGCGGTTGCGGTGGTGGACAATCGGATCGTTGAAAATCTGCATGTTGC
AACTGCGCGCGAAACGTTGGCAAAAAGGGACGCAATAGCAACCCTGAACGCGAGGTAG

Upstream 100 bases:

>100_bases
TCCGAAAATAATACGAGTTTGCGCAAGTTTATTAGGCACGCCGATGCCCCGGTCCTTTGGCTCAGGCTCCCGGCGTCGCG
GTCACGATCGGAGCTGTTTC

Downstream 100 bases:

>100_bases
CCACATGTTACTCCTGATCGCCGGCCTGATCCTCTGGATCGCAGCGCATTACTTCAAACGTCTGATGCCGCAACAGCGCG
CGGCGATGGGGAATGCGGGC

Product: citrate lyase, beta subunit, putative

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MLHANRPLRSVLYLPASNARALVKARSLEVDAIIFDLEDAVAPDEKAGARNTLAQALDEGGYGARLKIVRVNALDTPWGV
ADAKSAADMGADAVLLPKVDGPAQLDALAALTGDLPLWAMMETPLGMLNAAAIAAHGRCAGLVMGTNDLAKDMGTRFRPD
RLPMMAGLGLCLLAAKAHGVAIVDGVYNAFKDDEGLKAECEQGRDMGFDGKTLIHPAQVAIANAAFAPTEEEVELARRQI
DAYEAAIAEGQAVAVVDNRIVENLHVATARETLAKRDAIATLNAR

Sequences:

>Translated_285_residues
MLHANRPLRSVLYLPASNARALVKARSLEVDAIIFDLEDAVAPDEKAGARNTLAQALDEGGYGARLKIVRVNALDTPWGV
ADAKSAADMGADAVLLPKVDGPAQLDALAALTGDLPLWAMMETPLGMLNAAAIAAHGRCAGLVMGTNDLAKDMGTRFRPD
RLPMMAGLGLCLLAAKAHGVAIVDGVYNAFKDDEGLKAECEQGRDMGFDGKTLIHPAQVAIANAAFAPTEEEVELARRQI
DAYEAAIAEGQAVAVVDNRIVENLHVATARETLAKRDAIATLNAR
>Mature_285_residues
MLHANRPLRSVLYLPASNARALVKARSLEVDAIIFDLEDAVAPDEKAGARNTLAQALDEGGYGARLKIVRVNALDTPWGV
ADAKSAADMGADAVLLPKVDGPAQLDALAALTGDLPLWAMMETPLGMLNAAAIAAHGRCAGLVMGTNDLAKDMGTRFRPD
RLPMMAGLGLCLLAAKAHGVAIVDGVYNAFKDDEGLKAECEQGRDMGFDGKTLIHPAQVAIANAAFAPTEEEVELARRQI
DAYEAAIAEGQAVAVVDNRIVENLHVATARETLAKRDAIATLNAR

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=293, Percent_Identity=26.962457337884, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI87081764, Length=283, Percent_Identity=34.6289752650177, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17557458, Length=273, Percent_Identity=29.3040293040293, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30010; Mature: 30010

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHANRPLRSVLYLPASNARALVKARSLEVDAIIFDLEDAVAPDEKAGARNTLAQALDEG
CCCCCCCHHHEEEECCCCCHHHHHHHCCEEEEEEEECHHHCCCCCCCCHHHHHHHHHHCC
GYGARLKIVRVNALDTPWGVADAKSAADMGADAVLLPKVDGPAQLDALAALTGDLPLWAM
CCCEEEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEHH
METPLGMLNAAAIAAHGRCAGLVMGTNDLAKDMGTRFRPDRLPMMAGLGLCLLAAKAHGV
HHCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCE
AIVDGVYNAFKDDEGLKAECEQGRDMGFDGKTLIHPAQVAIANAAFAPTEEEVELARRQI
EEEHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECHHHHHEECCCCCCCHHHHHHHHHHH
DAYEAAIAEGQAVAVVDNRIVENLHVATARETLAKRDAIATLNAR
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MLHANRPLRSVLYLPASNARALVKARSLEVDAIIFDLEDAVAPDEKAGARNTLAQALDEG
CCCCCCCHHHEEEECCCCCHHHHHHHCCEEEEEEEECHHHCCCCCCCCHHHHHHHHHHCC
GYGARLKIVRVNALDTPWGVADAKSAADMGADAVLLPKVDGPAQLDALAALTGDLPLWAM
CCCEEEEEEEEECCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCEEHH
METPLGMLNAAAIAAHGRCAGLVMGTNDLAKDMGTRFRPDRLPMMAGLGLCLLAAKAHGV
HHCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCE
AIVDGVYNAFKDDEGLKAECEQGRDMGFDGKTLIHPAQVAIANAAFAPTEEEVELARRQI
EEEHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEECHHHHHEECCCCCCCHHHHHHHHHHH
DAYEAAIAEGQAVAVVDNRIVENLHVATARETLAKRDAIATLNAR
HHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA