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The map label for this gene is purR [H]

Identifier: 110678799

GI number: 110678799

Start: 1411309

End: 1412340

Strand: Reverse

Name: purR [H]

Synonym: RD1_1487

Alternate gene names: 110678799

Gene position: 1412340-1411309 (Counterclockwise)

Preceding gene: 110678800

Following gene: 110678798

Centisome position: 34.17

GC content: 53.1

Gene sequence:

>1032_bases
ATGGGACACTCAAAAATTCGCAATATGGAAGAATTTGCCGCGCTCAGCGGCATTTCCCGCCCAACCGTTTCAAAGTACTT
CAATGATCCGGAAAGCGTACGCTCCTCGACACGTGCGAAGATCGAGGCGTCCCTCAAGCAATACGACTACCAGCCCAATG
TTTTCGCCATAAACCAGAACAGGCGAACGACCAAGAACATAGGAATCGTCGTACCAAACCTTGCAGACCCTTTTTTTTCG
GAGATTGGCAGGCGGGTTGAGCTTGCCTGCATCGCAGCTGGTTATAGCCCGATTTTACAAAGTTCGCACGGCAGCCCGGA
GATGGAGCGGGACAATCTGAATTCCTTGCGGTCCCTCAAACCGGCGGGGGTTCTGCTTGCACCTTTCGGTCGCATGTCTG
ACGCAGATGCCGTCAAGAGTTTTGACGATGACTTTCCGCTGGTTCTGTTTGACGCCAATATGGATGGCATCGGTCAGGCC
TTTGTGGGTACGGACAACTATCAAAGTGTTGATTTAATGGTGGAATACCTTTGTCGTAGTGGCGAAGCACCCTGTTTTTT
TGAAATGAAATATCCCTCTAACCCCAACGCAAACAAGCGTCGGCAGGCCTACATTCAAAGTATGGAGCGCCACGGGCATG
CGCCGCGCATCTTTCAGGCTGATGGCGAGGGCTGGAACTTTGAGGAGATCGGACGCACAGAGGGCGGGCGGTTTTTGTCT
GCAAAAGAATTCAGCACGAACACGATCCTGTGCAGCAACGACCGTCTGGCAATTGGGATGCTATCCGCCGCCTATAGCCT
TGGGTTGCGGGTGGGCCATGGTGCGGATTGCGATCTGCGCATCGCCGGGCATGATGACCATCCCTTCTCACAATTCACAT
GCCCGCCGCTCACGACAATCGCACAAGACTACGAAACCATCGCGAATCGCGCCGTCAGCGAACTTTTTGGCATGTTGGAA
AATAATGACCGGCTCTCGGATCGCACAGAAACGACGTTTTATGGTCGCCTGATCATGAGGGATTCCGCCTAA

Upstream 100 bases:

>100_bases
CGTTTACGGCCTCTGGTCACGAATGCCCTTGACGGCTTACAGAGGGTTTTTGTACGGTAAAAAAGTTATCGCGCGTAATT
TTTTACCGTCAGGGGTAAAA

Downstream 100 bases:

>100_bases
GCACTTGTTTTGACATGCGCCTCGCCTTTTATTTACGCGCGATAACTTTTAATTGACTTCGCGCCATCTCCCCTGTTAGT
GATTAACCATCATCCAACCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MGHSKIRNMEEFAALSGISRPTVSKYFNDPESVRSSTRAKIEASLKQYDYQPNVFAINQNRRTTKNIGIVVPNLADPFFS
EIGRRVELACIAAGYSPILQSSHGSPEMERDNLNSLRSLKPAGVLLAPFGRMSDADAVKSFDDDFPLVLFDANMDGIGQA
FVGTDNYQSVDLMVEYLCRSGEAPCFFEMKYPSNPNANKRRQAYIQSMERHGHAPRIFQADGEGWNFEEIGRTEGGRFLS
AKEFSTNTILCSNDRLAIGMLSAAYSLGLRVGHGADCDLRIAGHDDHPFSQFTCPPLTTIAQDYETIANRAVSELFGMLE
NNDRLSDRTETTFYGRLIMRDSA

Sequences:

>Translated_343_residues
MGHSKIRNMEEFAALSGISRPTVSKYFNDPESVRSSTRAKIEASLKQYDYQPNVFAINQNRRTTKNIGIVVPNLADPFFS
EIGRRVELACIAAGYSPILQSSHGSPEMERDNLNSLRSLKPAGVLLAPFGRMSDADAVKSFDDDFPLVLFDANMDGIGQA
FVGTDNYQSVDLMVEYLCRSGEAPCFFEMKYPSNPNANKRRQAYIQSMERHGHAPRIFQADGEGWNFEEIGRTEGGRFLS
AKEFSTNTILCSNDRLAIGMLSAAYSLGLRVGHGADCDLRIAGHDDHPFSQFTCPPLTTIAQDYETIANRAVSELFGMLE
NNDRLSDRTETTFYGRLIMRDSA
>Mature_342_residues
GHSKIRNMEEFAALSGISRPTVSKYFNDPESVRSSTRAKIEASLKQYDYQPNVFAINQNRRTTKNIGIVVPNLADPFFSE
IGRRVELACIAAGYSPILQSSHGSPEMERDNLNSLRSLKPAGVLLAPFGRMSDADAVKSFDDDFPLVLFDANMDGIGQAF
VGTDNYQSVDLMVEYLCRSGEAPCFFEMKYPSNPNANKRRQAYIQSMERHGHAPRIFQADGEGWNFEEIGRTEGGRFLSA
KEFSTNTILCSNDRLAIGMLSAAYSLGLRVGHGADCDLRIAGHDDHPFSQFTCPPLTTIAQDYETIANRAVSELFGMLEN
NDRLSDRTETTFYGRLIMRDSA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=311, Percent_Identity=27.6527331189711, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790369, Length=317, Percent_Identity=27.1293375394322, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=25.4777070063694, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=24.1176470588235, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789202, Length=312, Percent_Identity=23.7179487179487, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=23.5294117647059, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786540, Length=315, Percent_Identity=25.0793650793651, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=23.5668789808917, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=20.7547169811321, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=22.3241590214067, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38176; Mature: 38045

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHSKIRNMEEFAALSGISRPTVSKYFNDPESVRSSTRAKIEASLKQYDYQPNVFAINQN
CCHHHHCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRTTKNIGIVVPNLADPFFSEIGRRVELACIAAGYSPILQSSHGSPEMERDNLNSLRSLK
CCCCCCCCEEECCCCCHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCCCHHHHHHHHHCC
PAGVLLAPFGRMSDADAVKSFDDDFPLVLFDANMDGIGQAFVGTDNYQSVDLMVEYLCRS
CCCEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHC
GEAPCFFEMKYPSNPNANKRRQAYIQSMERHGHAPRIFQADGEGWNFEEIGRTEGGRFLS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCCEEE
AKEFSTNTILCSNDRLAIGMLSAAYSLGLRVGHGADCDLRIAGHDDHPFSQFTCPPLTTI
ECCCCCCEEEECCCCEEEHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCCCCCCCHHHH
AQDYETIANRAVSELFGMLENNDRLSDRTETTFYGRLIMRDSA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
GHSKIRNMEEFAALSGISRPTVSKYFNDPESVRSSTRAKIEASLKQYDYQPNVFAINQN
CHHHHCCHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
RRTTKNIGIVVPNLADPFFSEIGRRVELACIAAGYSPILQSSHGSPEMERDNLNSLRSLK
CCCCCCCCEEECCCCCHHHHHHCCEEEEEEEECCCHHHHHCCCCCCCCCHHHHHHHHHCC
PAGVLLAPFGRMSDADAVKSFDDDFPLVLFDANMDGIGQAFVGTDNYQSVDLMVEYLCRS
CCCEEEECCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHC
GEAPCFFEMKYPSNPNANKRRQAYIQSMERHGHAPRIFQADGEGWNFEEIGRTEGGRFLS
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCCCCCCCEEE
AKEFSTNTILCSNDRLAIGMLSAAYSLGLRVGHGADCDLRIAGHDDHPFSQFTCPPLTTI
ECCCCCCEEEECCCCEEEHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCCCCCCCHHHH
AQDYETIANRAVSELFGMLENNDRLSDRTETTFYGRLIMRDSA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA