Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 110678790

GI number: 110678790

Start: 1402819

End: 1403808

Strand: Reverse

Name: yiaO [H]

Synonym: RD1_1478

Alternate gene names: 110678790

Gene position: 1403808-1402819 (Counterclockwise)

Preceding gene: 110678792

Following gene: 110678789

Centisome position: 33.97

GC content: 57.37

Gene sequence:

>990_bases
ATGAAAACGAAATTTCTAACGACAGTCGCGGTGGCGGCGGCAATGGGCCTCGGGGCGATGGCACATGCGACCGAGCTTCG
CCTGTCTCACCAGTGGTCCAACCAGGACATTCGCCATCAGGTCGCGCAAATGGTTGCCGATGGTGTCGCTGAGGCTGATG
TGGATCTGGAGATCAAGATTTTCCCTTCCAGATCGCTGTTCAAACCCCGCGAACAATACCGCCCCCTGACGCGCGGGCAA
TTGGACATGACCGTTCTGCCATTGAGCTACGCAGGCGGTCAGCAGCCAGCGTTCAACCTGACGCTGATGCCGGGCCTTGT
GAAAAACCATGACCACGCCGCCCGTTTGAGCGCATCGCCCTATATGGCCGCCCTTGAGGAAAAGATGGCAGAGGATGATG
TCATGGTGCTGGTCCACGGCTATCTGGCCGGTGGTTTCGTGGGCAAAGACAAATGCATCACCAGCCCTGCCGATGTGGAT
GGTTTGCAGACACGTGCTGCGGGCAAGGCCTTCGAGCAGATGCTGGCGGGCGCGAATGCGTCGATCACATCCATGGCGTC
TTCAGAGATCTACAACGCCATGCAAACCGGTATTCTGAATGCCGCGAACACATCCTCCTCGTCCTTCGTGTCCTATCGGA
TTTACGAGCAGGTCTCCTGCTACACGCCTGCGACGGAGGATTTCGCATTGTGGTTCATGTATCAGCCGCTTCTGATGAAC
AAATCCACCTTTGAAGGCCTGTCGGAAGAACAGCAAAACGCCCTGCTGGCGGCTGCGGAAAAAGCCGAGGCCTTCTATCT
GGAAGAAGCGAAAAAGCAGGATGCCGCCTCGGTAAAGGTCTTTGAAGACGCCGGCGTTGAAATCGCCGCGATGACAGCGG
AAGACTTCGAAGAATGGCGCGCACTGGCGCAAGAGACCTCTTACAAGGCCTTCGTGGAGGATGTGCCAGACGGGCAGGCC
TTGCTCGATATGGCGCTGGCCGTCGAGTAA

Upstream 100 bases:

>100_bases
TTTATTATATTGTATACAAGAATGTTGACTGGGAATGCCAAATGAGCAATGGTGGATTACTGCCGACCCTAGGGAGTCGG
CTCACCTTTGGGAGGAAACC

Downstream 100 bases:

>100_bases
CCCGGCATAGGGCGACCGCGCAATGCAGCGGTCGCCGCAATGCTCCGAAACTCTTACTTCTAAAAGGAAAATCGACATGG
CTGGTCAAAGCTCAGCCGTC

Product: C4-dicarboxylate transport system C4-dicarboxylate-binding protein, putative

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKTKFLTTVAVAAAMGLGAMAHATELRLSHQWSNQDIRHQVAQMVADGVAEADVDLEIKIFPSRSLFKPREQYRPLTRGQ
LDMTVLPLSYAGGQQPAFNLTLMPGLVKNHDHAARLSASPYMAALEEKMAEDDVMVLVHGYLAGGFVGKDKCITSPADVD
GLQTRAAGKAFEQMLAGANASITSMASSEIYNAMQTGILNAANTSSSSFVSYRIYEQVSCYTPATEDFALWFMYQPLLMN
KSTFEGLSEEQQNALLAAAEKAEAFYLEEAKKQDAASVKVFEDAGVEIAAMTAEDFEEWRALAQETSYKAFVEDVPDGQA
LLDMALAVE

Sequences:

>Translated_329_residues
MKTKFLTTVAVAAAMGLGAMAHATELRLSHQWSNQDIRHQVAQMVADGVAEADVDLEIKIFPSRSLFKPREQYRPLTRGQ
LDMTVLPLSYAGGQQPAFNLTLMPGLVKNHDHAARLSASPYMAALEEKMAEDDVMVLVHGYLAGGFVGKDKCITSPADVD
GLQTRAAGKAFEQMLAGANASITSMASSEIYNAMQTGILNAANTSSSSFVSYRIYEQVSCYTPATEDFALWFMYQPLLMN
KSTFEGLSEEQQNALLAAAEKAEAFYLEEAKKQDAASVKVFEDAGVEIAAMTAEDFEEWRALAQETSYKAFVEDVPDGQA
LLDMALAVE
>Mature_329_residues
MKTKFLTTVAVAAAMGLGAMAHATELRLSHQWSNQDIRHQVAQMVADGVAEADVDLEIKIFPSRSLFKPREQYRPLTRGQ
LDMTVLPLSYAGGQQPAFNLTLMPGLVKNHDHAARLSASPYMAALEEKMAEDDVMVLVHGYLAGGFVGKDKCITSPADVD
GLQTRAAGKAFEQMLAGANASITSMASSEIYNAMQTGILNAANTSSSSFVSYRIYEQVSCYTPATEDFALWFMYQPLLMN
KSTFEGLSEEQQNALLAAAEKAEAFYLEEAKKQDAASVKVFEDAGVEIAAMTAEDFEEWRALAQETSYKAFVEDVPDGQA
LLDMALAVE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=328, Percent_Identity=26.219512195122, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35841; Mature: 35841

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKFLTTVAVAAAMGLGAMAHATELRLSHQWSNQDIRHQVAQMVADGVAEADVDLEIKI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
FPSRSLFKPREQYRPLTRGQLDMTVLPLSYAGGQQPAFNLTLMPGLVKNHDHAARLSASP
ECCCCCCCCHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCHHHCCCCH
YMAALEEKMAEDDVMVLVHGYLAGGFVGKDKCITSPADVDGLQTRAAGKAFEQMLAGANA
HHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
SITSMASSEIYNAMQTGILNAANTSSSSFVSYRIYEQVSCYTPATEDFALWFMYQPLLMN
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHCCCCCCCCHHEEHEEHHHHHCC
KSTFEGLSEEQQNALLAAAEKAEAFYLEEAKKQDAASVKVFEDAGVEIAAMTAEDFEEWR
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHHHHHHH
ALAQETSYKAFVEDVPDGQALLDMALAVE
HHHHHCCHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKTKFLTTVAVAAAMGLGAMAHATELRLSHQWSNQDIRHQVAQMVADGVAEADVDLEIKI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEE
FPSRSLFKPREQYRPLTRGQLDMTVLPLSYAGGQQPAFNLTLMPGLVKNHDHAARLSASP
ECCCCCCCCHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCHHCCCCCHHHCCCCH
YMAALEEKMAEDDVMVLVHGYLAGGFVGKDKCITSPADVDGLQTRAAGKAFEQMLAGANA
HHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
SITSMASSEIYNAMQTGILNAANTSSSSFVSYRIYEQVSCYTPATEDFALWFMYQPLLMN
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHHCCCCCCCCHHEEHEEHHHHHCC
KSTFEGLSEEQQNALLAAAEKAEAFYLEEAKKQDAASVKVFEDAGVEIAAMTAEDFEEWR
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEECHHHHHHHH
ALAQETSYKAFVEDVPDGQALLDMALAVE
HHHHHCCHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]