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The map label for this gene is def [H]

Identifier: 110678659

GI number: 110678659

Start: 1287913

End: 1288410

Strand: Direct

Name: def [H]

Synonym: RD1_1338

Alternate gene names: 110678659

Gene position: 1287913-1288410 (Clockwise)

Preceding gene: 110678658

Following gene: 110678660

Centisome position: 31.16

GC content: 59.44

Gene sequence:

>498_bases
ATGAGCGTACGCGCTTACCTTAAATGGCCAGACGCGCGGCTGCGCACTGCCGCCGCCGATGTAGCGGAGATCACGCCGGA
GGTGCTGGGCATCTGGGATGATATGATCGACACGATGGATGCCATGCCGGGGGTGGGGCTGGCGGCGCCGCAGATCGGCG
TGATGTTGCGGCTGGCCGTCGTGGATGCATCAGACACGCGCGACCGGCGCATTCGTCTCGCCAATCCGGAGATTATCGAG
GCGTCTGCCATCATGAATGATCATGAAGAGGCGAGTCCGAATTTACCCGGTGTATTTGCCAAAATCCGGCGTCCGCGCGG
CGTGACCGTGCGTTTCATGGATGAAACCGGCACCTACGTGCAGCGCGATTTTGTCGGGCTTGAGGCGACGAGCGTGCAGC
ATCAGATCGACCATTTGCAGGGAAAGATGTATTTCGACAATCTCAGCAAGGTGAAACGGGATATGCTGCTGCGCAAGGCG
CGTAAACTGAACGGCTAG

Upstream 100 bases:

>100_bases
ACCATTTGGATGGCAAGCTGTTTATTGACTACCTCAGGCCGCTGCGCCGCCAGATGATTACCCGCAAGATGGTCAAGCTG
AAACGGGAACTGGCCCGCAC

Downstream 100 bases:

>100_bases
TGCCCTCTGGAGGATAAGATGCGTTTGGTTTTCATGGGAACACCCGATTTTTCGGTCCCTGTGCTTGAGGCACTGGTGGC
GGCAGGACATGAGATCGCCT

Product: peptide deformylase, putative

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSVRAYLKWPDARLRTAAADVAEITPEVLGIWDDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDTRDRRIRLANPEIIE
ASAIMNDHEEASPNLPGVFAKIRRPRGVTVRFMDETGTYVQRDFVGLEATSVQHQIDHLQGKMYFDNLSKVKRDMLLRKA
RKLNG

Sequences:

>Translated_165_residues
MSVRAYLKWPDARLRTAAADVAEITPEVLGIWDDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDTRDRRIRLANPEIIE
ASAIMNDHEEASPNLPGVFAKIRRPRGVTVRFMDETGTYVQRDFVGLEATSVQHQIDHLQGKMYFDNLSKVKRDMLLRKA
RKLNG
>Mature_164_residues
SVRAYLKWPDARLRTAAADVAEITPEVLGIWDDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDTRDRRIRLANPEIIEA
SAIMNDHEEASPNLPGVFAKIRRPRGVTVRFMDETGTYVQRDFVGLEATSVQHQIDHLQGKMYFDNLSKVKRDMLLRKAR
KLNG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=39.6341463414634, Blast_Score=115, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645726, Length=163, Percent_Identity=28.8343558282209, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24645728, Length=138, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18490; Mature: 18358

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRAYLKWPDARLRTAAADVAEITPEVLGIWDDMIDTMDAMPGVGLAAPQIGVMLRLAV
CCCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEE
VDASDTRDRRIRLANPEIIEASAIMNDHEEASPNLPGVFAKIRRPRGVTVRFMDETGTYV
ECCCCCCCCEEEECCCCEEEHHHHHCCCHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCH
QRDFVGLEATSVQHQIDHLQGKMYFDNLSKVKRDMLLRKARKLNG
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRAYLKWPDARLRTAAADVAEITPEVLGIWDDMIDTMDAMPGVGLAAPQIGVMLRLAV
CCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEE
VDASDTRDRRIRLANPEIIEASAIMNDHEEASPNLPGVFAKIRRPRGVTVRFMDETGTYV
ECCCCCCCCEEEECCCCEEEHHHHHCCCHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCH
QRDFVGLEATSVQHQIDHLQGKMYFDNLSKVKRDMLLRKARKLNG
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA