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The map label for this gene is def [H]

Identifier: 110678658

GI number: 110678658

Start: 1287389

End: 1287916

Strand: Direct

Name: def [H]

Synonym: RD1_1337

Alternate gene names: 110678658

Gene position: 1287389-1287916 (Clockwise)

Preceding gene: 110678656

Following gene: 110678659

Centisome position: 31.15

GC content: 57.95

Gene sequence:

>528_bases
ATGAAAAACATGAAACGAAGTATCATTCTGCACCCTGATCCGCGGCTCAAGAAAACGGCAGAGCCTGTTGCCGACATCAG
TGATGAGTTGCGCAGGCTTGGCGATGACATGCTTGAGACGATGTATGATGCGCCGGGCATCGGGCTTGCGGCACCGCAGG
TGGGCATTCTGCAGCGGGTTGTCGTGATGGATTGTATCAAGGAGCCGGGCGATACGCCGCGCCCGGTGATCATGTTCAAC
CCCGAAGTCATCGCCACCTCTGACGAGACCAGTGTTTACGAGGAAGGGTGCCTGTCGATCCCCGAGCAATTCGCAGAGGT
GACCCGCCCGGCAGAAGTCGAAGTGCGCTGGATGGACCGGGATGGTGCGGCACAGTCTGAAGTGTTTACCGGGCTGTGGG
CCACATGCGCCCAGCATGAGATCGACCATTTGGATGGCAAGCTGTTTATTGACTACCTCAGGCCGCTGCGCCGCCAGATG
ATTACCCGCAAGATGGTCAAGCTGAAACGGGAACTGGCCCGCACATGA

Upstream 100 bases:

>100_bases
TGACATGATGTGCTCCTGTGTGTGCTTCGGGCGCAAGCTAGCGGGAAACGAGGCGAGCGCAAGTCCGAGATCAGTTGCAC
AGCACGGGCGCTTGACCTAA

Downstream 100 bases:

>100_bases
GCGTACGCGCTTACCTTAAATGGCCAGACGCGCGGCTGCGCACTGCCGCCGCCGATGTAGCGGAGATCACGCCGGAGGTG
CTGGGCATCTGGGATGATAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKNMKRSIILHPDPRLKKTAEPVADISDELRRLGDDMLETMYDAPGIGLAAPQVGILQRVVVMDCIKEPGDTPRPVIMFN
PEVIATSDETSVYEEGCLSIPEQFAEVTRPAEVEVRWMDRDGAAQSEVFTGLWATCAQHEIDHLDGKLFIDYLRPLRRQM
ITRKMVKLKRELART

Sequences:

>Translated_175_residues
MKNMKRSIILHPDPRLKKTAEPVADISDELRRLGDDMLETMYDAPGIGLAAPQVGILQRVVVMDCIKEPGDTPRPVIMFN
PEVIATSDETSVYEEGCLSIPEQFAEVTRPAEVEVRWMDRDGAAQSEVFTGLWATCAQHEIDHLDGKLFIDYLRPLRRQM
ITRKMVKLKRELART
>Mature_175_residues
MKNMKRSIILHPDPRLKKTAEPVADISDELRRLGDDMLETMYDAPGIGLAAPQVGILQRVVVMDCIKEPGDTPRPVIMFN
PEVIATSDETSVYEEGCLSIPEQFAEVTRPAEVEVRWMDRDGAAQSEVFTGLWATCAQHEIDHLDGKLFIDYLRPLRRQM
ITRKMVKLKRELART

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=51.1904761904762, Blast_Score=155, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24645728, Length=155, Percent_Identity=32.258064516129, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=28.4810126582279, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19892; Mature: 19892

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMKRSIILHPDPRLKKTAEPVADISDELRRLGDDMLETMYDAPGIGLAAPQVGILQRV
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VVMDCIKEPGDTPRPVIMFNPEVIATSDETSVYEEGCLSIPEQFAEVTRPAEVEVRWMDR
HHHHHHHCCCCCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DGAAQSEVFTGLWATCAQHEIDHLDGKLFIDYLRPLRRQMITRKMVKLKRELART
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNMKRSIILHPDPRLKKTAEPVADISDELRRLGDDMLETMYDAPGIGLAAPQVGILQRV
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VVMDCIKEPGDTPRPVIMFNPEVIATSDETSVYEEGCLSIPEQFAEVTRPAEVEVRWMDR
HHHHHHHCCCCCCCCEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DGAAQSEVFTGLWATCAQHEIDHLDGKLFIDYLRPLRRQMITRKMVKLKRELART
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]