| Definition | Roseobacter denitrificans OCh 114, complete genome. |
|---|---|
| Accession | NC_008209 |
| Length | 4,133,097 |
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The map label for this gene is nas
Identifier: 110678627
GI number: 110678627
Start: 1262010
End: 1263047
Strand: Reverse
Name: nas
Synonym: RD1_1303
Alternate gene names: NA
Gene position: 1263047-1262010 (Counterclockwise)
Preceding gene: 110678628
Following gene: 110678625
Centisome position: 30.56
GC content: 58.48
Gene sequence:
>1038_bases ATGTCCGAACATGATGAGGAAGATTGCTGCTGCGGAGGTCTGTTAACCAGTAACGACGTCCGAACCGGCATCATCTCCGG CGATACTTTCAAGAACAAGACGGTTCAATACGCGGCAGTCGATGGGCTTGCCGTGTTTGAAGGCTGCATCGTGCTTGGCA CGGTGGAGGAAGTCGAAGAGCGCACTGCGGCGGCCAAGGCTGCCCTTGATGCAGGCGATGATCCAGACGAGATCGCACAT GGCGTGGTGATCACGGGGGCCAATCGGCGCTGGCCGGGCGCGTTGATGCCATATGAAATCGACCCGGCCCTGCCCGCAAC CAACGTGCAACGCGTCAATGATGCCATCGCCCACTGGAAACAGAAAACCGGCATGCGCTTTGTGCTGCGCACGAATGCGA ACAAGGCGCAATATCCCAATTACGTGCGGTTTCGCCCTGCCACGGGCTGTTGGAGTCAGGTCGGCATGCGCGGCGGCAAG CAGGAAATCGGCCTTGCCGGTGGCTGCGGGTTTGGCGCGACCGTCCACGAGATCGGCCATGCGTTCGGGCTCTGGCACGA ACAAAGCCGGGAAGACCGCGATACCAAGGTCAAGATCAACTGGCAGAACATCACCGCGGGCAAAGAGCATAACTTCAACC AGCACATTGCGGATGGGGATGACGTGGGACCCTATGACTACGGGTCCATCATGCACTATGGCCGCTATGCGTTCTCCAAG AACGGCCAGCCGACCATTGAGTCGATCCCACCGGGCAAGACGCTGGGTCAGCGCACCGGGCTGAGTGCCGGTGATATTGT CGCCATCCAGAGCATCTATCAGCTATGGGAAACCGTCGATGTTGCACGGGTCTATGCGACCTCGGGCACAAAGAACTGCT GGGTCATGCCGAAAGGTCAAGGGTGGTTCCGCATTGATCCGGACACCACCGATGGATGCAGCAATGTGTTCGACATCTGC TGTTCGGCGATGGCCTTCGGCAAGAAGCTGTCCCTTTTCAAGGACGGCGACAAGGTCTACCGCGCTCAACTATTATAA
Upstream 100 bases:
>100_bases TGCCAGAGTGGCGGTATTTTGCCAACCTTTGAGGGCCGCATCAGATTTAGCGGCGACAATTTTTTGTGTTTAACAAAGTC AATTTTAAAGGAATATCAGC
Downstream 100 bases:
>100_bases CTTCTTTCCAGTATCATTTTTATCAGGAGAATTTCTCATGTGGCATAACAACAAAACCGTTTCACGCACCTATACCTCTA AAGACTCGCAGAACTGTCAT
Product: zinc metalloproteinase nas-13 precursor, putative
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 345; Mature: 344
Protein sequence:
>345_residues MSEHDEEDCCCGGLLTSNDVRTGIISGDTFKNKTVQYAAVDGLAVFEGCIVLGTVEEVEERTAAAKAALDAGDDPDEIAH GVVITGANRRWPGALMPYEIDPALPATNVQRVNDAIAHWKQKTGMRFVLRTNANKAQYPNYVRFRPATGCWSQVGMRGGK QEIGLAGGCGFGATVHEIGHAFGLWHEQSREDRDTKVKINWQNITAGKEHNFNQHIADGDDVGPYDYGSIMHYGRYAFSK NGQPTIESIPPGKTLGQRTGLSAGDIVAIQSIYQLWETVDVARVYATSGTKNCWVMPKGQGWFRIDPDTTDGCSNVFDIC CSAMAFGKKLSLFKDGDKVYRAQLL
Sequences:
>Translated_345_residues MSEHDEEDCCCGGLLTSNDVRTGIISGDTFKNKTVQYAAVDGLAVFEGCIVLGTVEEVEERTAAAKAALDAGDDPDEIAH GVVITGANRRWPGALMPYEIDPALPATNVQRVNDAIAHWKQKTGMRFVLRTNANKAQYPNYVRFRPATGCWSQVGMRGGK QEIGLAGGCGFGATVHEIGHAFGLWHEQSREDRDTKVKINWQNITAGKEHNFNQHIADGDDVGPYDYGSIMHYGRYAFSK NGQPTIESIPPGKTLGQRTGLSAGDIVAIQSIYQLWETVDVARVYATSGTKNCWVMPKGQGWFRIDPDTTDGCSNVFDIC CSAMAFGKKLSLFKDGDKVYRAQLL >Mature_344_residues SEHDEEDCCCGGLLTSNDVRTGIISGDTFKNKTVQYAAVDGLAVFEGCIVLGTVEEVEERTAAAKAALDAGDDPDEIAHG VVITGANRRWPGALMPYEIDPALPATNVQRVNDAIAHWKQKTGMRFVLRTNANKAQYPNYVRFRPATGCWSQVGMRGGKQ EIGLAGGCGFGATVHEIGHAFGLWHEQSREDRDTKVKINWQNITAGKEHNFNQHIADGDDVGPYDYGSIMHYGRYAFSKN GQPTIESIPPGKTLGQRTGLSAGDIVAIQSIYQLWETVDVARVYATSGTKNCWVMPKGQGWFRIDPDTTDGCSNVFDICC SAMAFGKKLSLFKDGDKVYRAQLL
Specific function: Zinc metallendopeptidase that cleaves preferentially on amino-terminal side of aspartate-containing substrates [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ricin B-type lectin domain [H]
Homologues:
Organism=Homo sapiens, GI153070264, Length=197, Percent_Identity=38.5786802030457, Blast_Score=129, Evalue=4e-30, Organism=Homo sapiens, GI157502169, Length=200, Percent_Identity=35, Blast_Score=127, Evalue=2e-29, Organism=Homo sapiens, GI22547221, Length=199, Percent_Identity=37.1859296482412, Blast_Score=119, Evalue=5e-27, Organism=Homo sapiens, GI5453579, Length=197, Percent_Identity=37.0558375634518, Blast_Score=114, Evalue=1e-25, Organism=Homo sapiens, GI6912724, Length=198, Percent_Identity=36.3636363636364, Blast_Score=114, Evalue=1e-25, Organism=Homo sapiens, GI4502421, Length=197, Percent_Identity=37.0558375634518, Blast_Score=114, Evalue=2e-25, Organism=Homo sapiens, GI153070262, Length=142, Percent_Identity=41.5492957746479, Blast_Score=107, Evalue=2e-23, Organism=Caenorhabditis elegans, GI71989620, Length=187, Percent_Identity=41.1764705882353, Blast_Score=144, Evalue=5e-35, Organism=Caenorhabditis elegans, GI115532764, Length=213, Percent_Identity=40.3755868544601, Blast_Score=141, Evalue=5e-34, Organism=Caenorhabditis elegans, GI71981168, Length=191, Percent_Identity=37.696335078534, Blast_Score=133, Evalue=2e-31, Organism=Caenorhabditis elegans, GI71995692, Length=191, Percent_Identity=39.7905759162304, Blast_Score=132, Evalue=3e-31, Organism=Caenorhabditis elegans, GI71986189, Length=245, Percent_Identity=37.1428571428571, Blast_Score=127, Evalue=8e-30, Organism=Caenorhabditis elegans, GI71981193, Length=193, Percent_Identity=35.2331606217617, Blast_Score=117, Evalue=7e-27, Organism=Caenorhabditis elegans, GI25152806, Length=200, Percent_Identity=35.5, Blast_Score=115, Evalue=4e-26, Organism=Caenorhabditis elegans, GI193210526, Length=169, Percent_Identity=36.6863905325444, Blast_Score=111, Evalue=5e-25, Organism=Caenorhabditis elegans, GI71983097, Length=225, Percent_Identity=30.6666666666667, Blast_Score=110, Evalue=1e-24, Organism=Caenorhabditis elegans, GI86562912, Length=184, Percent_Identity=34.7826086956522, Blast_Score=108, Evalue=5e-24, Organism=Caenorhabditis elegans, GI17568533, Length=192, Percent_Identity=36.9791666666667, Blast_Score=107, Evalue=6e-24, Organism=Caenorhabditis elegans, GI25149641, Length=262, Percent_Identity=30.5343511450382, Blast_Score=107, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71996833, Length=235, Percent_Identity=34.0425531914894, Blast_Score=107, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71983649, Length=237, Percent_Identity=34.5991561181435, Blast_Score=107, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71989946, Length=240, Percent_Identity=36.25, Blast_Score=107, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71980967, Length=234, Percent_Identity=31.1965811965812, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71989942, Length=202, Percent_Identity=38.1188118811881, Blast_Score=106, Evalue=1e-23, Organism=Caenorhabditis elegans, GI71983640, Length=237, Percent_Identity=34.5991561181435, Blast_Score=106, Evalue=2e-23, Organism=Caenorhabditis elegans, GI71986142, Length=199, Percent_Identity=30.1507537688442, Blast_Score=103, Evalue=1e-22, Organism=Caenorhabditis elegans, GI72000269, Length=163, Percent_Identity=36.1963190184049, Blast_Score=101, Evalue=6e-22, Organism=Caenorhabditis elegans, GI25150905, Length=186, Percent_Identity=36.5591397849462, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI71996558, Length=166, Percent_Identity=34.3373493975904, Blast_Score=94, Evalue=9e-20, Organism=Caenorhabditis elegans, GI71996566, Length=166, Percent_Identity=34.3373493975904, Blast_Score=94, Evalue=1e-19, Organism=Caenorhabditis elegans, GI71996019, Length=208, Percent_Identity=29.8076923076923, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17562122, Length=192, Percent_Identity=30.7291666666667, Blast_Score=87, Evalue=2e-17, Organism=Caenorhabditis elegans, GI115532766, Length=98, Percent_Identity=46.9387755102041, Blast_Score=85, Evalue=5e-17, Organism=Caenorhabditis elegans, GI71993705, Length=197, Percent_Identity=32.48730964467, Blast_Score=83, Evalue=2e-16, Organism=Caenorhabditis elegans, GI212645453, Length=193, Percent_Identity=33.6787564766839, Blast_Score=82, Evalue=6e-16, Organism=Caenorhabditis elegans, GI17568805, Length=210, Percent_Identity=30, Blast_Score=81, Evalue=6e-16, Organism=Caenorhabditis elegans, GI72000541, Length=134, Percent_Identity=35.8208955223881, Blast_Score=80, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17509235, Length=216, Percent_Identity=26.8518518518519, Blast_Score=79, Evalue=5e-15, Organism=Caenorhabditis elegans, GI17534131, Length=199, Percent_Identity=31.1557788944724, Blast_Score=78, Evalue=7e-15, Organism=Caenorhabditis elegans, GI17562124, Length=99, Percent_Identity=41.4141414141414, Blast_Score=77, Evalue=2e-14, Organism=Caenorhabditis elegans, GI32566667, Length=189, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-14, Organism=Caenorhabditis elegans, GI25149215, Length=189, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=5e-14, Organism=Caenorhabditis elegans, GI71993835, Length=197, Percent_Identity=28.9340101522843, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI71991562, Length=163, Percent_Identity=31.2883435582822, Blast_Score=71, Evalue=1e-12, Organism=Drosophila melanogaster, GI21355491, Length=191, Percent_Identity=39.7905759162304, Blast_Score=134, Evalue=1e-31, Organism=Drosophila melanogaster, GI24649626, Length=201, Percent_Identity=35.8208955223881, Blast_Score=129, Evalue=3e-30, Organism=Drosophila melanogaster, GI24643089, Length=194, Percent_Identity=39.6907216494845, Blast_Score=128, Evalue=6e-30, Organism=Drosophila melanogaster, GI17136740, Length=220, Percent_Identity=36.8181818181818, Blast_Score=121, Evalue=5e-28, Organism=Drosophila melanogaster, GI24649765, Length=220, Percent_Identity=36.8181818181818, Blast_Score=121, Evalue=5e-28, Organism=Drosophila melanogaster, GI45549230, Length=204, Percent_Identity=37.2549019607843, Blast_Score=119, Evalue=2e-27, Organism=Drosophila melanogaster, GI24644493, Length=201, Percent_Identity=35.3233830845771, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI19921390, Length=197, Percent_Identity=35.0253807106599, Blast_Score=115, Evalue=6e-26, Organism=Drosophila melanogaster, GI19921388, Length=196, Percent_Identity=36.2244897959184, Blast_Score=114, Evalue=1e-25, Organism=Drosophila melanogaster, GI20129545, Length=196, Percent_Identity=36.734693877551, Blast_Score=112, Evalue=3e-25, Organism=Drosophila melanogaster, GI19921382, Length=200, Percent_Identity=33, Blast_Score=107, Evalue=1e-23, Organism=Drosophila melanogaster, GI19921386, Length=195, Percent_Identity=35.3846153846154, Blast_Score=106, Evalue=2e-23, Organism=Drosophila melanogaster, GI24646966, Length=202, Percent_Identity=33.1683168316832, Blast_Score=89, Evalue=4e-18, Organism=Drosophila melanogaster, GI19921384, Length=197, Percent_Identity=31.4720812182741, Blast_Score=81, Evalue=9e-16,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001506 - InterPro: IPR006026 - InterPro: IPR008997 - InterPro: IPR000772 [H]
Pfam domain/function: PF01400 Astacin; PF00652 Ricin_B_lectin [H]
EC number: =3.4.24.76 [H]
Molecular weight: Translated: 37693; Mature: 37561
Theoretical pI: Translated: 6.04; Mature: 6.04
Prosite motif: PS00142 ZINC_PROTEASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.9 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 4.9 %Cys+Met (Translated Protein) 2.9 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSEHDEEDCCCGGLLTSNDVRTGIISGDTFKNKTVQYAAVDGLAVFEGCIVLGTVEEVEE CCCCCCCCHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHCCEEEEECHHHHHH RTAAAKAALDAGDDPDEIAHGVVITGANRRWPGALMPYEIDPALPATNVQRVNDAIAHWK HHHHHHHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH QKTGMRFVLRTNANKAQYPNYVRFRPATGCWSQVGMRGGKQEIGLAGGCGFGATVHEIGH HHCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCHHCCEECCCCCCHHHHHHHH AFGLWHEQSREDRDTKVKINWQNITAGKEHNFNQHIADGDDVGPYDYGSIMHYGRYAFSK HHHCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC NGQPTIESIPPGKTLGQRTGLSAGDIVAIQSIYQLWETVDVARVYATSGTKNCWVMPKGQ CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCC GWFRIDPDTTDGCSNVFDICCSAMAFGKKLSLFKDGDKVYRAQLL CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHEECC >Mature Secondary Structure SEHDEEDCCCGGLLTSNDVRTGIISGDTFKNKTVQYAAVDGLAVFEGCIVLGTVEEVEE CCCCCCCHHCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCHHHHCCEEEEECHHHHHH RTAAAKAALDAGDDPDEIAHGVVITGANRRWPGALMPYEIDPALPATNVQRVNDAIAHWK HHHHHHHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHH QKTGMRFVLRTNANKAQYPNYVRFRPATGCWSQVGMRGGKQEIGLAGGCGFGATVHEIGH HHCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCCCHHCCEECCCCCCHHHHHHHH AFGLWHEQSREDRDTKVKINWQNITAGKEHNFNQHIADGDDVGPYDYGSIMHYGRYAFSK HHHCCHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC NGQPTIESIPPGKTLGQRTGLSAGDIVAIQSIYQLWETVDVARVYATSGTKNCWVMPKGQ CCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCC GWFRIDPDTTDGCSNVFDICCSAMAFGKKLSLFKDGDKVYRAQLL CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 7771796; 7768917 [H]