Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 110678603

GI number: 110678603

Start: 1240664

End: 1242121

Strand: Reverse

Name: dacA [H]

Synonym: RD1_1278

Alternate gene names: 110678603

Gene position: 1242121-1240664 (Counterclockwise)

Preceding gene: 110678608

Following gene: 110678594

Centisome position: 30.05

GC content: 58.98

Gene sequence:

>1458_bases
ATGAAGGTTCGGCGCACCTGGCCGGCCCGGTATGGGCTATATGCATTCGCGGTACTGTGGATGCTCGTTATCTTACCTCT
CACCGCAGCAGCCGCCCCCTATGCGGCGTTTGTGATGGATGCGCGGACGGGCGAGGTTTTACATTCCCGCAACGCAGATA
CGCGTTTGCACCCTGCATCCTTGACCAAGATGATGACGCTCTACATCGCTTTTCAAGCTGTTGAAAACGGGGAGATTTCG
CTTGATACCAAGGTCAAGATTTCCCGCTTTGCCGCTGCGGAACCTCCTTCAAAACTGGGGCTGAAATCAGGCCAGCGCAT
CGCCTTTCGCTACCTGATACGGGCCGCTGCGATCAAATCCGCCAATGATGCGGCGACCGCCATCGGTGAAGCACTCGAAG
GCTCAGAGGCGGCCTTTGCGCGGCGCATGAACCGAACCGCGAAAGAACTGGGGATGACCCGCACAACGTTCAAGAACGCG
CATGGTCTGACGGAAAGTGGGCATATGTCCACCGCGCGCGATATGTCGCTATTGGGGCGTCATGTGCTTTATGATTACCC
CGAATATTACAATCTGTTTTCTCGTCGGTCGACCCATGCCGGGGTCAGGGATGTTGCAAACACGAACCGCCGCCTTCTGG
CAGCCTACCGCGGAGCGGATGGTATCAAAACAGGATACACGCGTGCGGCGGGCTTCAATCTCGTTGCCTCCGCCGAGCGT
GGTCAGGAACGCATTATTGCAACCGTTTTCGGCGGCACCTCGACAATTGCGCGCAACAAGCGGGTTGCGGAGCTGCTCGA
CATGGGCTTCAAACGCGCGCCAAGCAGGGCAACCGTGCGCAAGCCCAAAAAGCCGTCCTATTTTGCGACACGACATCAGG
GGACACGGGACGCGACGATCATGACGGCCAGTTTGCGCCCACAGATCCGGCCGCTTGTCGCTGCGGTGGAAAGCCCGGTC
AATGCGGAGGACATAATCGCCGCAGTTGAGCAGGCAAAGACGTCGACAGCAAGCATCGCAAGCACCACGGGAATACTGAA
CAGCGATATCAAATCGGCCCTGATCGAAGCACAGGAAACCCCGTCAACAGGTTCTGCGGTCCCGATCGGCGGGGTCGCAC
CCGTGTCGATCAGGCCTGCCATGCGCCCCACGGACCTGATCAACGCCTCGCTGGACCAGTCAGCGGAAACCGAAGTCGTC
ACACGACTGTCGACATCAGGCGGGCGGCAATGGGGTGTGAATGTCGGGCAGTTTGGCAGCCGATATCTTGCCGAACGCGC
GTTGTTGACGACCGCGCTCACAGAGATGGAGACGCTGGATGGCACATTGCGCAAGGTTGTGCGGCGGTCGAGTGGGTTTG
ACGCCAACTTCCTCGGTATGACCGAAGACTCCGCAGATCTTGCATGCCGTCGCCTTCAGGCGCGCAACATCACATGCAAA
ACGGTGGGGCCATCCTGA

Upstream 100 bases:

>100_bases
AATTCAATGGTTTATTGAAACCAAAAGCTGTGTTAGCGTCAGCGCCAATAAAGTCAGCCGAAAAAATTCGGCGACATGAG
GCAGAAAAAAGGCGGTTATC

Downstream 100 bases:

>100_bases
GCCCTTGGCAAACCGCTTGTCACCTCCCGGCGCAAAACTGCGGCGGGCAACCACGGGTTATGATCGGCACAAAACCCTTC
GTGACGATTTTAAAAAGCTA

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKVRRTWPARYGLYAFAVLWMLVILPLTAAAAPYAAFVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFQAVENGEIS
LDTKVKISRFAAAEPPSKLGLKSGQRIAFRYLIRAAAIKSANDAATAIGEALEGSEAAFARRMNRTAKELGMTRTTFKNA
HGLTESGHMSTARDMSLLGRHVLYDYPEYYNLFSRRSTHAGVRDVANTNRRLLAAYRGADGIKTGYTRAAGFNLVASAER
GQERIIATVFGGTSTIARNKRVAELLDMGFKRAPSRATVRKPKKPSYFATRHQGTRDATIMTASLRPQIRPLVAAVESPV
NAEDIIAAVEQAKTSTASIASTTGILNSDIKSALIEAQETPSTGSAVPIGGVAPVSIRPAMRPTDLINASLDQSAETEVV
TRLSTSGGRQWGVNVGQFGSRYLAERALLTTALTEMETLDGTLRKVVRRSSGFDANFLGMTEDSADLACRRLQARNITCK
TVGPS

Sequences:

>Translated_485_residues
MKVRRTWPARYGLYAFAVLWMLVILPLTAAAAPYAAFVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFQAVENGEIS
LDTKVKISRFAAAEPPSKLGLKSGQRIAFRYLIRAAAIKSANDAATAIGEALEGSEAAFARRMNRTAKELGMTRTTFKNA
HGLTESGHMSTARDMSLLGRHVLYDYPEYYNLFSRRSTHAGVRDVANTNRRLLAAYRGADGIKTGYTRAAGFNLVASAER
GQERIIATVFGGTSTIARNKRVAELLDMGFKRAPSRATVRKPKKPSYFATRHQGTRDATIMTASLRPQIRPLVAAVESPV
NAEDIIAAVEQAKTSTASIASTTGILNSDIKSALIEAQETPSTGSAVPIGGVAPVSIRPAMRPTDLINASLDQSAETEVV
TRLSTSGGRQWGVNVGQFGSRYLAERALLTTALTEMETLDGTLRKVVRRSSGFDANFLGMTEDSADLACRRLQARNITCK
TVGPS
>Mature_485_residues
MKVRRTWPARYGLYAFAVLWMLVILPLTAAAAPYAAFVMDARTGEVLHSRNADTRLHPASLTKMMTLYIAFQAVENGEIS
LDTKVKISRFAAAEPPSKLGLKSGQRIAFRYLIRAAAIKSANDAATAIGEALEGSEAAFARRMNRTAKELGMTRTTFKNA
HGLTESGHMSTARDMSLLGRHVLYDYPEYYNLFSRRSTHAGVRDVANTNRRLLAAYRGADGIKTGYTRAAGFNLVASAER
GQERIIATVFGGTSTIARNKRVAELLDMGFKRAPSRATVRKPKKPSYFATRHQGTRDATIMTASLRPQIRPLVAAVESPV
NAEDIIAAVEQAKTSTASIASTTGILNSDIKSALIEAQETPSTGSAVPIGGVAPVSIRPAMRPTDLINASLDQSAETEVV
TRLSTSGGRQWGVNVGQFGSRYLAERALLTTALTEMETLDGTLRKVVRRSSGFDANFLGMTEDSADLACRRLQARNITCK
TVGPS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=244, Percent_Identity=39.7540983606557, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1786851, Length=241, Percent_Identity=39.0041493775934, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI87082022, Length=254, Percent_Identity=35.8267716535433, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082054, Length=259, Percent_Identity=27.4131274131274, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52481; Mature: 52481

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRRTWPARYGLYAFAVLWMLVILPLTAAAAPYAAFVMDARTGEVLHSRNADTRLHPAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHCCCCCCCCCHHH
LTKMMTLYIAFQAVENGEISLDTKVKISRFAAAEPPSKLGLKSGQRIAFRYLIRAAAIKS
HHHHHHHHHHHHHHCCCCEEECCCEEEHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCC
ANDAATAIGEALEGSEAAFARRMNRTAKELGMTRTTFKNAHGLTESGHMSTARDMSLLGR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
HVLYDYPEYYNLFSRRSTHAGVRDVANTNRRLLAAYRGADGIKTGYTRAAGFNLVASAER
HHHHCCHHHHHHHHHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCEEECCCC
GQERIIATVFGGTSTIARNKRVAELLDMGFKRAPSRATVRKPKKPSYFATRHQGTRDATI
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEE
MTASLRPQIRPLVAAVESPVNAEDIIAAVEQAKTSTASIASTTGILNSDIKSALIEAQET
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
PSTGSAVPIGGVAPVSIRPAMRPTDLINASLDQSAETEVVTRLSTSGGRQWGVNVGQFGS
CCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCHHHHHH
RYLAERALLTTALTEMETLDGTLRKVVRRSSGFDANFLGMTEDSADLACRRLQARNITCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHCCCEEE
TVGPS
ECCCC
>Mature Secondary Structure
MKVRRTWPARYGLYAFAVLWMLVILPLTAAAAPYAAFVMDARTGEVLHSRNADTRLHPAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCHHHHCCCCCCCCCHHH
LTKMMTLYIAFQAVENGEISLDTKVKISRFAAAEPPSKLGLKSGQRIAFRYLIRAAAIKS
HHHHHHHHHHHHHHCCCCEEECCCEEEHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCC
ANDAATAIGEALEGSEAAFARRMNRTAKELGMTRTTFKNAHGLTESGHMSTARDMSLLGR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHH
HVLYDYPEYYNLFSRRSTHAGVRDVANTNRRLLAAYRGADGIKTGYTRAAGFNLVASAER
HHHHCCHHHHHHHHHCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHCCCCEEECCCC
GQERIIATVFGGTSTIARNKRVAELLDMGFKRAPSRATVRKPKKPSYFATRHQGTRDATI
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCEE
MTASLRPQIRPLVAAVESPVNAEDIIAAVEQAKTSTASIASTTGILNSDIKSALIEAQET
EEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
PSTGSAVPIGGVAPVSIRPAMRPTDLINASLDQSAETEVVTRLSTSGGRQWGVNVGQFGS
CCCCCCCCCCCCCCCEECCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCEECCCHHHHHH
RYLAERALLTTALTEMETLDGTLRKVVRRSSGFDANFLGMTEDSADLACRRLQARNITCK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHCCCEEE
TVGPS
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]