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The map label for this gene is leuA [H]

Identifier: 110678539

GI number: 110678539

Start: 1174978

End: 1176546

Strand: Reverse

Name: leuA [H]

Synonym: RD1_1211

Alternate gene names: 110678539

Gene position: 1176546-1174978 (Counterclockwise)

Preceding gene: 110678542

Following gene: 110678538

Centisome position: 28.47

GC content: 59.27

Gene sequence:

>1569_bases
ATGACAGATACGACGAAACAGGATCACGTATTGATCTTTGACACGACATTGCGCGACGGCGAACAAAGCCCGGGTGCGAC
CATGACCCATTCGGAAAAGCTTGAGATCGCCGGGCTGCTGGATGAAATGGGTGTCGATATCATCGAAGCAGGCTTTCCCA
TTGCGTCAGAGGGCGATTTCGCCGCTGTCTCTGAAATAGCGCGCAACGCCAAGACATCGGTGATTTGTGGTCTGGCGCGC
GCCCAGATCGGGGACATCGACAGATGTTGGGAGGCCGTGAAACACGCCAAACGCCCGCGCATCCACACCTTTATCGGGAC
GTCCCCCCTGCACCGCGCCATTCCGAACCTCGATATGGACCAGATGGCCGAACGGATCGAACAGACCGTGACCCACGCGC
GCAACCTGTGCGACAATGTGCAGTGGTCGCCCATGGATGCCACCCGCACCGAAGAGGATTACCTCTGCCGGGTGGTGGAA
ATTGCCATCAAATGCGGGGCCACCACGATCAATATCCCCGACACCGTCGGCTATACGGCGCCGCGCGAATCCGCAGCGCT
CATCTCGATGCTGCTTGAACGAGTGCCGGGCGCAGATGAGATCACCTTTGCCACCCACTGCCACAATGATCTGGGCATGG
CCACGGCCAACAGCCTTGCCGCCGTCGAAGCGGGCGCGCGCCAGATCGAATGCACGATCAACGGCCTTGGCGAACGTGCA
GGGAATACAGCGCTGGAAGAGGTCGTGATGGCCCTGAAAGTGCGCAATGACATCATGCCGTTTCACACCGGCATCGACGC
CACCAAGATCATGAATATCTCGCGCCGTGTCGCTGCTGTGTCCGGCTTCCCGGTGCAGTTCAACAAGGCGATCGTCGGCA
AGAACGCCTTTGCGCATGAATCCGGCATCCATCAGGACGGCATGCTCAAGAACGCGGAAACCTTTGAGATCATGCGCCCC
GAGGATGTTGGCCTGACCGAAACCAATATCGTGATGGGCAAACATTCAGGGCGCGCAGCGTTGCGCTCCAAGCTGGAGAA
CCTCGGTTTTGAATTGGGCGACAACCAGCTCAAGGACGTCTTTGTGCGTTTCAAGGCGTTGGCGGACCGCAAGAAAGAGA
TCTATGACGACGATCTGATCGCCTTGATGCGCACGGCGACGGACCCTGAAAACGACCGGATCAAACTGGTGTCGCTGCGC
GTCGTCTGCGGCACCCACGGCCCTCAGCAAGCTGACCTTGTGCTGAACATCGACGGTGTAGAGAAATCCACCACACAAAC
CGGGGACGGCCCGGTTGATGCGTCCTTCAATGCAATCAAGGCACTGGTTTCGCATTCGGCACGCCTTCAGCTTTATCAGG
TGCATGCAGTGACCGAAGGCACGGATGCGCAGGCAACCGTTTCGGTGCGGATGGAGGAAGAGGGCCGTATCGTCACGGGG
CAGTCGGCAGACACGGACACGGTGGTAGCCTCGGCGCGCGCCTATATCCACGCCTTGAACCGCCTGCTGGTGCGGCGCGA
AAAGGCAGGCAAGGACGCGCGCGAAATCAGCTATAAGGACGTGGGCTGA

Upstream 100 bases:

>100_bases
CCTACTGCTGCTAATCCGCCTCTGAGCGTGCCCTTCGGCGCGCCCGCTCAGAGGGATGTGTTGCGCGCCACGGATTGAAG
CACAGAACGAAAGATACACC

Downstream 100 bases:

>100_bases
CACCTTGGGCGGAATAGCGCGCGAGGTGCGATAAAATACAGATACCGCCGATTTTCCGGCGGTAACTTCTATTTTTATCT
GCTAGGCTTTATTGAACGCG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MTDTTKQDHVLIFDTTLRDGEQSPGATMTHSEKLEIAGLLDEMGVDIIEAGFPIASEGDFAAVSEIARNAKTSVICGLAR
AQIGDIDRCWEAVKHAKRPRIHTFIGTSPLHRAIPNLDMDQMAERIEQTVTHARNLCDNVQWSPMDATRTEEDYLCRVVE
IAIKCGATTINIPDTVGYTAPRESAALISMLLERVPGADEITFATHCHNDLGMATANSLAAVEAGARQIECTINGLGERA
GNTALEEVVMALKVRNDIMPFHTGIDATKIMNISRRVAAVSGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAETFEIMRP
EDVGLTETNIVMGKHSGRAALRSKLENLGFELGDNQLKDVFVRFKALADRKKEIYDDDLIALMRTATDPENDRIKLVSLR
VVCGTHGPQQADLVLNIDGVEKSTTQTGDGPVDASFNAIKALVSHSARLQLYQVHAVTEGTDAQATVSVRMEEEGRIVTG
QSADTDTVVASARAYIHALNRLLVRREKAGKDAREISYKDVG

Sequences:

>Translated_522_residues
MTDTTKQDHVLIFDTTLRDGEQSPGATMTHSEKLEIAGLLDEMGVDIIEAGFPIASEGDFAAVSEIARNAKTSVICGLAR
AQIGDIDRCWEAVKHAKRPRIHTFIGTSPLHRAIPNLDMDQMAERIEQTVTHARNLCDNVQWSPMDATRTEEDYLCRVVE
IAIKCGATTINIPDTVGYTAPRESAALISMLLERVPGADEITFATHCHNDLGMATANSLAAVEAGARQIECTINGLGERA
GNTALEEVVMALKVRNDIMPFHTGIDATKIMNISRRVAAVSGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAETFEIMRP
EDVGLTETNIVMGKHSGRAALRSKLENLGFELGDNQLKDVFVRFKALADRKKEIYDDDLIALMRTATDPENDRIKLVSLR
VVCGTHGPQQADLVLNIDGVEKSTTQTGDGPVDASFNAIKALVSHSARLQLYQVHAVTEGTDAQATVSVRMEEEGRIVTG
QSADTDTVVASARAYIHALNRLLVRREKAGKDAREISYKDVG
>Mature_521_residues
TDTTKQDHVLIFDTTLRDGEQSPGATMTHSEKLEIAGLLDEMGVDIIEAGFPIASEGDFAAVSEIARNAKTSVICGLARA
QIGDIDRCWEAVKHAKRPRIHTFIGTSPLHRAIPNLDMDQMAERIEQTVTHARNLCDNVQWSPMDATRTEEDYLCRVVEI
AIKCGATTINIPDTVGYTAPRESAALISMLLERVPGADEITFATHCHNDLGMATANSLAAVEAGARQIECTINGLGERAG
NTALEEVVMALKVRNDIMPFHTGIDATKIMNISRRVAAVSGFPVQFNKAIVGKNAFAHESGIHQDGMLKNAETFEIMRPE
DVGLTETNIVMGKHSGRAALRSKLENLGFELGDNQLKDVFVRFKALADRKKEIYDDDLIALMRTATDPENDRIKLVSLRV
VCGTHGPQQADLVLNIDGVEKSTTQTGDGPVDASFNAIKALVSHSARLQLYQVHAVTEGTDAQATVSVRMEEEGRIVTGQ
SADTDTVVASARAYIHALNRLLVRREKAGKDAREISYKDVG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=498, Percent_Identity=45.7831325301205, Blast_Score=420, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320071, Length=377, Percent_Identity=31.2997347480106, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=377, Percent_Identity=31.0344827586207, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324225, Length=545, Percent_Identity=24.7706422018349, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=554, Percent_Identity=24.9097472924188, Blast_Score=116, Evalue=9e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56852; Mature: 56721

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTKQDHVLIFDTTLRDGEQSPGATMTHSEKLEIAGLLDEMGVDIIEAGFPIASEGDF
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCH
AAVSEIARNAKTSVICGLARAQIGDIDRCWEAVKHAKRPRIHTFIGTSPLHRAIPNLDMD
HHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHH
QMAERIEQTVTHARNLCDNVQWSPMDATRTEEDYLCRVVEIAIKCGATTINIPDTVGYTA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PRESAALISMLLERVPGADEITFATHCHNDLGMATANSLAAVEAGARQIECTINGLGERA
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCHHHHHHCCCEEEEEEEECCCCCC
GNTALEEVVMALKVRNDIMPFHTGIDATKIMNISRRVAAVSGFPVQFNKAIVGKNAFAHE
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEECCCCHHHC
SGIHQDGMLKNAETFEIMRPEDVGLTETNIVMGKHSGRAALRSKLENLGFELGDNQLKDV
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHH
FVRFKALADRKKEIYDDDLIALMRTATDPENDRIKLVSLRVVCGTHGPQQADLVLNIDGV
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCC
EKSTTQTGDGPVDASFNAIKALVSHSARLQLYQVHAVTEGTDAQATVSVRMEEEGRIVTG
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEEEEECCCCEEEC
QSADTDTVVASARAYIHALNRLLVRREKAGKDAREISYKDVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure 
TDTTKQDHVLIFDTTLRDGEQSPGATMTHSEKLEIAGLLDEMGVDIIEAGFPIASEGDF
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCH
AAVSEIARNAKTSVICGLARAQIGDIDRCWEAVKHAKRPRIHTFIGTSPLHRAIPNLDMD
HHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHH
QMAERIEQTVTHARNLCDNVQWSPMDATRTEEDYLCRVVEIAIKCGATTINIPDTVGYTA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCC
PRESAALISMLLERVPGADEITFATHCHNDLGMATANSLAAVEAGARQIECTINGLGERA
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCHHHHHHCCCEEEEEEEECCCCCC
GNTALEEVVMALKVRNDIMPFHTGIDATKIMNISRRVAAVSGFPVQFNKAIVGKNAFAHE
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCEEECCCCHHHC
SGIHQDGMLKNAETFEIMRPEDVGLTETNIVMGKHSGRAALRSKLENLGFELGDNQLKDV
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHH
FVRFKALADRKKEIYDDDLIALMRTATDPENDRIKLVSLRVVCGTHGPQQADLVLNIDGV
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCC
EKSTTQTGDGPVDASFNAIKALVSHSARLQLYQVHAVTEGTDAQATVSVRMEEEGRIVTG
CCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEEEEEEECCCCEEEC
QSADTDTVVASARAYIHALNRLLVRREKAGKDAREISYKDVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA