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The map label for this gene is citE [H]

Identifier: 110678345

GI number: 110678345

Start: 963676

End: 964632

Strand: Reverse

Name: citE [H]

Synonym: RD1_1001

Alternate gene names: 110678345

Gene position: 964632-963676 (Counterclockwise)

Preceding gene: 110678350

Following gene: 110678343

Centisome position: 23.34

GC content: 62.28

Gene sequence:

>957_bases
ATGAGCTTTCGCCTTCAGCCCACTGCCCCTGCACGCCCGAACCGCTGTCAGCTTTTTGGCCCCGGGTCACGTCCCGCCAT
TTTCGAGAAAATGGCAACTTCCGCGGCGGATGTCATCAATCTTGACCTCGAAGACAGTGTCGCGCCCAGCGACAAGGACA
CCGCGCGTGCCAATATCATTCAGGCGATCTCCGACATCGACTGGGGCACCAAGACGCTTTCGGTCCGCATCAACGGGCTG
GACACGCCCTATTGGTACAAGGACGTGGTGGACCTGCTGGAACAGGCGGGCGAGCGGCTGGATCAGATCATGATCCCCAA
GGTCGGCTGCGCGGGCGACATTTATGCCGTGGATGCGCTGGTCACAGCGATTGAGGCCGCCAAGGGCCGCAACAAGAAGA
TCACCTTTGAGGTCATCATCGAATCTGCGGCCGGCATCGCCCATGTGGAAGAAATCGCCTCCGCCTCCCCCCGCATGGCC
GCGATGAGCCTCGGGGCTGCCGATTTTGCCGCCTCCATGGGCATGCAAACCACAGGCATCGGCGGCACGCAGGAGAATTA
CTACATGCTGCGCGAGGGCCAGAAATACTGGTCAGACCCCTGGCATTGGGCGCAAACGGCCATCGTGGCCGCCTGCCGGA
CGCATGGCGTTCTGCCGGTGGATGGCCCCTTCGGCGATTTCTCGGACGACGAAGGGTTCCGGGCGCAGGCACGGCGGTCT
GCCACGCTGGGCATGGTGGGCAAATGGGCGATCCACCCCAAGCAGATCGCCATCGCCAATGAGGTCTTTACCCCCTCCGA
GGACGCTGTGCGCGAGGCGCGTGAAATTCTGGCGGCCATGGAACAGGCCAAAGCCAATGGCGAAGGGGCGACCGTTTACA
AGGGGCGGCTGGTTGATATCGCCTCGATCAAACAGGCCGAAGTGATCGTGCGCCAGTCGGAGATGATCGCCTCCTGA

Upstream 100 bases:

>100_bases
TTTGTTTTGCTGCAACTGCGAAATGCACTTGCAGCATCTTGCGCAATTTAATATCAGAACCGCGATAGTATTTGACATAA
AATTACCCATGAGGTCCCAA

Downstream 100 bases:

>100_bases
GCGGGGCGATCACCCTCAGGCGCGTTCGTCCTTGGGGGAGCCCATCACAACATAAGAGGTGATGGCGCTCACCTGCGGAA
TGGCGCCCAAAGTATCGGTG

Product: citrate lyase, beta subunit, putative

Products: NA

Alternate protein names: (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; L-malyl-CoA/beta-methylmalyl-CoA lyase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSFRLQPTAPARPNRCQLFGPGSRPAIFEKMATSAADVINLDLEDSVAPSDKDTARANIIQAISDIDWGTKTLSVRINGL
DTPYWYKDVVDLLEQAGERLDQIMIPKVGCAGDIYAVDALVTAIEAAKGRNKKITFEVIIESAAGIAHVEEIASASPRMA
AMSLGAADFAASMGMQTTGIGGTQENYYMLREGQKYWSDPWHWAQTAIVAACRTHGVLPVDGPFGDFSDDEGFRAQARRS
ATLGMVGKWAIHPKQIAIANEVFTPSEDAVREAREILAAMEQAKANGEGATVYKGRLVDIASIKQAEVIVRQSEMIAS

Sequences:

>Translated_318_residues
MSFRLQPTAPARPNRCQLFGPGSRPAIFEKMATSAADVINLDLEDSVAPSDKDTARANIIQAISDIDWGTKTLSVRINGL
DTPYWYKDVVDLLEQAGERLDQIMIPKVGCAGDIYAVDALVTAIEAAKGRNKKITFEVIIESAAGIAHVEEIASASPRMA
AMSLGAADFAASMGMQTTGIGGTQENYYMLREGQKYWSDPWHWAQTAIVAACRTHGVLPVDGPFGDFSDDEGFRAQARRS
ATLGMVGKWAIHPKQIAIANEVFTPSEDAVREAREILAAMEQAKANGEGATVYKGRLVDIASIKQAEVIVRQSEMIAS
>Mature_317_residues
SFRLQPTAPARPNRCQLFGPGSRPAIFEKMATSAADVINLDLEDSVAPSDKDTARANIIQAISDIDWGTKTLSVRINGLD
TPYWYKDVVDLLEQAGERLDQIMIPKVGCAGDIYAVDALVTAIEAAKGRNKKITFEVIIESAAGIAHVEEIASASPRMAA
MSLGAADFAASMGMQTTGIGGTQENYYMLREGQKYWSDPWHWAQTAIVAACRTHGVLPVDGPFGDFSDDEGFRAQARRSA
TLGMVGKWAIHPKQIAIANEVFTPSEDAVREAREILAAMEQAKANGEGATVYKGRLVDIASIKQAEVIVRQSEMIAS

Specific function: Catalyzes the reversible condensation of glyoxylate and acetyl-CoA to L-malyl-CoA and the reversible condensation of glyoxylate and propionyl-CoA to beta-methylmalyl-CoA [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=309, Percent_Identity=29.7734627831715, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI87081764, Length=305, Percent_Identity=29.1803278688525, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557458, Length=322, Percent_Identity=26.3975155279503, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.24 [H]

Molecular weight: Translated: 34363; Mature: 34232

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRLQPTAPARPNRCQLFGPGSRPAIFEKMATSAADVINLDLEDSVAPSDKDTARANII
CCEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHH
QAISDIDWGTKTLSVRINGLDTPYWYKDVVDLLEQAGERLDQIMIPKVGCAGDIYAVDAL
HHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VTAIEAAKGRNKKITFEVIIESAAGIAHVEEIASASPRMAAMSLGAADFAASMGMQTTGI
HHHHHHHCCCCCEEEEEEEEEHHCCHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCC
GGTQENYYMLREGQKYWSDPWHWAQTAIVAACRTHGVLPVDGPFGDFSDDEGFRAQARRS
CCCCCCEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH
ATLGMVGKWAIHPKQIAIANEVFTPSEDAVREAREILAAMEQAKANGEGATVYKGRLVDI
HHHCCCEEEEECCCCEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEH
ASIKQAEVIVRQSEMIAS
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SFRLQPTAPARPNRCQLFGPGSRPAIFEKMATSAADVINLDLEDSVAPSDKDTARANII
CEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHH
QAISDIDWGTKTLSVRINGLDTPYWYKDVVDLLEQAGERLDQIMIPKVGCAGDIYAVDAL
HHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VTAIEAAKGRNKKITFEVIIESAAGIAHVEEIASASPRMAAMSLGAADFAASMGMQTTGI
HHHHHHHCCCCCEEEEEEEEEHHCCHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCC
GGTQENYYMLREGQKYWSDPWHWAQTAIVAACRTHGVLPVDGPFGDFSDDEGFRAQARRS
CCCCCCEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH
ATLGMVGKWAIHPKQIAIANEVFTPSEDAVREAREILAAMEQAKANGEGATVYKGRLVDI
HHHCCCEEEEECCCCEEEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEH
ASIKQAEVIVRQSEMIAS
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA