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The map label for this gene is nemA [C]

Identifier: 110678338

GI number: 110678338

Start: 958180

End: 959382

Strand: Direct

Name: nemA [C]

Synonym: RD1_0993

Alternate gene names: 110678338

Gene position: 958180-959382 (Clockwise)

Preceding gene: 110678337

Following gene: 110678340

Centisome position: 23.18

GC content: 61.18

Gene sequence:

>1203_bases
ATGAAGAACCCATTGGCCCAACCGCTTTTTCTGCCCTGCGGCGTTCGGCTCAAGAATCGCATTATCAAATCCGCCATGTC
TGATTCCCTTGGTGATGGTGCGGGCAATCCGACCGGGGCGCAGATGCGTCTTTACGAACGATGGGCCGAGGGAGGGGCTG
CGCTGTCGCTGATTGGTGAAGTGCAGGTTGTCCCGGATTTCCCCGAGAAGCCCGGCAATCTGGTTTTCGGATCGGATGCG
GACCTTCCGGCCCTGCGGGAACTGGCACGGCGCGGCTCCGTCAACGGCGCGCATATCTGGCCGCAGCTGGGCCACGCGGG
GGCGCTGTCACATGCGCCGATCAGCAGGCCCAGAGGCCCCTCGGCACTGGATGTTGCGGGTCTGAAATGCGACGCAATGT
CCGTGGATGACATTCACAACCTGCCCCACGCCTATGCGCAGGCCGCGATGCTGGCACAGAGCGCGGGCTTTGGCGGCGTG
CTGATCCATGCAGGTCACGGATTCCTCCTGAGCCAGTTCCTGTCACCCTTGTTCAATCACCGCACGGATTCCTATGGCGG
CTCGATTAGCGGGCGTTTCCGCATAATACGAGAGGTGCTCAGCGCCGTTCGGCAAGCGGTCGGGGCGGCTTATCCGGTCG
GGATCAGGATCAACGCCACCGACAAACTGGCAGGCGGGCTGACACATCACGACGCGCTGGACGTCGTGCGTTTGTTGGAT
GACACATCCGTGGATTTGATCGACATCAGTGGTGGTACGTATTTCCCCGGCGCCGCATCAAGTTCGGATGAATTAGCGAC
CTCCGGCCCCTATTTCAGCGAGTTTGGCAAGAAAGCCAAATCAGTGACGTCTGTTCCGATCGTTCTGTCGGGTGGGTTTG
AAACGCGCGATCAGGCGCTGAAAGCGCTGCAAGGCGGCGCGGCAGATGCCATCAGCCTCGGTCGTGCCATGGTGCTGAAC
CCTGCGATTGCGAATGTGTGGCAAAGCGCTGCGGGGGGTGATCCGGTCTTTCCGACATTCGTTTTGCCGCCACGTGGTGG
TGTTACCGCATGGTATTCCATGCGCCTGACAGCGCTCGGCACGGACAGTGAGCACCTGTTTGATGCAAGTCCGCTGGCGG
CCCTTGATGCCTATGAGCAACGCGATGCGCAGCGCTGTGACATCTGGCGCAAGTGTTTCGGTGCCCGCCCGCCCGACAGG
TGA

Upstream 100 bases:

>100_bases
ATGAACTGTTGAATCCACGGAAGTAGAGGTGCAGCATATCACATCGAAACACGCGCCTATAAAGTGATATATCGAAATAT
GCCGATAGAAAGATAATGAT

Downstream 100 bases:

>100_bases
TCATTGACTTTGCGCGGGATTAAGTCGTGCGCCGTTTCGCGCCCGAAAGCGCAAACGCTTCAGCATCGCACGAATTCGCG
CGGCCGCATCATCGCAAATT

Product: oxidoreductase, putative

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKNPLAQPLFLPCGVRLKNRIIKSAMSDSLGDGAGNPTGAQMRLYERWAEGGAALSLIGEVQVVPDFPEKPGNLVFGSDA
DLPALRELARRGSVNGAHIWPQLGHAGALSHAPISRPRGPSALDVAGLKCDAMSVDDIHNLPHAYAQAAMLAQSAGFGGV
LIHAGHGFLLSQFLSPLFNHRTDSYGGSISGRFRIIREVLSAVRQAVGAAYPVGIRINATDKLAGGLTHHDALDVVRLLD
DTSVDLIDISGGTYFPGAASSSDELATSGPYFSEFGKKAKSVTSVPIVLSGGFETRDQALKALQGGAADAISLGRAMVLN
PAIANVWQSAAGGDPVFPTFVLPPRGGVTAWYSMRLTALGTDSEHLFDASPLAALDAYEQRDAQRCDIWRKCFGARPPDR

Sequences:

>Translated_400_residues
MKNPLAQPLFLPCGVRLKNRIIKSAMSDSLGDGAGNPTGAQMRLYERWAEGGAALSLIGEVQVVPDFPEKPGNLVFGSDA
DLPALRELARRGSVNGAHIWPQLGHAGALSHAPISRPRGPSALDVAGLKCDAMSVDDIHNLPHAYAQAAMLAQSAGFGGV
LIHAGHGFLLSQFLSPLFNHRTDSYGGSISGRFRIIREVLSAVRQAVGAAYPVGIRINATDKLAGGLTHHDALDVVRLLD
DTSVDLIDISGGTYFPGAASSSDELATSGPYFSEFGKKAKSVTSVPIVLSGGFETRDQALKALQGGAADAISLGRAMVLN
PAIANVWQSAAGGDPVFPTFVLPPRGGVTAWYSMRLTALGTDSEHLFDASPLAALDAYEQRDAQRCDIWRKCFGARPPDR
>Mature_400_residues
MKNPLAQPLFLPCGVRLKNRIIKSAMSDSLGDGAGNPTGAQMRLYERWAEGGAALSLIGEVQVVPDFPEKPGNLVFGSDA
DLPALRELARRGSVNGAHIWPQLGHAGALSHAPISRPRGPSALDVAGLKCDAMSVDDIHNLPHAYAQAAMLAQSAGFGGV
LIHAGHGFLLSQFLSPLFNHRTDSYGGSISGRFRIIREVLSAVRQAVGAAYPVGIRINATDKLAGGLTHHDALDVVRLLD
DTSVDLIDISGGTYFPGAASSSDELATSGPYFSEFGKKAKSVTSVPIVLSGGFETRDQALKALQGGAADAISLGRAMVLN
PAIANVWQSAAGGDPVFPTFVLPPRGGVTAWYSMRLTALGTDSEHLFDASPLAALDAYEQRDAQRCDIWRKCFGARPPDR

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=263, Percent_Identity=30.0380228136882, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789463, Length=321, Percent_Identity=26.4797507788162, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=32.3615160349854, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17559802, Length=343, Percent_Identity=31.1953352769679, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17559804, Length=343, Percent_Identity=31.1953352769679, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17564188, Length=342, Percent_Identity=28.9473684210526, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17540738, Length=344, Percent_Identity=28.4883720930233, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI72001454, Length=334, Percent_Identity=29.6407185628743, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17566914, Length=413, Percent_Identity=24.6973365617433, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17566916, Length=341, Percent_Identity=25.2199413489736, Blast_Score=104, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321973, Length=86, Percent_Identity=45.3488372093023, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=87, Percent_Identity=41.3793103448276, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42170; Mature: 42170

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPLAQPLFLPCGVRLKNRIIKSAMSDSLGDGAGNPTGAQMRLYERWAEGGAALSLIGE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
VQVVPDFPEKPGNLVFGSDADLPALRELARRGSVNGAHIWPQLGHAGALSHAPISRPRGP
EEECCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCC
SALDVAGLKCDAMSVDDIHNLPHAYAQAAMLAQSAGFGGVLIHAGHGFLLSQFLSPLFNH
CCEEECCCEECCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHC
RTDSYGGSISGRFRIIREVLSAVRQAVGAAYPVGIRINATDKLAGGLTHHDALDVVRLLD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCHHHHHHHHHHHC
DTSVDLIDISGGTYFPGAASSSDELATSGPYFSEFGKKAKSVTSVPIVLSGGFETRDQAL
CCCEEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
KALQGGAADAISLGRAMVLNPAIANVWQSAAGGDPVFPTFVLPPRGGVTAWYSMRLTALG
HHHHCCCHHHHHHCHHHEECHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEEEEEEC
TDSEHLFDASPLAALDAYEQRDAQRCDIWRKCFGARPPDR
CCCHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKNPLAQPLFLPCGVRLKNRIIKSAMSDSLGDGAGNPTGAQMRLYERWAEGGAALSLIGE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
VQVVPDFPEKPGNLVFGSDADLPALRELARRGSVNGAHIWPQLGHAGALSHAPISRPRGP
EEECCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCC
SALDVAGLKCDAMSVDDIHNLPHAYAQAAMLAQSAGFGGVLIHAGHGFLLSQFLSPLFNH
CCEEECCCEECCCCHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHC
RTDSYGGSISGRFRIIREVLSAVRQAVGAAYPVGIRINATDKLAGGLTHHDALDVVRLLD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCHHHHHHHHHHHC
DTSVDLIDISGGTYFPGAASSSDELATSGPYFSEFGKKAKSVTSVPIVLSGGFETRDQAL
CCCEEEEECCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHH
KALQGGAADAISLGRAMVLNPAIANVWQSAAGGDPVFPTFVLPPRGGVTAWYSMRLTALG
HHHHCCCHHHHHHCHHHEECHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEEEEEEEEEC
TDSEHLFDASPLAALDAYEQRDAQRCDIWRKCFGARPPDR
CCCHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]