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The map label for this gene is yafC [C]

Identifier: 110678302

GI number: 110678302

Start: 922375

End: 923283

Strand: Reverse

Name: yafC [C]

Synonym: RD1_0955

Alternate gene names: 110678302

Gene position: 923283-922375 (Counterclockwise)

Preceding gene: 110678304

Following gene: 110678297

Centisome position: 22.34

GC content: 56.11

Gene sequence:

>909_bases
GTGATGGACCGGCTCACGGAAATGGAAGCCTTTGCGATGGTGGTGGATCAGGGTGGCTTTACCGATGCCGCCAAGAAACT
GGGCATCTCGAAATCCGCCGTCTCGAAACATGTGTCCTCGCTTGAGGCGCGGCTCGGCGCACGGCTTTTGAACAGAACGA
CGCGCCGGGTCAGCCCGACCGAAATCGGTCTTGCCTATTATGACCGGGCCCGCCGCGTTTTGAACGACGCGGGCGAAGCA
GACGCGCTGGTGACATCCATGCAGAGCGCGCCTTCGGGACTGCTGCGGATTTCTGTCGCAACTGATTTTGGTGTGAACCA
TCTGTCGCCCGTTCTCAGCGAATTTTTATCGGATTTCCCGGATATCACGGTGAACATGGTGCTCAACAATCGCTACGTCG
AACTGATCTCGGAAGGGTTCGACATGGCCGTGCGGATAGGCGAGCTTGAGGACAGCACATTGCGCGCGCGCAAACTGACC
GAGACGACCAAACGGATGATCGCCAGCCCGGGGTACTTTGAAAAATATGGCCGCCCGGAAAAAATCGACGACCTGAACGC
GCATAAACTGCTGCATTACTCCAATCAGTCATCAGGTAACGTCTGGAAAATCACTGCGCCATCGGGTGAAAAGCGTCAGG
TCCGCACCGCTGGCTGGCTCAGCGTCAATGACGGTCAATCCTTGCTGAACGCAGCCATTTCAGGGCTGGGGATCGCCTAT
CTTCCCAGTTTTCTATACGCAGAAGCGATGGAAAAAGGTCTCGTCGAAGATGCCATTCCCGACCTCCCGATGGAGATGCA
GGGCATCTATGCGGTCTATCCTCCAGGTCGCTTCACCCAACCCAAGGTCCGCGCCTTCATCGATTTTCTGGTCAATGCCT
TTGCCGAAAAAGGACCGACAGAATGGTAA

Upstream 100 bases:

>100_bases
GCGAACGATCCACCCAAAAAACGTGATACTGTCCCAGAGCTGCCGCGTTCCTGCCAGAATTGCCCCGCATAAAAGAGTCC
GAAGCAATTCGAAGGGAAGA

Downstream 100 bases:

>100_bases
GGTGACAGCCTGACCAACGCGGGGCGTCCCAGAGCGGACGGCGCAGTTTCATTGCGTGTTGAGCAGAACGACCTCAACCC
GGCGGTTCAACCTGCGCCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MMDRLTEMEAFAMVVDQGGFTDAAKKLGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEA
DALVTSMQSAPSGLLRISVATDFGVNHLSPVLSEFLSDFPDITVNMVLNNRYVELISEGFDMAVRIGELEDSTLRARKLT
ETTKRMIASPGYFEKYGRPEKIDDLNAHKLLHYSNQSSGNVWKITAPSGEKRQVRTAGWLSVNDGQSLLNAAISGLGIAY
LPSFLYAEAMEKGLVEDAIPDLPMEMQGIYAVYPPGRFTQPKVRAFIDFLVNAFAEKGPTEW

Sequences:

>Translated_302_residues
MMDRLTEMEAFAMVVDQGGFTDAAKKLGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEA
DALVTSMQSAPSGLLRISVATDFGVNHLSPVLSEFLSDFPDITVNMVLNNRYVELISEGFDMAVRIGELEDSTLRARKLT
ETTKRMIASPGYFEKYGRPEKIDDLNAHKLLHYSNQSSGNVWKITAPSGEKRQVRTAGWLSVNDGQSLLNAAISGLGIAY
LPSFLYAEAMEKGLVEDAIPDLPMEMQGIYAVYPPGRFTQPKVRAFIDFLVNAFAEKGPTEW
>Mature_302_residues
MMDRLTEMEAFAMVVDQGGFTDAAKKLGISKSAVSKHVSSLEARLGARLLNRTTRRVSPTEIGLAYYDRARRVLNDAGEA
DALVTSMQSAPSGLLRISVATDFGVNHLSPVLSEFLSDFPDITVNMVLNNRYVELISEGFDMAVRIGELEDSTLRARKLT
ETTKRMIASPGYFEKYGRPEKIDDLNAHKLLHYSNQSSGNVWKITAPSGEKRQVRTAGWLSVNDGQSLLNAAISGLGIAY
LPSFLYAEAMEKGLVEDAIPDLPMEMQGIYAVYPPGRFTQPKVRAFIDFLVNAFAEKGPTEW

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=38.8111888111888, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=33.6700336700337, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=32.7702702702703, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=31.3131313131313, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=28.0701754385965, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1787589, Length=295, Percent_Identity=32.2033898305085, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=28.4722222222222, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=26.2068965517241, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=24.6575342465753, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=25.1700680272109, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790011, Length=247, Percent_Identity=25.1012145748988, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=297, Percent_Identity=24.9158249158249, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=22.742474916388, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=25.5892255892256, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=30.1724137931034, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33318; Mature: 33318

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDRLTEMEAFAMVVDQGGFTDAAKKLGISKSAVSKHVSSLEARLGARLLNRTTRRVSPT
CCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EIGLAYYDRARRVLNDAGEADALVTSMQSAPSGLLRISVATDFGVNHLSPVLSEFLSDFP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
DITVNMVLNNRYVELISEGFDMAVRIGELEDSTLRARKLTETTKRMIASPGYFEKYGRPE
CEEEEEEECHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
KIDDLNAHKLLHYSNQSSGNVWKITAPSGEKRQVRTAGWLSVNDGQSLLNAAISGLGIAY
CCCCCCHHHHEEECCCCCCCEEEEECCCCCCCEEEEECCEEECCCHHHHHHHHHHCHHHH
LPSFLYAEAMEKGLVEDAIPDLPMEMQGIYAVYPPGRFTQPKVRAFIDFLVNAFAEKGPT
HHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
EW
CC
>Mature Secondary Structure
MMDRLTEMEAFAMVVDQGGFTDAAKKLGISKSAVSKHVSSLEARLGARLLNRTTRRVSPT
CCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EIGLAYYDRARRVLNDAGEADALVTSMQSAPSGLLRISVATDFGVNHLSPVLSEFLSDFP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCC
DITVNMVLNNRYVELISEGFDMAVRIGELEDSTLRARKLTETTKRMIASPGYFEKYGRPE
CEEEEEEECHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
KIDDLNAHKLLHYSNQSSGNVWKITAPSGEKRQVRTAGWLSVNDGQSLLNAAISGLGIAY
CCCCCCHHHHEEECCCCCCCEEEEECCCCCCCEEEEECCEEECCCHHHHHHHHHHCHHHH
LPSFLYAEAMEKGLVEDAIPDLPMEMQGIYAVYPPGRFTQPKVRAFIDFLVNAFAEKGPT
HHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
EW
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]