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The map label for this gene is thiF [H]

Identifier: 110678114

GI number: 110678114

Start: 723493

End: 724449

Strand: Direct

Name: thiF [H]

Synonym: RD1_0751

Alternate gene names: 110678114

Gene position: 723493-724449 (Clockwise)

Preceding gene: 110678113

Following gene: 110678115

Centisome position: 17.5

GC content: 60.92

Gene sequence:

>957_bases
ATGAGCCGCTATGCCCGCCAGACTGCGGTATTGGGACAGGCCGCGCAGGATAAGCTGGCCGGGTCCTCTGTCCTTGTGAT
CGGTGGCGGCGGTTTGGCCGCCCCCGTGATGCAATACCTCGTCGGTGCCGGTGTCGGCCAAATCCGAATTGCGGATGCAG
ATGTCATAAGCCTGTCCAACCTGCACCGCCAAACGCTGTTTCGCGAGGCTGATATCGGCTTGCCAAAAGTCGATGTGATC
GCAAAGGCGATGGCTGCGTTAAATCCTGACACGCGCATTGAACCCATTCGCCAGCGCGTCGACCCGGCCAATATTGCAGC
CCTTTGCGAGGGCATGACACTGACCCTGGACTGTGCAGACAGTTTCGCGGTCAGCTATATCGCGTCTGATTATTGTCTTG
GGGCAGGTCAGCCCCTTGTCAGCGCCAGCGTAGTGGGGCGCGACGGTTATGTCGGCGGCTTTTGTGGCGGCAAACCGGGG
TTGCGTGCGGTTTTTCCCGATCTGCCGGATCAGCTTGGCTCTTGTGATACAGACGGTGTACTGGGGCCAAGTGTCGGTGT
GATCGGCAGCCTTCAGGCGCAGATGGCGATCGCAGTCCTGACGGGCGATCTGCACGTCTTGGGGCAGCTTGTAACCTTTG
ACGCGGGCGGGATGCGGTTTGGGGGCTTCCGCTTTGACACGGCATCAGACCCTGCGCCCAATCCCGCCTTTATCGCCCCG
CGCGACATCGCCGCGACTGATTTTCTGATCGACCTGCGCGCGAAAGATGAAAAGGGGCCAATCCTACCGCAGGCAACGCG
CCATGCCGTGTCCGATCTTGGGCCACATGGCCCCACACCTGACGCAAACCAACGTGCCGTTCTGGCCTGCCGTTCTGGCC
TGCGTGCCTGGCAGGCGGCGGACCGGCTTTCACAACACTGGCAGGGCGAGATCAAACTTCTCGCTCTCGGAGAATAA

Upstream 100 bases:

>100_bases
CGGCATTTGATGCCGGCGCGGATGTTGTCTCGGCCGTTACGGACATTGCGCTGCACGACGATCCGGAGAGCCGCGTCAGG
GAATGGCTGGAGGTCTGCGG

Downstream 100 bases:

>100_bases
GGAAACCCCCATGAAATACATCATCACCGCAGCGGCGCTATGTGTCGCGACACCAGCCTTCGCTCAAGACAAGATGACCC
TGCTGCTGGATTGGTTCATA

Product: thiamine biosynthesis protein ThiF, putative

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSRYARQTAVLGQAAQDKLAGSSVLVIGGGGLAAPVMQYLVGAGVGQIRIADADVISLSNLHRQTLFREADIGLPKVDVI
AKAMAALNPDTRIEPIRQRVDPANIAALCEGMTLTLDCADSFAVSYIASDYCLGAGQPLVSASVVGRDGYVGGFCGGKPG
LRAVFPDLPDQLGSCDTDGVLGPSVGVIGSLQAQMAIAVLTGDLHVLGQLVTFDAGGMRFGGFRFDTASDPAPNPAFIAP
RDIAATDFLIDLRAKDEKGPILPQATRHAVSDLGPHGPTPDANQRAVLACRSGLRAWQAADRLSQHWQGEIKLLALGE

Sequences:

>Translated_318_residues
MSRYARQTAVLGQAAQDKLAGSSVLVIGGGGLAAPVMQYLVGAGVGQIRIADADVISLSNLHRQTLFREADIGLPKVDVI
AKAMAALNPDTRIEPIRQRVDPANIAALCEGMTLTLDCADSFAVSYIASDYCLGAGQPLVSASVVGRDGYVGGFCGGKPG
LRAVFPDLPDQLGSCDTDGVLGPSVGVIGSLQAQMAIAVLTGDLHVLGQLVTFDAGGMRFGGFRFDTASDPAPNPAFIAP
RDIAATDFLIDLRAKDEKGPILPQATRHAVSDLGPHGPTPDANQRAVLACRSGLRAWQAADRLSQHWQGEIKLLALGE
>Mature_317_residues
SRYARQTAVLGQAAQDKLAGSSVLVIGGGGLAAPVMQYLVGAGVGQIRIADADVISLSNLHRQTLFREADIGLPKVDVIA
KAMAALNPDTRIEPIRQRVDPANIAALCEGMTLTLDCADSFAVSYIASDYCLGAGQPLVSASVVGRDGYVGGFCGGKPGL
RAVFPDLPDQLGSCDTDGVLGPSVGVIGSLQAQMAIAVLTGDLHVLGQLVTFDAGGMRFGGFRFDTASDPAPNPAFIAPR
DIAATDFLIDLRAKDEKGPILPQATRHAVSDLGPHGPTPDANQRAVLACRSGLRAWQAADRLSQHWQGEIKLLALGE

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=170, Percent_Identity=37.6470588235294, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI38045948, Length=165, Percent_Identity=33.9393939393939, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4885649, Length=161, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI23510338, Length=159, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI23510340, Length=159, Percent_Identity=28.3018867924528, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787048, Length=230, Percent_Identity=37.8260869565217, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI87082356, Length=231, Percent_Identity=36.3636363636364, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789177, Length=140, Percent_Identity=27.1428571428571, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=252, Percent_Identity=36.1111111111111, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI86565433, Length=104, Percent_Identity=40.3846153846154, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI86565431, Length=104, Percent_Identity=40.3846153846154, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=207, Percent_Identity=36.7149758454106, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320598, Length=160, Percent_Identity=28.75, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322639, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24582879, Length=282, Percent_Identity=34.0425531914894, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24660640, Length=137, Percent_Identity=32.8467153284672, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28573937, Length=158, Percent_Identity=31.0126582278481, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 33078; Mature: 32947

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYARQTAVLGQAAQDKLAGSSVLVIGGGGLAAPVMQYLVGAGVGQIRIADADVISLSN
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHH
LHRQTLFREADIGLPKVDVIAKAMAALNPDTRIEPIRQRVDPANIAALCEGMTLTLDCAD
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCEEEEECCH
SFAVSYIASDYCLGAGQPLVSASVVGRDGYVGGFCGGKPGLRAVFPDLPDQLGSCDTDGV
HHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHCCCCCCCC
LGPSVGVIGSLQAQMAIAVLTGDLHVLGQLVTFDAGGMRFGGFRFDTASDPAPNPAFIAP
CCCCCCHHHHHHHHEEEEEEECCHHHHHHHHEECCCCCEECCEEECCCCCCCCCCCEECC
RDIAATDFLIDLRAKDEKGPILPQATRHAVSDLGPHGPTPDANQRAVLACRSGLRAWQAA
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DRLSQHWQGEIKLLALGE
HHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
SRYARQTAVLGQAAQDKLAGSSVLVIGGGGLAAPVMQYLVGAGVGQIRIADADVISLSN
CHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCHHHHH
LHRQTLFREADIGLPKVDVIAKAMAALNPDTRIEPIRQRVDPANIAALCEGMTLTLDCAD
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHCCCEEEEECCH
SFAVSYIASDYCLGAGQPLVSASVVGRDGYVGGFCGGKPGLRAVFPDLPDQLGSCDTDGV
HHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCCCCCCCCEECCCCCCHHHCCCCCCCC
LGPSVGVIGSLQAQMAIAVLTGDLHVLGQLVTFDAGGMRFGGFRFDTASDPAPNPAFIAP
CCCCCCHHHHHHHHEEEEEEECCHHHHHHHHEECCCCCEECCEEECCCCCCCCCCCEECC
RDIAATDFLIDLRAKDEKGPILPQATRHAVSDLGPHGPTPDANQRAVLACRSGLRAWQAA
CCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DRLSQHWQGEIKLLALGE
HHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]