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The map label for this gene is ilvD [H]

Identifier: 110678062

GI number: 110678062

Start: 666225

End: 668024

Strand: Direct

Name: ilvD [H]

Synonym: RD1_0686

Alternate gene names: 110678062

Gene position: 666225-668024 (Clockwise)

Preceding gene: 110678061

Following gene: 110678063

Centisome position: 16.12

GC content: 59.67

Gene sequence:

>1800_bases
ATGACAAAGAAAATCGGTATCGAAGACTTGCGGTCGCGCAAGTGGTTCATGAACCCGGACAATCCGGAAATGACCGCGCT
TTATCTTGAGCGCTATCTCAATTACGGGCTGACGCGGGGCGAGTTACAATCGGGCAAGCCGATCATCGGTATTGCGCAAA
CCGGCAGTGACCTCAGCCCCTGTAACCGCCATCACCTCGAGTTGACCAAGCGCGTACGCGACGGGATCATTGCGGCAGGC
GGCACCTGCATCGAGGTGCCCGTGCACCCCATTCAGGAAACCGGCAAACGCCCTACGGCGATGCTGGATCGCAACCTTGC
CTATCTGTCGCTGGTTGAGACGCTGTTTGGCTACCCGCTGGATGGTGTTGTGCTGAATATCGGCTGTGACAAGACCACGC
CTGCGCTTTTGATGGCGGCTGCGACGGTGAACATTCCTGCGATTGCCCTGTCGGTGGGGCCAATGCTGAACGGCTGGTTC
AAGGGTGAGCGGACAGGGTCCGGGACAATCGTCTGGAAGGCGCGCGAAATGCTGGCCGCCGGAGAGATTGATGACGACGG
TTTCATGGAGCTTGTTGCCTCTTCCGCGCCGTCGGTGGGCTATTGCAACACGATGGGCACCGCGACCACGATGAATTCAC
TGGCGGAGGCCTTGGGCATGCAACTGCCCGGTTCCGCGGCGATCCCGGCACCCTACCGCGAGCGGGGACAGATCAGCTAT
GAAACCGGGCGGCGCATTGTGGACATGGTCTGGGAAGACCTGCGCCCCTCGCAGATCATGACGCGCGAGGCTTTCGAGAA
CGCGATTGTCATCAATTCCGCCATTGGGGGGTCGACGAATGCGCCGATCCACCTGAACGGGATCGCGCGCCATCTGGGTG
TTCCGCTGGACAATAACGATTGGCAGAAGCTGGGCCATGATGTGCCGTTGTTGGTCAACTTGCAGCCGGCGGGTGAATAC
CTTGGCGAAGACTACAGCCATGCGGGCGGGGTGCCTGCCGTCGTGGGTGAGTTGATGGCAGAGGGCATGTTGCCGCATCC
CGACGTGATCACCGTGAATGGCAAAACCATGGGCGAGAACTGCGCCGATATGCGCAGCGCGGATACCAAGGTGATCAAGA
CCGTTGCGGATCCATTGAAAGCGCAGGCGGGTTTTATCAACCTGACCGGCAATCTTTTTGACAGTGCGATCATGAAGACC
AGCGTGATCAGCGAGAGTTTTCGCGCCAAATATCTGTCGAACCCTGAGGATCCGAATGCTTTTGAGGGGCGTGCGGTTGT
CTTTGACGGGCCGGAGGATTTTCACAACCGCATCGACGATCCCGCCGAGGCGATCGACGAAAACTGCATCCTCTTCATGC
GCGGTGCAGGCCCAAAGGGGTATCCCGGTGGGGCTGAGGTGGTGAACATGCGCCCCCCGTCCTATCTGATCAAACAGGGT
GTTGAAGCCTTGCCCTGCGTGGGTGACGGGCGGCAATCGGGAACCTCGGGCAGCCCATCGATCCTGAACGCCTCCCCTGA
GGCGGCGGCGGGTGGTGGTCTTGCGCTGTTGCAAAACGGGGACCCTGTGCGCATCGATTTGCAAAAATGCACCGCCAACA
GCCTCGTGCCGCTGGATGAACTGGCGGAACGCGCCCGCTTGTTCGAGGCTAATGGCGGCTACGACGCCCCCGAAAGCCAG
TCGCCCTGGCAGCAATATTTCCGCGAAAAAGTCGGCCGTTTTGACGAGGGAATGATCCTCGAAGATGCCGACAAATATCA
GGACATCTCGCGTCGGTTCATGCCGCGCGACAACCACTAG

Upstream 100 bases:

>100_bases
GTGCCGATGAGATCGCCTCGCTCGTCGTCTATCTTGCCAGCGATGAAAGCGCGTTCACCACAGGGCATCCCCATATCATT
GACGGAGGCTGGTCAGGCCA

Downstream 100 bases:

>100_bases
GCGCTGCGGCGCACAGACGAAAAGGAAGAAGACATGAAACTTGTAAGATACGGTGACGCAGGCGCAGAAAAACCCGGCAT
GCTGGATGCAGAAGGCGCGT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MTKKIGIEDLRSRKWFMNPDNPEMTALYLERYLNYGLTRGELQSGKPIIGIAQTGSDLSPCNRHHLELTKRVRDGIIAAG
GTCIEVPVHPIQETGKRPTAMLDRNLAYLSLVETLFGYPLDGVVLNIGCDKTTPALLMAAATVNIPAIALSVGPMLNGWF
KGERTGSGTIVWKAREMLAAGEIDDDGFMELVASSAPSVGYCNTMGTATTMNSLAEALGMQLPGSAAIPAPYRERGQISY
ETGRRIVDMVWEDLRPSQIMTREAFENAIVINSAIGGSTNAPIHLNGIARHLGVPLDNNDWQKLGHDVPLLVNLQPAGEY
LGEDYSHAGGVPAVVGELMAEGMLPHPDVITVNGKTMGENCADMRSADTKVIKTVADPLKAQAGFINLTGNLFDSAIMKT
SVISESFRAKYLSNPEDPNAFEGRAVVFDGPEDFHNRIDDPAEAIDENCILFMRGAGPKGYPGGAEVVNMRPPSYLIKQG
VEALPCVGDGRQSGTSGSPSILNASPEAAAGGGLALLQNGDPVRIDLQKCTANSLVPLDELAERARLFEANGGYDAPESQ
SPWQQYFREKVGRFDEGMILEDADKYQDISRRFMPRDNH

Sequences:

>Translated_599_residues
MTKKIGIEDLRSRKWFMNPDNPEMTALYLERYLNYGLTRGELQSGKPIIGIAQTGSDLSPCNRHHLELTKRVRDGIIAAG
GTCIEVPVHPIQETGKRPTAMLDRNLAYLSLVETLFGYPLDGVVLNIGCDKTTPALLMAAATVNIPAIALSVGPMLNGWF
KGERTGSGTIVWKAREMLAAGEIDDDGFMELVASSAPSVGYCNTMGTATTMNSLAEALGMQLPGSAAIPAPYRERGQISY
ETGRRIVDMVWEDLRPSQIMTREAFENAIVINSAIGGSTNAPIHLNGIARHLGVPLDNNDWQKLGHDVPLLVNLQPAGEY
LGEDYSHAGGVPAVVGELMAEGMLPHPDVITVNGKTMGENCADMRSADTKVIKTVADPLKAQAGFINLTGNLFDSAIMKT
SVISESFRAKYLSNPEDPNAFEGRAVVFDGPEDFHNRIDDPAEAIDENCILFMRGAGPKGYPGGAEVVNMRPPSYLIKQG
VEALPCVGDGRQSGTSGSPSILNASPEAAAGGGLALLQNGDPVRIDLQKCTANSLVPLDELAERARLFEANGGYDAPESQ
SPWQQYFREKVGRFDEGMILEDADKYQDISRRFMPRDNH
>Mature_598_residues
TKKIGIEDLRSRKWFMNPDNPEMTALYLERYLNYGLTRGELQSGKPIIGIAQTGSDLSPCNRHHLELTKRVRDGIIAAGG
TCIEVPVHPIQETGKRPTAMLDRNLAYLSLVETLFGYPLDGVVLNIGCDKTTPALLMAAATVNIPAIALSVGPMLNGWFK
GERTGSGTIVWKAREMLAAGEIDDDGFMELVASSAPSVGYCNTMGTATTMNSLAEALGMQLPGSAAIPAPYRERGQISYE
TGRRIVDMVWEDLRPSQIMTREAFENAIVINSAIGGSTNAPIHLNGIARHLGVPLDNNDWQKLGHDVPLLVNLQPAGEYL
GEDYSHAGGVPAVVGELMAEGMLPHPDVITVNGKTMGENCADMRSADTKVIKTVADPLKAQAGFINLTGNLFDSAIMKTS
VISESFRAKYLSNPEDPNAFEGRAVVFDGPEDFHNRIDDPAEAIDENCILFMRGAGPKGYPGGAEVVNMRPPSYLIKQGV
EALPCVGDGRQSGTSGSPSILNASPEAAAGGGLALLQNGDPVRIDLQKCTANSLVPLDELAERARLFEANGGYDAPESQS
PWQQYFREKVGRFDEGMILEDADKYQDISRRFMPRDNH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=587, Percent_Identity=28.9608177172061, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1788157, Length=464, Percent_Identity=31.25, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI2367371, Length=462, Percent_Identity=27.7056277056277, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786464, Length=457, Percent_Identity=28.4463894967177, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6322476, Length=523, Percent_Identity=29.4455066921606, Blast_Score=188, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 64703; Mature: 64572

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIGIEDLRSRKWFMNPDNPEMTALYLERYLNYGLTRGELQSGKPIIGIAQTGSDLSP
CCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCC
CNRHHLELTKRVRDGIIAAGGTCIEVPVHPIQETGKRPTAMLDRNLAYLSLVETLFGYPL
CHHHHHHHHHHHHCCEEECCCEEEECCCCHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCC
DGVVLNIGCDKTTPALLMAAATVNIPAIALSVGPMLNGWFKGERTGSGTIVWKAREMLAA
CCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHC
GEIDDDGFMELVASSAPSVGYCNTMGTATTMNSLAEALGMQLPGSAAIPAPYRERGQISY
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEEH
ETGRRIVDMVWEDLRPSQIMTREAFENAIVINSAIGGSTNAPIHLNGIARHLGVPLDNND
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHCCCCCCCH
WQKLGHDVPLLVNLQPAGEYLGEDYSHAGGVPAVVGELMAEGMLPHPDVITVNGKTMGEN
HHHHCCCCCEEEEECCHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
CADMRSADTKVIKTVADPLKAQAGFINLTGNLFDSAIMKTSVISESFRAKYLSNPEDPNA
HHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FEGRAVVFDGPEDFHNRIDDPAEAIDENCILFMRGAGPKGYPGGAEVVNMRPPSYLIKQG
CCCCEEEECCCHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHCC
VEALPCVGDGRQSGTSGSPSILNASPEAAAGGGLALLQNGDPVRIDLQKCTANSLVPLDE
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHH
LAERARLFEANGGYDAPESQSPWQQYFREKVGRFDEGMILEDADKYQDISRRFMPRDNH
HHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKKIGIEDLRSRKWFMNPDNPEMTALYLERYLNYGLTRGELQSGKPIIGIAQTGSDLSP
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCC
CNRHHLELTKRVRDGIIAAGGTCIEVPVHPIQETGKRPTAMLDRNLAYLSLVETLFGYPL
CHHHHHHHHHHHHCCEEECCCEEEECCCCHHHHCCCCCHHHHHCCHHHHHHHHHHHCCCC
DGVVLNIGCDKTTPALLMAAATVNIPAIALSVGPMLNGWFKGERTGSGTIVWKAREMLAA
CCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCEEEEEHHHHHHC
GEIDDDGFMELVASSAPSVGYCNTMGTATTMNSLAEALGMQLPGSAAIPAPYRERGQISY
CCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCEEH
ETGRRIVDMVWEDLRPSQIMTREAFENAIVINSAIGGSTNAPIHLNGIARHLGVPLDNND
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECCHHHHHCCCCCCCH
WQKLGHDVPLLVNLQPAGEYLGEDYSHAGGVPAVVGELMAEGMLPHPDVITVNGKTMGEN
HHHHCCCCCEEEEECCHHHHHCCCHHCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCC
CADMRSADTKVIKTVADPLKAQAGFINLTGNLFDSAIMKTSVISESFRAKYLSNPEDPNA
HHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FEGRAVVFDGPEDFHNRIDDPAEAIDENCILFMRGAGPKGYPGGAEVVNMRPPSYLIKQG
CCCCEEEECCCHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHCC
VEALPCVGDGRQSGTSGSPSILNASPEAAAGGGLALLQNGDPVRIDLQKCTANSLVPLDE
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHH
LAERARLFEANGGYDAPESQSPWQQYFREKVGRFDEGMILEDADKYQDISRRFMPRDNH
HHHHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]