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The map label for this gene is purH

Identifier: 110678032

GI number: 110678032

Start: 634384

End: 635970

Strand: Direct

Name: purH

Synonym: RD1_0655

Alternate gene names: 110678032

Gene position: 634384-635970 (Clockwise)

Preceding gene: 110678031

Following gene: 110678033

Centisome position: 15.35

GC content: 62.19

Gene sequence:

>1587_bases
ATGACCGACCTTGCCCCGCTGCGCCGCGCGCTTCTTTCCGTTTCAGACAAGACCGGGCTGGTGGCGCTGGGGAAGGCGCT
GGCCGCGCGCGGTGTCGAGCTTTTGTCGACCGGGGGCACGGCGCGGGCGCTGCGCGACGCCGGGCTTGAGGTCAAGGATG
TGGCCGAGGTCACGGGATTTCCGGAAATGATGGACGGGCGGGTCAAGACGCTGCACCCGGCGGTTCACGGCGGTTTGCTG
GCGCTGCGCGACAATGACGATCACCTCGCGGCCATGCAGGAACATGGGATCGCGGGCATAGATCTGGTGGTTGTGAACCT
CTATCCGTTTGAGCAGACGGTCGCCAAGGGCGCGCAATACGACGAAGTCATTGAAAACATTGATATCGGTGGGCCAGCGA
TGATCCGGTCTGCTGCCAAGAACCACGGGTTTGTCAGCGTGGTGGTGGATGTGGAGGATTACGATGTCCTGATCGCGCAA
CTGGAGGCGTATGACGGGCACACGACCTATGCCCTGCGCCAGCGCCTTGCCCAGACCGCATATGCGCGCACCGCTGCCTA
TGATACGGCGGTGAGCACATGGATGGCCGCGCAGGTGGGTGAACCGCCACGGCGACGCAGCTTTGGGGGCACGCTGGCGC
AAACACTGCGGTATGGCGAGAATCCACATCAGGATGCGGCGTTTTACCTCGATGGATCGAACAGCCCCGGCGTTGCCACC
GCGCGCCAGCTTCAGGGCAAGGAGCTGTCATATAACAATATCAATGACACGGATGCGGCCTTTGAGCTTGTCAGTGAATT
TGACCAGGGGGATGGGCCTGCCTGTGCCATCATCAAACATGCAAATCCTTGCGGCGTGGCGCGGGCCGAGACACTGACTG
AGGCGTACAGGCGCGCCTTTGATTGTGACCGCACCTCTGCATTCGGGGGCATCATTGCGCTCAACCAACCGCTGGACGGG
GCGACCGCCGAAGAGATTTCGTCGATTTTTACCGAAGTTGTCATCGCGCCGGGTGCGGACGCAGAGGCACAAGAGGTTTT
TGCCAAAAAGAAGAACCTGCGCCTGCTCACCACGGACGGGCTGGCGGACCCCACAGCGCCGGGTCTGGCGATCCGGCAGG
TGTCGGGGGGGTATCTGGTGCAGGACAGGGATACCGGTCGCTTGTCGGCCGATGCGCTTCAAATCGTCACCAAGCGTGCG
CCAAGCGATCAGGAAATGGCCGATCTGATGTTTGCCTGGACCGTCGCCAAACACGTCAAATCCAATGCCATCATCTATGT
CAAAGACGGCGCGACCGTCGGCGTCGGTGCTGGGCAGATGAGCCGGGTCGACAGCACGAGAATCGCCGCCCGCAAGGCAC
AGGACATGGCCGAGGCGATGGGTCTGCCTGCGCCACTGACACAAGGGTCGGTCGTGGCCTCTGATGCGTTTTTTCCCTTT
GCCGACGGGTTGATCACAGCAGCCGAGGCCGGGGCGACCGCGCTTATCCAGCCGGGTGGATCGATGCGCGATGATGAGGT
TATCGCCGCCGCGGATGAGGCAGGTCTCGCGATGGTGTTTACCGGCATGCGTCACTTCAGGCACTGA

Upstream 100 bases:

>100_bases
AGGTGGTCTTTGTCCAAGGCGCAGGAAACTGCGATCGGGGTGCGGGACCTGAGCCGAGATACCCTGAATTACACCGATTA
GATCGCCCAAAGGACCAGAT

Downstream 100 bases:

>100_bases
ACAAGGGGGCCGCCATGCGTTTGCTCTATGGGGCTGCCGTGATCGCGTTTCTGGTGGATCAGGTCAGCAAATACATCGTG
ATTCACATGATGGAATTGTG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MTDLAPLRRALLSVSDKTGLVALGKALAARGVELLSTGGTARALRDAGLEVKDVAEVTGFPEMMDGRVKTLHPAVHGGLL
ALRDNDDHLAAMQEHGIAGIDLVVVNLYPFEQTVAKGAQYDEVIENIDIGGPAMIRSAAKNHGFVSVVVDVEDYDVLIAQ
LEAYDGHTTYALRQRLAQTAYARTAAYDTAVSTWMAAQVGEPPRRRSFGGTLAQTLRYGENPHQDAAFYLDGSNSPGVAT
ARQLQGKELSYNNINDTDAAFELVSEFDQGDGPACAIIKHANPCGVARAETLTEAYRRAFDCDRTSAFGGIIALNQPLDG
ATAEEISSIFTEVVIAPGADAEAQEVFAKKKNLRLLTTDGLADPTAPGLAIRQVSGGYLVQDRDTGRLSADALQIVTKRA
PSDQEMADLMFAWTVAKHVKSNAIIYVKDGATVGVGAGQMSRVDSTRIAARKAQDMAEAMGLPAPLTQGSVVASDAFFPF
ADGLITAAEAGATALIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH

Sequences:

>Translated_528_residues
MTDLAPLRRALLSVSDKTGLVALGKALAARGVELLSTGGTARALRDAGLEVKDVAEVTGFPEMMDGRVKTLHPAVHGGLL
ALRDNDDHLAAMQEHGIAGIDLVVVNLYPFEQTVAKGAQYDEVIENIDIGGPAMIRSAAKNHGFVSVVVDVEDYDVLIAQ
LEAYDGHTTYALRQRLAQTAYARTAAYDTAVSTWMAAQVGEPPRRRSFGGTLAQTLRYGENPHQDAAFYLDGSNSPGVAT
ARQLQGKELSYNNINDTDAAFELVSEFDQGDGPACAIIKHANPCGVARAETLTEAYRRAFDCDRTSAFGGIIALNQPLDG
ATAEEISSIFTEVVIAPGADAEAQEVFAKKKNLRLLTTDGLADPTAPGLAIRQVSGGYLVQDRDTGRLSADALQIVTKRA
PSDQEMADLMFAWTVAKHVKSNAIIYVKDGATVGVGAGQMSRVDSTRIAARKAQDMAEAMGLPAPLTQGSVVASDAFFPF
ADGLITAAEAGATALIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH
>Mature_527_residues
TDLAPLRRALLSVSDKTGLVALGKALAARGVELLSTGGTARALRDAGLEVKDVAEVTGFPEMMDGRVKTLHPAVHGGLLA
LRDNDDHLAAMQEHGIAGIDLVVVNLYPFEQTVAKGAQYDEVIENIDIGGPAMIRSAAKNHGFVSVVVDVEDYDVLIAQL
EAYDGHTTYALRQRLAQTAYARTAAYDTAVSTWMAAQVGEPPRRRSFGGTLAQTLRYGENPHQDAAFYLDGSNSPGVATA
RQLQGKELSYNNINDTDAAFELVSEFDQGDGPACAIIKHANPCGVARAETLTEAYRRAFDCDRTSAFGGIIALNQPLDGA
TAEEISSIFTEVVIAPGADAEAQEVFAKKKNLRLLTTDGLADPTAPGLAIRQVSGGYLVQDRDTGRLSADALQIVTKRAP
SDQEMADLMFAWTVAKHVKSNAIIYVKDGATVGVGAGQMSRVDSTRIAARKAQDMAEAMGLPAPLTQGSVVASDAFFPFA
DGLITAAEAGATALIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=608, Percent_Identity=35.6907894736842, Blast_Score=304, Evalue=1e-82,
Organism=Escherichia coli, GI1790439, Length=542, Percent_Identity=55.719557195572, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=36.1563517915309, Blast_Score=311, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI71985574, Length=307, Percent_Identity=31.5960912052117, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323768, Length=603, Percent_Identity=35.8208955223881, Blast_Score=298, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6323056, Length=605, Percent_Identity=35.2066115702479, Blast_Score=297, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24649832, Length=602, Percent_Identity=37.2093023255814, Blast_Score=320, Evalue=2e-87,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_ROSDO (Q16CE0)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681039.1
- ProteinModelPortal:   Q16CE0
- SMR:   Q16CE0
- STRING:   Q16CE0
- GeneID:   4198089
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_0655
- NMPDR:   fig|375451.6.peg.617
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q16CE0
- ProtClustDB:   PRK00881
- BioCyc:   RDEN375451:RD1_0655-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55892; Mature: 55760

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLAPLRRALLSVSDKTGLVALGKALAARGVELLSTGGTARALRDAGLEVKDVAEVTGF
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCCHHHHHHHCCC
PEMMDGRVKTLHPAVHGGLLALRDNDDHLAAMQEHGIAGIDLVVVNLYPFEQTVAKGAQY
HHHHCCCHHEECHHHHCCEEEEECCCCHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCHH
DEVIENIDIGGPAMIRSAAKNHGFVSVVVDVEDYDVLIAQLEAYDGHTTYALRQRLAQTA
HHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHEEEEHHHHCCCCHHHHHHHHHHHHH
YARTAAYDTAVSTWMAAQVGEPPRRRSFGGTLAQTLRYGENPHQDAAFYLDGSNSPGVAT
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHH
ARQLQGKELSYNNINDTDAAFELVSEFDQGDGPACAIIKHANPCGVARAETLTEAYRRAF
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DCDRTSAFGGIIALNQPLDGATAEEISSIFTEVVIAPGADAEAQEVFAKKKNLRLLTTDG
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCEEEEECC
LADPTAPGLAIRQVSGGYLVQDRDTGRLSADALQIVTKRAPSDQEMADLMFAWTVAKHVK
CCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
SNAIIYVKDGATVGVGAGQMSRVDSTRIAARKAQDMAEAMGLPAPLTQGSVVASDAFFPF
CCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCH
ADGLITAAEAGATALIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH
HCCHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLAPLRRALLSVSDKTGLVALGKALAARGVELLSTGGTARALRDAGLEVKDVAEVTGF
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEEECCCHHHHHHHCCCCHHHHHHHCCC
PEMMDGRVKTLHPAVHGGLLALRDNDDHLAAMQEHGIAGIDLVVVNLYPFEQTVAKGAQY
HHHHCCCHHEECHHHHCCEEEEECCCCHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCHH
DEVIENIDIGGPAMIRSAAKNHGFVSVVVDVEDYDVLIAQLEAYDGHTTYALRQRLAQTA
HHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCHHEEEEHHHHCCCCHHHHHHHHHHHHH
YARTAAYDTAVSTWMAAQVGEPPRRRSFGGTLAQTLRYGENPHQDAAFYLDGSNSPGVAT
HHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHH
ARQLQGKELSYNNINDTDAAFELVSEFDQGDGPACAIIKHANPCGVARAETLTEAYRRAF
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DCDRTSAFGGIIALNQPLDGATAEEISSIFTEVVIAPGADAEAQEVFAKKKNLRLLTTDG
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCEEEEECC
LADPTAPGLAIRQVSGGYLVQDRDTGRLSADALQIVTKRAPSDQEMADLMFAWTVAKHVK
CCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
SNAIIYVKDGATVGVGAGQMSRVDSTRIAARKAQDMAEAMGLPAPLTQGSVVASDAFFPF
CCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCH
ADGLITAAEAGATALIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH
HCCHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA