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The map label for this gene is rsmB [H]

Identifier: 110678030

GI number: 110678030

Start: 631240

End: 632511

Strand: Direct

Name: rsmB [H]

Synonym: RD1_0653

Alternate gene names: 110678030

Gene position: 631240-632511 (Clockwise)

Preceding gene: 110678028

Following gene: 110678031

Centisome position: 15.27

GC content: 64.78

Gene sequence:

>1272_bases
ATGGCAGACACAGGCCTTCCGGCGCGCAGAAGCGCTGTTTACCTGCTGGACCAGATCCTTGGGGAAGGCCGCCTGATGTC
GGAGCTTCTGACAGCGGGCACGCTTGACAAGCTGGAACCCGAAGACCGCGCGCGCGCAGCCCGACTGGCGCAGGACACCC
TGAGGGGGTTGGAGCGCGCCGATCGCATCTTGCAAAAACACCTGTCCAAATATCCGCCTTTGACGGTGCGCAATGCCTTG
CGGGTCGGAGCGGTGGAACTGTGCACCGGAACCGCCGCGCACGGCGTGGTGAACGCAATGGTGGAACTGGTATCGCGCCA
CAAACATTACAGTCACCTTAAAGGGCTGACGAATGCTGTTCTGCGCAAGGTCGCTGCGGACGGGCCCGCGGCATGGGATG
CTTTGCGCGCGCCCCGTCTGCCCAAGTGGCTGCGCGGCCCGCTGGTTGAGGCCTGGGGCGCGGATGCGATGGCGCGTATG
GAGATCGCGCATTTTGCCGGTGCGCCGCTTGATCTGACCGCGAAATCCGACGCCTCCGCTATCGCCGCGCTGACAGGTGG
TACAGTGTTGCCAACCGGGTCCGTGCGGGTTGAAAATGCAGGTCAGGTCTCGGCCATGCCGGGGTTTTCTGACGGAACAT
GGTGGGTGCAGGATGCCGCCGCCGCCTTGCCCGTGCGGATTCTGGCGCCGCAGCCCGGCGAAACCGTGCTTGATCTCTGC
GCAGCCCCCGGTGGCAAGACGATGCAACTGGCTGCCGCCGGTGCGCGGGTCACGGCGGTGGATGATTCTTCGGTGCGCAT
GGGCCGCGTCGCGGAAAACCTCGCGCGCACGGGCCTCAAAGCCGAGGTGCTGGTGCAGGATGCGCGCGCCACGTCGGGGA
CATTTGACGCGGTGTTGCTAGATGCGCCCTGTTCCGCGACGGGTACGATCCGCCGCCATCCCGATCTGCCCCATGCCAAG
GACGGGTCGGAGTTTGGCGATCTGATTGCCATGCAGTCTGAACTGATCGACCACGCCTGGGACCGCGTGAAACCCGGCGG
ACGGTTGGTATTTTGCACCTGTTCCCTGCTGCCTGATGAAGGCGAAGTGCAGGTGGACGAGGCGCTGGAACGGCATGAAG
ACATGCGCACGGATGCAGACGCCCTTGGTGTTCCCGGCGTAGATCCCGCGTGGCGCACCTCCGAGGGGGGGCTGCGATTG
CGGCCTGATTACTGGTCTGATCTGGGCGGTCTGGATGGATTTTACATCGCCGTGCTGCGCAAGGCCGGGTAA

Upstream 100 bases:

>100_bases
ACTGTCTTGGGTCATTTCGCTCTGTCCTCTGGCTTGCGTCTTGATATACGTCGCGCTGCGGGGCAGGCAAGGCACCCGTA
TGACAAGGAGCACCCGATAG

Downstream 100 bases:

>100_bases
CATCGCGGCCATATAAGGTTGCGGGTCGACAAAGAGCGGTGACTCGCAGCGGGCGGCGCGCTATCATGCCGCAAAGCGTC
GTCAGAACGCGTCAGGCAGA

Product: rRNA methyltransferase RsmB

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MADTGLPARRSAVYLLDQILGEGRLMSELLTAGTLDKLEPEDRARAARLAQDTLRGLERADRILQKHLSKYPPLTVRNAL
RVGAVELCTGTAAHGVVNAMVELVSRHKHYSHLKGLTNAVLRKVAADGPAAWDALRAPRLPKWLRGPLVEAWGADAMARM
EIAHFAGAPLDLTAKSDASAIAALTGGTVLPTGSVRVENAGQVSAMPGFSDGTWWVQDAAAALPVRILAPQPGETVLDLC
AAPGGKTMQLAAAGARVTAVDDSSVRMGRVAENLARTGLKAEVLVQDARATSGTFDAVLLDAPCSATGTIRRHPDLPHAK
DGSEFGDLIAMQSELIDHAWDRVKPGGRLVFCTCSLLPDEGEVQVDEALERHEDMRTDADALGVPGVDPAWRTSEGGLRL
RPDYWSDLGGLDGFYIAVLRKAG

Sequences:

>Translated_423_residues
MADTGLPARRSAVYLLDQILGEGRLMSELLTAGTLDKLEPEDRARAARLAQDTLRGLERADRILQKHLSKYPPLTVRNAL
RVGAVELCTGTAAHGVVNAMVELVSRHKHYSHLKGLTNAVLRKVAADGPAAWDALRAPRLPKWLRGPLVEAWGADAMARM
EIAHFAGAPLDLTAKSDASAIAALTGGTVLPTGSVRVENAGQVSAMPGFSDGTWWVQDAAAALPVRILAPQPGETVLDLC
AAPGGKTMQLAAAGARVTAVDDSSVRMGRVAENLARTGLKAEVLVQDARATSGTFDAVLLDAPCSATGTIRRHPDLPHAK
DGSEFGDLIAMQSELIDHAWDRVKPGGRLVFCTCSLLPDEGEVQVDEALERHEDMRTDADALGVPGVDPAWRTSEGGLRL
RPDYWSDLGGLDGFYIAVLRKAG
>Mature_422_residues
ADTGLPARRSAVYLLDQILGEGRLMSELLTAGTLDKLEPEDRARAARLAQDTLRGLERADRILQKHLSKYPPLTVRNALR
VGAVELCTGTAAHGVVNAMVELVSRHKHYSHLKGLTNAVLRKVAADGPAAWDALRAPRLPKWLRGPLVEAWGADAMARME
IAHFAGAPLDLTAKSDASAIAALTGGTVLPTGSVRVENAGQVSAMPGFSDGTWWVQDAAAALPVRILAPQPGETVLDLCA
APGGKTMQLAAAGARVTAVDDSSVRMGRVAENLARTGLKAEVLVQDARATSGTFDAVLLDAPCSATGTIRRHPDLPHAKD
GSEFGDLIAMQSELIDHAWDRVKPGGRLVFCTCSLLPDEGEVQVDEALERHEDMRTDADALGVPGVDPAWRTSEGGLRLR
PDYWSDLGGLDGFYIAVLRKAG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=245, Percent_Identity=35.9183673469388, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI76150623, Length=245, Percent_Identity=35.9183673469388, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI32698918, Length=221, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI40316918, Length=273, Percent_Identity=28.9377289377289, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI270288816, Length=235, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI8922322, Length=235, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI23199998, Length=235, Percent_Identity=31.9148936170213, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI270288818, Length=235, Percent_Identity=31.9148936170213, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367212, Length=393, Percent_Identity=36.6412213740458, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI87081985, Length=226, Percent_Identity=35.8407079646018, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=236, Percent_Identity=34.7457627118644, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71998419, Length=231, Percent_Identity=29.4372294372294, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=231, Percent_Identity=29.4372294372294, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17509941, Length=176, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=238, Percent_Identity=31.0924369747899, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319447, Length=178, Percent_Identity=30.8988764044944, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI22024126, Length=231, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24668781, Length=259, Percent_Identity=32.4324324324324, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356513, Length=259, Percent_Identity=32.4324324324324, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356579, Length=172, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 44965; Mature: 44834

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTGLPARRSAVYLLDQILGEGRLMSELLTAGTLDKLEPEDRARAARLAQDTLRGLERA
CCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRILQKHLSKYPPLTVRNALRVGAVELCTGTAAHGVVNAMVELVSRHKHYSHLKGLTNAV
HHHHHHHHHHCCCHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKVAADGPAAWDALRAPRLPKWLRGPLVEAWGADAMARMEIAHFAGAPLDLTAKSDASA
HHHHHCCCCHHHHHHCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCHH
IAALTGGTVLPTGSVRVENAGQVSAMPGFSDGTWWVQDAAAALPVRILAPQPGETVLDLC
HHHCCCCEEECCCCEEECCCCCEEECCCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHH
AAPGGKTMQLAAAGARVTAVDDSSVRMGRVAENLARTGLKAEVLVQDARATSGTFDAVLL
CCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
DAPCSATGTIRRHPDLPHAKDGSEFGDLIAMQSELIDHAWDRVKPGGRLVFCTCSLLPDE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
GEVQVDEALERHEDMRTDADALGVPGVDPAWRTSEGGLRLRPDYWSDLGGLDGFYIAVLR
CCEEHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHEE
KAG
CCC
>Mature Secondary Structure 
ADTGLPARRSAVYLLDQILGEGRLMSELLTAGTLDKLEPEDRARAARLAQDTLRGLERA
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DRILQKHLSKYPPLTVRNALRVGAVELCTGTAAHGVVNAMVELVSRHKHYSHLKGLTNAV
HHHHHHHHHHCCCHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKVAADGPAAWDALRAPRLPKWLRGPLVEAWGADAMARMEIAHFAGAPLDLTAKSDASA
HHHHHCCCCHHHHHHCCCCCCHHHHCCHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCHH
IAALTGGTVLPTGSVRVENAGQVSAMPGFSDGTWWVQDAAAALPVRILAPQPGETVLDLC
HHHCCCCEEECCCCEEECCCCCEEECCCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHH
AAPGGKTMQLAAAGARVTAVDDSSVRMGRVAENLARTGLKAEVLVQDARATSGTFDAVLL
CCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
DAPCSATGTIRRHPDLPHAKDGSEFGDLIAMQSELIDHAWDRVKPGGRLVFCTCSLLPDE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCC
GEVQVDEALERHEDMRTDADALGVPGVDPAWRTSEGGLRLRPDYWSDLGGLDGFYIAVLR
CCEEHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHHEE
KAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA