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The map label for this gene is rffA [H]

Identifier: 110677952

GI number: 110677952

Start: 553097

End: 554263

Strand: Direct

Name: rffA [H]

Synonym: RD1_0568

Alternate gene names: 110677952

Gene position: 553097-554263 (Clockwise)

Preceding gene: 110677950

Following gene: 110677953

Centisome position: 13.38

GC content: 57.93

Gene sequence:

>1167_bases
ATGGAATGGCGTATCCCGTTTAACAAACCAGCGCTTGTCGGCACTGAATCCGGCGAAGTGCAACAGGTTTTTGCGCAGGG
TAAATTTGCCGGCGGCGGCACGTTCAACAAGGCGTGCAACAGGTGGCTGCGGGATCACCTCGATGCCAGCGGTGTTCTGA
TTACGACGTCTTGCACCCATGCTTTGGAAATGGCGGCGTTGCTGTGTGATTTGCAGCCGCAGGATGAGGTCATCCTGCCG
TCCTATGCTTTTACCTCTACAGCAACCGCCTTCGCCCGTTGCGGAGCCAAGCTGGTTTTTGTCGATTGTGAAGCGGCGAC
GATGAACCTCGATGCGAATGCGGTTGCAGCAGCGATCACGCCGCGCAGCAAGGTGATCGTGGCTTTGCATTATGCCGGGG
TAAGCTGTGACATGCGTGCCTTGCGTGCGCTGGCCGATGAACATGGATTGAAGATCGTAGAGGATGCCGCACAGGCCCTG
TTTGCATCTTATGATGGCAAGCCCTGCGGGACGCTTGGGGACTACGGCTGTTTCAGCTTTCACGAAACGAAGAACATGCA
CTGCGGTGAGGGCGGCGCTTTGGTGCTCAACCGTCCCGAAGACGTGCAGAAGGCTGAGGTGCTCCTTGAGAAAGGAACCA
ATCGGATGCAGTTCTTCCGTGGTGAAACCTCCCGTTACGAATGGGTGGGCATGGGATCATCCTATGTGCCGAGTGAATTG
AACGCCGCCTTCCTTTTGGCGCAACTCAAGGCGGGCGCGCAGATCACGCAAGCGCGTCTGGCAAGCTGGCAGAGCTATGA
ACGACTGCTGCGCCCGCTCGAAGAGGCCGGACACGTCGCATTGCCGAACCCGCCACGTGATTGTCAGCACAATGCGCATA
TCTTCTGGATCAAGTTGGCGGGTAAGCCGGAGCGTGACGCGATGATCGCGCATCTGAAAGCGCAGGGGATAGCAGCGGTG
TTTCACTATATTCCCCTGCATTCCGCACCCGCTGGCAGGGTTTATGGCACCTTCCACGGGGAGGACATAAACACGACCCG
TGAAAGCGAACGGCTGCTGCGTCTGCCGCTTTACTACGCGATCACCCAAGAGGAGATCGCGGAGGTGGTGCATGCCGTCA
CAGCCTTCTTTGCCCTGACCGGAGAGACACACAGTGCCGTCGGCTGA

Upstream 100 bases:

>100_bases
ACACTTTTGAGGCGAAATGCGCCACTGGACACTTGCTATGACCTGTCTCATCGGTCGTTTGACTGTGGCGACAGTGTAAA
ATGTCATGATAGGACAATCC

Downstream 100 bases:

>100_bases
AGGTATGTCTGCACCCGTATTTCAGCCGGTCTTCCTGCTGTGTTCCGAGCGGTCCGGCAGCAATTTGTTTATCCGGCTTC
TGGACAGCCATCCCGTGTTC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MEWRIPFNKPALVGTESGEVQQVFAQGKFAGGGTFNKACNRWLRDHLDASGVLITTSCTHALEMAALLCDLQPQDEVILP
SYAFTSTATAFARCGAKLVFVDCEAATMNLDANAVAAAITPRSKVIVALHYAGVSCDMRALRALADEHGLKIVEDAAQAL
FASYDGKPCGTLGDYGCFSFHETKNMHCGEGGALVLNRPEDVQKAEVLLEKGTNRMQFFRGETSRYEWVGMGSSYVPSEL
NAAFLLAQLKAGAQITQARLASWQSYERLLRPLEEAGHVALPNPPRDCQHNAHIFWIKLAGKPERDAMIAHLKAQGIAAV
FHYIPLHSAPAGRVYGTFHGEDINTTRESERLLRLPLYYAITQEEIAEVVHAVTAFFALTGETHSAVG

Sequences:

>Translated_388_residues
MEWRIPFNKPALVGTESGEVQQVFAQGKFAGGGTFNKACNRWLRDHLDASGVLITTSCTHALEMAALLCDLQPQDEVILP
SYAFTSTATAFARCGAKLVFVDCEAATMNLDANAVAAAITPRSKVIVALHYAGVSCDMRALRALADEHGLKIVEDAAQAL
FASYDGKPCGTLGDYGCFSFHETKNMHCGEGGALVLNRPEDVQKAEVLLEKGTNRMQFFRGETSRYEWVGMGSSYVPSEL
NAAFLLAQLKAGAQITQARLASWQSYERLLRPLEEAGHVALPNPPRDCQHNAHIFWIKLAGKPERDAMIAHLKAQGIAAV
FHYIPLHSAPAGRVYGTFHGEDINTTRESERLLRLPLYYAITQEEIAEVVHAVTAFFALTGETHSAVG
>Mature_388_residues
MEWRIPFNKPALVGTESGEVQQVFAQGKFAGGGTFNKACNRWLRDHLDASGVLITTSCTHALEMAALLCDLQPQDEVILP
SYAFTSTATAFARCGAKLVFVDCEAATMNLDANAVAAAITPRSKVIVALHYAGVSCDMRALRALADEHGLKIVEDAAQAL
FASYDGKPCGTLGDYGCFSFHETKNMHCGEGGALVLNRPEDVQKAEVLLEKGTNRMQFFRGETSRYEWVGMGSSYVPSEL
NAAFLLAQLKAGAQITQARLASWQSYERLLRPLEEAGHVALPNPPRDCQHNAHIFWIKLAGKPERDAMIAHLKAQGIAAV
FHYIPLHSAPAGRVYGTFHGEDINTTRESERLLRLPLYYAITQEEIAEVVHAVTAFFALTGETHSAVG

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=375, Percent_Identity=52, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI145693159, Length=387, Percent_Identity=29.1989664082687, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42297; Mature: 42297

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWRIPFNKPALVGTESGEVQQVFAQGKFAGGGTFNKACNRWLRDHLDASGVLITTSCTH
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHH
ALEMAALLCDLQPQDEVILPSYAFTSTATAFARCGAKLVFVDCEAATMNLDANAVAAAIT
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEC
PRSKVIVALHYAGVSCDMRALRALADEHGLKIVEDAAQALFASYDGKPCGTLGDYGCFSF
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
HETKNMHCGEGGALVLNRPEDVQKAEVLLEKGTNRMQFFRGETSRYEWVGMGSSYVPSEL
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCCCHH
NAAFLLAQLKAGAQITQARLASWQSYERLLRPLEEAGHVALPNPPRDCQHNAHIFWIKLA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEEC
GKPERDAMIAHLKAQGIAAVFHYIPLHSAPAGRVYGTFHGEDINTTRESERLLRLPLYYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCHHHEE
ITQEEIAEVVHAVTAFFALTGETHSAVG
ECHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEWRIPFNKPALVGTESGEVQQVFAQGKFAGGGTFNKACNRWLRDHLDASGVLITTSCTH
CCCCCCCCCCCEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHH
ALEMAALLCDLQPQDEVILPSYAFTSTATAFARCGAKLVFVDCEAATMNLDANAVAAAIT
HHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCEEEEEC
PRSKVIVALHYAGVSCDMRALRALADEHGLKIVEDAAQALFASYDGKPCGTLGDYGCFSF
CCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
HETKNMHCGEGGALVLNRPEDVQKAEVLLEKGTNRMQFFRGETSRYEWVGMGSSYVPSEL
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCCCHH
NAAFLLAQLKAGAQITQARLASWQSYERLLRPLEEAGHVALPNPPRDCQHNAHIFWIKLA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEEEEEC
GKPERDAMIAHLKAQGIAAVFHYIPLHSAPAGRVYGTFHGEDINTTRESERLLRLPLYYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCHHHEE
ITQEEIAEVVHAVTAFFALTGETHSAVG
ECHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]