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The map label for this gene is menB [C]

Identifier: 110677865

GI number: 110677865

Start: 463338

End: 464144

Strand: Direct

Name: menB [C]

Synonym: RD1_0476

Alternate gene names: 110677865

Gene position: 463338-464144 (Clockwise)

Preceding gene: 110677864

Following gene: 110677867

Centisome position: 11.21

GC content: 58.24

Gene sequence:

>807_bases
ATGAAAGATATCCAGAACACGCTCGTCGAACTGCAGATCGAGGGCGGTATTGCGACGATAACGCTCAACAGCCCGCAAAC
TCTGAATGCGCTGTCCGAAGATATGCTGACGGGACTGCAAAACGCCCTGGACGGTATTGCCGTGAACCGTGATGTAAAGG
CCGTGATTTTACGCGGGGCAGGCAACCACTTTTGCGCCGGTCACAACCTCAAGGAAATGACCGCGCGCCGTGCAGATGCC
GACAAGGGATCCGCGTATTTCAATGCGCTCTTTGGGACCTGTTCGAAAATGATGATGTCGATTGTCGACCTGCCGCAGCC
CGTCATTGCCGAAGTCAAGGGTATCGCCACCGCCGCCGGGTGTCAGCTGGTTGCAACCTGCGATCTGGCGATTGCGGCGG
AGGATGCGCGCTTTGCCACGTCAGGCGTCAATATCGGACTGTTCTGTTCGACGCCGATGGTCGCGCTGTCGCGCAACGTC
CCGCGCAAAAAAGCGATGGAGATGCTGCTGCTGGGCGAGTTTCTGCCCGCGTCAAAAGCCGAGGAGATGGGACTGATCAA
CCGCGTCGTGCCCGCCGCCGACCTTGAAACGGCGAGCGCCGCGATGGCGCAGATCATCGTCGACAAATCACCCGTGGCGA
TCAAAACCGGCAAGAAAGCGTTTTATGAACAGTGCAACATGCCCCTTGATGCGGCCTATGCCTATGCTGGCGCTGTCATG
GCGGAAAACATGCTGGCGCGGGATGCGACCGAAGGGATCAACGCGTTTATCGACAAAAAGCCGATGCCGGACTGGACCGG
CGAATAG

Upstream 100 bases:

>100_bases
GCCGCAAAAACCGCAAAAAACCACGGCGGCGCGCGATATCACTGCTTGCAACTTGACCGGATGATCCGCTTTGATGTCGG
TATACGTCAGGGAGGCATCC

Downstream 100 bases:

>100_bases
GCCTCAAACGTCGCATGGCGGGCATTTTTTTGCCTGTGTACCCGCCACGCTCTGCTCAAACCTGATGGGTTAGCCGACCT
TGAAGCCCCAGAAATCCGTA

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKDIQNTLVELQIEGGIATITLNSPQTLNALSEDMLTGLQNALDGIAVNRDVKAVILRGAGNHFCAGHNLKEMTARRADA
DKGSAYFNALFGTCSKMMMSIVDLPQPVIAEVKGIATAAGCQLVATCDLAIAAEDARFATSGVNIGLFCSTPMVALSRNV
PRKKAMEMLLLGEFLPASKAEEMGLINRVVPAADLETASAAMAQIIVDKSPVAIKTGKKAFYEQCNMPLDAAYAYAGAVM
AENMLARDATEGINAFIDKKPMPDWTGE

Sequences:

>Translated_268_residues
MKDIQNTLVELQIEGGIATITLNSPQTLNALSEDMLTGLQNALDGIAVNRDVKAVILRGAGNHFCAGHNLKEMTARRADA
DKGSAYFNALFGTCSKMMMSIVDLPQPVIAEVKGIATAAGCQLVATCDLAIAAEDARFATSGVNIGLFCSTPMVALSRNV
PRKKAMEMLLLGEFLPASKAEEMGLINRVVPAADLETASAAMAQIIVDKSPVAIKTGKKAFYEQCNMPLDAAYAYAGAVM
AENMLARDATEGINAFIDKKPMPDWTGE
>Mature_268_residues
MKDIQNTLVELQIEGGIATITLNSPQTLNALSEDMLTGLQNALDGIAVNRDVKAVILRGAGNHFCAGHNLKEMTARRADA
DKGSAYFNALFGTCSKMMMSIVDLPQPVIAEVKGIATAAGCQLVATCDLAIAAEDARFATSGVNIGLFCSTPMVALSRNV
PRKKAMEMLLLGEFLPASKAEEMGLINRVVPAADLETASAAMAQIIVDKSPVAIKTGKKAFYEQCNMPLDAAYAYAGAVM
AENMLARDATEGINAFIDKKPMPDWTGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=45.4545454545455, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=29.6, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=25.4980079681275, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=25.4980079681275, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=25.4980079681275, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=25.9541984732824, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=25.0980392156863, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI62530384, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1788597, Length=258, Percent_Identity=32.5581395348837, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=30.6772908366534, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=29.6992481203008, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=29.5880149812734, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=27.7992277992278, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790281, Length=210, Percent_Identity=27.1428571428571, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=39.6887159533074, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=28.4, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=29.1338582677165, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.3653846153846, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=24.7058823529412, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=255, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=260, Percent_Identity=23.8461538461538, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=269, Percent_Identity=23.4200743494424, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=26.027397260274, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=37.1647509578544, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=28.8617886178862, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=28.8617886178862, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=29.9065420560748, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=25.9541984732824, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=194, Percent_Identity=28.8659793814433, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=25.4237288135593, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24650670, Length=273, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28418; Mature: 28418

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIQNTLVELQIEGGIATITLNSPQTLNALSEDMLTGLQNALDGIAVNRDVKAVILRGA
CCCHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECC
GNHFCAGHNLKEMTARRADADKGSAYFNALFGTCSKMMMSIVDLPQPVIAEVKGIATAAG
CCCEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
CQLVATCDLAIAAEDARFATSGVNIGLFCSTPMVALSRNVPRKKAMEMLLLGEFLPASKA
CEEEEEECEEEEECCCHHEECCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCH
EEMGLINRVVPAADLETASAAMAQIIVDKSPVAIKTGKKAFYEQCNMPLDAAYAYAGAVM
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHH
AENMLARDATEGINAFIDKKPMPDWTGE
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKDIQNTLVELQIEGGIATITLNSPQTLNALSEDMLTGLQNALDGIAVNRDVKAVILRGA
CCCHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECC
GNHFCAGHNLKEMTARRADADKGSAYFNALFGTCSKMMMSIVDLPQPVIAEVKGIATAAG
CCCEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
CQLVATCDLAIAAEDARFATSGVNIGLFCSTPMVALSRNVPRKKAMEMLLLGEFLPASKA
CEEEEEECEEEEECCCHHEECCCCEEEEECCCHHHHHCCCCHHHHHHHHHHHHHCCCCCH
EEMGLINRVVPAADLETASAAMAQIIVDKSPVAIKTGKKAFYEQCNMPLDAAYAYAGAVM
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHHHHHHHCCCCHHHHHHHHHHHH
AENMLARDATEGINAFIDKKPMPDWTGE
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA